Standard name
Human Ortholog
Description Protein similar to GDP/GTP exchange factors; without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.12 0.13 0.05 0.05 0 0 0 0 0 0 0 0.13 0.26 0.19 0.17 0.21 0.18 0.13 0.09 0.16 0.17 0.15 0.26
Bud 0.1 0.14 0.15 0.16 0.24 0.15 0.1 0.12 0.12 0.13 0.09 0.11 0.07 0.17 0.18 0.16 0.13 0.12 0.22 0.23 0.13 0.15 0.13 0.14
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.18 0.11 0.19 0.15 0.12
Bud Site 0.06 0.05 0 0 0.08 0.05 0.05 0 0 0 0 0 0.05 0 0 0.05 0.06 0 – – – – – –
Cell Periphery 0.06 0 0 0 0 0 0 0 0 0 0 0 0.1 0.11 0.09 0.1 0.1 0.1 0 0 0 0 0 0.05
Cytoplasm 0.61 0.72 0.67 0.74 0.55 0.69 0.69 0.68 0.6 0.64 0.5 0.58 0.74 0.67 0.64 0.64 0.59 0.65 0.36 0.36 0.46 0.37 0.4 0.33
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.09 0.21 0.24 0.17 0.27 0.31 0.34 0.51 0.42 0.58 0.53 0 0.05 0.14 0 0 0.09 0.05 0 0.08 0 0.05 0
Nucleus 0.06 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.1 0 0.05 0 0.09 0 0.06 0.06 0 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 17 11 7 11 8 3 2 5 0 4 2 25 68 54 28 25 32 25 14 16 18 41 88
Bud 17 20 12 21 54 33 39 50 35 50 15 31 14 45 51 27 16 22 43 35 13 16 36 46
Bud Neck 0 0 2 1 3 4 2 7 5 6 2 3 0 0 0 0 5 0 26 28 11 20 40 39
Bud Site 10 8 3 4 18 10 18 7 11 6 5 9 9 7 3 8 7 4 – – – – – –
Cell Periphery 10 6 2 3 2 3 3 5 0 4 2 5 19 28 25 16 12 19 3 3 0 2 10 17
Cytoplasm 106 105 55 96 123 151 270 274 173 237 84 166 143 176 181 106 71 118 71 57 49 40 108 113
Endoplasmic Reticulum 1 3 1 0 0 2 2 0 1 0 1 2 3 4 3 17 7 4 4 0 0 0 1 3
Endosome 4 0 0 0 4 0 1 1 0 0 0 0 2 0 0 1 4 1 2 1 1 0 3 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 2 1 0 1 0 0 1 2
Mitochondria 19 13 17 31 39 59 122 137 148 156 97 153 2 12 40 7 5 16 9 5 8 2 14 12
Nucleus 10 1 0 1 12 2 8 14 3 13 2 7 1 1 1 5 0 4 0 1 0 0 0 0
Nuclear Periphery 2 2 1 0 0 1 2 2 0 3 3 0 1 0 2 0 0 0 1 0 0 0 0 0
Nucleolus 3 0 0 0 0 0 0 0 0 2 0 1 1 0 0 0 0 2 0 0 0 0 0 0
Peroxisomes 6 2 1 1 2 3 4 0 0 0 1 3 11 3 1 2 2 2 0 0 0 0 0 0
SpindlePole 18 1 4 0 21 5 23 23 4 18 2 5 5 3 1 4 6 8 1 0 0 1 0 2
Vac/Vac Membrane 5 2 0 1 1 2 2 4 2 5 2 2 0 0 2 3 4 6 6 7 1 2 9 5
Unique Cell Count 174 146 82 129 223 218 389 404 288 371 168 288 192 261 283 166 120 181 199 159 107 109 274 339
Labelled Cell Count 218 180 109 166 290 283 499 526 387 500 220 389 236 347 364 227 166 239 199 159 107 109 274 339


