Standard name
Human Ortholog
Description Trans-golgi network aminophospholipid translocase (flippase); maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation, endocytosis, protein trafficking between the Golgi and endosomal system and the cellular response to mating pheromone; autoinhibited by its C-terminal tail; localizes to the trans-Golgi network; mutations in human homolog ATP8B1 result in liver disease
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.15 0.23 0.11 0.05 0 0 0 0 0 0 0 0 0.14 0.13 0.07 – 0.29 – 0.1 0.12 0.14 0.14 0.14 0.1
Bud 0 0 0 0 0 0.05 0 0 0.06 0.06 0.09 0.06 0 0 0 – 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Cytoplasm 0 0.06 0 0.06 0.05 0 0 0 0 0 0 0 0.06 0.06 0 – 0.29 – 0 0 0 0 0 0
Endoplasmic Reticulum 0.06 0.14 0.06 0.05 0 0 0 0 0 0 0 0 0.13 0.11 0 – 0 – 0 0 0 0 0 0
Endosome 0.08 0 0.09 0.1 0.17 0 0.07 0.05 0 0 0 0 0.05 0.09 0.08 – 0.12 – 0.15 0.14 0.12 0.11 0.08 0.08
Golgi 0.7 0.55 0.59 0.26 0.15 0 0 0 0 0 0 0 0.64 0.68 0.69 – 0.35 – 0.65 0.61 0.62 0.63 0.67 0.68
Mitochondria 0.41 0.53 0.54 0.75 0.71 0.9 0.86 0.84 0.94 0.91 0.94 0.92 0.27 0.17 0.16 – 0.18 – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0.06 0.07 0.05 0.05 0.05 0.05 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0.05 0.05 0 0.07 0.05 0 0 0 0 0 0 0 – 0 – 0 0 0.07 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.09 – 0 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 68 62 46 13 4 6 7 19 10 4 10 10 16 21 12 – 5 – 52 40 67 43 50 41
Bud 2 9 5 6 13 23 22 30 26 31 50 32 1 2 2 – 0 – 4 4 1 1 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 1 5 7 7 6 2 14 12 0 0 0 – 0 – – – – – – –
Cell Periphery 1 2 3 1 3 1 7 2 1 1 3 0 0 1 0 – 0 – 0 0 0 0 0 0
Cytoplasm 16 15 10 15 15 14 14 18 8 15 10 8 7 10 7 – 5 – 2 3 1 1 2 0
Endoplasmic Reticulum 28 38 23 12 5 7 3 11 5 6 14 6 15 18 4 – 0 – 7 8 2 3 6 11
Endosome 36 5 37 26 52 16 47 40 4 6 6 12 6 14 13 – 2 – 78 44 60 33 28 34
Golgi 319 147 242 69 45 17 12 26 10 7 4 24 72 111 118 – 6 – 343 196 306 199 251 289
Mitochondria 185 142 222 196 220 422 589 615 401 452 530 511 30 28 27 – 3 – 7 8 11 11 13 14
Nucleus 2 2 0 0 0 0 0 3 4 3 0 3 1 0 1 – 0 – 0 0 0 0 0 0
Nuclear Periphery 1 1 4 4 2 4 6 10 5 7 10 12 0 0 0 – 0 – 0 1 1 0 0 1
Nucleolus 8 0 7 7 8 25 39 52 23 27 27 29 1 0 1 – 0 – 0 0 0 0 0 0
Peroxisomes 0 0 15 12 14 11 51 36 8 13 22 14 0 0 2 – 0 – 10 2 36 14 10 17
SpindlePole 1 0 5 2 3 8 11 12 2 3 5 5 0 2 3 – 0 – 3 1 1 1 2 1
Vac/Vac Membrane 26 8 9 4 8 6 22 12 3 14 3 10 4 15 16 – 0 – 12 10 4 2 3 3
Unique Cell Count 454 266 411 263 309 467 684 731 426 498 565 556 113 163 170 17 527 325 498 315 375 423
Labelled Cell Count 693 431 628 367 393 565 837 893 516 591 708 688 153 222 206 21 527 325 498 315 375 423


