Standard name
Human Ortholog
Description Putative calcium channel involved in calcium release under hypotonic stress; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.25 0.37 0.11 0.18 0.12 0.09 0.08 0.11 – 0.06 0.12 0.12 0.5 0.24 0.14 0.15 0.15 0.37 0.24 0.23 0.37 0.1 0.25 0.22
Bud 0.11 0.18 0.14 0.18 0.18 0.15 0.12 0.13 – 0.12 0.12 0.11 0.12 0.13 0 0.14 0.06 0.2 0.21 0.22 0.14 0.29 0.26 0.24
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.14 0.15 0.1 0.29 0.14 0.08
Bud Site 0.07 0.07 0.08 0.05 0.05 0.07 0 0 – 0 0 0 0.06 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.37 0.39 0.31 0.31 0.22 0.19 0.19 0.18 – 0.23 0.21 0.22 0.31 0.6 0.79 0.45 0.42 0.28 0.1 0.09 0 0 0.1 0.13
Endoplasmic Reticulum 0.13 0.1 0.05 0.11 0.11 0.11 0.11 0.08 – 0.09 0.08 0.12 0.31 0.13 0.09 0.05 0.08 0.05 0.05 0 0.07 0 0 0.05
Endosome 0 0 0.09 0.05 0.13 0.12 0.14 0.17 – 0.15 0.07 0.09 0 0 0 0 0.1 0.1 0 0 0 0.06 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0.21 0.14 0.19 0.16 0.43 0.38 0.4 – 0.48 0.58 0.49 0.12 0 0 0.12 0.12 0.12 0.11 0.12 0.14 0.09 0.08 0.14
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.12 0.07 0.3 0.17 0.25 0.11 0.13 0.08 – 0.06 0 0.05 0 0 0 0.07 0.07 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0 0.14 0.12 0.15 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 63 79 36 40 32 38 39 70 – 35 56 60 8 40 19 44 21 46 80 59 160 21 78 66
Bud 28 39 48 41 50 60 56 83 – 64 55 56 2 22 6 40 9 24 71 57 59 62 80 75
Bud Neck 0 1 0 0 0 0 0 0 – 0 1 1 0 1 0 3 3 1 48 40 45 61 44 23
Bud Site 17 15 27 12 13 28 17 23 – 21 15 14 1 1 0 7 4 0 – – – – – –
Cell Periphery 6 2 13 13 2 8 8 3 – 15 10 17 0 7 3 4 2 3 2 1 3 0 2 4
Cytoplasm 92 84 103 69 60 78 90 113 – 127 96 110 5 99 110 128 61 34 35 24 17 5 29 39
Endoplasmic Reticulum 32 21 16 24 29 44 51 49 – 50 37 62 5 22 13 13 11 6 17 10 30 3 12 14
Endosome 10 1 30 12 36 50 64 108 – 81 33 46 0 0 1 11 15 12 14 10 17 13 9 7
Golgi 0 0 2 1 3 2 3 2 – 1 2 8 0 0 0 1 0 1 5 5 13 1 2 3
Mitochondria 30 45 46 42 44 179 176 248 – 261 265 246 2 0 0 34 17 15 38 31 61 19 26 42
Nucleus 3 1 3 3 2 3 5 7 – 3 3 0 1 3 2 7 4 0 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 2 0 0 2 – 1 3 1 0 0 0 0 0 0 2 1 1 0 1 1
Nucleolus 0 0 1 1 0 12 10 33 – 14 15 9 0 0 0 0 1 0 0 0 1 1 0 0
Peroxisomes 31 14 100 38 68 45 60 53 – 32 17 26 0 0 1 20 10 5 2 0 7 3 4 1
SpindlePole 10 3 18 7 5 8 9 13 – 9 6 6 0 4 0 40 18 18 5 2 4 3 4 1
Vac/Vac Membrane 9 0 11 3 10 7 12 25 – 12 2 12 0 0 1 3 3 3 13 13 6 12 10 23
Unique Cell Count 250 213 337 222 272 413 465 624 549 457 504 16 164 140 285 144 123 338 264 437 214 308 310
Labelled Cell Count 332 305 454 306 356 562 600 832 726 616 674 24 199 156 355 179 168 338 264 437 214 308 310


