Standard name | |
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Human Ortholog | |
Description | Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; protein overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 5 | 1 | 1 | 3 | 2 | 3 | 6 | 3 | 4 | 3 | 6 | 0 | 1 | 4 | 5 | 5 | 1 | 12 | 13 | 15 | 11 | 30 | 29 |
Bud | 1 | 2 | 5 | 0 | 1 | 3 | 3 | 4 | 15 | 5 | 5 | 5 | 6 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 1 | 1 | 3 | 2 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 10 | 7 | 4 | 7 | 10 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 26 | 52 | 38 | 16 | 30 | 15 | 15 | 19 | 18 | 13 | 14 | 19 | 28 | 59 | 75 | 66 | 60 | 53 | 24 | 17 | 30 | 35 | 80 | 41 |
Cytoplasm | 147 | 243 | 204 | 177 | 237 | 230 | 243 | 303 | 173 | 154 | 134 | 112 | 237 | 275 | 346 | 225 | 171 | 150 | 95 | 197 | 138 | 37 | 63 | 88 |
Endoplasmic Reticulum | 2 | 4 | 0 | 1 | 1 | 5 | 1 | 1 | 3 | 0 | 1 | 2 | 1 | 2 | 2 | 16 | 6 | 9 | 14 | 7 | 3 | 6 | 15 | 14 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 2 | 1 | 0 | 2 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 4 | 0 | 1 | 0 | 0 | 0 | 2 |
Mitochondria | 1 | 1 | 0 | 1 | 3 | 11 | 22 | 26 | 54 | 52 | 71 | 57 | 0 | 0 | 1 | 6 | 3 | 1 | 6 | 7 | 22 | 5 | 16 | 13 |
Nucleus | 0 | 1 | 0 | 0 | 1 | 1 | 2 | 2 | 1 | 2 | 3 | 2 | 0 | 0 | 0 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 11 | 4 | 8 | 0 | 12 | 3 | 2 | 4 | 2 |
Unique Cell Count | 153 | 256 | 206 | 182 | 248 | 248 | 265 | 321 | 203 | 195 | 171 | 161 | 239 | 277 | 357 | 261 | 196 | 171 | 167 | 272 | 227 | 104 | 227 | 214 |
Labelled Cell Count | 182 | 308 | 249 | 197 | 282 | 270 | 292 | 365 | 270 | 233 | 234 | 205 | 272 | 339 | 436 | 338 | 252 | 227 | 167 | 272 | 227 | 104 | 227 | 214 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 6.1 | 5.4 | 5.2 | 6.0 | 4.5 | 4.9 | 5.0 | 4.1 | 4.0 | 3.9 | 4.3 | 5.3 | 5.5 | 5.8 | 8.9 | 8.8 | 9.0 | 5.3 | 5.5 | 6.0 |
Std Deviation (1e-4) | 0.6 | 0.8 | 0.8 | 1.2 | 1.7 | 0.9 | 1.3 | 1.1 | 1.0 | 0.9 | 1.3 | 0.9 | 0.9 | 1.0 | 1.0 | 1.5 | 1.4 | 1.5 | 1.2 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.17 | -0.26 | -0.13 | -0.11 | -0.38 | -0.43 | -0.44 | -0.31 | -0.01 | 0.04 | 0.12 | 0.74 | 0.71 | 0.74 | -0.0 | 0.04 | 0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.1 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.7 | -1.9 | 0 | -4.4 | -4.5 | 0 | 0 | 0 | 0 | -2.0 | 0.8 | 0.7 | 1.8 | 2.8 | 2.8 |
Cytoplasm | -1.3 | -2.2 | -3.3 | -3.6 | -2.7 | -5.2 | -6.5 | -6.6 | -8.1 | 0.1 | 0.3 | -1.6 | -5.0 | -4.7 | -4.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 4.2 | 4.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.308 | 1.1629 | 0.8148 | 0.6397 | 0.9649 | 1.0273 | 0.7627 | 1.2838 | 1.0232 | 0.7847 | 1.0012 | 0.8079 | 0.8191 | 1.1538 | 0.6648 | 0.5795 | 0.3675 | 0.7512 |
Actin | 0.0465 | 0.0218 | 0.0258 | 0.0204 | 0.0233 | 0.0332 | 0.0632 | 0.0365 | 0.0229 | 0.0743 | 0.0378 | 0.0441 | 0.0561 | 0.025 | 0.0208 | 0.0014 | 0.0834 | 0.0182 |
Bud | 0.0005 | 0.0004 | 0.0012 | 0.0014 | 0.0124 | 0.0004 | 0.0015 | 0.0006 | 0.001 | 0.0009 | 0.0006 | 0.0011 | 0.0009 | 0.0007 | 0.0012 | 0.001 | 0.0014 | 0.0011 |
Bud Neck | 0.0021 | 0.0035 | 0.0029 | 0.0087 | 0.008 | 0.0137 | 0.0072 | 0.0023 | 0.0033 | 0.0013 | 0.0023 | 0.0111 | 0.0043 | 0.002 | 0.0029 | 0.002 | 0.008 | 0.0071 |
Bud Periphery | 0.0014 | 0.0011 | 0.0065 | 0.0047 | 0.043 | 0.003 | 0.0035 | 0.0013 | 0.002 | 0.0029 | 0.002 | 0.005 | 0.0037 | 0.0015 | 0.0035 | 0.0016 | 0.0043 | 0.0069 |
