Standard name
Human Ortholog
Description Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; forms a complex with Kel1p and Kel2p that regulates Bnr1p (formin) to affect actin cable assembly, cytokinesis, and polarized growth; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.73 0.71 0.59 0.41 0.35 0.62 0.72 0.73 0.71 0.69 0.63 0.71 0.39 0.29 0.37 0.47 0.52 0.5 0.52 0.52 0.53 0.55 0.19 0.16
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.06
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.07 0 0.21 0.23 0.3 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.06 0.05 0.08 0.18 0.26 0.13 0.08 0.1 0.05 0.05 0 0.06 0.06 0.15 0.09 0.09 0.09 0.15 0.3 0.31 0.27 0.12 0.24 0.22
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.33 0.28 0.43 0.56 0.49 0.29 0.19 0.18 0.16 0.15 0.17 0.14 0.6 0.65 0.59 0.63 0.56 0.58 0.05 0.08 0.13 0.18 0.29 0.27
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Bud 0 1 1 0 0 0 1 2 6 6 3 4 0 2 0 0 0 0 9 9 6 1 19 23
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 0 3 8
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 1 2 0 0 0 1 1 1 0 0 4 2 0 0 0 0 0 0 0
Cytoplasm 206 156 192 100 109 154 292 301 211 237 188 266 119 104 137 199 139 144 177 150 210 84 68 59
Endoplasmic Reticulum 3 5 8 3 3 5 5 8 2 0 1 5 5 3 2 14 4 9 1 0 1 8 25 22
Endosome 1 1 0 1 4 1 0 0 0 0 0 2 1 3 4 0 2 0 7 2 7 1 16 21
Golgi 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 8 2 3 0 0 2 0 0 3
Mitochondria 2 4 0 1 4 11 30 17 64 78 89 49 3 1 2 2 3 1 2 2 4 1 4 7
Nucleus 16 10 27 43 81 33 34 40 15 16 7 22 19 54 32 37 24 43 101 88 107 17 87 83
Nuclear Periphery 0 1 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 6 0 1 1 5 5
Nucleolus 0 0 0 0 0 0 2 0 0 0 0 0 1 1 1 0 0 0 1 0 0 0 5 14
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 4 2 2 1 7 9
Vac/Vac Membrane 92 61 138 136 151 72 79 76 48 52 50 52 182 235 219 266 149 168 18 23 51 27 103 101
Unique Cell Count 282 220 324 245 308 249 406 412 298 343 298 373 303 361 371 422 265 290 339 286 399 152 360 376
Labelled Cell Count 320 239 370 284 352 277 445 446 346 390 339 402 332 403 397 530 325 368 339 286 399 152 360 376


