Standard name | |
---|---|
Human Ortholog | |
Description | Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 4 | 3 | 8 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 1 | 0 |
Bud | 1 | 1 | 1 | 2 | 4 | 2 | 21 | 22 | 24 | 22 | 17 | 24 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 4 | 3 | 1 | 6 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 4 | 1 | 5 | 8 | 16 | 37 | 22 | 26 | 30 | 37 | 0 | 1 | 1 | 9 | 7 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 241 | 176 | 394 | 414 | 631 | 671 | 832 | 809 | 455 | 608 | 446 | 509 | 79 | 308 | 371 | 217 | 263 | 189 | 255 | 163 | 360 | 102 | 362 | 373 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 1 | 2 | 3 | 13 | 11 | 20 | 5 | 19 | 1 | 2 | 1 | 7 | 4 | 5 | 8 | 0 | 2 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 1 | 5 | 3 | 2 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 1 | 3 | 1 | 2 | 0 | 4 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Mitochondria | 3 | 1 | 4 | 19 | 27 | 110 | 178 | 112 | 87 | 95 | 117 | 99 | 2 | 1 | 0 | 1 | 1 | 1 | 11 | 3 | 5 | 2 | 0 | 6 |
Nucleus | 3 | 1 | 1 | 0 | 4 | 5 | 13 | 19 | 20 | 13 | 11 | 12 | 1 | 0 | 1 | 1 | 2 | 4 | 0 | 3 | 0 | 2 | 2 | 4 |
Nuclear Periphery | 2 | 0 | 0 | 0 | 1 | 5 | 15 | 9 | 9 | 9 | 14 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 1 | 3 |
Vac/Vac Membrane | 2 | 1 | 3 | 2 | 13 | 43 | 144 | 263 | 139 | 213 | 157 | 211 | 0 | 1 | 0 | 3 | 10 | 4 | 7 | 1 | 1 | 9 | 11 | 19 |
Unique Cell Count | 247 | 177 | 400 | 420 | 656 | 762 | 1079 | 1158 | 677 | 925 | 704 | 822 | 81 | 312 | 373 | 222 | 274 | 193 | 300 | 181 | 388 | 120 | 398 | 422 |
Labelled Cell Count | 252 | 183 | 408 | 441 | 691 | 850 | 1225 | 1292 | 768 | 1011 | 803 | 933 | 83 | 314 | 374 | 242 | 291 | 205 | 300 | 181 | 388 | 120 | 398 | 422 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 6.0 | 6.1 | 5.2 | 5.8 | 6.4 | 7.7 | 9.2 | 10.5 | 12.0 | 12.6 | 14.8 | 6.0 | 6.6 | 6.5 | 11.9 | 11.2 | 10.0 | 6.3 | 7.1 | 7.5 |
Std Deviation (1e-4) | 1.3 | 3.1 | 2.1 | 1.5 | 2.1 | 2.8 | 3.5 | 4.5 | 5.8 | 6.8 | 8.3 | 9.3 | 1.6 | 2.0 | 2.4 | 7.3 | 3.7 | 2.8 | 1.7 | 3.0 | 5.6 |
Intensity Change (Log2) | – | – | – | -0.22 | -0.05 | 0.08 | 0.34 | 0.6 | 0.79 | 0.99 | 1.06 | 1.29 | -0.02 | 0.12 | 0.11 | 0.97 | 0.88 | 0.72 | 0.06 | 0.24 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 2.4 | 2.4 | 3.5 | 2.7 | 2.7 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0.7 | 2.4 | 2.3 | 2.0 | 3.0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.1 | -2.2 | -6.1 | -9.7 | -11.8 | -12.1 | -12.8 | -13.2 | -13.7 | -0.6 | 0.2 | 1.3 | -0.7 | -2.0 | -0.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 0 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.1 | 2.9 | 7.3 | 8.1 | 5.7 | 6.8 | 5.9 | 8.0 | 6.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 2.1 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.6 | 4.1 | 7.2 | 10.1 | 9.3 | 10.1 | 9.8 | 10.8 | 0 | 0 | 0 | 0 | 2.7 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.826 | 5.9066 | 5.2619 | 4.9191 | 4.7895 | 5.3527 | 1.3832 | 1.5372 | 1.5479 | 1.5059 | 0.2685 | 1.3805 | 1.753 | 2.508 | 2.0522 | 1.8258 | 2.3203 | 2.1439 |
Actin | 0.0078 | 0.0005 | 0.0072 | 0.0005 | 0.0035 | 0.0041 | 0.0097 | 0.0012 | 0.0115 | 0.0056 | 0.0001 | 0.0222 | 0.015 | 0.0002 | 0.0021 | 0.04 | 0.0114 | 0.0161 |
Bud | 0.0008 | 0.0003 | 0.0039 | 0.0001 | 0.0001 | 0.0002 | 0.001 | 0.0049 | 0.0003 | 0.0006 | 0 | 0.0002 | 0.0134 | 0.0011 | 0.0102 | 0.0007 | 0.0401 | 0.0004 |