Bud Site | 0.0041 | 0.0373 | 0.0148 | 0.0036 | 0.0094 | 0.001 | 0.0142 | 0.052 | 0.0197 | 0.0018 | 0.0009 | 0.0011 | 0.008 | 0.0431 | 0.0113 | 0.0049 | 0.011 | 0.0011 |
Cell Periphery | 0.0313 | 0.0257 | 0.0196 | 0.0149 | 0.0041 | 0.0209 | 0.0309 | 0.019 | 0.0098 | 0.0031 | 0.0022 | 0.0239 | 0.0232 | 0.022 | 0.0151 | 0.0017 | 0.013 | 0.0207 |
Cytoplasm | 0.5263 | 0.6275 | 0.6568 | 0.6917 | 0.6686 | 0.6992 | 0.6174 | 0.747 | 0.7994 | 0.805 | 0.7814 | 0.7993 | 0.6649 | 0.6804 | 0.7146 | 0.746 | 0.8017 | 0.8071 |
Cytoplasmic Foci | 0.0197 | 0.0059 | 0.0102 | 0.008 | 0.0093 | 0.0092 | 0.0428 | 0.0053 | 0.0144 | 0.0081 | 0.0536 | 0.0103 | 0.0198 | 0.0052 | 0.0053 | 0.0118 | 0.0215 | 0.0066 |
Eisosomes | 0.002 | 0.002 | 0.0015 | 0.0006 | 0.0006 | 0.0013 | 0.0036 | 0.001 | 0.0003 | 0.0006 | 0.0005 | 0.0007 | 0.0017 | 0.0009 | 0.0009 | 0.0006 | 0.0011 | 0.0006 |
Endoplasmic Reticulum | 0.0144 | 0.0053 | 0.0051 | 0.011 | 0.003 | 0.0069 | 0.0076 | 0.0019 | 0.0025 | 0.0058 | 0.0029 | 0.0048 | 0.0046 | 0.0021 | 0.004 | 0.0034 | 0.0024 | 0.0034 |
Endosome | 0.023 | 0.0025 | 0.0144 | 0.0177 | 0.0273 | 0.0027 | 0.0289 | 0.0008 | 0.0072 | 0.0104 | 0.0378 | 0.0024 | 0.0153 | 0.0004 | 0.0012 | 0.0033 | 0.0026 | 0.0021 |
Golgi | 0.006 | 0.0049 | 0.0058 | 0.0147 | 0.0311 | 0.0021 | 0.0144 | 0.002 | 0.0051 | 0.0152 | 0.0097 | 0.0042 | 0.0057 | 0.0011 | 0.0021 | 0.0006 | 0.0052 | 0.0021 |
Lipid Particles | 0.0167 | 0.0008 | 0.0036 | 0.0031 | 0.0036 | 0.002 | 0.0289 | 0.0004 | 0.0045 | 0.0132 | 0.0073 | 0.0018 | 0.0078 | 0.0006 | 0.0046 | 0.0004 | 0.0007 | 0.0027 |
Mitochondria | 0.0058 | 0.0005 | 0.0063 | 0.1008 | 0.0159 | 0.0088 | 0.0042 | 0.0001 | 0.0008 | 0.002 | 0.0009 | 0.0065 | 0.0029 | 0.0002 | 0.0007 | 0.0002 | 0.0003 | 0.0023 |
None | 0.2586 | 0.2586 | 0.2133 | 0.0894 | 0.1286 | 0.1897 | 0.0959 | 0.1282 | 0.0968 | 0.0505 | 0.0258 | 0.0801 | 0.1557 | 0.2133 | 0.2 | 0.2159 | 0.0409 | 0.1149 |
Nuclear Periphery | 0.0089 | 0.0002 | 0.0016 | 0.0011 | 0.001 | 0.0025 | 0.0091 | 0.0001 | 0.0006 | 0.0015 | 0.0117 | 0.0002 | 0.0023 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0006 |
Nucleolus | 0.0008 | 0.0002 | 0.0004 | 0.0003 | 0.0007 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0.0009 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Nucleus | 0.0168 | 0.0004 | 0.0008 | 0.0009 | 0.0014 | 0.0007 | 0.003 | 0.0003 | 0.0008 | 0.0005 | 0.0044 | 0.0003 | 0.002 | 0.0003 | 0.0005 | 0.001 | 0.0002 | 0.0004 |
Peroxisomes | 0.0081 | 0.0002 | 0.002 | 0.0015 | 0.0015 | 0.0005 | 0.0168 | 0.0002 | 0.006 | 0.001 | 0.0069 | 0.002 | 0.0108 | 0.0002 | 0.0088 | 0.0001 | 0.0014 | 0.0004 |
Punctate Nuclear | 0.0027 | 0.0002 | 0.0039 | 0.0004 | 0.0008 | 0.0004 | 0.0025 | 0.0001 | 0.0008 | 0.0006 | 0.0079 | 0.0002 | 0.0068 | 0.0001 | 0.0007 | 0.0005 | 0.0002 | 0.0003 |
Vacuole | 0.0035 | 0.001 | 0.0029 | 0.0037 | 0.005 | 0.001 | 0.0028 | 0.0006 | 0.0019 | 0.001 | 0.0019 | 0.0008 | 0.0019 | 0.0007 | 0.0011 | 0.0028 | 0.0007 | 0.0012 |
Vacuole Periphery | 0.0008 | 0.0001 | 0.0005 | 0.0015 | 0.0014 | 0.0005 | 0.001 | 0 | 0.0002 | 0.0004 | 0.0008 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.5581 | 11.6654 | 7.0532 | 11.2896 | 7.833 | 6.7626 | 12.2317 | 13.7102 | 12.3894 | 13.0275 |
Translational Efficiency | 0.8702 | 0.4785 | 0.6818 | 0.5965 | 0.7599 | 0.7284 | 0.6653 | 0.5164 | 0.4824 | 0.4704 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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