Early Golgi, Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.6 13.0 10.5 9.8 9.2 8.2 8.0 8.0 7.6 7.1 7.0 7.4 10.7 11.5 11.8 – 9.8 – 11.7 12.2 12.7
Std Deviation (1e-4) 1.8 2.2 1.7 1.7 1.8 1.7 1.8 1.8 1.7 1.5 1.7 1.8 2.0 2.0 2.2 – 2.2 – 1.8 1.8 1.8
Intensity Change (Log2) – – – -0.1 -0.2 -0.37 -0.4 -0.4 -0.48 -0.57 -0.59 -0.51 0.02 0.12 0.17 – -0.1 – 0.15 0.21 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.8 -5.2 -6.2 -7.6 -6.0 -5.1 -6.8 -6.2 -6.2 0.9 0.6 -1.5 – 0 –
Bud 0 2.5 3.1 2.1 2.7 3.7 3.9 5.1 3.6 0 0 0 – 0 –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Bud Site 0 0 0 0 0 0 0 3.2 3.0 0 0 0 – 0 –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Cytoplasm 2.2 1.8 0.5 -0.4 0 -0.6 0.5 -0.7 -1.1 0 2.2 0 – 0 –
Endoplasmic Reticulum -0.6 -2.7 -3.3 -5.4 -3.9 -3.6 -3.7 -2.5 -4.1 2.8 2.3 -1.7 – 0 –
Endosome 0.4 3.2 -3.5 -1.3 -2.3 -5.4 -5.5 -6.0 -4.8 -1.3 -0.2 -0.5 – 0 –
Golgi -8.3 -12.0 -17.9 -21.7 -21.2 -17.8 -19.3 -20.7 -18.8 0.9 2.0 2.4 – -1.9 –
Mitochondria 5.4 4.7 12.2 11.7 11.0 13.3 12.6 14.6 13.6 -5.2 -8.0 -8.5 – -2.9 –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Nuclear Periphery 0 0 0 0 0.6 0 0 1.0 1.4 0 0 0 – 0 –
Nucleolus 0.9 0.8 2.9 3.2 4.0 2.9 2.9 2.6 2.9 0 0 0 – 0 –
Peroxisomes 0.6 0.6 -1.1 2.6 1.0 -1.6 -0.9 0.2 -1.0 0 0 0 – 0 –
SpindlePole 0 0 0.6 0.5 0.6 0 0 0 0 0 0 0 – 0 –
Vacuole -0.6 0.3 -1.0 1.0 -0.7 -1.8 0.6 -2.3 -0.4 0 3.8 3.9 – 0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.5376 8.7902 8.4782 8.0707 7.3575 8.242 8.0191 10.1815 9.9369 9.3654 8.3437 9.6294 10.8668 11.356 10.3221 9.9648 10.9148 10.1762
Actin 0.0621 0.0197 0.0178 0.0045 0.0301 0.0298 0.0583 0.035 0.0187 0.0109 0.0091 0.0277 0.0239 0.1267 0.0713 0.0166 0.0008 0.0534
Bud 0.0007 0.0008 0.0003 0.0001 0.0003 0.0002 0.0006 0.0001 0.0002 0.0002 0.0002 0.0002 0.0003 0.0003 0.0006 0.0001 0 0.0002
Bud Neck 0.0021 0.0008 0.0005 0.0003 0.0007 0.0008 0.0006 0.0003 0.0006 0.0007 0.0008 0.0006 0.0009 0.0006 0.0014 0.0004 0.0001 0.0012
Bud Periphery 0.0009 0.0004 0.0002 0.0001 0.0003 0.0002 0.0007 0.0001 0.0002 0.0001 0.0002 0.0002 0.0002 0.0002 0.0005 0.0001 0 0.0001
Bud Site 0.0068 0.008 0.0004 0.0002 0.0004 0.0003 0.0013 0.0009 0.0005 0.0004 0.0004 0.0002 0.0006 0.0024 0.0062 0.0004 0 0.0003
Cell Periphery 0.0003 0.0002 0.0001 0 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0005 0 0.0001
Cytoplasm 0.0073 0.01 0.0023 0.0015 0.0027 0.0032 0.0021 0.0037 0.0014 0.0014 0.0016 0.002 0.0014 0.0304 0.0018 0.0117 0.0003 0.0012
Cytoplasmic Foci 0.1193 0.1633 0.0947 0.0883 0.0773 0.0733 0.0666 0.1583 0.1106 0.0838 0.1249 0.0633 0.1293 0.1755 0.1136 0.0903 0.069 0.065
