Standard name | |
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Human Ortholog | |
Description | Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; role in DNA catenation/decatenation pathway of chromosome disentangling; relocalizes to the cytosol in response to hypoxia |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 6 |
Bud | 1 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 6 | 2 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 5 | 0 |
Bud Neck | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 1 | 6 | 8 | 8 | 1 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2 | – | 4 | 13 | 7 | 5 | – | 2 | 3 | 2 | 5 | 5 | 6 | 13 | 11 | 18 | 33 | 0 | 0 | 0 | 0 | 0 | 2 |
Endoplasmic Reticulum | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 4 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 5 | 0 | – | 1 | 2 | 4 | 15 | – | 3 | 15 | 1 | 5 | 1 | 5 | 20 | 0 | 2 | 1 | 9 | 1 | 1 | 2 | 23 | 28 |
Golgi | 0 | 0 | – | 0 | 1 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 11 | 2 | 8 | 2 | 0 | 0 | 1 | 4 | 16 |
Mitochondria | 14 | 0 | – | 2 | 4 | 9 | 18 | – | 42 | 50 | 48 | 45 | 1 | 1 | 2 | 9 | 6 | 5 | 8 | 1 | 0 | 0 | 18 | 24 |
Nucleus | 133 | 34 | – | 121 | 185 | 173 | 206 | – | 79 | 112 | 91 | 84 | 64 | 98 | 90 | 106 | 165 | 179 | 120 | 48 | 36 | 10 | 75 | 73 |
Nuclear Periphery | 5 | 1 | – | 2 | 1 | 3 | 5 | – | 1 | 10 | 2 | 8 | 0 | 1 | 3 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 3 |
Nucleolus | 54 | 13 | – | 26 | 51 | 46 | 92 | – | 78 | 146 | 95 | 100 | 13 | 18 | 31 | 20 | 24 | 31 | 20 | 11 | 6 | 0 | 9 | 13 |
Peroxisomes | 1 | 0 | – | 0 | 4 | 1 | 5 | – | 8 | 14 | 6 | 18 | 3 | 7 | 12 | 1 | 1 | 6 | 11 | 1 | 0 | 0 | 11 | 33 |
SpindlePole | 4 | 0 | – | 4 | 4 | 3 | 3 | – | 7 | 24 | 8 | 20 | 4 | 9 | 18 | 1 | 0 | 4 | 9 | 3 | 0 | 6 | 12 | 13 |
Vac/Vac Membrane | 3 | 2 | – | 4 | 7 | 8 | 14 | – | 10 | 8 | 6 | 7 | 3 | 3 | 8 | 15 | 15 | 27 | 24 | 10 | 3 | 4 | 49 | 51 |
Unique Cell Count | 190 | 47 | 152 | 254 | 242 | 334 | 195 | 332 | 218 | 246 | 87 | 135 | 167 | 156 | 223 | 277 | 215 | 83 | 52 | 29 | 219 | 272 | ||
Labelled Cell Count | 220 | 52 | 164 | 272 | 254 | 365 | 231 | 394 | 269 | 303 | 95 | 152 | 199 | 179 | 237 | 302 | 215 | 83 | 52 | 29 | 219 | 272 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.7 | 16.9 | – | 15.1 | 18.5 | 16.9 | 17.1 | – | 15.4 | 15.8 | 14.0 | 15.1 | 16.9 | 19.4 | 18.8 | 34.7 | 33.9 | 31.7 | – | 12.3 | 12.9 |
Std Deviation (1e-4) | 3.9 | 5.6 | – | 5.3 | 5.2 | 4.8 | 6.2 | – | 4.5 | 5.6 | 4.5 | 6.0 | 8.1 | 7.5 | 9.0 | 14.6 | 12.1 | 14.3 | – | 2.6 | 3.7 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.3646 | 11.9249 | 9.9813 | 7.8792 | 8.0751 | 11.9546 | 9.9573 | 14.8758 | 13.4959 | 9.8477 | 12.3295 | 12.4138 | 7.1601 | 12.029 | 11.2905 | 10.5938 | 6.3462 | 9.0142 |
Actin | 0.0496 | 0.0088 | 0.0253 | 0.0043 | 0.0045 | 0.002 | 0.0053 | 0.0008 | 0.0017 | 0.0208 | 0.0056 | 0.003 | 0.0617 | 0.0045 | 0.0301 | 0.0147 | 0.0459 | 0.0421 |
Bud | 0.0011 | 0.0008 | 0.0005 | 0.0013 | 0.0002 | 0.0018 | 0.0007 | 0.0002 | 0.0004 | 0.0004 | 0.0005 | 0.0005 | 0.0006 | 0.0015 | 0.0012 | 0.0004 | 0.0014 | 0.0025 |
Bud Neck | 0.0008 | 0.0009 | 0.0007 | 0.0015 | 0.0009 | 0.0037 | 0.0004 | 0.0003 | 0.0005 | 0.0004 | 0.0008 | 0.001 | 0.0011 | 0.0007 | 0.0007 | 0.0024 | 0.0024 | 0.0181 |
Bud Periphery | 0.0024 | 0.0024 | 0.0013 | 0.0039 | 0.001 | 0.0058 | 0.0014 | 0.0009 | 0.001 | 0.0006 | 0.0007 | 0.0021 | 0.0009 | 0.0035 | 0.0016 | 0.0005 | 0.0031 | 0.0059 |
