Standard name
Human Ortholog
Description Translation initiation factor eIF5B; GTPase that promotes Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; promotes GTP-dependent maturation of 18S rRNA by Nob1p; protein abundance increases in response to DNA replication stress; homolog of bacterial IF2
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0.05 0.06 0.06 0.08 0.09 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.06 0.08 0.05 0.08 0.1 0.12 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.97 0.98 0.91 0.89 0.79 0.77 0.73 0.69 0.66 0.57 0.97 0.97 0.98 1.0 0.95 0.98 0.96 0.96 0.91 0.92 0.87 0.85
Endoplasmic Reticulum 0 0 0 0 0.08 0.07 0.15 0.13 0.15 0.14 0.14 0.12 0 0 0 0.06 0.08 0.05 0 0 0 0 0.08 0.09
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.12 0.14 0.21 0.27 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 2 6 23 28 19 30 25 32 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 1 2 0 0 0 1 1 2 0 6 0 0 0 0 0 0 0 1 3 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 3 6 10 13 20 40 25 25 40 31 40 4 1 2 1 1 0 0 0 0 0 0 0
Cytoplasm 190 206 395 306 304 289 402 372 237 273 174 169 194 146 181 48 69 40 185 200 395 101 126 152
Endoplasmic Reticulum 3 4 16 3 27 23 75 61 48 57 36 37 6 3 2 3 6 2 2 0 15 3 11 15
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1
Golgi 1 0 0 0 0 0 2 4 3 2 5 5 0 0 1 0 1 0 0 0 0 0 0 1
Mitochondria 0 0 0 0 2 6 12 21 38 56 55 80 0 0 2 0 1 1 0 0 2 1 0 0
Nucleus 0 0 2 1 0 2 1 2 0 2 1 1 0 0 0 0 0 0 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 1 0 1 1 1 6 0 3 1 4 0 0 0 1 3 0 1 3 8 0 0 1
Unique Cell Count 194 208 409 311 334 326 507 483 323 394 265 297 200 150 184 48 73 41 193 209 433 110 145 180
Labelled Cell Count 199 213 421 323 349 347 556 520 371 466 329 376 204 150 189 53 81 43 193 209 433 110 145 180


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 71.3 56.5 38.3 33.6 30.9 26.4 23.4 21.7 19.9 18.9 17.5 16.6 59.8 53.3 46.7 76.5 75.2 77.8 59.8 65.2 65.6
Std Deviation (1e-4) 16.2 13.9 8.3 8.0 6.8 6.1 5.1 4.9 4.7 4.5 4.3 4.2 12.9 13.3 12.2 24.6 21.2 27.0 19.6 20.6 20.1
Intensity Change (Log2) – – – -0.19 -0.31 -0.53 -0.71 -0.82 -0.94 -1.02 -1.13 -1.2 0.64 0.48 0.29 1.0 0.98 1.02 0.64 0.77 0.78


