Standard name
Human Ortholog
Description Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; role in misfolded protein quality control; forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP2 has a paralog, ERP4, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0.13 0.15 0.22 0.18 0.16 0.12 0.1 0.11 0.1 0.08 0.1 0.07 0.09 0.05 0.14 0.15 0.17 0 0 0.05 0.08 0.1 0.11
Bud 0 0 0 0 0 0 0.05 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.07 0.06 0 0.08 0.07 0.09 0.09 0.06 0.12 0.14 0.21 0.1 0.17 0 0 0 0 0 0
Cytoplasm 0.13 0.21 0.12 0.16 0.18 0.18 0.15 0.18 0.17 0.14 0.15 0.16 0.09 0.12 0.15 0.21 0.25 0.27 0 0.05 0 0 0 0
Endoplasmic Reticulum 0.82 0.76 0.75 0.62 0.6 0.65 0.43 0.42 0.46 0.4 0.34 0.34 0.88 0.77 0.71 0 0 0 0.84 0.83 0.85 0.71 0.71 0.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.07 0.05 0.08 0.2 0.09 0 0 0 0 0 0 0 0 0.08 0.07 0.33 0.31 0.35 0 0 0 0.07 0.08 0.09
Mitochondria 0 0 0.08 0.13 0.1 0.23 0.33 0.25 0.34 0.4 0.45 0.41 0 0 0 0.05 0.07 0.05 0 0 0 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.06 0.06 0 0.06 0 0.13 0.17 0.11 0.13 0.1 0.1 0 0.07 0.13 0.18 0.27 0.16 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 15 30 39 48 56 48 49 43 44 39 31 35 23 37 20 27 22 27 13 10 14 20 31 41
Bud 3 2 8 3 8 10 20 17 16 16 23 18 1 3 2 2 2 0 0 2 0 1 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 12 5 6 8 10 19 24 19 30 28 34 30 19 52 59 42 15 26 1 0 1 1 2 2
Cytoplasm 37 47 30 34 55 51 62 81 68 54 59 53 29 49 60 42 36 42 10 12 6 1 6 8
Endoplasmic Reticulum 236 172 194 132 182 190 177 183 181 154 129 116 288 330 289 7 1 4 260 208 232 190 219 275
Endosome 0 0 5 3 12 3 17 11 7 12 12 10 2 1 1 0 2 0 2 2 1 7 5 7
Golgi 19 11 20 42 28 11 17 19 16 15 11 7 14 36 29 65 45 55 10 4 6 18 23 34
Mitochondria 0 3 22 27 31 68 135 110 134 154 175 138 0 2 0 9 10 8 1 2 5 13 6 6
Nucleus 0 0 0 0 2 1 0 1 2 1 1 4 0 0 1 1 0 1 0 0 0 0 0 0
Nuclear Periphery 1 0 1 0 1 4 6 1 7 6 7 5 3 0 0 0 1 0 2 0 2 0 0 0
Nucleolus 0 0 0 0 1 1 1 8 3 7 5 9 0 2 1 3 4 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 2 0 0 3 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 1 0 0 1 0 0 0 2 0 2 1 1 0 0 0 0 0 0
Vac/Vac Membrane 27 14 16 6 19 9 52 73 42 49 37 33 13 28 54 36 39 25 5 3 0 6 5 7
Unique Cell Count 287 227 259 214 303 291 411 440 391 387 385 338 329 426 408 197 144 157 310 251 273 267 307 392
Labelled Cell Count 350 284 341 303 406 415 561 568 550 536 527 458 392 542 517 236 178 189 310 251 273 267 307 392


