Standard name
Human Ortholog
Description Cystathionine gamma-lyase; catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.05 0.09 0.07 0.08 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.0 1.0 1.0 0.97 0.95 0.92 0.81 0.8 0.81 0.81 0.79 1.0 0.99 1.0 0.99 0.99 0.97 0.98 0.98 0.95 0.95 0.95 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.14 0.14 0.12 0.08 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 3 7 10 7 13 16 13 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 1 1 2 0 0 4 1 3 0 0 0 0 0 0 2 2 1 2 4 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 6 9 5 12 15 24 35 42 42 39 75 2 7 5 32 19 15 0 0 1 0 0 0
Cytoplasm 533 431 458 400 545 470 588 524 392 473 379 557 235 449 422 799 628 360 511 402 420 249 303 406
Endoplasmic Reticulum 0 1 2 1 12 13 31 89 67 72 35 89 0 3 1 15 18 13 0 0 9 1 0 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Golgi 0 0 0 0 0 1 1 10 7 0 3 6 0 0 0 3 1 0 0 0 0 0 0 0
Mitochondria 1 0 0 0 0 4 7 8 6 10 10 7 0 0 0 0 0 1 1 0 0 0 0 0
Nucleus 0 0 0 0 1 0 1 0 1 4 1 3 0 0 0 2 0 1 0 0 0 1 1 3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 1 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 1 1 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 3 2 6 7 5 12 18 1 3 2 8 1 4 0 0 2 2 3 7
Unique Cell Count 534 432 459 402 561 495 640 644 492 587 466 708 235 454 424 811 634 370 519 413 443 261 320 427
Labelled Cell Count 539 439 469 406 572 510 663 683 529 623 497 772 238 462 430 860 668 395 519 413 443 261 320 427


