Standard name | |
---|---|
Human Ortholog | |
Description | Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | – | 3 | 2 | 5 | 12 | 18 | 8 | 10 | 9 |
Bud | 2 | 3 | 1 | 3 | 1 | 4 | 7 | 11 | – | 10 | 19 | 32 | 0 | 0 | 0 | – | 1 | 1 | 3 | 6 | 6 | 4 | 9 | 9 |
Bud Neck | 0 | 2 | 2 | 0 | 0 | 1 | 1 | 3 | – | 2 | 1 | 1 | 0 | 1 | 0 | – | 6 | 2 | 4 | 8 | 7 | 4 | 4 | 7 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | – | 4 | 0 | 2 | 0 | 0 | 0 | – | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 10 | 5 | 4 | 3 | 2 | 0 | 6 | 7 | – | 5 | 14 | 12 | 1 | 1 | 2 | – | 0 | 5 | 3 | 11 | 9 | 6 | 12 | 11 |
Cytoplasm | 274 | 559 | 390 | 287 | 332 | 329 | 342 | 406 | – | 268 | 338 | 360 | 188 | 188 | 186 | – | 161 | 117 | 192 | 441 | 187 | 94 | 195 | 180 |
Endoplasmic Reticulum | 7 | 28 | 20 | 4 | 0 | 7 | 3 | 2 | – | 1 | 9 | 5 | 3 | 3 | 5 | – | 17 | 5 | 61 | 67 | 119 | 36 | 44 | 50 |
Endosome | 0 | 0 | 4 | 0 | 11 | 1 | 2 | 3 | – | 0 | 0 | 1 | 0 | 0 | 0 | – | 4 | 3 | 6 | 3 | 5 | 2 | 2 | 2 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 5 |
Mitochondria | 21 | 12 | 17 | 52 | 44 | 225 | 251 | 290 | – | 337 | 471 | 442 | 0 | 0 | 0 | – | 19 | 9 | 41 | 43 | 80 | 38 | 45 | 30 |
Nucleus | 1 | 0 | 0 | 2 | 0 | 1 | 2 | 8 | – | 3 | 2 | 2 | 0 | 0 | 0 | – | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | – | 2 | 3 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 |
Nucleolus | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | – | 0 | 0 | 1 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 3 | 2 | 2 | 3 | 6 | 5 | – | 5 | 5 | 12 | 0 | 0 | 0 | – | 2 | 2 | 4 | 3 | 6 | 1 | 4 | 4 |
Vac/Vac Membrane | 10 | 5 | 7 | 7 | 18 | 8 | 16 | 12 | – | 15 | 14 | 13 | 1 | 2 | 1 | – | 2 | 3 | 10 | 15 | 11 | 5 | 10 | 15 |
Unique Cell Count | 301 | 596 | 433 | 321 | 370 | 467 | 496 | 629 | 490 | 685 | 693 | 192 | 192 | 192 | 181 | 133 | 340 | 630 | 469 | 208 | 345 | 335 | ||
Labelled Cell Count | 330 | 616 | 451 | 360 | 412 | 581 | 639 | 749 | 652 | 876 | 886 | 194 | 196 | 194 | 217 | 152 | 340 | 630 | 469 | 208 | 345 | 335 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 4.1 | 4.0 | 3.6 | 3.8 | 3.0 | 3.0 | 3.3 | – | 2.8 | 2.8 | 3.1 | 5.6 | 5.6 | 5.6 | 4.8 | 5.1 | 5.9 | 5.6 | 5.7 | 6.3 |
Std Deviation (1e-4) | 0.8 | 0.7 | 1.3 | 1.1 | 1.8 | 1.2 | 1.4 | 1.1 | – | 0.9 | 0.8 | 0.9 | 1.2 | 1.2 | 1.0 | 1.4 | 1.2 | 1.7 | 2.2 | 2.1 | 2.9 |
Intensity Change (Log2) | – | – | – | -0.17 | -0.1 | -0.45 | -0.41 | -0.31 | – | -0.54 | -0.51 | -0.41 | 0.47 | 0.47 | 0.46 | 0.25 | 0.33 | 0.54 | 0.48 | 0.5 | 0.63 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.8806 | 1.2084 | 1.0338 | 0.8952 | 1.1424 | 1.0188 | 1.3933 | 3.0669 | 2.9268 | 2.4407 | 2.344 | 2.4803 | 2.0563 | 2.583 | 2.242 | 2.7086 | 2.0899 | 2.6439 |
Actin | 0.0076 | 0.0008 | 0.0037 | 0.0047 | 0.0025 | 0.0009 | 0.015 | 0.0199 | 0.0129 | 0.0032 | 0.0052 | 0.0026 | 0.0044 | 0.0012 | 0.0018 | 0.0021 | 0.0151 | 0.0011 |
Bud | 0.0003 | 0.0036 | 0.0018 | 0.0015 | 0.0005 | 0.0004 | 0.0003 | 0.001 | 0.0017 | 0.0101 | 0.0003 | 0.0007 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Bud Neck | 0.002 | 0.0004 | 0.001 | 0.0004 | 0.0009 | 0.0068 | 0.0002 | 0.0128 | 0.0005 | 0.0046 | 0.0008 | 0.0017 | 0.0015 | 0.0002 | 0.002 | 0.0011 | 0.0002 | 0.0014 |
Bud Periphery | 0.0006 | 0.0035 | 0.0046 | 0.002 | 0.001 | 0.0009 | 0.0023 | 0.0024 | 0.014 | 0.0456 | 0.0007 | 0.0021 | 0.0007 | 0.0001 | 0.0004 | 0.0009 | 0.0002 | 0.0001 |