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 4.6 3.4 3.6 4.0 3.3 3.2 3.5 2.9 2.8 2.9 2.7 5.3 4.9 4.3 6.9 7.1 7.6 5.0 4.9 5.0
Std Deviation (1e-4) 1.0 1.3 0.8 1.3 1.9 1.0 1.4 1.7 1.0 1.0 0.9 1.2 1.2 1.2 1.1 1.5 1.9 2.1 1.3 1.3 1.1
Intensity Change (Log2) – – – 0.07 0.25 -0.02 -0.06 0.04 -0.24 -0.26 -0.23 -0.31 0.63 0.54 0.34 1.02 1.06 1.16 0.56 0.52 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9311 2.4984 2.0786 2.1772 1.7837 2.0948 1.6987 1.772 1.9384 1.9528 1.7614 1.8209 -0.4575 0.215 -0.1666 0.0628 -0.5245 0.261
Actin 0.0562 0.0227 0.0582 0.0113 0.182 0.0197 0.0295 0.0046 0.0638 0.1188 0.0428 0.0248 0.0155 0.0005 0.06 0.0072 0.0575 0.0354
Bud 0.0539 0.044 0.1811 0.0642 0.0271 0.0068 0.0306 0.0335 0.2237 0.0489 0.0412 0.0088 0.0452 0.0047 0.1119 0.0067 0.0185 0.0069
Bud Neck 0.0138 0.0067 0.0189 0.0012 0.008 0.1701 0.0265 0.0073 0.0094 0.004 0.0019 0.1055 0.0611 0.0019 0.0031 0.0078 0.0009 0.1986
Bud Periphery 0.2013 0.0656 0.5042 0.5955 0.3991 0.0486 0.0855 0.0414 0.4762 0.5064 0.3806 0.0489 0.101 0.0033 0.3754 0.0488 0.548 0.0155
Bud Site 0.0566 0.296 0.0691 0.0021 0.0046 0.0079 0.0875 0.2238 0.0335 0.0062 0.0083 0.0054 0.1254 0.4493 0.0345 0.0302 0.0015 0.0103
Cell Periphery 0.0298 0.018 0.0158 0.039 0.0653 0.0173 0.0071 0.0068 0.0044 0.0201 0.0224 0.0181 0.003 0.0026 0.0142 0.0266 0.0995 0.0044
Cytoplasm 0.2455 0.3308 0.0304 0.0769 0.1745 0.5388 0.3243 0.4499 0.0362 0.1364 0.1405 0.6487 0.2295 0.0411 0.0639 0.0995 0.0567 0.2399
Cytoplasmic Foci 0.0231 0.0053 0.0077 0.0018 0.0131 0.0298 0.0574 0.0171 0.0101 0.0191 0.0191 0.0169 0.0461 0.0022 0.0039 0.0103 0.0003 0.0147
Eisosomes 0.0017 0.0004 0.0007 0.0004 0.001 0.0003 0.0005 0.0003 0.0004 0.001 0.0006 0.0003 0.0005 0.0003 0.0028 0.0024 0.0031 0.0002
Endoplasmic Reticulum 0.0071 0.0031 0.0018 0.005 0.008 0.0042 0.0074 0.0024 0.0037 0.0033 0.0049 0.0045 0.004 0.0004 0.0019 0.0062 0.0013 0.0006
Endosome 0.0146 0.0017 0.0093 0.0015 0.005 0.0067 0.0446 0.0026 0.0127 0.0083 0.0182 0.0042 0.0411 0.0002 0.0009 0.0011 0.0001 0.0086
Golgi 0.0073 0.0019 0.0051 0.0005 0.0105 0.0032 0.011 0.0006 0.009 0.004 0.0099 0.0036 0.0065 0.0002 0.0009 0.0009 0.0003 0.0066
Lipid Particles 0.0126 0.0017 0.0018 0.0008 0.006 0.0047 0.0329 0.0004 0.0077 0.0153 0.0102 0.0007 0.0707 0.0002 0.0006 0.0025 0.0001 0.0023
Mitochondria 0.0152 0.0019 0.0057 0.001 0.0029 0.0031 0.0116 0.0008 0.0079 0.0185 0.0641 0.0026 0.0056 0.0001 0.0063 0.0033 0.0023 0.003
None 0.2277 0.1849 0.0793 0.1858 0.0752 0.1259 0.1733 0.194 0.0769 0.0417 0.2194 0.0965 0.1923 0.4914 0.3096 0.7374 0.2075 0.4383
Nuclear Periphery 0.005 0.0004 0.0012 0.0008 0.0012 0.0007 0.013 0.0004 0.006 0.0006 0.0017 0.0007 0.0058 0 0.0006 0.0007 0.0002 0.0001
Nucleolus 0.0012 0.0005 0.0005 0.0003 0.0002 0.0005 0.0023 0.0005 0.0007 0.0007 0.0004 0.0002 0.0045 0.0001 0.0008 0.0011 0.0001 0.0044
Nucleus 0.0034 0.0012 0.001 0.0009 0.0007 0.0009 0.0075 0.0013 0.0054 0.0005 0.0013 0.001 0.0029 0.0004 0.0013 0.0012 0.0003 0.0011
Peroxisomes 0.0128 0.0093 0.0032 0.0001 0.0106 0.0051 0.0153 0.001 0.0062 0.0406 0.0066 0.0018 0.0086 0.0001 0.0029 0.0007 0.0001 0.0061
Punctate Nuclear 0.0031 0.0004 0.0009 0.0002 0.0013 0.0016 0.0113 0.0023 0.0029 0.0006 0.0015 0.0009 0.0269 0.0001 0.0004 0.0007 0 0.0004
Vacuole 0.0076 0.0032 0.0032 0.0092 0.0035 0.0036 0.0179 0.0083 0.0023 0.0043 0.0032 0.0054 0.0027 0.0009 0.0036 0.0042 0.0016 0.0022
Vacuole Periphery 0.0006 0.0002 0.0009 0.0014 0.0003 0.0005 0.0028 0.0008 0.0009 0.0004 0.0011 0.0006 0.001 0 0.0006 0.0004 0.0002 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.4658 13.0509 6.9659 12.0577 11.955 15.8712 17.305 13.5157 11.0516 18.7916
Translational Efficiency 0.4303 0.5755 0.6545 0.3987 0.4454 0.543 0.4324 0.3655 0.4285 0.3707

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein similar to GDP/GTP exchange factors; without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
Localization
Cell Percentages cytoplasm (33%), bud neck (18%), mixed (19%)
Cell Cycle Regulation Yes
cytoplasm - T/G1 (3.5e-17)
bud neck - S/G2 (1.2e-11)
Subcompartmental Group N/A

Lte1

Lte1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lte1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available