Ambiguous, Bud, Bud Neck, Cell Periphery, Late Golgi, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 5.4 5.5 6.2 6.2 5.9 6.0 6.2 – 6.0 6.4 6.6 5.8 6.5 7.0 4.9 5.3 6.4 6.5 6.9 7.0
Std Deviation (1e-4) 1.0 0.9 1.3 2.2 1.5 1.4 1.6 1.5 – 1.3 1.4 1.4 0.6 0.8 1.1 1.0 0.9 1.2 1.4 1.6 2.2
Intensity Change (Log2) – – – 0.18 0.18 0.1 0.13 0.18 – 0.13 0.21 0.26 0.09 0.25 0.34 -0.16 -0.05 0.23 0.24 0.32 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud 1.3 1.4 0.1 -0.9 -0.4 – -1.1 -0.9 -1.4 – -0.3 -3.1 -0.1 -2.5 1.4
Bud Neck 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0
Bud Site -1.2 -1.6 -0.6 -2.7 -2.9 – -2.7 -2.9 -3.5 – -3.4 -3.4 -3.0 -2.1 -3.2
Cell Periphery 1.1 -2.5 -1.6 -1.9 -3.9 – -0.9 -1.4 -0.4 – 0.2 0 -1.9 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum 2.7 2.8 3.0 3.1 1.8 – 2.4 1.9 3.7 – 3.4 1.9 -0.1 1.3 0.1
Endosome -1.5 1.7 1.4 2.1 3.5 – 2.6 -0.9 0.1 – -3.9 -3.3 -2.5 0.5 0.3
Golgi 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0
Mitochondria 1.7 0.9 8.8 7.6 8.4 – 10.3 12.7 10.5 – -5.0 -4.6 -0.6 -0.5 -0.4
Nuclear Periphery 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus 0 0 2.7 0 4.0 – 2.5 3.0 0 – 0 0 0 0 0
Peroxisomes -3.4 -1.3 -6.5 -5.9 -8.6 – -9.7 -10.2 -9.8 – -7.8 -7.0 -7.1 -5.4 -5.8
SpindlePole -1.2 -2.3 -2.5 -2.6 -2.7 – -3.1 -3.3 -3.5 – -1.5 -2.8 3.7 2.7 3.3
Vac – – – – – – – – – – – – – – –
Cortical Patches 2.5 0.4 -0.7 -1.1 0.3 – -2.3 0.7 0.5 – 4.0 0.9 1.8 1.2 6.6
Cytoplasm 0.1 -2.4 -3.7 -3.7 -4.4 – -2.4 -3.1 -2.9 – 6.4 9.6 3.7 2.5 -0.6
Nucleus 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0
Vacuole -1.4 0.3 -1.4 -0.6 0.6 – -1.0 -3.1 -0.8 – 0 0 -1.9 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.515 3.5681 4.3332 4.7227 4.0837 4.3534 5.3976 5.7937 5.2968 5.3513 4.9447 5.7873 6.1946 6.3277 6.1961 5.6617 5.455 5.895
Actin 0.0952 0.0638 0.046 0.0497 0.1352 0.1016 0.1815 0.0725 0.0719 0.1544 0.3098 0.1132 0.2993 0.0789 0.0446 0.1374 0.01 0.1419
Bud 0.0662 0.0486 0.2888 0.169 0.0745 0.0146 0.0592 0.0849 0.2196 0.0638 0.0419 0.0073 0.0012 0.0429 0.1691 0.0619 0.0932 0.0158
Bud Neck 0.0486 0.0058 0.0054 0.0077 0.0436 0.5192 0.0743 0.0037 0.0273 0.0047 0.0103 0.5307 0.0042 0.0157 0.0453 0.0032 0.001 0.5851
Bud Periphery 0.1167 0.0644 0.4115 0.4194 0.2152 0.0408 0.1475 0.0773 0.4737 0.2082 0.2012 0.0203 0.0006 0.0464 0.4615 0.1981 0.3858 0.0334
Bud Site 0.1305 0.3287 0.0689 0.0194 0.0302 0.0281 0.0659 0.4592 0.0532 0.0223 0.0587 0.0129 0.3195 0.3604 0.0397 0.0188 0.0019 0.0302
Cell Periphery 0.0158 0.0086 0.0061 0.0027 0.0068 0.0083 0.0203 0.0029 0.0027 0.005 0.0079 0.0035 0.0071 0.0029 0.0031 0.0061 0.001 0.0067
