Standard name | |
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Human Ortholog | |
Description | Component of the CCR4-NOT transcriptional complex; CCR4-NOT is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 8 | 1 | 4 | – | 7 | 5 | 10 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 0 | 0 | 0 | 1 | 6 | 0 | – | 3 | 7 | 8 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 10 | 0 | 5 | 8 | 3 | 10 | 12 | 8 | – | 19 | 13 | 32 | 9 | 20 | 16 | 18 | 12 | 19 | 0 | 0 | 0 | 0 | 1 | 0 |
Cytoplasm | 494 | 62 | 250 | 231 | 306 | 288 | 303 | 225 | – | 257 | 215 | 264 | 361 | 513 | 464 | 176 | 141 | 156 | 500 | 87 | 249 | 150 | 291 | 292 |
Endoplasmic Reticulum | 19 | 0 | 3 | 6 | 6 | 27 | 32 | 11 | – | 40 | 23 | 45 | 19 | 26 | 19 | 7 | 2 | 12 | 13 | 3 | 8 | 25 | 39 | 50 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | – | 0 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 3 |
Mitochondria | 3 | 3 | 2 | 9 | 31 | 114 | 158 | 165 | – | 162 | 211 | 216 | 0 | 0 | 0 | 3 | 2 | 3 | 1 | 4 | 0 | 2 | 7 | 9 |
Nucleus | 4 | 1 | 0 | 1 | 0 | 2 | 3 | 1 | – | 2 | 2 | 2 | 1 | 1 | 1 | 6 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | – | 3 | 2 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 14 | 2 | 0 | 1 | 9 | 1 | 22 | 48 | – | 41 | 23 | 17 | 0 | 3 | 3 | 6 | 2 | 5 | 6 | 1 | 0 | 4 | 6 | 14 |
Unique Cell Count | 525 | 66 | 254 | 242 | 336 | 395 | 458 | 398 | 456 | 413 | 478 | 374 | 544 | 479 | 200 | 148 | 174 | 536 | 116 | 262 | 189 | 362 | 376 | |
Labelled Cell Count | 545 | 69 | 260 | 257 | 357 | 451 | 542 | 466 | 536 | 503 | 601 | 392 | 566 | 505 | 216 | 161 | 195 | 536 | 116 | 262 | 189 | 362 | 376 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.9 | 12.3 | 10.0 | 9.8 | 9.4 | 8.4 | 8.0 | 8.1 | – | 7.8 | 7.6 | 7.9 | 13.8 | 13.8 | 14.2 | 14.9 | 14.4 | 14.7 | 14.2 | 14.8 | 14.9 |
Std Deviation (1e-4) | 2.4 | 3.2 | 1.3 | 1.2 | 1.4 | 1.4 | 1.5 | 1.6 | – | 1.4 | 1.5 | 1.6 | 1.9 | 1.7 | 1.8 | 2.4 | 2.5 | 2.3 | 1.9 | 2.2 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.02 | -0.09 | -0.24 | -0.32 | -0.31 | – | -0.35 | -0.38 | -0.33 | 0.47 | 0.47 | 0.51 | 0.58 | 0.53 | 0.56 | 0.51 | 0.57 | 0.58 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.9 | 0 | 0.5 | 0.5 | 0 | – | 1.6 | 0.9 | 2.8 | 0.4 | 1.3 | 1.1 | 3.4 | 3.0 | 4.0 |
Cytoplasm | -1.9 | -3.8 | -8.4 | -9.9 | -11.7 | – | -11.9 | -12.7 | -12.2 | -1.4 | -2.6 | -1.3 | -4.6 | -1.9 | -4.0 |
Endoplasmic Reticulum | 0 | 0 | 3.3 | 3.4 | 1.4 | – | 4.1 | 2.8 | 4.3 | 2.6 | 2.5 | 2.1 | 0 | 0 | 3.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.2 | 4.4 | 9.1 | 10.3 | 11.6 | – | 10.5 | 13.5 | 12.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 3.5 | 5.8 | – | 4.9 | 3.8 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.2168 | 9.8347 | 8.9648 | 9.0311 | 8.3052 | 8.9193 | 10.2059 | 11.7855 | 10.7998 | 10.8371 | 10.1051 | 11.0867 | 5.7288 | 8.882 | 7.8777 | 7.5455 | 7.2981 | 7.3107 |
Actin | 0.0068 | 0.0003 | 0.0008 | 0.0003 | 0.0014 | 0.0004 | 0.0111 | 0.0032 | 0.0076 | 0.001 | 0.001 | 0.0008 | 0.051 | 0.0028 | 0.0191 | 0.0456 | 0.0028 | 0.0168 |
Bud | 0.0004 | 0.0005 | 0.0012 | 0.0006 | 0.0004 | 0.0009 | 0.0004 | 0.0005 | 0.0008 | 0.0005 | 0.0002 | 0.0003 | 0.0006 | 0.0004 | 0.0008 | 0.0022 | 0.0001 | 0.0018 |
