Standard name
Human Ortholog
Description FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier and is involved in gene tethering at the nuclear periphery; relocalizes to the cytosol in response to hypoxia; both NUP1 and NUP60 are homologous to human NUP153

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.19 0.1 0.17 0.1 0.26 0.25 0.32 0.42 0.45 0.5 0.43 0 0 0 0.14 0.1 0.09 0 0 0 0.05 0.09 0.12
Nucleus 0 0 0 0 0 0 0.05 0.07 0 0.05 0.07 0.06 0.05 0.05 0.06 0.05 0.1 0 0 0 0 0 0 0
Nuclear Periphery 0.81 0.94 0.86 0.81 0.73 0.65 0.66 0.52 0.59 0.51 0.42 0.5 0.84 0.79 0.68 0.81 0.65 0.65 0.91 0.89 0.93 0.84 0.75 0.68
Nucleolus 0.28 0.06 0.3 0.22 0.21 0.26 0.24 0.3 0.35 0.4 0.34 0.34 0.08 0.09 0.07 0.05 0.17 0.24 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.23 0.19 0.15 0.11 0.14 0.1 0.1 0.06 0.05 0 0 0.07 0.22 0.24 0.33 0.38 0.35 0.35 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 1 1 0 0 0 2 1 4 0 0 0 0 0 1 1 3 3
Bud 0 0 0 0 1 1 1 3 3 6 3 0 1 1 1 0 0 0 0 0 2 1 5 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 4 0 0 1 5 5 2 0 7 4 0 0 0
Cell Periphery 0 0 0 1 0 0 2 1 0 0 0 0 2 0 2 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 6 15 25 16 22 22 15 12 10 5 17 44 97 0 4 1 0 0 0 0 0 0
Endoplasmic Reticulum 1 0 2 5 6 0 3 3 1 0 1 1 2 1 1 0 2 1 0 0 0 0 1 2
Endosome 4 0 14 10 23 9 26 20 7 7 4 6 14 23 19 0 5 2 0 0 2 11 32 45
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 1 1 2 0 0 0 1 0 2 0 9 9
Mitochondria 13 3 34 72 48 101 128 212 157 263 169 142 11 6 2 3 10 3 1 0 6 19 63 88
Nucleus 5 0 7 19 18 14 24 50 16 27 24 21 55 54 76 1 10 1 2 1 0 2 6 5
Nuclear Periphery 121 15 287 352 362 253 337 351 220 300 144 167 878 924 832 17 68 22 144 37 323 316 514 488
Nucleolus 42 1 99 98 103 102 125 203 130 236 116 114 88 106 81 1 18 8 1 0 2 5 10 11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 1 0 0 4 0 0 0 0 0 0 2 6 10
SpindlePole 0 0 0 0 1 0 2 3 4 4 4 2 2 15 40 0 0 0 0 0 1 2 4 5
Vac/Vac Membrane 34 3 50 50 69 40 51 43 20 22 13 25 231 282 404 8 37 12 3 2 3 11 18 32
Unique Cell Count 149 16 332 436 493 387 511 669 373 591 341 334 1048 1164 1229 21 105 34 159 42 349 376 682 719
Labelled Cell Count 220 22 499 623 656 540 721 913 575 882 494 486 1304 1465 1570 30 154 50 159 42 349 376 682 719


