Standard name
Human Ortholog
Description Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.99 0.99 0.96 0.88 0.77 0.7 0.67 0.66 0.63 0.62 0.98 0.99 0.99 0.98 0.96 0.98 0.85 0.9 0.93 0.85 0.91 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0 0.14 0.16 0.1 0.13 0.1 0.17 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.06 0.13 0.23 0.21 0.23 0.22 0.26 0 0 0 0 0 0 0 0 0 0.08 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 2 1 4 3 8 0 0 0 0 1 0 0 0 3 0 1 0
Bud 1 1 1 2 4 2 21 22 24 22 17 24 0 1 0 0 0 0 3 4 3 1 6 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2 4 1 5 8 16 37 22 26 30 37 0 1 1 9 7 1 0 0 0 0 0 0
Cytoplasm 241 176 394 414 631 671 832 809 455 608 446 509 79 308 371 217 263 189 255 163 360 102 362 373
Endoplasmic Reticulum 0 0 1 0 1 2 3 13 11 20 5 19 1 2 1 7 4 5 8 0 2 0 0 0
Endosome 0 0 0 1 5 3 2 1 0 1 0 1 0 0 0 2 1 1 3 1 2 0 4 2
Golgi 0 0 0 0 0 0 0 1 0 0 1 1 0 0 0 2 1 0 0 0 2 0 0 0
Mitochondria 3 1 4 19 27 110 178 112 87 95 117 99 2 1 0 1 1 1 11 3 5 2 0 6
Nucleus 3 1 1 0 4 5 13 19 20 13 11 12 1 0 1 1 2 4 0 3 0 2 2 4
Nuclear Periphery 2 0 0 0 1 5 15 9 9 9 14 9 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 1 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 0 1 0 3 0 0 0 1 0 0 0 0 1 0 2 0 1 0 1 3
Vac/Vac Membrane 2 1 3 2 13 43 144 263 139 213 157 211 0 1 0 3 10 4 7 1 1 9 11 19
Unique Cell Count 247 177 400 420 656 762 1079 1158 677 925 704 822 81 312 373 222 274 193 300 181 388 120 398 422
Labelled Cell Count 252 183 408 441 691 850 1225 1292 768 1011 803 933 83 314 374 242 291 205 300 181 388 120 398 422


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 6.0 6.1 5.2 5.8 6.4 7.7 9.2 10.5 12.0 12.6 14.8 6.0 6.6 6.5 11.9 11.2 10.0 6.3 7.1 7.5
Std Deviation (1e-4) 1.3 3.1 2.1 1.5 2.1 2.8 3.5 4.5 5.8 6.8 8.3 9.3 1.6 2.0 2.4 7.3 3.7 2.8 1.7 3.0 5.6
Intensity Change (Log2) -0.22 -0.05 0.08 0.34 0.6 0.79 0.99 1.06 1.29 -0.02 0.12 0.11 0.97 0.88 0.72 0.06 0.24 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 2.4 2.4 3.5 2.7 2.7 3.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.7 2.4 2.3 2.0 3.0 3.2 0 0 0 0 0 0
Cytoplasm 0.1 -2.2 -6.1 -9.7 -11.8 -12.1 -12.8 -13.2 -13.7 -0.6 0.2 1.3 -0.7 -2.0 -0.5
Endoplasmic Reticulum 0 0 0 0 0 0 2.6 0 2.7 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.1 2.9 7.3 8.1 5.7 6.8 5.9 8.0 6.5 0 0 0 0 0 0
Nucleus 0 0 0 0 2.1 3.1 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 2.8 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.6 4.1 7.2 10.1 9.3 10.1 9.8 10.8 0 0 0 0 2.7 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.826 5.9066 5.2619 4.9191 4.7895 5.3527 1.3832 1.5372 1.5479 1.5059 0.2685 1.3805 1.753 2.508 2.0522 1.8258 2.3203 2.1439
Actin 0.0078 0.0005 0.0072 0.0005 0.0035 0.0041 0.0097 0.0012 0.0115 0.0056 0.0001 0.0222 0.015 0.0002 0.0021 0.04 0.0114 0.0161
Bud 0.0008 0.0003 0.0039 0.0001 0.0001 0.0002 0.001 0.0049 0.0003 0.0006 0 0.0002 0.0134 0.0011 0.0102 0.0007 0.0401 0.0004
