Standard name | |
---|---|
Human Ortholog | |
Description | Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 1 | 0 | 0 | 1 | 0 | 4 | 3 | 1 | 2 | 6 | 2 | 1 | 5 | 1 | 1 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | 143 | 98 | 76 | 145 | 161 | 235 | 229 | 141 | 184 | 159 | 141 | 97 | 195 | 207 | 183 | 178 | 107 | 35 | 136 | 95 | 72 | 158 | 193 |
Endoplasmic Reticulum | – | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 1 | 1 | 3 | 4 | 0 | 0 | 1 | 9 | 19 | 8 | 0 | 0 | 0 | 0 | 0 | 3 |
Endosome | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 |
Nuclear Periphery | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Unique Cell Count | 143 | 99 | 76 | 145 | 161 | 236 | 233 | 142 | 185 | 162 | 147 | 98 | 197 | 209 | 188 | 180 | 107 | 37 | 139 | 99 | 77 | 165 | 203 | |
Labelled Cell Count | 144 | 99 | 76 | 146 | 161 | 239 | 236 | 143 | 187 | 168 | 147 | 98 | 201 | 209 | 199 | 201 | 119 | 37 | 139 | 99 | 77 | 165 | 203 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 322.8 | 301.7 | 336.1 | 354.8 | 301.6 | 287.8 | 263.0 | 260.1 | 277.1 | 269.6 | 259.7 | 411.1 | 409.2 | 380.4 | 281.6 | 332.7 | 386.4 | 321.1 | 323.3 | 320.9 |
Std Deviation (1e-4) | – | 68.5 | 64.3 | 69.1 | 62.6 | 57.8 | 63.3 | 61.3 | 45.2 | 68.2 | 67.8 | 60.7 | 74.8 | 90.5 | 92.1 | 100.2 | 104.7 | 108.4 | 89.1 | 78.7 | 91.5 |
Intensity Change (Log2) | – | – | – | 0.16 | 0.23 | -0.0 | -0.07 | -0.2 | -0.21 | -0.12 | -0.16 | -0.22 | 0.45 | 0.44 | 0.33 | -0.1 | 0.14 | 0.36 | 0.09 | 0.1 | 0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.9 | 1.2 | 1.3 | 0.6 | -0.5 | 0.3 | 0.5 | -0.5 | -1.4 | 0 | 0 | 0 | -0.9 | -0.1 | 1.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0019 | 0.0001 | 0.0005 | 0.0002 | 0.0003 | 0.0072 | 0.0093 | 0.0002 | 0.0067 | 0.0001 | 0.0012 | 0.0006 | – | – | – | – | – | – |
Bud | 0.0005 | 0.0002 | 0.0006 | 0.0001 | 0.0158 | 0.0141 | 0.0022 | 0.0002 | 0.002 | 0.0001 | 0.0005 | 0.0005 | – | – | – | – | – | – |
Bud Neck | 0.0001 | 0.0001 | 0.0002 | 0.0009 | 0.0027 | 0.0028 | 0.0006 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0003 | – | – | – | – | – | – |
Bud Periphery | 0.0002 | 0 | 0.0004 | 0 | 0.0033 | 0.0129 | 0.0008 | 0.0001 | 0.001 | 0 | 0.0003 | 0.0005 | – | – | – | – | – | – |
Bud Site | 0.0001 | 0 | 0.0005 | 0 | 0.0019 | 0.0006 | 0.0005 | 0.0002 | 0.0018 | 0 | 0.001 | 0.0001 | – | – | – | – | – | – |
Cell Periphery | 0.0002 | 0 | 0.0038 | 0 | 0.0035 | 0.0006 | 0.0007 | 0.0001 | 0.0033 | 0 | 0.0023 | 0.0005 | – | – | – | – | – | – |
Cytoplasm | 0.7431 | 0.9941 | 0.6665 | 0.9913 | 0.2255 | 0.498 | 0.794 | 0.963 | 0.829 | 0.9959 | 0.7485 | 0.8517 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0003 | 0.0001 | 0.0079 | 0.0001 | 0.0769 | 0.0014 | 0.0065 | 0.0002 | 0.0006 | 0 | 0.0055 | 0.0003 | – | – | – | – | – | – |
Eisosomes | 0 | 0 | 0.0012 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0021 | 0 | 0.0039 | 0 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0005 | 0.0037 | 0.0015 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | – | – | – | – | – | – |
Endosome | 0.0005 | 0 | 0.0006 | 0 | 0.0014 | 0.0095 | 0.0114 | 0 | 0.0005 | 0 | 0.0002 | 0.0003 | – | – | – | – | – | – |
Golgi | 0 | 0 | 0.0003 | 0 | 0.0002 | 0.0004 | 0.0018 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | – | – | – | – | – | – |
Lipid Particles | 0 | 0 | 0.0069 | 0 | 0.0014 | 0.0001 | 0.0055 | 0 | 0.0007 | 0 | 0.0052 | 0.0001 | – | – | – | – | – | – |
Mitochondria | 0.0134 | 0.0003 | 0.1412 | 0.0002 | 0.1531 | 0.0128 | 0.0117 | 0.0302 | 0.0589 | 0.0001 | 0.1294 | 0.1023 | – | – | – | – | – | – |
None | 0.0029 | 0.0002 | 0.0089 | 0.0001 | 0.0947 | 0.0028 | 0.0121 | 0.0002 | 0.002 | 0.0001 | 0.0032 | 0.0005 | – | – | – | – | – | – |
Nuclear Periphery | 0.074 | 0.0005 | 0.0014 | 0.0013 | 0.0019 | 0.1331 | 0.0502 | 0.0002 | 0.0097 | 0.0002 | 0.0005 | 0.0013 | – | – | – | – | – | – |
Nucleolus | 0.0037 | 0 | 0.0991 | 0 | 0.1535 | 0.0023 | 0.0046 | 0.0013 | 0.0134 | 0 | 0.0759 | 0.0045 | – | – | – | – | – | – |
Nucleus | 0.1468 | 0.0042 | 0.0122 | 0.0056 | 0.2005 | 0.1967 | 0.063 | 0.0032 | 0.0473 | 0.0034 | 0.0093 | 0.0105 | – | – | – | – | – | – |
Peroxisomes | 0.0001 | 0 | 0.0038 | 0 | 0.0044 | 0.0012 | 0.0018 | 0.0004 | 0.0024 | 0 | 0.0036 | 0.0004 | – | – | – | – | – | – |
Punctate Nuclear | 0.0002 | 0 | 0.0002 | 0 | 0.0025 | 0.0015 | 0.004 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 | – | – | – | – | – | – |
Vacuole | 0.0016 | 0 | 0.0133 | 0.0001 | 0.0328 | 0.0419 | 0.0025 | 0.0001 | 0.0066 | 0 | 0.0027 | 0.0036 | – | – | – | – | – | – |
Vacuole Periphery | 0.0099 | 0 | 0.0305 | 0.0001 | 0.023 | 0.0565 | 0.0152 | 0.0003 | 0.0108 | 0 | 0.0059 | 0.022 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5559.235 | 5704.3125 | 6612.2392 | 5958.9495 | 6301.1657 | 6058.3944 | 6199.2673 | 5803.0844 | 6765.1497 | 5909.4939 |
Translational Efficiency | 1.505 | 1.54 | 1.4475 | 1.473 | 1.4786 | 1.4128 | 1.5977 | 1.739 | 1.5462 | 1.762 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|