Standard name
Human Ortholog
Description Regulatory subunit A of the heterotrimeric PP2A complex; the heterotrimeric protein phosphatase 2A (PP2A) complex also contains regulatory subunit Cdc55p and either catalytic subunit Pph21p or Pph22p; required for cell morphogenesis and transcription by RNA polymerase III
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0.07 0 0.09 0 0 0 0.05 0.07 0.09 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.96 0.93 0.87 0.91 0.8 0.8 0.84 0.8 0.83 0.72 0.99 0.97 0.99 0.9 0.92 0.92 0.95 0.92 0.85 0.9 0.95 0.95
Endoplasmic Reticulum 0 0 0 0.08 0.1 0.06 0.15 0.14 0.09 0.1 0.06 0.13 0 0 0 0 0 0 0 0 0.05 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.06 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 1 1 2 1 4 0 0 0 0 0 1 0 1 0 0 0 0
Bud 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 1 0 1 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 5 1 0 5 6 6 6 2 12 5 19 5 5 11 13 19 22 1 0 0 0 0 0
Cytoplasm 203 216 26 86 152 140 168 183 118 139 107 146 203 181 250 243 239 223 192 208 49 90 205 246
Endoplasmic Reticulum 1 5 1 7 18 9 32 33 13 18 8 27 3 3 4 4 3 1 5 5 2 0 2 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 1 2 0 0 0 1 1 0 0 3 1 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 1 0 3 1 9 5 0 0 0 1 3 0 0 0 0 0 0 0
Nucleus 0 4 0 0 6 6 8 13 3 6 2 7 2 2 2 2 0 0 0 4 0 2 1 1
Nuclear Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 2 0 0 6 1 2 3 4 5 3 11 2 1 0 12 15 14 0 1 1 1 0 1
Unique Cell Count 205 221 27 92 175 154 210 230 141 173 129 204 206 186 253 269 259 242 203 227 58 100 216 258
Labelled Cell Count 211 232 28 93 188 162 219 242 144 184 135 220 216 192 267 283 281 262 203 227 58 100 216 258