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.5 17.5 12.6 12.4 11.3 10.8 9.8 10.0 9.9 9.3 9.9 9.6 18.1 19.1 18.5 22.3 20.3 22.2 18.9 17.2 18.0
Std Deviation (1e-4) 3.6 3.9 3.0 3.1 2.5 2.3 2.5 2.5 2.0 2.6 2.5 2.3 4.3 4.9 5.0 6.1 7.2 6.0 6.4 5.5 4.3
Intensity Change (Log2) – – – -0.03 -0.16 -0.22 -0.36 -0.33 -0.36 -0.44 -0.36 -0.4 0.52 0.6 0.55 0.82 0.68 0.81 0.58 0.45 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 2.1 1.1 0.5 -1.2 -2.1 -1.4 -1.9 -2.8 -1.7 -3.2 -2.6 -4.5 -0.4 0.1 0.6
Bud 0 -0.3 0.2 1.1 0.5 0.7 0.7 1.7 1.3 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.9 0.7 2.4 2.1 1.4 2.9 2.7 3.4 3.3 2.1 4.5 5.2 6.6 3.5 5.3
Cytoplasm 1.4 2.2 2.0 1.3 2.4 2.0 0.9 1.3 1.4 -1.1 0 1.2 2.8 3.5 4.0
Endoplasmic Reticulum -3.1 -3.7 -2.5 -8.1 -8.5 -7.2 -8.8 -10.3 -9.8 4.0 0.8 -1.1 -15.2 -14.3 -14.3
Endosome 0 1.4 0 1.6 0.5 0 0.9 0.9 0.8 0 0 0 0 0 0
Golgi 3.8 0.6 -2.0 -2.0 -1.9 -2.0 -2.1 -2.8 -3.3 -1.8 0.3 -0.3 6.9 6.2 7.0
Mitochondria 1.5 0.7 4.7 7.2 5.4 7.5 8.8 10.0 8.8 -5.4 -5.5 -6.0 -1.6 -0.6 -1.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.7 0 -1.7 2.7 4.0 2.0 2.7 1.6 1.6 -1.2 0.2 2.9 4.0 5.9 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.9008 15.6997 15.9987 15.0653 13.5652 14.506 14.899 18.7202 17.5534 17.5862 14.6366 17.5776 13.2118 16.8643 15.5403 16.2998 14.2694 15.4584
Actin 0.0672 0.0223 0.0313 0.0003 0.0174 0.0042 0.045 0.0132 0.0262 0.0223 0.0286 0.02 0.0322 0.0075 0.0184 0.0029 0.0029 0.0057
Bud 0.0008 0.0003 0.001 0 0.0002 0.0001 0.0003 0.0001 0.0003 0.0001 0.0001 0.0001 0.0003 0.0001 0.0003 0 0.0001 0.0001
Bud Neck 0.0014 0.0005 0.0011 0.0003 0.0009 0.0017 0.0008 0.0005 0.0013 0.0005 0.0002 0.0013 0.0005 0.0003 0.0009 0.0002 0.0004 0.0012
Bud Periphery 0.0015 0.0009 0.0022 0 0.0006 0.0001 0.0004 0.0002 0.0007 0.0004 0.0001 0.0002 0.0005 0.0001 0.0004 0 0.0001 0.0003
Bud Site 0.0032 0.0063 0.0016 0 0.0002 0.0001 0.002 0.0072 0.0067 0.0002 0.0001 0.0001 0.0009 0.0103 0.0015 0 0.0004 0
Cell Periphery 0.0021 0.0036 0.002 0.0008 0.0014 0.0009 0.0016 0.0035 0.0035 0.0022 0.001 0.0016 0.0026 0.0037 0.002 0.002 0.001 0.0012
Cytoplasm 0.0379 0.0753 0.0373 0.0456 0.0343 0.0291 0.0197 0.0348 0.0276 0.0053 0.0141 0.0174 0.0179 0.0289 0.0276 0.0293 0.0068 0.014
Cytoplasmic Foci 0.029 0.0122 0.0306 0.0021 0.0068 0.0116 0.0303 0.0156 0.023 0.0048 0.0146 0.0145 0.0136 0.0132 0.0147 0.012 0.0188 0.0135
Eisosomes 0.0004 0.0007 0.0003 0 0.0003 0.0001 0.0007 0.0002 0.0006 0.0003 0.0003 0.0003 0.0009 0.0002 0.0002 0.0001 0.0002 0.0004
Endoplasmic Reticulum 0.614 0.735 0.6532 0.8998 0.7477 0.748 0.5694 0.67 0.6539 0.8134 0.7211 0.6422 0.6976 0.7365 0.7252 0.8042 0.6532 0.7391
Endosome 0.0528 0.0233 0.049 0.0104 0.0397 0.0416 0.0822 0.0401 0.055 0.0243 0.0598 0.0485 0.041 0.0281 0.0508 0.0283 0.0439 0.0403
Golgi 0.0683 0.0448 0.1043 0.0129 0.0545 0.102 0.1465 0.0739 0.1437 0.0863 0.0939 0.2127 0.1085 0.0794 0.1033 0.1003 0.1694 0.1464
Lipid Particles 0.0302 0.0399 0.0508 0.0185 0.0234 0.0416 0.0616 0.1284 0.0419 0.0302 0.0358 0.0303 0.0433 0.0768 0.0336 0.0135 0.0611 0.0274
Mitochondria 0.0156 0.0048 0.02 0.0002 0.0284 0.0019 0.006 0.0007 0.004 0.0013 0.001 0.0044 0.0099 0.0005 0.002 0.0004 0.0276 0.002
None 0.0043 0.0005 0.0007 0.0001 0.0029 0.0007 0.0021 0.0003 0.0007 0.0002 0.0004 0.0003 0.0062 0.0002 0.0005 0.0002 0.0003 0.0002
Nuclear Periphery 0.0346 0.0177 0.0063 0.0061 0.0211 0.007 0.0132 0.0065 0.0044 0.0045 0.0089 0.003 0.0128 0.005 0.0107 0.0043 0.003 0.0047
Nucleolus 0.0006 0.0002 0.0002 0 0.0004 0.0002 0.0007 0 0 0 0 0 0.0004 0.0001 0.0002 0 0.0003 0
Nucleus 0.0107 0.0023 0.0008 0.0004 0.0024 0.0007 0.0011 0.0004 0.0003 0.0001 0.0005 0.0002 0.0012 0.0002 0.0015 0.0002 0.0002 0.0002
Peroxisomes 0.0063 0.0003 0.0024 0 0.0054 0.0007 0.0043 0.0001 0.0013 0.0001 0.015 0.0003 0.0021 0.0003 0.0004 0.0002 0.0073 0.0004
Punctate Nuclear 0.0058 0.0006 0.0007 0.0001 0.0058 0.0048 0.0075 0.0002 0.0003 0.0001 0.0004 0.0002 0.0029 0.0003 0.0012 0.0002 0.0004 0.0002
Vacuole 0.0077 0.0067 0.0032 0.002 0.0046 0.0021 0.0029 0.0031 0.0029 0.0023 0.0031 0.0015 0.0028 0.0077 0.0032 0.0015 0.0017 0.0017
Vacuole Periphery 0.0057 0.0019 0.0011 0.0005 0.0016 0.001 0.0016 0.0009 0.0014 0.001 0.0009 0.0009 0.002 0.0006 0.0013 0.0004 0.0009 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 170.2785 135.4745 97.1393 100.9669 128.0486 110.6197 113.1224 111.6684 100.8552 142.9445
Translational Efficiency 1.3142 1.319 0.9626 1.183 1.3116 1.9358 1.3844 1.119 1.0919 1.2412

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; role in misfolded protein quality control; forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP2 has a paralog, ERP4, that arose from the whole genome duplication
Localization
Cell Percentages ER (98%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Erp2

Erp2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available