Standard name
Human Ortholog
Description Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; protein overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.07 0.11 0.13 0.14
Bud 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.17 0.2 0.18 0.09 0.12 0.06 0.06 0.06 0.09 0.07 0.08 0.12 0.12 0.21 0.21 0.25 0.31 0.31 0.15 0.06 0.13 0.34 0.36 0.2
Cytoplasm 0.96 0.95 0.99 0.97 0.96 0.93 0.92 0.94 0.85 0.79 0.78 0.7 0.99 0.99 0.97 0.86 0.87 0.88 0.57 0.73 0.61 0.36 0.28 0.41
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0.08 0 0 0.06 0.07 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.08 0.08 0.27 0.27 0.42 0.35 0 0 0 0 0 0 0 0 0.1 0.05 0.07 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 5 1 1 3 2 3 6 3 4 3 6 0 1 4 5 5 1 12 13 15 11 30 29
Bud 1 2 5 0 1 3 3 4 15 5 5 5 6 1 5 0 0 0 0 0 0 0 1 0
Bud Neck 0 0 1 1 3 2 1 1 3 0 0 0 0 0 0 0 0 0 9 10 7 4 7 10
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cell Periphery 26 52 38 16 30 15 15 19 18 13 14 19 28 59 75 66 60 53 24 17 30 35 80 41
Cytoplasm 147 243 204 177 237 230 243 303 173 154 134 112 237 275 346 225 171 150 95 197 138 37 63 88
Endoplasmic Reticulum 2 4 0 1 1 5 1 1 3 0 1 2 1 2 2 16 6 9 14 7 3 6 15 14
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 0 2 1 0 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 4 0 1 0 0 0 2
Mitochondria 1 1 0 1 3 11 22 26 54 52 71 57 0 0 1 6 3 1 6 7 22 5 16 13
Nucleus 0 1 0 0 1 1 2 2 1 2 3 2 0 0 0 3 2 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 2 1 0 0 0 1 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 1 0 0 0 2 0 0 1 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 0 1 2 0 0 1 0 0 1 1 11 4 8 0 12 3 2 4 2
Unique Cell Count 153 256 206 182 248 248 265 321 203 195 171 161 239 277 357 261 196 171 167 272 227 104 227 214
Labelled Cell Count 182 308 249 197 282 270 292 365 270 233 234 205 272 339 436 338 252 227 167 272 227 104 227 214