Bud Site | 0.0017 | 0.0095 | 0.0079 | 0.0043 | 0.001 | 0.0005 | 0.0005 | 0.0195 | 0.0087 | 0.0372 | 0.0017 | 0.0033 | 0.0007 | 0.0017 | 0.004 | 0.0013 | 0.0002 | 0.0002 |
Cell Periphery | 0.0003 | 0.0008 | 0.0013 | 0.0002 | 0.0003 | 0.0002 | 0.0006 | 0.0017 | 0.001 | 0.0024 | 0.0001 | 0.0003 | 0.001 | 0.0007 | 0.0008 | 0.0013 | 0.0003 | 0.0004 |
Cytoplasm | 0.1101 | 0.1619 | 0.1477 | 0.1763 | 0.1009 | 0.1424 | 0.1231 | 0.1936 | 0.1416 | 0.2018 | 0.1431 | 0.2348 | 0.2077 | 0.2201 | 0.2425 | 0.1559 | 0.2273 | 0.2691 |
Cytoplasmic Foci | 0.0175 | 0.0057 | 0.0192 | 0.0094 | 0.0045 | 0.0089 | 0.0042 | 0.0145 | 0.0093 | 0.0116 | 0.0347 | 0.0131 | 0.0073 | 0.0068 | 0.0101 | 0.0268 | 0.0052 | 0.0118 |
Eisosomes | 0.0003 | 0.0002 | 0.0006 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0007 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0075 | 0.0072 | 0.0154 | 0.0136 | 0.0097 | 0.0028 | 0.0107 | 0.0125 | 0.0087 | 0.0061 | 0.0007 | 0.0066 | 0.0326 | 0.0206 | 0.0133 | 0.0183 | 0.0257 | 0.0218 |
Endosome | 0.0078 | 0.0049 | 0.0055 | 0.0097 | 0.0099 | 0.0031 | 0.001 | 0.0045 | 0.0028 | 0.0143 | 0.004 | 0.0031 | 0.008 | 0.0031 | 0.0047 | 0.0056 | 0.0038 | 0.003 |
Golgi | 0.001 | 0.0003 | 0.0011 | 0.0018 | 0.0015 | 0.0004 | 0.0015 | 0.0016 | 0.001 | 0.0024 | 0.0068 | 0.0007 | 0.0012 | 0.0005 | 0.0005 | 0.0014 | 0.0045 | 0.0003 |
Lipid Particles | 0.0023 | 0.0008 | 0.0021 | 0.0017 | 0.0021 | 0.0015 | 0.0022 | 0.0016 | 0.0016 | 0.0045 | 0.017 | 0.0008 | 0.0047 | 0.0012 | 0.0022 | 0.002 | 0.0025 | 0.0008 |
Mitochondria | 0.0015 | 0.0006 | 0.0029 | 0.0042 | 0.0064 | 0.0011 | 0.0011 | 0.001 | 0.0195 | 0.0017 | 0.0189 | 0.0078 | 0.0006 | 0.0003 | 0.0008 | 0.0003 | 0.002 | 0.0005 |
None | 0.8105 | 0.778 | 0.7602 | 0.7537 | 0.8009 | 0.8186 | 0.826 | 0.7027 | 0.7668 | 0.6063 | 0.6972 | 0.7114 | 0.7188 | 0.7391 | 0.7007 | 0.7744 | 0.7073 | 0.6809 |
Nuclear Periphery | 0.006 | 0.0028 | 0.0043 | 0.0029 | 0.0053 | 0.0013 | 0.0015 | 0.0005 | 0.0015 | 0.0026 | 0.0002 | 0.0005 | 0.0022 | 0.0009 | 0.001 | 0.0009 | 0.0007 | 0.0015 |
Nucleolus | 0.0013 | 0.001 | 0.0013 | 0.0008 | 0.0018 | 0.0007 | 0.0001 | 0.0003 | 0.0003 | 0.0034 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0004 |
Nucleus | 0.0056 | 0.0086 | 0.0047 | 0.0037 | 0.0417 | 0.0017 | 0.0005 | 0.0006 | 0.0014 | 0.0052 | 0.0002 | 0.0005 | 0.0015 | 0.0011 | 0.0013 | 0.0007 | 0.0006 | 0.0018 |
Peroxisomes | 0.0028 | 0.0002 | 0.0039 | 0.0035 | 0.0008 | 0.0009 | 0.0074 | 0.0022 | 0.0036 | 0.0022 | 0.0665 | 0.0086 | 0.0031 | 0.0001 | 0.0101 | 0.0032 | 0.0027 | 0.001 |
Punctate Nuclear | 0.0095 | 0.0066 | 0.0084 | 0.0033 | 0.0024 | 0.0049 | 0.0004 | 0.0011 | 0.0011 | 0.0207 | 0.0009 | 0.0006 | 0.0011 | 0.0005 | 0.002 | 0.0026 | 0.0006 | 0.0028 |
Vacuole | 0.0029 | 0.0022 | 0.002 | 0.0018 | 0.0039 | 0.0013 | 0.001 | 0.005 | 0.0012 | 0.0126 | 0.0004 | 0.0005 | 0.002 | 0.0012 | 0.0012 | 0.0008 | 0.0008 | 0.0008 |
Vacuole Periphery | 0.0008 | 0.0003 | 0.0005 | 0.0005 | 0.0019 | 0.0004 | 0.0002 | 0.0003 | 0.0005 | 0.0014 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.7963 | 15.0342 | 21.827 | 15.9635 | 15.1688 | 19.7319 | 17.5143 | 21.6346 | 19.2099 | 22.0033 |
Translational Efficiency | 0.9319 | 0.6586 | 0.5146 | 0.6786 | 0.767 | 0.5286 | 0.5047 | 0.4943 | 0.5687 | 0.5515 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|