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 48.4 70.9 58.0 51.5 44.1 39.2 35.3 35.5 33.1 31.9 30.4 30.3 64.3 62.0 59.5 84.6 79.7 83.7 60.7 61.6 67.5
Std Deviation (1e-4) 8.0 13.1 10.6 8.9 9.4 8.3 8.1 8.8 7.7 7.3 7.5 6.5 15.3 12.2 11.4 25.3 22.3 27.3 15.4 16.5 17.8
Intensity Change (Log2) – – – -0.17 -0.4 -0.57 -0.72 -0.71 -0.81 -0.87 -0.93 -0.94 0.15 0.1 0.04 0.54 0.46 0.53 0.06 0.09 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 3.2 4.0 2.9 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.8 0.2 1.1 1.7 2.9 4.5 3.9 4.4 5.6 0 -0.5 -0.9 1.9 1.1 1.8
Cytoplasm -0.7 -3.3 -4.6 -6.0 -9.6 -10.1 -9.9 -9.6 -10.5 0.7 -1.7 -0.6 -2.1 -1.5 -3.1
Endoplasmic Reticulum 0 2.3 2.7 4.2 8.0 7.8 7.4 5.5 7.6 0 0 0 2.1 2.9 3.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 3.5 3.4 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 52.9067 62.532 55.8681 56.6252 49.6087 56.8194 59.8173 74.2318 68.0381 65.341 57.9244 66.8828 59.8195 73.4695 68.6467 63.3087 66.0776 67.0682
Actin 0.0006 0.0001 0.0002 0 0.0006 0.0001 0.0017 0.0003 0.0042 0.0013 0.0019 0.0003 0.0012 0.0004 0.0037 0.001 0.0001 0.0001
Bud 0.0004 0 0.0001 0.0001 0.0002 0 0.0008 0 0.0002 0.0002 0.0007 0.0002 0.001 0.0002 0.0003 0.0005 0 0
Bud Neck 0.0001 0.0001 0.0001 0.0002 0.0004 0.0009 0.0003 0.0001 0.0004 0.0003 0.0004 0.0007 0.0003 0.0001 0.0002 0.0003 0.0002 0.0003
Bud Periphery 0.0004 0 0 0 0.0004 0 0.0007 0 0.0002 0.0002 0.0021 0.0002 0.0005 0.0001 0.0002 0.0005 0 0
Bud Site 0.0002 0 0 0 0.0004 0 0.0006 0.0001 0.0018 0.0005 0.0009 0.0001 0.0003 0.0001 0.0003 0.0001 0 0
Cell Periphery 0.0007 0 0.0001 0 0.0012 0 0.001 0.0008 0.0002 0.0003 0.0048 0.0003 0.0004 0.0001 0.0002 0.0007 0 0
Cytoplasm 0.9652 0.9983 0.9852 0.9949 0.9485 0.9938 0.9176 0.9938 0.9674 0.9436 0.8384 0.9709 0.9456 0.996 0.9782 0.9436 0.9849 0.9908
Cytoplasmic Foci 0.0011 0.0001 0.0008 0.0002 0.0006 0.0003 0.0087 0.0001 0.0029 0.0037 0.0085 0.0011 0.0038 0.0002 0.0017 0.0009 0.0001 0.0002
Eisosomes 0.0001 0 0 0 0.0002 0 0.0002 0 0.0001 0.0006 0.0007 0.0001 0.0001 0 0.0001 0.0002 0 0
Endoplasmic Reticulum 0.0012 0.0001 0.0003 0.0002 0.0061 0.0004 0.0039 0.0003 0.0013 0.0014 0.0069 0.0014 0.0027 0.0003 0.0005 0.0045 0.0002 0.0004
Endosome 0.001 0 0.0006 0.0002 0.0024 0.0001 0.0035 0 0.003 0.0038 0.0082 0.0007 0.0056 0.0001 0.0021 0.0013 0.0001 0.0001
Golgi 0.0001 0 0.0001 0 0.0002 0 0.0007 0 0.0003 0.0007 0.0011 0.0001 0.0007 0 0.0008 0.0002 0 0
Lipid Particles 0.0007 0 0.0006 0 0.001 0 0.003 0.0003 0.0009 0.0035 0.0054 0.0003 0.0027 0 0.0008 0.0006 0 0
Mitochondria 0.0033 0 0.0002 0 0.0032 0 0.005 0 0.0007 0.0081 0.0128 0.0019 0.001 0 0.0021 0.0061 0 0
None 0.0028 0.0001 0.0028 0.0001 0.0029 0.0001 0.0074 0.0003 0.0029 0.0037 0.0172 0.0016 0.0035 0.0001 0.0017 0.0026 0.0001 0.0001
Nuclear Periphery 0.004 0.0002 0.0015 0.0007 0.0089 0.0008 0.0072 0.0007 0.004 0.0058 0.02 0.0039 0.0061 0.0005 0.0007 0.0162 0.0044 0.0017
Nucleolus 0.002 0 0.0011 0 0.0007 0 0.0024 0.0003 0.0004 0.0019 0.0075 0.0019 0.0015 0 0.0008 0.0012 0 0
Nucleus 0.0079 0.0009 0.0033 0.0023 0.0066 0.003 0.0145 0.0017 0.0048 0.014 0.0228 0.0102 0.014 0.0016 0.0032 0.0117 0.0093 0.0057
Peroxisomes 0.0001 0 0.0001 0 0 0 0.0005 0 0.0002 0.0004 0.0003 0 0.0005 0 0.0002 0.0002 0 0
Punctate Nuclear 0.0002 0 0.0021 0 0.0002 0 0.0082 0 0.0007 0.0029 0.0056 0.0006 0.0028 0 0.0007 0.0004 0.0001 0
Vacuole 0.0055 0.0001 0.0006 0.0007 0.0105 0.0003 0.0088 0.0009 0.0023 0.0014 0.0229 0.0024 0.0048 0.0003 0.0009 0.004 0.0003 0.0003
Vacuole Periphery 0.0024 0 0.0002 0.0002 0.0046 0.0001 0.0033 0 0.001 0.0015 0.0109 0.0012 0.0009 0 0.0004 0.0035 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 550.7711 523.5701 541.6927 504.2304 452.7253 482.065 434.2778 395.0713 461.0759 395.6871
Translational Efficiency 2.0632 2.0905 2.0057 2.2416 2.3631 2.4217 2.1479 2.6434 2.2545 2.3053

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cystathionine gamma-lyase; catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Cys3

Cys3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cys3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available