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.3 8.9 9.9 9.2 8.7 6.7 6.5 6.6 5.5 5.2 4.9 5.4 10.1 9.9 9.6 13.4 14.3 13.8 10.0 9.8 10.4
Std Deviation (1e-4) 1.8 2.1 1.3 1.4 1.3 1.1 1.1 1.1 1.1 1.0 1.0 0.9 1.6 1.7 1.7 2.7 2.7 2.4 1.6 1.8 1.9
Intensity Change (Log2) – – – -0.11 -0.19 -0.56 -0.61 -0.6 -0.85 -0.92 -1.03 -0.87 0.02 -0.01 -0.05 0.43 0.52 0.47 0.01 -0.01 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -4.4 -6.0 0.6 3.6 4.0 3.0 2.7 1.0 3.3 -5.0 -8.0 -5.9 -3.3 -1.7 -2.4
Endoplasmic Reticulum 0 -1.4 -0.4 -1.3 -0.5 0 0 0 -1.1 -0.7 -1.7 0 0.7 -0.8 0.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 5.0 3.7 8.8 9.1 0 6.8 0 0 0 0 0 0
Nucleus 3.3 6.0 1.9 0 0.6 -1.6 -1.9 -3.3 -1.3 -1.0 2.7 0.1 0.2 0.3 2.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.1 1.6 -3.4 -6.8 -7.2 -7.2 -7.8 -7.0 -8.5 4.4 5.9 4.3 5.6 3.3 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.8622 6.3968 5.5645 5.0263 4.5186 5.5152 5.4091 7.3396 6.5158 6.1828 4.9668 6.2215 4.8617 7.7004 6.1386 6.1478 5.6101 6.1079
Actin 0.0028 0.0001 0.0012 0.0003 0.0001 0.0001 0.0126 0.0003 0.0036 0.0006 0.0007 0.0002 0.0133 0.0002 0.0012 0.0245 0.0031 0.0036
Bud 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0009 0.0002 0.0004 0.0002 0.0003 0.0001 0.0006 0.0002 0.0003 0.0005 0.0002 0.0002
Bud Neck 0.0042 0.0005 0.0008 0.0012 0.001 0.0017 0.0036 0.0005 0.0008 0.0008 0.0011 0.0016 0.0029 0.0004 0.0013 0.0013 0.0008 0.002
Bud Periphery 0.0002 0 0 0 0 0 0.0008 0 0.0003 0.0002 0.0001 0 0.0006 0 0.0003 0.0003 0.0006 0.0001
Bud Site 0.0009 0.0004 0.0003 0.0001 0.0001 0.0001 0.0035 0.0004 0.002 0.0002 0.0004 0.0001 0.0037 0.0003 0.0009 0.0008 0.0002 0.0002
Cell Periphery 0.0001 0 0 0 0 0 0.0002 0 0 0.0001 0 0 0.0002 0 0.0001 0 0.0001 0
Cytoplasm 0.3598 0.4646 0.3409 0.4245 0.3779 0.3788 0.3907 0.5723 0.3766 0.3955 0.5116 0.3848 0.425 0.6515 0.4898 0.6017 0.4679 0.4942
Cytoplasmic Foci 0.0048 0.001 0.0031 0.0016 0.0011 0.0014 0.0176 0.0029 0.0017 0.0036 0.0131 0.0027 0.0144 0.0007 0.0077 0.0159 0.0025 0.0056
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0 0.0001 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0055 0.0024 0.0026 0.0019 0.0039 0.0021 0.0058 0.0022 0.0023 0.0064 0.0046 0.0034 0.0076 0.0012 0.0024 0.0028 0.0052 0.0031
Endosome 0.0097 0.0017 0.0084 0.0012 0.0039 0.0021 0.0317 0.0035 0.0035 0.0108 0.0326 0.0039 0.0302 0.0005 0.0093 0.009 0.0344 0.0082
Golgi 0.0008 0 0.0007 0 0 0 0.0059 0.0001 0.0004 0.0005 0.0145 0.0001 0.0102 0 0.0019 0.0037 0.0056 0.0043
Lipid Particles 0.0016 0 0.0005 0 0 0 0.0049 0 0.0002 0.001 0.0197 0 0.0171 0 0.0013 0.0007 0.0033 0.0026
Mitochondria 0.0022 0.0003 0.0005 0.0003 0.0004 0.0002 0.0074 0.0004 0.0018 0.0013 0.0075 0.0003 0.0064 0.0003 0.0011 0.0015 0.0022 0.0016
None 0.0096 0.0016 0.0042 0.0056 0.0052 0.0023 0.0025 0.0015 0.001 0.0044 0.0017 0.001 0.0066 0.0009 0.002 0.005 0.0011 0.002
Nuclear Periphery 0.0205 0.0155 0.0149 0.0139 0.0197 0.0147 0.0214 0.0115 0.015 0.0148 0.0191 0.0153 0.0196 0.0092 0.0133 0.0096 0.0233 0.0111
Nucleolus 0.0008 0.0004 0.0005 0.0004 0.0006 0.0004 0.001 0.0002 0.0004 0.0003 0.0003 0.0002 0.0011 0.0001 0.0004 0.0002 0.0003 0.0003
Nucleus 0.566 0.5072 0.6101 0.5378 0.5768 0.5908 0.4688 0.3989 0.5841 0.5504 0.3575 0.5804 0.4172 0.332 0.4546 0.3063 0.4387 0.45
Peroxisomes 0.0011 0 0.0002 0 0 0 0.0077 0.0001 0.0005 0.0001 0.0023 0.0001 0.0073 0 0.0028 0.0065 0.0002 0.0017
Punctate Nuclear 0.006 0.0022 0.0088 0.0103 0.0063 0.0031 0.0042 0.0034 0.0035 0.0027 0.0085 0.0043 0.012 0.0015 0.0056 0.0081 0.0052 0.0077
Vacuole 0.0026 0.0016 0.0017 0.0006 0.0025 0.0017 0.0069 0.0012 0.0017 0.0042 0.0034 0.0013 0.0028 0.0006 0.0032 0.0013 0.0027 0.0012
Vacuole Periphery 0.0005 0.0002 0.0003 0.0002 0.0005 0.0003 0.002 0.0003 0.0003 0.0019 0.0009 0.0003 0.0009 0.0001 0.0006 0.0003 0.0023 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.2261 22.2282 25.7723 31.1588 41.3064 33.1356 30.7859 30.0221 36.4563 37.6762
Translational Efficiency 1.3091 1.6866 1.2866 1.3126 1.0116 1.1543 0.8833 0.8433 0.8306 0.951

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; forms a complex with Kel1p and Kel2p that regulates Bnr1p (formin) to affect actin cable assembly, cytokinesis, and polarized growth; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages cytoplasm (88%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Bud14

Bud14


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bud14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available