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.6 30.0 25.3 21.4 23.2 21.6 21.3 20.8 17.8 19.0 17.2 17.8 25.9 25.9 26.0 31.5 35.2 33.1 20.1 24.1 24.8
Std Deviation (1e-4) 3.9 6.4 4.0 4.3 3.7 3.9 4.1 4.4 4.4 4.4 4.0 4.3 4.9 5.2 4.5 11.2 12.1 11.1 6.2 4.3 4.7
Intensity Change (Log2) – – – -0.24 -0.12 -0.23 -0.24 -0.28 -0.51 -0.41 -0.56 -0.51 0.03 0.04 0.04 0.32 0.48 0.39 -0.33 -0.07 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 -1.4 -0.9 -2.1 -2.1 -1.7 -2.0 -1.8 -2.8 0.8 0.3 1.0 -1.0 -0.8 -0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 18.8259 22.4886 20.7015 17.7874 17.7015 20.9938 19.0117 22.6614 19.2637 17.1412 14.8912 22.717 21.8717 27.4317 24.142 23.2978 21.0122 26.0595
Actin 0.0161 0.0007 0.0202 0.0379 0.0007 0.0146 0.0086 0.0006 0.0452 0.0224 0.0192 0.0049 0.0213 0.0007 0.0108 0.0138 0.0081 0.001
Bud 0.0003 0.0001 0.0009 0.0007 0.0002 0.0006 0.0009 0 0.0006 0.0014 0.0023 0.0003 0.0009 0.0001 0.0004 0.0007 0.0006 0.0002
Bud Neck 0.0012 0.0006 0.0025 0.0015 0.0032 0.0039 0.0005 0.0006 0.0059 0.0041 0.002 0.0037 0.0009 0.0005 0.0024 0.0019 0.0021 0.0029
Bud Periphery 0.0002 0 0.0009 0.0015 0.0001 0.0008 0.0004 0 0.0005 0.0012 0.0026 0.0004 0.0008 0 0.0004 0.0007 0.0007 0
Bud Site 0.0006 0.0002 0.0038 0.0027 0.0001 0.0013 0.0005 0.0003 0.0036 0.0011 0.0051 0.0002 0.0017 0.0004 0.0059 0.0226 0.0011 0
Cell Periphery 0.0001 0.0001 0.0004 0.0005 0.0001 0.0001 0.0001 0 0.0002 0.0004 0.0007 0.0002 0.0003 0 0.0002 0.0005 0.0001 0
Cytoplasm 0.8709 0.9645 0.8839 0.7529 0.8781 0.8329 0.8622 0.9563 0.8402 0.8795 0.6407 0.878 0.8195 0.9615 0.867 0.8942 0.7593 0.9315
Cytoplasmic Foci 0.035 0.0002 0.0059 0.008 0.0005 0.0065 0.006 0.0006 0.0108 0.0134 0.0183 0.001 0.0231 0.0007 0.0087 0.0088 0.009 0.0021
Eisosomes 0 0 0.0002 0.0008 0 0.0001 0 0 0.0002 0 0.0003 0.0001 0.0002 0 0.0004 0.0003 0.0002 0
Endoplasmic Reticulum 0.0053 0.001 0.0054 0.0029 0.0021 0.003 0.0139 0.0009 0.0067 0.0217 0.0387 0.0103 0.0096 0.0008 0.0028 0.0058 0.0036 0.0014
Endosome 0.0056 0 0.0037 0.0246 0.0002 0.013 0.0285 0.0001 0.02 0.025 0.0651 0.0026 0.0216 0.0001 0.0123 0.0074 0.0488 0.0014
Golgi 0.0053 0 0.0022 0.0156 0 0.0056 0.0042 0 0.0173 0.0055 0.0104 0.0012 0.0082 0 0.0022 0.004 0.0076 0.0005
Lipid Particles 0.0099 0 0.0035 0.0258 0 0.0169 0.0035 0 0.0018 0.0017 0.0197 0.0003 0.0235 0 0.0036 0.0035 0.0343 0.0027
Mitochondria 0.0009 0 0.0075 0.085 0.0001 0.003 0.0023 0 0.0067 0.0014 0.0256 0.0181 0.0092 0.0001 0.0159 0.0027 0.0489 0.0002
None 0.0023 0.0002 0.0008 0.0012 0.0003 0.0013 0.0035 0.0002 0.0024 0.0011 0.012 0.0003 0.0027 0.0006 0.0084 0.0013 0.0018 0.0003
Nuclear Periphery 0.0059 0.0028 0.0076 0.0046 0.0033 0.0078 0.0258 0.0027 0.004 0.0056 0.042 0.0085 0.0066 0.0028 0.0062 0.0028 0.022 0.0039
Nucleolus 0.0003 0 0.0003 0.0003 0.0002 0.0003 0.0006 0 0.0001 0.0001 0.0014 0.0001 0.0019 0 0.0008 0.0003 0.0013 0
Nucleus 0.0194 0.0292 0.0454 0.0226 0.1094 0.0665 0.0272 0.0364 0.0276 0.0086 0.0655 0.0663 0.0237 0.0304 0.0432 0.0253 0.0272 0.0504
Peroxisomes 0.0147 0 0.0018 0.0062 0 0.0165 0.0008 0 0.0019 0.0004 0.0042 0.0001 0.0152 0 0.0031 0.0007 0.0102 0.0002
Punctate Nuclear 0.0049 0.0003 0.001 0.001 0.0006 0.0039 0.0046 0.0009 0.0031 0.0019 0.0062 0.0017 0.0051 0.0009 0.0016 0.001 0.0037 0.0009
Vacuole 0.001 0.0002 0.0016 0.0021 0.0007 0.0011 0.0035 0.0001 0.0008 0.003 0.0122 0.001 0.0029 0.0001 0.0027 0.0013 0.0042 0.0002
Vacuole Periphery 0.0002 0 0.0006 0.0017 0.0001 0.0005 0.0025 0 0.0004 0.0007 0.0059 0.0006 0.0009 0 0.001 0.0003 0.0053 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 74.8904 82.198 62.1453 76.0889 71.9805 68.7801 70.0427 70.2055 77.3448 74.7424
Translational Efficiency 1.8843 1.7588 1.8469 1.6862 1.6135 2.4429 1.9925 1.7219 1.8439 1.8271

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Regulatory subunit A of the heterotrimeric PP2A complex; the heterotrimeric protein phosphatase 2A (PP2A) complex also contains regulatory subunit Cdc55p and either catalytic subunit Pph21p or Pph22p; required for cell morphogenesis and transcription by RNA polymerase III
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Tpd3

Tpd3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tpd3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available