Bud Neck | 0.0005 | 0.0003 | 0.0005 | 0.0006 | 0.0007 | 0.0011 | 0.0003 | 0.0002 | 0.0007 | 0.0007 | 0.0006 | 0.0007 | 0.0004 | 0.0002 | 0.001 | 0.0009 | 0.0004 | 0.0011 |
Bud Periphery | 0.0004 | 0.0005 | 0.0005 | 0.0003 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0009 | 0.002 | 0.0001 | 0.0033 |
Bud Site | 0.0004 | 0.0012 | 0.0043 | 0.0003 | 0.0004 | 0.0008 | 0.0005 | 0.0007 | 0.0008 | 0.0004 | 0.0002 | 0.0001 | 0.0025 | 0.0006 | 0.0008 | 0.0093 | 0.0002 | 0.0005 |
Cell Periphery | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0003 |
Cytoplasm | 0.7957 | 0.9338 | 0.836 | 0.8054 | 0.8564 | 0.8439 | 0.8079 | 0.9475 | 0.8522 | 0.8321 | 0.7831 | 0.875 | 0.7361 | 0.9362 | 0.8206 | 0.7279 | 0.8169 | 0.8339 |
Cytoplasmic Foci | 0.0188 | 0.0105 | 0.0234 | 0.0228 | 0.0261 | 0.0182 | 0.0182 | 0.0121 | 0.022 | 0.0295 | 0.0274 | 0.0192 | 0.0154 | 0.0083 | 0.0199 | 0.0312 | 0.0158 | 0.0221 |
Eisosomes | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0816 | 0.01 | 0.0231 | 0.0277 | 0.0095 | 0.0491 | 0.0658 | 0.0049 | 0.0208 | 0.0189 | 0.0089 | 0.031 | 0.0703 | 0.0177 | 0.0355 | 0.0142 | 0.0141 | 0.0251 |
Endosome | 0.0588 | 0.0251 | 0.0767 | 0.0935 | 0.0691 | 0.0598 | 0.0617 | 0.0204 | 0.071 | 0.0883 | 0.1322 | 0.0509 | 0.0576 | 0.0227 | 0.0373 | 0.1203 | 0.0217 | 0.0396 |
Golgi | 0.0075 | 0.0027 | 0.006 | 0.0066 | 0.0062 | 0.0058 | 0.0126 | 0.005 | 0.0069 | 0.012 | 0.0305 | 0.0086 | 0.0062 | 0.0029 | 0.0099 | 0.0111 | 0.0178 | 0.0169 |
Lipid Particles | 0.0009 | 0 | 0.0002 | 0.0001 | 0.0048 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0062 | 0.0002 | 0.0021 | 0.0031 | 0.0687 | 0.0101 |
Mitochondria | 0.0048 | 0.0028 | 0.0024 | 0.0039 | 0.0017 | 0.008 | 0.0058 | 0.0012 | 0.0034 | 0.0045 | 0.0065 | 0.0072 | 0.0065 | 0.0007 | 0.0023 | 0.005 | 0.0078 | 0.0153 |
None | 0.0039 | 0.001 | 0.0014 | 0.0011 | 0.0033 | 0.001 | 0.0028 | 0.001 | 0.0014 | 0.001 | 0.0005 | 0.0009 | 0.014 | 0.0011 | 0.0227 | 0.0008 | 0.0006 | 0.0012 |
Nuclear Periphery | 0.0037 | 0.001 | 0.0018 | 0.003 | 0.0024 | 0.0015 | 0.0026 | 0.0004 | 0.0026 | 0.001 | 0.0007 | 0.0009 | 0.0124 | 0.0011 | 0.0027 | 0.0024 | 0.0008 | 0.002 |
Nucleolus | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0014 | 0.0011 | 0.0014 | 0.0015 | 0.0015 | 0.0016 | 0.0009 | 0.0004 | 0.001 | 0.0006 | 0.0004 | 0.0006 | 0.0032 | 0.0006 | 0.001 | 0.0011 | 0.0007 | 0.0008 |
Peroxisomes | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0044 | 0.0001 | 0.012 | 0.0104 | 0.028 | 0.0024 |
Punctate Nuclear | 0.0013 | 0.0001 | 0.0003 | 0.0001 | 0.005 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0034 | 0.0001 | 0.0004 | 0.0008 | 0.0003 | 0.001 |
Vacuole | 0.0092 | 0.0074 | 0.0154 | 0.0252 | 0.007 | 0.0049 | 0.0061 | 0.0015 | 0.0054 | 0.0061 | 0.0042 | 0.0022 | 0.0059 | 0.0032 | 0.0081 | 0.0077 | 0.0023 | 0.003 |
Vacuole Periphery | 0.0024 | 0.0014 | 0.0042 | 0.0068 | 0.0024 | 0.0025 | 0.0018 | 0.0005 | 0.0022 | 0.0028 | 0.003 | 0.0014 | 0.0021 | 0.001 | 0.0025 | 0.0037 | 0.0008 | 0.0024 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 41.6959 | 31.3883 | 38.4309 | 43.986 | 43.8175 | 27.197 | 41.2576 | 39.2255 | 38.3652 | 49.8654 |
Translational Efficiency | 1.2051 | 1.4495 | 1.0858 | 0.8463 | 1.1403 | 2.0786 | 1.0939 | 1.0845 | 1.1881 | 0.967 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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