Bud Neck 0.0008 0.0002 0.0003 0.0002 0.0006 0.001 0.0021 0.0001 0.0002 0.0034 0.0004 0.0004 0.001 0.0001 0.0008 0.0004 0.0007 0.0014
Bud Periphery 0.0008 0.0001 0.0047 0 0.0001 0.0003 0.001 0.0019 0.0002 0.0004 0 0.0002 0.0031 0.0004 0.0029 0.0006 0.0091 0.0004
Bud Site 0.0065 0.0014 0.0037 0.0001 0.0001 0.0002 0.0097 0.0013 0.0004 0.0034 0 0.0002 0.0041 0.0031 0.0045 0.0003 0.0014 0.0003
Cell Periphery 0.0004 0.0001 0.0014 0 0.0001 0.0001 0.0005 0.0001 0.0001 0.0001 0 0 0.0004 0.0001 0.0004 0.0001 0.0006 0.0001
Cytoplasm 0.6703 0.9514 0.854 0.8867 0.8979 0.9128 0.7498 0.8929 0.9021 0.7191 0.9811 0.932 0.7235 0.9121 0.8828 0.7893 0.658 0.8911
Cytoplasmic Foci 0.0353 0.0037 0.0156 0.0032 0.0045 0.009 0.0368 0.0045 0.0053 0.0347 0.0014 0.0033 0.0322 0.0037 0.0147 0.007 0.0205 0.0167
Eisosomes 0.0001 0 0.0005 0 0 0.0001 0.0003 0 0 0 0 0.0001 0.0001 0 0 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0032 0.0027 0.0042 0.0021 0.0025 0.0023 0.0107 0.0017 0.0047 0.002 0.0026 0.0047 0.0071 0.002 0.0034 0.0043 0.005 0.0025
Endosome 0.0242 0.0008 0.0103 0.001 0.002 0.0117 0.0232 0.0018 0.016 0.0423 0.0004 0.0121 0.0332 0.0009 0.0052 0.0031 0.0193 0.0049
Golgi 0.0224 0.0001 0.0029 0.0001 0.0002 0.0037 0.0063 0.0004 0.0024 0.0353 0 0.0074 0.006 0.0001 0.0009 0.0008 0.0025 0.0036
Lipid Particles 0.0096 0.0001 0.0005 0 0.0002 0.0006 0.0159 0.0003 0.0003 0.0111 0 0.0001 0.0053 0.0001 0.0016 0.0009 0.002 0.0018
Mitochondria 0.0119 0.0001 0.0028 0.0001 0.0001 0.0042 0.0081 0.0045 0.0008 0.0035 0 0.0025 0.0078 0.0001 0.0011 0.0006 0.012 0.0015
None 0.1826 0.0319 0.0705 0.1 0.0843 0.0374 0.042 0.0788 0.0455 0.1212 0.0078 0.0057 0.104 0.0707 0.0524 0.1405 0.0809 0.044
Nuclear Periphery 0.001 0.0008 0.0026 0.0011 0.0007 0.0009 0.0148 0.0008 0.005 0.0012 0.001 0.0028 0.0099 0.0008 0.0015 0.0024 0.0923 0.0019
Nucleolus 0.0011 0.0001 0.0001 0 0 0 0.0051 0.0002 0 0.0001 0 0 0.001 0.0001 0.0007 0.0001 0.0039 0.0001
Nucleus 0.002 0.004 0.0052 0.0035 0.002 0.0084 0.0391 0.0026 0.0026 0.0025 0.0045 0.0034 0.0134 0.0029 0.004 0.0041 0.0233 0.0074
Peroxisomes 0.0126 0.0001 0.0065 0 0.0001 0.0007 0.0074 0.0005 0.0002 0.0093 0 0.0001 0.0039 0 0.0043 0.0014 0.0011 0.0033
Punctate Nuclear 0.003 0.0006 0.0016 0.0005 0.0005 0.0008 0.0078 0.0005 0.0005 0.0016 0.0003 0.0002 0.0065 0.0007 0.0021 0.0016 0.0048 0.0016
Vacuole 0.0028 0.0009 0.0009 0.0004 0.0006 0.0012 0.0051 0.0007 0.0015 0.0018 0.0002 0.0017 0.0073 0.0007 0.0041 0.0014 0.0057 0.0006
Vacuole Periphery 0.0007 0.0001 0.0003 0.0001 0.0001 0.0004 0.0036 0.0001 0.0006 0.0006 0 0.0007 0.0017 0.0001 0.0004 0.0003 0.0053 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.5196 0.7866 3.5021 6.0475 7.4155 14.9502 6.7602 11.0041 15.8311 7.4848
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3
Localization
Cell Percentages cytoplasm (44%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Bdh2

Bdh2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bdh2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available