Bud Site | 0.0025 | 0.002 | 0.0009 | 0.0015 | 0.0004 | 0.0009 | 0.002 | 0.0004 | 0.0006 | 0.001 | 0.0013 | 0.0003 | 0.0066 | 0.0035 | 0.009 | 0.0011 | 0.0037 | 0.0022 |
Cell Periphery | 0.0006 | 0.0009 | 0.0004 | 0.0015 | 0.0006 | 0.001 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0006 | 0.0005 | 0.0005 | 0.0006 | 0.0001 | 0.0004 | 0.001 |
Cytoplasm | 0.0051 | 0.0005 | 0.0008 | 0.0034 | 0.0003 | 0.0006 | 0.0045 | 0.0001 | 0.0005 | 0.0007 | 0.001 | 0.0002 | 0.0052 | 0.0003 | 0.0008 | 0.0003 | 0.0007 | 0.0009 |
Cytoplasmic Foci | 0.0344 | 0.0061 | 0.0039 | 0.0278 | 0.0272 | 0.0013 | 0.0112 | 0.0004 | 0.0034 | 0.0738 | 0.004 | 0.0017 | 0.0415 | 0.0346 | 0.0165 | 0.0359 | 0.0531 | 0.0096 |
Eisosomes | 0.0005 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0005 | 0.0001 | 0.0005 | 0.0004 |
Endoplasmic Reticulum | 0.0034 | 0.0003 | 0.0006 | 0.001 | 0.0002 | 0.0002 | 0.0025 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.0034 | 0.0002 | 0.0004 | 0.0001 | 0.0007 | 0.0011 |
Endosome | 0.0162 | 0.003 | 0.0057 | 0.012 | 0.0027 | 0.0021 | 0.0095 | 0.0009 | 0.0009 | 0.0097 | 0.0018 | 0.0006 | 0.0118 | 0.0014 | 0.0111 | 0.0022 | 0.0322 | 0.0159 |
Golgi | 0.0095 | 0.0099 | 0.0059 | 0.0036 | 0.0082 | 0.0011 | 0.0019 | 0.0005 | 0.0007 | 0.0121 | 0.0016 | 0.0018 | 0.0112 | 0.0032 | 0.0228 | 0.0093 | 0.0556 | 0.0176 |
Lipid Particles | 0.0556 | 0.0219 | 0.026 | 0.0349 | 0.2876 | 0.0149 | 0.0065 | 0.0038 | 0.0098 | 0.0126 | 0.0049 | 0.0516 | 0.0802 | 0.0807 | 0.0374 | 0.0104 | 0.1164 | 0.1049 |
Mitochondria | 0.0398 | 0.0252 | 0.0275 | 0.1159 | 0.0795 | 0.01 | 0.008 | 0.0083 | 0.0251 | 0.0049 | 0.0051 | 0.031 | 0.0284 | 0.0303 | 0.0412 | 0.0067 | 0.0561 | 0.04 |
None | 0.0194 | 0.0004 | 0.0007 | 0.0026 | 0.0004 | 0.0005 | 0.0031 | 0.0002 | 0.0005 | 0.0005 | 0.0008 | 0.0004 | 0.0025 | 0.0005 | 0.0008 | 0.0003 | 0.001 | 0.0008 |
Nuclear Periphery | 0.0553 | 0.0609 | 0.0754 | 0.0561 | 0.0197 | 0.0355 | 0.0237 | 0.0182 | 0.0204 | 0.0767 | 0.0704 | 0.0293 | 0.0407 | 0.0184 | 0.0787 | 0.0572 | 0.0225 | 0.0656 |
Nucleolus | 0.1778 | 0.2622 | 0.2107 | 0.0987 | 0.0682 | 0.2495 | 0.1453 | 0.1749 | 0.1884 | 0.0671 | 0.095 | 0.2659 | 0.1587 | 0.2095 | 0.1783 | 0.1846 | 0.2587 | 0.1979 |
Nucleus | 0.3913 | 0.5299 | 0.5484 | 0.3807 | 0.4073 | 0.6445 | 0.7123 | 0.7497 | 0.6681 | 0.5908 | 0.5818 | 0.5708 | 0.4531 | 0.5503 | 0.4904 | 0.3844 | 0.1774 | 0.4167 |
Peroxisomes | 0.0379 | 0.0047 | 0.0035 | 0.006 | 0.0387 | 0.001 | 0.0019 | 0.0004 | 0.0008 | 0.007 | 0.0028 | 0.0017 | 0.0191 | 0.0062 | 0.006 | 0.0211 | 0.1146 | 0.0129 |
Punctate Nuclear | 0.079 | 0.0495 | 0.0554 | 0.2175 | 0.0369 | 0.0089 | 0.0562 | 0.0312 | 0.0687 | 0.1174 | 0.2193 | 0.019 | 0.0582 | 0.0426 | 0.06 | 0.2649 | 0.0484 | 0.0214 |
Vacuole | 0.0048 | 0.0027 | 0.0013 | 0.0107 | 0.003 | 0.0082 | 0.0015 | 0.003 | 0.0015 | 0.0006 | 0.0006 | 0.0026 | 0.0056 | 0.0018 | 0.002 | 0.0004 | 0.0024 | 0.0076 |
Vacuole Periphery | 0.0128 | 0.0068 | 0.0048 | 0.015 | 0.0121 | 0.0064 | 0.0019 | 0.005 | 0.0063 | 0.0021 | 0.0015 | 0.0155 | 0.0087 | 0.0055 | 0.0098 | 0.003 | 0.0028 | 0.015 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 111.7596 | 24.3158 | 24.7116 | 52.0624 | 67.4672 | 80.9661 | 28.9951 | 20.3814 | 39.3546 | 77.5565 |
Translational Efficiency | 1.8927 | 2.1745 | 1.3867 | 1.5583 | 2.1469 | 2.8681 | 1.5893 | 1.9465 | 1.7331 | 1.671 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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