Standard name
Human Ortholog
Description Member of the p24 family involved in ER to Golgi transport; role in misfolded protein quality control; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 – 0 0 0 0.06 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 – 0 – 0 0 0 0.06 – 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.44 0.57 0.52 0.77 – 0.78 – 0.78 0.8 0.77 0.81 – 0.28 0.23 0.44 0.1 0.09 0.12 0.05 0.05 0 0 0 0
Endoplasmic Reticulum 0.08 0.08 0.07 0.14 – 0.19 – 0.14 0.09 0.09 0.06 – 0.33 0.23 0.16 0 0 0 0.22 0.25 0.44 0.09 0.05 0.06
Endosome 0 0 0.06 0 – 0 – 0 0 0 0 – 0 0.05 0.06 0 0 0 0 0.09 0.07 0.09 0 0
Golgi 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0.07 0.05 0.08 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0.15 0.14 0.1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.65 0.4 0.41 0.18 – 0 – 0 0.09 0.14 0.12 – 0.42 0.54 0.44 0.8 0.84 0.83 0.64 0.56 0.42 0.71 0.8 0.83
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 2 – 5 – 13 9 10 14 – 0 5 2 0 0 0 0 0 1 0 0 0
Bud 0 2 0 2 – 9 – 22 15 15 24 – 0 0 0 1 0 0 1 1 0 1 2 3
Bud Neck 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 6 – 13 – 22 20 21 25 – 1 1 1 0 0 0 0 0 0 0 0 0
Cytoplasm 117 181 46 203 – 350 – 438 399 400 320 – 74 77 135 21 19 21 14 19 11 6 5 4
Endoplasmic Reticulum 20 25 6 36 – 85 – 78 44 46 22 – 87 78 49 5 8 5 62 90 163 22 10 17
Endosome 1 3 5 1 – 1 – 6 4 5 3 – 5 18 19 1 0 0 12 32 25 21 8 5
Golgi 9 9 1 1 – 1 – 1 0 1 0 – 8 8 2 14 12 14 2 2 5 2 2 2
Mitochondria 0 0 0 0 – 1 – 3 6 3 6 – 0 0 0 3 1 2 0 0 1 0 0 1
Nucleus 0 0 2 1 – 3 – 4 6 4 2 – 1 1 1 31 31 17 2 3 0 0 1 2
Nuclear Periphery 0 0 0 1 – 0 – 2 0 1 0 – 0 0 0 0 0 0 3 6 3 4 4 2
Nucleolus 1 4 1 0 – 0 – 2 0 1 0 – 1 3 1 4 0 1 1 0 0 2 0 0
Peroxisomes 0 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 – 0 0 1 0 – 0 2 0 1 0 0 0 1 0 0 1 0
Vac/Vac Membrane 173 129 36 48 – 13 – 25 46 72 47 – 109 182 136 168 185 145 178 206 154 171 165 221
Unique Cell Count 266 320 88 264 449 561 496 521 396 261 338 307 211 220 175 281 370 373 240 206 267
Labelled Cell Count 322 353 97 301 481 616 549 580 463 286 375 346 249 256 205 281 370 373 240 206 267


