Standard name
Human Ortholog
Description Component of the CCR4-NOT transcriptional complex; CCR4-NOT is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0.09 0.08 0.11 0 0 0 0 0 0
Cytoplasm 0.94 0.94 0.98 0.95 0.91 0.73 0.66 0.57 0.56 0.52 0.55 0.97 0.94 0.97 0.88 0.95 0.9 0.93 0.76 0.95 0.8 0.81 0.78
Endoplasmic Reticulum 0 0 0 0 0 0.07 0.07 0 0.09 0.06 0.09 0.05 0.05 0 0 0 0.07 0 0 0 0.13 0.11 0.13
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0.09 0.29 0.34 0.41 0.36 0.51 0.45 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0.12 0.09 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 8 1 4 7 5 10 0 1 1 0 0 0 1 3 0 0 0 0
Bud 1 0 0 0 0 1 6 0 3 7 8 1 0 0 0 0 0 1 0 1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 10 0 5 8 3 10 12 8 19 13 32 9 20 16 18 12 19 0 0 0 0 1 0
Cytoplasm 494 62 250 231 306 288 303 225 257 215 264 361 513 464 176 141 156 500 87 249 150 291 292
Endoplasmic Reticulum 19 0 3 6 6 27 32 11 40 23 45 19 26 19 7 2 12 13 3 8 25 39 50
Endosome 0 0 0 0 0 0 0 2 2 0 0 0 0 1 0 0 0 2 2 0 0 1 0
Golgi 0 0 0 0 2 0 1 0 0 2 1 0 2 0 0 0 0 0 1 0 0 4 3
Mitochondria 3 3 2 9 31 114 158 165 162 211 216 0 0 0 3 2 3 1 4 0 2 7 9
Nucleus 4 1 0 1 0 2 3 1 2 2 2 1 1 1 6 2 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 3 2 3 2 6 1 0 0 0 0 0 2 1 0 1 0 0
Nucleolus 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 14 2 0 1 9 1 22 48 41 23 17 0 3 3 6 2 5 6 1 0 4 6 14
Unique Cell Count 525 66 254 242 336 395 458 398 456 413 478 374 544 479 200 148 174 536 116 262 189 362 376
Labelled Cell Count 545 69 260 257 357 451 542 466 536 503 601 392 566 505 216 161 195 536 116 262 189 362 376