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.8 7.9 9.6 9.3 9.1 7.4 7.3 7.1 7.0 6.4 6.4 6.7 10.1 10.0 10.2 7.6 8.0 10.5 11.2 11.8 12.4
Std Deviation (1e-4) 1.9 1.7 2.5 2.7 2.9 2.3 2.0 2.4 2.0 1.8 1.9 1.8 1.8 2.0 2.4 1.4 2.0 2.0 2.1 2.3 2.3
Intensity Change (Log2) -0.05 -0.08 -0.38 -0.4 -0.44 -0.46 -0.57 -0.58 -0.51 0.07 0.06 0.09 -0.33 -0.27 0.13 0.23 0.3 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 2.4 1.8 2.0 1.3 1.7 0.2 1.0 -0.3 -0.2 1.8 4.0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -1.5 0.3 -1.4 0.6 -1.0 -1.8 -3.0 -2.4 -1.8 -3.2 -2.3 -3.0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.5 -0.2 5.4 5.3 7.4 9.5 10.7 11.1 9.4 -8.2 -9.7 -10.9 0 -0.2 0
Nucleus 1.7 1.3 1.2 2.0 3.4 1.6 1.9 3.1 2.7 2.4 2.1 2.9 0 0 0
Nuclear Periphery -2.1 -4.5 -6.5 -6.6 -10.5 -8.1 -10.8 -12.0 -10.1 -1.2 -2.9 -6.7 -0.7 -5.0 -3.3
Nucleolus -2.3 -2.9 -1.0 -1.7 0.2 1.4 3.1 1.2 1.2 -9.9 -9.7 -11.8 -2.5 -2.6 -0.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 3.3 0 0 0
Vacuole -1.5 -0.4 -1.9 -2.2 -4.4 -4.3 -6.2 -5.0 -3.1 2.8 3.5 6.3 0 4.5 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.8784 7.5092 7.2485 7.431 6.4145 7.1294 6.8866 7.4808 6.7502 6.3083 6.4548 6.4033 5.9172 6.9973 6.4466 6.6676 5.6473 6.2754
Actin 0.0093 0.0001 0.0111 0.0002 0.0003 0.0001 0.0197 0.0001 0.0011 0.0012 0.0022 0.0002 0.0045 0.0001 0.0006 0.0003 0.0002 0.0062
Bud 0.0001 0 0.0001 0 0 0 0.0006 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0 0
Bud Neck 0.0002 0 0.0001 0 0 0 0.0009 0 0 0.0001 0.0001 0 0.0001 0 0 0 0 0.0001
Bud Periphery 0.0001 0.0001 0.0001 0 0.0001 0 0.0012 0 0.0001 0.0002 0.0003 0 0.0003 0 0.0001 0.0001 0.0001 0.0001
Bud Site 0.0005 0.0001 0.0005 0 0.0001 0 0.0053 0 0.0002 0 0.0001 0 0.0001 0 0.0001 0 0 0
Cell Periphery 0.0001 0 0 0 0.0001 0 0.0003 0 0.0001 0 0.0001 0 0.0001 0 0 0 0 0
Cytoplasm 0.0014 0.0001 0.0001 0 0.0001 0 0.0065 0.0001 0.0017 0.0002 0.0001 0.0002 0.0013 0 0.0007 0.0001 0.0001 0.0003
Cytoplasmic Foci 0.0004 0.0009 0.0001 0 0.0002 0 0.0031 0.0002 0.0041 0.003 0.0013 0.0048 0.0017 0 0.0004 0.0067 0.0125 0.0005
Eisosomes 0.0006 0 0.0001 0 0 0 0.0004 0 0 0 0.0001 0 0.0001 0 0 0 0 0.0001
Endoplasmic Reticulum 0.0009 0.0002 0.0002 0.0004 0.0006 0.0001 0.0046 0.0008 0.0012 0.0015 0.0004 0.0001 0.0028 0.0006 0.0008 0.001 0.0007 0.0007
Endosome 0.0009 0.0003 0.0001 0.0001 0.0008 0.0001 0.0071 0.0006 0.0026 0.0034 0.009 0.0015 0.0087 0.0002 0.0029 0.0044 0.002 0.0027
Golgi 0.0005 0.0003 0.0002 0.0001 0.0002 0 0.0021 0.0004 0.0016 0.0021 0.0183 0.0003 0.0014 0.0001 0.0003 0.0023 0.0015 0.0009
Lipid Particles 0.0005 0.0031 0.0012 0.0005 0.0029 0.0002 0.003 0.0071 0.0119 0.014 0.0519 0.006 0.0164 0.0005 0.0014 0.0782 0.0432 0.0013
Mitochondria 0.0008 0.0004 0.0004 0.0005 0.0014 0.0005 0.0051 0.0008 0.0018 0.0021 0.0433 0.0002 0.0032 0.0007 0.0016 0.0021 0.0024 0.0004
None 0.0084 0.0001 0.0001 0.0001 0.0002 0 0.0059 0.0001 0.0044 0.0005 0.0003 0.0003 0.0019 0.0003 0.001 0.0003 0.0004 0.0019
Nuclear Periphery 0.9411 0.9106 0.9196 0.9121 0.9419 0.9715 0.8726 0.9175 0.8942 0.8966 0.7911 0.9672 0.904 0.9535 0.9356 0.8497 0.8987 0.9667
Nucleolus 0.0003 0.0041 0.0003 0.0009 0.0117 0.0008 0.0011 0.0004 0.0016 0.001 0.0051 0.0002 0.002 0.0004 0.0007 0.0007 0.0012 0.0006
Nucleus 0.0134 0.0558 0.0401 0.0616 0.0171 0.016 0.008 0.0228 0.0132 0.0053 0.0398 0.0027 0.0089 0.0026 0.0056 0.0024 0.0022 0.0041
Peroxisomes 0.0001 0.0001 0.0001 0 0.0001 0 0.001 0 0.0007 0.0009 0.0011 0.001 0.0004 0 0.0001 0.0008 0.0012 0.0002
Punctate Nuclear 0.0005 0.0031 0.0005 0.0004 0.0027 0.0005 0.0029 0.0006 0.0186 0.0213 0.0017 0.0019 0.0063 0.0004 0.0012 0.0037 0.0066 0.0017
Vacuole 0.0003 0.0004 0.0002 0.0001 0.001 0.0002 0.0044 0.0006 0.0008 0.0006 0.0012 0.0001 0.0016 0.0004 0.0011 0.0005 0.0006 0.0003
Vacuole Periphery 0.0195 0.0203 0.0249 0.0228 0.0185 0.0098 0.0443 0.0478 0.0398 0.0458 0.0325 0.0134 0.034 0.0402 0.0455 0.0465 0.0263 0.0112

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.6285 44.4478 25.7532 41.2129 33.5576 23.1451 29.8582 34.2796 30.7988 36.5373
Translational Efficiency 1.6764 1.2761 2.168 1.2251 1.5952 2.014 1.4814 1.4656 1.6473 1.5745

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier and is involved in gene tethering at the nuclear periphery; relocalizes to the cytosol in response to hypoxia; both NUP1 and NUP60 are homologous to human NUP153
Localization
Cell Percentages nuclear periphery (94%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Nup60

Nup60


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nup60-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available