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 6.1 5.4 5.2 6.0 4.5 4.9 5.0 4.1 4.0 3.9 4.3 5.3 5.5 5.8 8.9 8.8 9.0 5.3 5.5 6.0
Std Deviation (1e-4) 0.6 0.8 0.8 1.2 1.7 0.9 1.3 1.1 1.0 0.9 1.3 0.9 0.9 1.0 1.0 1.5 1.4 1.5 1.2 1.5 1.6
Intensity Change (Log2) -0.04 0.17 -0.26 -0.13 -0.11 -0.38 -0.43 -0.44 -0.31 -0.01 0.04 0.12 0.74 0.71 0.74 -0.0 0.04 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.7 -1.9 0 -4.4 -4.5 0 0 0 0 -2.0 0.8 0.7 1.8 2.8 2.8
Cytoplasm -1.3 -2.2 -3.3 -3.6 -2.7 -5.2 -6.5 -6.6 -8.1 0.1 0.3 -1.6 -5.0 -4.7 -4.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.6 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.2 4.2 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.308 1.1629 0.8148 0.6397 0.9649 1.0273 0.7627 1.2838 1.0232 0.7847 1.0012 0.8079 0.8191 1.1538 0.6648 0.5795 0.3675 0.7512
Actin 0.0465 0.0218 0.0258 0.0204 0.0233 0.0332 0.0632 0.0365 0.0229 0.0743 0.0378 0.0441 0.0561 0.025 0.0208 0.0014 0.0834 0.0182
Bud 0.0005 0.0004 0.0012 0.0014 0.0124 0.0004 0.0015 0.0006 0.001 0.0009 0.0006 0.0011 0.0009 0.0007 0.0012 0.001 0.0014 0.0011
Bud Neck 0.0021 0.0035 0.0029 0.0087 0.008 0.0137 0.0072 0.0023 0.0033 0.0013 0.0023 0.0111 0.0043 0.002 0.0029 0.002 0.008 0.0071
Bud Periphery 0.0014 0.0011 0.0065 0.0047 0.043 0.003 0.0035 0.0013 0.002 0.0029 0.002 0.005 0.0037 0.0015 0.0035 0.0016 0.0043 0.0069
Bud Site 0.0041 0.0373 0.0148 0.0036 0.0094 0.001 0.0142 0.052 0.0197 0.0018 0.0009 0.0011 0.008 0.0431 0.0113 0.0049 0.011 0.0011
Cell Periphery 0.0313 0.0257 0.0196 0.0149 0.0041 0.0209 0.0309 0.019 0.0098 0.0031 0.0022 0.0239 0.0232 0.022 0.0151 0.0017 0.013 0.0207
Cytoplasm 0.5263 0.6275 0.6568 0.6917 0.6686 0.6992 0.6174 0.747 0.7994 0.805 0.7814 0.7993 0.6649 0.6804 0.7146 0.746 0.8017 0.8071
Cytoplasmic Foci 0.0197 0.0059 0.0102 0.008 0.0093 0.0092 0.0428 0.0053 0.0144 0.0081 0.0536 0.0103 0.0198 0.0052 0.0053 0.0118 0.0215 0.0066
Eisosomes 0.002 0.002 0.0015 0.0006 0.0006 0.0013 0.0036 0.001 0.0003 0.0006 0.0005 0.0007 0.0017 0.0009 0.0009 0.0006 0.0011 0.0006
Endoplasmic Reticulum 0.0144 0.0053 0.0051 0.011 0.003 0.0069 0.0076 0.0019 0.0025 0.0058 0.0029 0.0048 0.0046 0.0021 0.004 0.0034 0.0024 0.0034
Endosome 0.023 0.0025 0.0144 0.0177 0.0273 0.0027 0.0289 0.0008 0.0072 0.0104 0.0378 0.0024 0.0153 0.0004 0.0012 0.0033 0.0026 0.0021
Golgi 0.006 0.0049 0.0058 0.0147 0.0311 0.0021 0.0144 0.002 0.0051 0.0152 0.0097 0.0042 0.0057 0.0011 0.0021 0.0006 0.0052 0.0021
Lipid Particles 0.0167 0.0008 0.0036 0.0031 0.0036 0.002 0.0289 0.0004 0.0045 0.0132 0.0073 0.0018 0.0078 0.0006 0.0046 0.0004 0.0007 0.0027
Mitochondria 0.0058 0.0005 0.0063 0.1008 0.0159 0.0088 0.0042 0.0001 0.0008 0.002 0.0009 0.0065 0.0029 0.0002 0.0007 0.0002 0.0003 0.0023
None 0.2586 0.2586 0.2133 0.0894 0.1286 0.1897 0.0959 0.1282 0.0968 0.0505 0.0258 0.0801 0.1557 0.2133 0.2 0.2159 0.0409 0.1149
Nuclear Periphery 0.0089 0.0002 0.0016 0.0011 0.001 0.0025 0.0091 0.0001 0.0006 0.0015 0.0117 0.0002 0.0023 0.0001 0.0003 0.0004 0.0001 0.0006
Nucleolus 0.0008 0.0002 0.0004 0.0003 0.0007 0.0002 0.0006 0.0001 0.0002 0.0001 0.0008 0.0001 0.0009 0.0001 0.0002 0.0002 0.0001 0.0001
Nucleus 0.0168 0.0004 0.0008 0.0009 0.0014 0.0007 0.003 0.0003 0.0008 0.0005 0.0044 0.0003 0.002 0.0003 0.0005 0.001 0.0002 0.0004
Peroxisomes 0.0081 0.0002 0.002 0.0015 0.0015 0.0005 0.0168 0.0002 0.006 0.001 0.0069 0.002 0.0108 0.0002 0.0088 0.0001 0.0014 0.0004
Punctate Nuclear 0.0027 0.0002 0.0039 0.0004 0.0008 0.0004 0.0025 0.0001 0.0008 0.0006 0.0079 0.0002 0.0068 0.0001 0.0007 0.0005 0.0002 0.0003
Vacuole 0.0035 0.001 0.0029 0.0037 0.005 0.001 0.0028 0.0006 0.0019 0.001 0.0019 0.0008 0.0019 0.0007 0.0011 0.0028 0.0007 0.0012
Vacuole Periphery 0.0008 0.0001 0.0005 0.0015 0.0014 0.0005 0.001 0 0.0002 0.0004 0.0008 0.0001 0.0006 0 0.0001 0.0002 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.5581 11.6654 7.0532 11.2896 7.833 6.7626 12.2317 13.7102 12.3894 13.0275
Translational Efficiency 0.8702 0.4785 0.6818 0.5965 0.7599 0.7284 0.6653 0.5164 0.4824 0.4704

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; protein overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate
Localization
Cell Percentages cytoplasm (32%), ER (3%), mixed (50%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Gip4

Gip4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gip4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available