Cytoplasm 0.1635 0.175 0.0327 0.0883 0.1484 0.0828 0.179 0.1562 0.0288 0.1908 0.0644 0.1378 0.14 0.2089 0.0837 0.1332 0.0122 0.0714
Cytoplasmic Foci 0.0747 0.0746 0.0185 0.0306 0.0375 0.0561 0.0765 0.0426 0.0292 0.1243 0.0685 0.0527 0.0496 0.0685 0.0262 0.0999 0.0866 0.0325
Eisosomes 0.001 0.0004 0.0002 0.0001 0.0004 0.0004 0.0018 0.0001 0.0009 0.0003 0.0012 0.0002 0.0008 0.0001 0.0001 0.0007 0 0.0001
Endoplasmic Reticulum 0.0233 0.0068 0.0037 0.0047 0.0148 0.0051 0.0181 0.0222 0.007 0.0038 0.0081 0.0061 0.0046 0.0245 0.0027 0.008 0.0007 0.0028
Endosome 0.0949 0.1292 0.0484 0.0826 0.0875 0.0619 0.0495 0.0362 0.0162 0.1148 0.0987 0.0446 0.0648 0.039 0.0533 0.1317 0.0906 0.0149
Golgi 0.0605 0.0552 0.028 0.032 0.084 0.044 0.051 0.0195 0.0106 0.0586 0.0761 0.0424 0.03 0.0822 0.0612 0.076 0.2908 0.0454
Lipid Particles 0.0194 0.0051 0.0041 0.0022 0.0254 0.0049 0.0126 0.0007 0.0042 0.0058 0.0092 0.0047 0.056 0.0057 0.0009 0.0324 0.0012 0.0009
Mitochondria 0.0198 0.0079 0.0096 0.0143 0.0325 0.0082 0.0128 0.0014 0.0214 0.0064 0.0136 0.0028 0.0003 0.003 0.0021 0.0416 0.0176 0.002
None 0.0061 0.0056 0.0047 0.0021 0.0217 0.0015 0.0099 0.0037 0.0193 0.0017 0.0171 0.0039 0.0022 0.0038 0.0004 0.0027 0.0005 0.0003
Nuclear Periphery 0.0075 0.0012 0.0015 0.0018 0.0082 0.0011 0.009 0.0016 0.0027 0.0011 0.0009 0.0004 0.0002 0.0028 0.0006 0.0013 0.0002 0.0002
Nucleolus 0.0011 0.0005 0.0006 0.0003 0.0013 0.0007 0.0004 0.0002 0.0002 0.0002 0.0003 0.0007 0.0002 0.0002 0.0001 0.0009 0 0.0007
Nucleus 0.0028 0.0015 0.0018 0.0019 0.0042 0.0024 0.0014 0.0011 0.0005 0.0009 0.0005 0.001 0.0003 0.0008 0.0004 0.001 0.0003 0.0022
Peroxisomes 0.0175 0.0036 0.0055 0.0105 0.0063 0.0056 0.0193 0.0018 0.0041 0.0121 0.0049 0.0053 0.0012 0.0077 0.0008 0.0205 0.0044 0.0034
Punctate Nuclear 0.0068 0.0012 0.0013 0.0008 0.0053 0.0009 0.0018 0.0004 0.0009 0.0014 0.0015 0.0007 0.0004 0.0005 0.0002 0.0073 0.0001 0.0003
Vacuole 0.0247 0.0106 0.0104 0.0511 0.0133 0.01 0.0069 0.0108 0.0046 0.0158 0.0037 0.008 0.0172 0.0046 0.003 0.0137 0.0012 0.0094
Vacuole Periphery 0.0033 0.0015 0.0024 0.0086 0.0038 0.002 0.0014 0.0009 0.0012 0.0037 0.0012 0.0008 0.0004 0.0006 0.001 0.0034 0.0007 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 75.5307 45.2928 33.6092 46.1305 62.346 46.4186 41.4492 37.4913 44.4437 75.498
Translational Efficiency 0.8289 0.905 0.8488 0.7244 0.8536 1.3639 0.8931 0.7832 0.7413 0.7823

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative calcium channel involved in calcium release under hypotonic stress; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Flc2

Flc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Flc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available