Bud Neck | 0.0008 | 0.0002 | 0.0003 | 0.0002 | 0.0006 | 0.001 | 0.0021 | 0.0001 | 0.0002 | 0.0034 | 0.0004 | 0.0004 | 0.001 | 0.0001 | 0.0008 | 0.0004 | 0.0007 | 0.0014 |
Bud Periphery | 0.0008 | 0.0001 | 0.0047 | 0 | 0.0001 | 0.0003 | 0.001 | 0.0019 | 0.0002 | 0.0004 | 0 | 0.0002 | 0.0031 | 0.0004 | 0.0029 | 0.0006 | 0.0091 | 0.0004 |
Bud Site | 0.0065 | 0.0014 | 0.0037 | 0.0001 | 0.0001 | 0.0002 | 0.0097 | 0.0013 | 0.0004 | 0.0034 | 0 | 0.0002 | 0.0041 | 0.0031 | 0.0045 | 0.0003 | 0.0014 | 0.0003 |
Cell Periphery | 0.0004 | 0.0001 | 0.0014 | 0 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0006 | 0.0001 |
Cytoplasm | 0.6703 | 0.9514 | 0.854 | 0.8867 | 0.8979 | 0.9128 | 0.7498 | 0.8929 | 0.9021 | 0.7191 | 0.9811 | 0.932 | 0.7235 | 0.9121 | 0.8828 | 0.7893 | 0.658 | 0.8911 |
Cytoplasmic Foci | 0.0353 | 0.0037 | 0.0156 | 0.0032 | 0.0045 | 0.009 | 0.0368 | 0.0045 | 0.0053 | 0.0347 | 0.0014 | 0.0033 | 0.0322 | 0.0037 | 0.0147 | 0.007 | 0.0205 | 0.0167 |
Eisosomes | 0.0001 | 0 | 0.0005 | 0 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0032 | 0.0027 | 0.0042 | 0.0021 | 0.0025 | 0.0023 | 0.0107 | 0.0017 | 0.0047 | 0.002 | 0.0026 | 0.0047 | 0.0071 | 0.002 | 0.0034 | 0.0043 | 0.005 | 0.0025 |
Endosome | 0.0242 | 0.0008 | 0.0103 | 0.001 | 0.002 | 0.0117 | 0.0232 | 0.0018 | 0.016 | 0.0423 | 0.0004 | 0.0121 | 0.0332 | 0.0009 | 0.0052 | 0.0031 | 0.0193 | 0.0049 |
Golgi | 0.0224 | 0.0001 | 0.0029 | 0.0001 | 0.0002 | 0.0037 | 0.0063 | 0.0004 | 0.0024 | 0.0353 | 0 | 0.0074 | 0.006 | 0.0001 | 0.0009 | 0.0008 | 0.0025 | 0.0036 |
Lipid Particles | 0.0096 | 0.0001 | 0.0005 | 0 | 0.0002 | 0.0006 | 0.0159 | 0.0003 | 0.0003 | 0.0111 | 0 | 0.0001 | 0.0053 | 0.0001 | 0.0016 | 0.0009 | 0.002 | 0.0018 |
Mitochondria | 0.0119 | 0.0001 | 0.0028 | 0.0001 | 0.0001 | 0.0042 | 0.0081 | 0.0045 | 0.0008 | 0.0035 | 0 | 0.0025 | 0.0078 | 0.0001 | 0.0011 | 0.0006 | 0.012 | 0.0015 |
None | 0.1826 | 0.0319 | 0.0705 | 0.1 | 0.0843 | 0.0374 | 0.042 | 0.0788 | 0.0455 | 0.1212 | 0.0078 | 0.0057 | 0.104 | 0.0707 | 0.0524 | 0.1405 | 0.0809 | 0.044 |
Nuclear Periphery | 0.001 | 0.0008 | 0.0026 | 0.0011 | 0.0007 | 0.0009 | 0.0148 | 0.0008 | 0.005 | 0.0012 | 0.001 | 0.0028 | 0.0099 | 0.0008 | 0.0015 | 0.0024 | 0.0923 | 0.0019 |
Nucleolus | 0.0011 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0051 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.001 | 0.0001 | 0.0007 | 0.0001 | 0.0039 | 0.0001 |
Nucleus | 0.002 | 0.004 | 0.0052 | 0.0035 | 0.002 | 0.0084 | 0.0391 | 0.0026 | 0.0026 | 0.0025 | 0.0045 | 0.0034 | 0.0134 | 0.0029 | 0.004 | 0.0041 | 0.0233 | 0.0074 |
Peroxisomes | 0.0126 | 0.0001 | 0.0065 | 0 | 0.0001 | 0.0007 | 0.0074 | 0.0005 | 0.0002 | 0.0093 | 0 | 0.0001 | 0.0039 | 0 | 0.0043 | 0.0014 | 0.0011 | 0.0033 |
Punctate Nuclear | 0.003 | 0.0006 | 0.0016 | 0.0005 | 0.0005 | 0.0008 | 0.0078 | 0.0005 | 0.0005 | 0.0016 | 0.0003 | 0.0002 | 0.0065 | 0.0007 | 0.0021 | 0.0016 | 0.0048 | 0.0016 |
Vacuole | 0.0028 | 0.0009 | 0.0009 | 0.0004 | 0.0006 | 0.0012 | 0.0051 | 0.0007 | 0.0015 | 0.0018 | 0.0002 | 0.0017 | 0.0073 | 0.0007 | 0.0041 | 0.0014 | 0.0057 | 0.0006 |
Vacuole Periphery | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0036 | 0.0001 | 0.0006 | 0.0006 | 0 | 0.0007 | 0.0017 | 0.0001 | 0.0004 | 0.0003 | 0.0053 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 3.5196 | 0.7866 | 3.5021 | 6.0475 | 7.4155 | 14.9502 | 6.7602 | 11.0041 | 15.8311 | 7.4848 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|