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 4.4 4.9 5.0 5.7 6.3 6.8 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.6 2.1 3.4 4.4 3.0 4.2 5.3 5.7 6.4 0 0 0 0 0 0
Cytoplasm 1.5 -3.2 -4.2 -7.7 -8.4 -9.1 -10.3 -10.8 -13.0 0.3 0.4 1.2 1.3 -0.9 0.3
Endoplasmic Reticulum -2.4 2.4 1.9 5.5 4.6 5.2 5.2 4.6 4.3 -0.6 -1.1 -1.9 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.1 4.3 7.1 7.9 9.6 11.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 48.8142 68.6527 64.5215 66.3663 54.8954 62.6437 50.6407 69.5537 65.3652 63.492 64.2093 62.9937 58.4381 57.2452 63.8025 56.1274 59.549 62.8418
Actin 0.0051 0.0002 0.0003 0.0001 0.0011 0.0003 0.0025 0.001 0.0002 0.0004 0.0023 0.0003 0.0044 0.0006 0.0011 0.0007 0.0008 0.0027
Bud 0.0011 0.0005 0.0006 0.001 0.001 0.0007 0.0009 0.0004 0.0005 0.0007 0.0002 0.0005 0.0007 0.0008 0.0018 0.0021 0.0004 0.0035
Bud Neck 0.0011 0.001 0.0008 0.0007 0.0015 0.002 0.0015 0.0005 0.0005 0.0008 0.0005 0.0015 0.0007 0.0004 0.0007 0.0005 0.001 0.0025
Bud Periphery 0.0019 0.0005 0.0006 0.0006 0.0026 0.0008 0.0017 0.0005 0.0004 0.0008 0.0002 0.0009 0.0008 0.0003 0.0007 0.0012 0.0002 0.0028
Bud Site 0.0007 0.0005 0.0004 0.0001 0.0005 0.0002 0.0012 0.0003 0.0003 0.0002 0.0001 0.0001 0.0006 0.0004 0.0004 0.0016 0.0001 0.0004
Cell Periphery 0.0179 0.0168 0.0147 0.0118 0.0167 0.0058 0.0222 0.0121 0.0103 0.0138 0.0058 0.0052 0.0063 0.0009 0.0011 0.0035 0.0003 0.0022
Cytoplasm 0.5 0.8136 0.7228 0.6802 0.6798 0.7057 0.6657 0.8862 0.8506 0.7122 0.7833 0.8376 0.7779 0.9556 0.9133 0.7391 0.9178 0.8133
Cytoplasmic Foci 0.0076 0.0007 0.0038 0.0011 0.0095 0.0058 0.005 0.0008 0.0029 0.0019 0.0015 0.0028 0.0047 0.0026 0.0047 0.0034 0.0063 0.0067
Eisosomes 0.0002 0 0 0 0.0008 0.0001 0.0006 0 0 0 0 0 0.0002 0 0 0.0002 0 0
Endoplasmic Reticulum 0.1399 0.0376 0.0616 0.009 0.0597 0.133 0.1049 0.0295 0.0404 0.027 0.0135 0.0732 0.0763 0.0169 0.0186 0.0052 0.0175 0.0526
Endosome 0.013 0.002 0.0052 0.0073 0.0093 0.0118 0.0076 0.0022 0.0043 0.0066 0.013 0.0109 0.0125 0.0039 0.01 0.0165 0.0139 0.0287
Golgi 0.0015 0.0002 0.0003 0.0001 0.0009 0.001 0.0015 0.0011 0.0005 0.0005 0.0019 0.0013 0.0014 0.0011 0.001 0.0004 0.0013 0.0055
Lipid Particles 0.0042 0.0009 0.0023 0.0004 0.0055 0.0019 0.0054 0.0015 0.0018 0.0022 0.0026 0.0005 0.0019 0.0001 0.0013 0.0007 0.0001 0.0002
Mitochondria 0.0078 0.0003 0.0004 0.0003 0.009 0.0047 0.0089 0.0012 0.0013 0.0003 0.0016 0.0017 0.0025 0.0008 0.0006 0.005 0.0005 0.0079
None 0.0045 0.0001 0.0011 0.0001 0.0055 0.0019 0.0048 0.0003 0.0015 0.0003 0.0003 0.0003 0.0035 0.0002 0.0003 0.0033 0.0003 0.0005
Nuclear Periphery 0.0459 0.009 0.0114 0.0206 0.0143 0.0142 0.0195 0.0032 0.0044 0.0067 0.0181 0.0075 0.0121 0.0015 0.004 0.0071 0.0053 0.0086
Nucleolus 0.0024 0.0001 0.0002 0.0002 0.0024 0.0011 0.0069 0.0001 0.0009 0.0003 0.0008 0.0001 0.0002 0 0 0.0033 0 0.0005
Nucleus 0.0139 0.0031 0.003 0.0058 0.0058 0.0039 0.0093 0.0015 0.0023 0.003 0.0036 0.0018 0.0034 0.0012 0.0016 0.0061 0.0017 0.0043
Peroxisomes 0.0003 0 0.0001 0 0.0006 0.0002 0.0014 0 0.0001 0 0.0001 0 0.0012 0 0.0001 0.0002 0.0001 0.0001
Punctate Nuclear 0.0048 0 0.001 0 0.0009 0.0002 0.0018 0 0.0003 0.0001 0.0005 0 0.005 0 0.0001 0.0002 0 0.0001
Vacuole 0.1754 0.1047 0.1488 0.206 0.1353 0.0844 0.1105 0.0542 0.0706 0.2064 0.1223 0.0446 0.0703 0.0108 0.0316 0.1429 0.0238 0.0401
Vacuole Periphery 0.0507 0.0081 0.0205 0.0544 0.0374 0.0204 0.016 0.0031 0.0059 0.0158 0.0277 0.0092 0.0137 0.0017 0.0071 0.0568 0.0084 0.0168

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 264.7584 309.4513 299.6817 225.3595 255.3136 273.0413 232.2615 213.8614 229.2232 203.7688
Translational Efficiency 2.026 1.9246 1.5184 2.3683 1.7554 1.5116 1.5482 1.6834 1.3272 1.5556

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Translation initiation factor eIF5B; GTPase that promotes Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; promotes GTP-dependent maturation of 18S rRNA by Nob1p; protein abundance increases in response to DNA replication stress; homolog of bacterial IF2
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Fun12

Fun12


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fun12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available