Eisosomes 0.0003 0.0001 0.0001 0 0.0006 0.0001 0.0003 0 0.0003 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.0079 0.0026 0.0018 0.0005 0.002 0.0024 0.0019 0.0007 0.0004 0.0008 0.0014 0.002 0.0003 0.0072 0.0005 0.1055 0.0002 0.0006
Endosome 0.1968 0.2418 0.216 0.2763 0.2553 0.1985 0.1418 0.1917 0.1775 0.1326 0.2277 0.1562 0.1215 0.1225 0.1607 0.1863 0.1108 0.1322
Golgi 0.4134 0.4899 0.5639 0.5865 0.5818 0.6332 0.562 0.5505 0.6151 0.6974 0.5533 0.6918 0.5557 0.4588 0.5413 0.5519 0.809 0.709
Lipid Particles 0.0792 0.0262 0.0553 0.0173 0.0231 0.0241 0.0787 0.0441 0.0155 0.0543 0.0353 0.0159 0.0482 0.0202 0.0308 0.0257 0.0061 0.0131
Mitochondria 0.0289 0.0109 0.0164 0.0168 0.0107 0.0232 0.0247 0.0023 0.0251 0.0076 0.008 0.0201 0.0337 0.0054 0.0078 0.0031 0.0021 0.0062
None 0.0119 0.0001 0.0059 0 0.0019 0.0001 0.0027 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0005 0.0002 0 0
Nuclear Periphery 0.0124 0.0006 0.0013 0.0002 0.003 0.0008 0.0082 0.0002 0.0001 0.0003 0.0003 0.0004 0.0001 0.0026 0.0003 0.0017 0 0.0001
Nucleolus 0.0008 0.0005 0.0001 0 0.0001 0.0001 0.0011 0.0001 0 0 0 0 0.0001 0.0001 0.0004 0 0 0
Nucleus 0.004 0.0005 0.0003 0 0.0003 0.0002 0.0017 0.0001 0 0 0.0001 0.0001 0 0.0007 0.0003 0.0002 0 0
Peroxisomes 0.0306 0.0137 0.0164 0.0035 0.0039 0.0053 0.017 0.0097 0.032 0.0075 0.0333 0.016 0.0819 0.0433 0.0566 0.0025 0.001 0.0156
Punctate Nuclear 0.0071 0.0014 0.0025 0.0001 0.0015 0.0005 0.0259 0.0004 0.0002 0.0003 0.0006 0.0002 0.0004 0.0011 0.0032 0.0002 0 0.0001
Vacuole 0.0038 0.0068 0.0021 0.0014 0.0017 0.0016 0.0016 0.001 0.0006 0.0007 0.0011 0.0012 0.0005 0.0011 0.0011 0.0015 0.0001 0.0006
Vacuole Periphery 0.0032 0.0019 0.0019 0.0021 0.0023 0.0021 0.0021 0.0007 0.0007 0.0008 0.0014 0.0017 0.0007 0.0005 0.001 0.0011 0.0005 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 34.8543 21.0951 25.9092 32.4101 31.1232 24.3981 30.7067 30.6944 28.8487 39.0506
Translational Efficiency 0.4382 0.5653 0.5641 0.4582 0.4068 0.6291 0.4012 0.3507 0.3904 0.3473

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Trans-golgi network aminophospholipid translocase (flippase); maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation, endocytosis, protein trafficking between the Golgi and endosomal system and the cellular response to mating pheromone; autoinhibited by its C-terminal tail; localizes to the trans-Golgi network; mutations in human homolog ATP8B1 result in liver disease
Localization
Cell Percentages Golgi (30%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Drs2

Drs2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Drs2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available