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 22.5 22.1 16.0 15.8 – 13.1 – 11.9 11.6 10.5 10.3 – 20.3 18.1 15.3 27.3 27.0 28.1 19.8 18.5 20.0
Std Deviation (1e-4) 5.2 4.5 3.5 3.3 – 3.0 – 2.6 2.6 2.6 2.3 – 4.9 4.2 3.9 11.5 10.0 9.6 5.0 4.8 4.8
Intensity Change (Log2) – – – -0.02 – -0.29 – -0.43 -0.47 -0.61 -0.64 – 0.34 0.18 -0.07 0.77 0.75 0.81 0.3 0.21 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Bud 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Cell Periphery 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 4.4 – 5.0 – 5.2 5.7 4.8 5.6 – -4.1 -5.4 -1.4 -8.0 -8.5 -7.1
Endoplasmic Reticulum 1.7 – 2.8 – 1.8 0.6 0.6 -0.5 – 4.9 3.4 2.2 0 0 0
Endosome 0 – 0 – 0 0 0 0 – 0 0 0.2 0 0 0
Golgi 0 – 0 – 0 0 0 0 – 0 0 0 0 0 2.3
Mitochondria 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Nucleus 0 – 0 – 0 0 0 0 – 0 0 0 3.1 3.0 2.2
Nuclear Periphery 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Vacuole -4.3 – -11.3 – -10.9 -7.9 -6.2 -6.5 – 0.1 2.2 0.6 6.6 7.6 6.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.4442 18.4211 19.0992 16.7348 15.2192 17.128 15.0583 23.3435 20.186 22.559 17.1298 21.0965 16.7881 23.332 22.9447 21.455 21.2185 21.4244
Actin 0.0033 0 0.0002 0 0.0534 0 0.0005 0 0.0095 0 0.0011 0.0035 0.0021 0 0 0 0.0001 0
Bud 0.0002 0.0002 0.0002 0.0001 0.0003 0.0002 0.0004 0.0002 0.0005 0.0001 0.0004 0.0001 0.0002 0.0001 0.0002 0 0.0001 0.0001
Bud Neck 0.0003 0.0003 0.0003 0.0001 0.0008 0.0001 0.0003 0.0001 0.0006 0.0002 0.0002 0.0001 0.0004 0.0001 0.0003 0 0.0001 0.0001
Bud Periphery 0.0005 0.0004 0.0005 0.0002 0.0008 0.0004 0.0006 0.0005 0.0011 0.0002 0.0008 0.0003 0.0004 0.0002 0.0004 0.0001 0.0003 0.0002
Bud Site 0.0008 0.0002 0.0003 0.0001 0.0005 0.0001 0.0004 0.0002 0.0005 0.0001 0.0005 0.0001 0.0013 0.0001 0.0002 0 0.0001 0
Cell Periphery 0.003 0.0041 0.0042 0.0006 0.0004 0.002 0.0019 0.0089 0.0024 0.001 0.0027 0.0003 0.0022 0.0063 0.0023 0.0005 0.0008 0.0018
Cytoplasm 0.0084 0.0192 0.0103 0.002 0.0012 0.0011 0.0117 0.0091 0.0193 0.0052 0.006 0.0009 0.0044 0.0096 0.0126 0.0022 0.0018 0.0009
Cytoplasmic Foci 0.0088 0.0002 0.0028 0.0108 0.0047 0.0004 0.0238 0.0002 0.0051 0.0017 0.0024 0.0006 0.0035 0.0002 0.0005 0.0002 0.011 0.0001
Eisosomes 0.0001 0 0 0 0.0006 0 0 0 0.0001 0 0.0002 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0113 0.0034 0.0081 0.0006 0.0032 0.001 0.0046 0.0098 0.009 0.0256 0.0218 0.004 0.0028 0.0035 0.0048 0.001 0.0028 0.0027
Endosome 0.0364 0.0106 0.0327 0.0175 0.0331 0.0276 0.0342 0.0097 0.0617 0.0454 0.0426 0.0288 0.0254 0.0067 0.0238 0.0165 0.0229 0.011
Golgi 0.0028 0.0001 0.0013 0.0013 0.0121 0.0007 0.002 0.0001 0.0158 0.0014 0.0013 0.0018 0.0007 0.0001 0.0003 0.0001 0.0006 0.0001
Lipid Particles 0.0063 0.0003 0.0021 0.0368 0.0186 0.0003 0.0035 0.0004 0.0025 0.0047 0.0047 0.001 0.002 0.0019 0.0005 0.0001 0.0075 0.0004
Mitochondria 0.0031 0.0002 0.0007 0.0003 0.0054 0.0006 0.0011 0.0004 0.0179 0.0004 0.0201 0.001 0.001 0.0001 0.0003 0 0.0003 0.0001
None 0.0079 0 0.0004 0.0001 0.0042 0 0.0472 0 0.0012 0.0001 0.0021 0.0002 0.0013 0 0 0 0.0012 0
Nuclear Periphery 0.0073 0.0112 0.0128 0.0025 0.0226 0.0178 0.0265 0.0192 0.0224 0.0388 0.0163 0.0153 0.0187 0.0088 0.0168 0.005 0.0282 0.0171
Nucleolus 0.006 0.0016 0.0005 0.0008 0.0008 0.0007 0.0006 0.0009 0.0005 0.0004 0.0008 0.0001 0.0025 0.0002 0.004 0.0001 0.0006 0.0003
Nucleus 0.0069 0.0048 0.0026 0.0012 0.0019 0.0034 0.0042 0.0039 0.0042 0.0039 0.0034 0.0008 0.0057 0.0014 0.0029 0.0008 0.0022 0.0018
Peroxisomes 0.0029 0 0.0001 0.0007 0.0047 0 0.001 0 0.0019 0 0.0006 0.0001 0.0004 0 0 0 0.0002 0
Punctate Nuclear 0.0035 0 0.0002 0.0022 0.0099 0 0.0087 0 0.0001 0.0005 0.0001 0.0001 0.0023 0 0 0 0.0041 0
Vacuole 0.5769 0.6524 0.5809 0.5767 0.462 0.4932 0.4837 0.5953 0.5586 0.5018 0.5006 0.3522 0.5338 0.6181 0.6227 0.5784 0.455 0.4392
Vacuole Periphery 0.3031 0.2907 0.339 0.3454 0.3588 0.4503 0.3432 0.3412 0.2653 0.3684 0.3713 0.5887 0.3887 0.3428 0.3075 0.395 0.4604 0.5241

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 228.2041 222.6819 165.2396 140.4358 167.9885 200.5079 194.5537 171.3413 164.2372 179.0983
Translational Efficiency 1.4831 1.3975 1.2726 1.82 1.4233 1.548 1.6761 1.3261 1.425 1.2848

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Member of the p24 family involved in ER to Golgi transport; role in misfolded protein quality control; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (28%), ER (14%), mixed (50%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Erp1

Erp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available