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.9 12.3 10.0 9.8 9.4 8.4 8.0 8.1 7.8 7.6 7.9 13.8 13.8 14.2 14.9 14.4 14.7 14.2 14.8 14.9
Std Deviation (1e-4) 2.4 3.2 1.3 1.2 1.4 1.4 1.5 1.6 1.4 1.5 1.6 1.9 1.7 1.8 2.4 2.5 2.3 1.9 2.2 2.2
Intensity Change (Log2) -0.02 -0.09 -0.24 -0.32 -0.31 -0.35 -0.38 -0.33 0.47 0.47 0.51 0.58 0.53 0.56 0.51 0.57 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.9 0 0.5 0.5 0 1.6 0.9 2.8 0.4 1.3 1.1 3.4 3.0 4.0
Cytoplasm -1.9 -3.8 -8.4 -9.9 -11.7 -11.9 -12.7 -12.2 -1.4 -2.6 -1.3 -4.6 -1.9 -4.0
Endoplasmic Reticulum 0 0 3.3 3.4 1.4 4.1 2.8 4.3 2.6 2.5 2.1 0 0 3.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.2 4.4 9.1 10.3 11.6 10.5 13.5 12.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.5 5.8 4.9 3.8 3.0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.2168 9.8347 8.9648 9.0311 8.3052 8.9193 10.2059 11.7855 10.7998 10.8371 10.1051 11.0867 5.7288 8.882 7.8777 7.5455 7.2981 7.3107
Actin 0.0068 0.0003 0.0008 0.0003 0.0014 0.0004 0.0111 0.0032 0.0076 0.001 0.001 0.0008 0.051 0.0028 0.0191 0.0456 0.0028 0.0168
Bud 0.0004 0.0005 0.0012 0.0006 0.0004 0.0009 0.0004 0.0005 0.0008 0.0005 0.0002 0.0003 0.0006 0.0004 0.0008 0.0022 0.0001 0.0018
Bud Neck 0.0005 0.0003 0.0005 0.0006 0.0007 0.0011 0.0003 0.0002 0.0007 0.0007 0.0006 0.0007 0.0004 0.0002 0.001 0.0009 0.0004 0.0011
Bud Periphery 0.0004 0.0005 0.0005 0.0003 0.0001 0.0003 0.0003 0.0001 0.0003 0.0001 0.0001 0.0001 0.0007 0.0001 0.0009 0.002 0.0001 0.0033
Bud Site 0.0004 0.0012 0.0043 0.0003 0.0004 0.0008 0.0005 0.0007 0.0008 0.0004 0.0002 0.0001 0.0025 0.0006 0.0008 0.0093 0.0002 0.0005
Cell Periphery 0.0004 0.0002 0.0003 0.0001 0.0001 0.0001 0.0003 0.0001 0.0002 0.0001 0 0.0001 0.0002 0.0001 0.0003 0.0001 0 0.0003
Cytoplasm 0.7957 0.9338 0.836 0.8054 0.8564 0.8439 0.8079 0.9475 0.8522 0.8321 0.7831 0.875 0.7361 0.9362 0.8206 0.7279 0.8169 0.8339
Cytoplasmic Foci 0.0188 0.0105 0.0234 0.0228 0.0261 0.0182 0.0182 0.0121 0.022 0.0295 0.0274 0.0192 0.0154 0.0083 0.0199 0.0312 0.0158 0.0221
Eisosomes 0.0003 0 0 0 0 0 0.0001 0 0 0 0 0 0.0005 0 0.0002 0.0001 0 0.0001
Endoplasmic Reticulum 0.0816 0.01 0.0231 0.0277 0.0095 0.0491 0.0658 0.0049 0.0208 0.0189 0.0089 0.031 0.0703 0.0177 0.0355 0.0142 0.0141 0.0251
Endosome 0.0588 0.0251 0.0767 0.0935 0.0691 0.0598 0.0617 0.0204 0.071 0.0883 0.1322 0.0509 0.0576 0.0227 0.0373 0.1203 0.0217 0.0396
Golgi 0.0075 0.0027 0.006 0.0066 0.0062 0.0058 0.0126 0.005 0.0069 0.012 0.0305 0.0086 0.0062 0.0029 0.0099 0.0111 0.0178 0.0169
Lipid Particles 0.0009 0 0.0002 0.0001 0.0048 0.0001 0.0004 0 0.0002 0.0001 0.0003 0 0.0062 0.0002 0.0021 0.0031 0.0687 0.0101
Mitochondria 0.0048 0.0028 0.0024 0.0039 0.0017 0.008 0.0058 0.0012 0.0034 0.0045 0.0065 0.0072 0.0065 0.0007 0.0023 0.005 0.0078 0.0153
None 0.0039 0.001 0.0014 0.0011 0.0033 0.001 0.0028 0.001 0.0014 0.001 0.0005 0.0009 0.014 0.0011 0.0227 0.0008 0.0006 0.0012
Nuclear Periphery 0.0037 0.001 0.0018 0.003 0.0024 0.0015 0.0026 0.0004 0.0026 0.001 0.0007 0.0009 0.0124 0.0011 0.0027 0.0024 0.0008 0.002
Nucleolus 0 0 0 0 0.0004 0 0 0 0 0 0 0 0.0004 0 0.0001 0.0001 0.0001 0.0001
Nucleus 0.0014 0.0011 0.0014 0.0015 0.0015 0.0016 0.0009 0.0004 0.001 0.0006 0.0004 0.0006 0.0032 0.0006 0.001 0.0011 0.0007 0.0008
Peroxisomes 0.0007 0.0001 0.0001 0.0001 0.001 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0.0044 0.0001 0.012 0.0104 0.028 0.0024
Punctate Nuclear 0.0013 0.0001 0.0003 0.0001 0.005 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0034 0.0001 0.0004 0.0008 0.0003 0.001
Vacuole 0.0092 0.0074 0.0154 0.0252 0.007 0.0049 0.0061 0.0015 0.0054 0.0061 0.0042 0.0022 0.0059 0.0032 0.0081 0.0077 0.0023 0.003
Vacuole Periphery 0.0024 0.0014 0.0042 0.0068 0.0024 0.0025 0.0018 0.0005 0.0022 0.0028 0.003 0.0014 0.0021 0.001 0.0025 0.0037 0.0008 0.0024

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 41.6959 31.3883 38.4309 43.986 43.8175 27.197 41.2576 39.2255 38.3652 49.8654
Translational Efficiency 1.2051 1.4495 1.0858 0.8463 1.1403 2.0786 1.0939 1.0845 1.1881 0.967

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Component of the CCR4-NOT transcriptional complex; CCR4-NOT is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Ccr4

Ccr4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ccr4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available