Standard name
Human Ortholog
Description N-succinyl-5-aminoimidazole-4-carboxamide ribotide synthetase; required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 1.0 1.0 0.96 0.93 0.91 0.85 0.91 0.89 0.89 0.81 1.0 0.99 1.0 0.99 0.99 0.99 0.97 0.96 0.96 0.91 0.93 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0.08 0.1 0.05 0.1 0 0.05 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 4 2 6 8 0 0 0 0 0 0 0 0 0 1 0 0
Bud 2 0 0 0 0 1 1 1 0 0 1 0 0 2 0 0 1 0 1 4 1 11 14 11
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 1 4 3 4 9 4 9 10 5 0 3 3 2 1 3 0 0 0 3 1 0
Cytoplasm 421 685 229 205 234 255 260 357 290 279 151 122 408 508 531 293 266 199 398 649 206 387 419 441
Endoplasmic Reticulum 0 3 0 0 7 9 22 41 17 30 7 7 0 2 0 15 13 11 2 2 1 0 3 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0
Golgi 0 0 0 0 0 0 1 1 2 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 4 0 0 2 0 0 0 0 1 0 0 1 0 0 0 0
Nucleus 0 2 0 0 0 0 1 1 2 1 2 1 0 0 0 1 1 0 1 6 0 5 2 3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Peroxisomes 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 2 0 1 0 1 7 3 16 1 2 2 10 0 0 1 0 2 3 2 6 0 5 3 2
Unique Cell Count 422 690 230 205 243 273 287 418 318 314 170 150 408 511 532 295 268 202 412 679 214 427 453 470
Labelled Cell Count 425 691 231 206 246 275 293 426 324 323 180 156 408 516 535 312 285 216 412 679 214 427 453 470


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 109.3 98.7 90.9 76.7 54.8 50.4 46.9 46.2 40.5 39.2 35.7 34.2 122.0 113.9 106.3 81.9 104.9 115.5 89.4 94.0 99.6
Std Deviation (1e-4) 25.5 22.1 18.0 16.7 14.2 12.3 10.5 9.1 9.0 7.9 9.6 9.0 30.8 28.9 27.3 28.1 31.0 36.4 30.7 31.0 28.7
Intensity Change (Log2) – – – -0.24 -0.73 -0.85 -0.96 -0.98 -1.17 -1.21 -1.35 -1.41 0.43 0.33 0.23 -0.15 0.21 0.35 -0.02 0.05 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 -2.5 -3.6 -4.5 -5.9 -4.3 -5.0 -4.9 -6.5 1.3 -0.3 0.6 -0.4 -0.4 -1.1
Endoplasmic Reticulum 0 0 0 4.3 4.9 3.6 4.8 0 0 0 0 0 3.5 3.4 3.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.6 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 84.2425 101.1161 97.0251 92.4355 75.2124 92.9827 85.883 101.1004 99.2473 82.2166 75.6708 95.2382 93.7699 103.9921 101.3314 98.5306 91.5104 94.9721
Actin 0.0139 0.0001 0.0035 0.0003 0.0009 0.0002 0.0253 0.0012 0.0046 0.0471 0.0005 0.0038 0.0076 0.0001 0.0001 0 0.0003 0.0002
Bud 0.0002 0 0.0004 0.0001 0.0009 0.0003 0.0011 0.0013 0.0011 0.0005 0.0002 0.0003 0.0003 0 0 0 0.0001 0.0001
Bud Neck 0.0009 0.0001 0.0003 0.0001 0.0001 0.0004 0.0006 0.0002 0.0002 0.0003 0.0003 0.0002 0.004 0.0001 0.0001 0.0001 0.0002 0.0003
Bud Periphery 0.0002 0 0.0004 0.0001 0.0006 0.0001 0.0012 0.0004 0.0004 0.0003 0.0002 0.0001 0.0003 0 0 0 0 0
Bud Site 0.0005 0 0.0001 0.0004 0.0005 0 0.0017 0.0003 0.0002 0.0012 0.0003 0 0.0046 0 0 0 0.0003 0
Cell Periphery 0.0001 0 0.0002 0 0.0011 0 0.0107 0.0001 0.0001 0.0005 0.0011 0 0.0007 0 0 0 0.0001 0
Cytoplasm 0.8937 0.9964 0.9615 0.9486 0.7712 0.9771 0.8344 0.9871 0.9646 0.833 0.7631 0.9766 0.9298 0.9963 0.9917 0.9699 0.9173 0.9888
Cytoplasmic Foci 0.0164 0.0001 0.0019 0.0172 0.0212 0.0003 0.0075 0.0005 0.0021 0.0144 0.0191 0.0006 0.0043 0.0001 0 0 0.0283 0.0004
Eisosomes 0.0002 0 0 0.0001 0.0005 0 0.0012 0 0 0.0011 0 0 0.0002 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0005 0.0001 0.0006 0.0002 0.0014 0.0025 0.0056 0.0002 0.0002 0.0009 0.0098 0.0003 0.0003 0 0 0 0.0007 0.0001
Endosome 0.0013 0 0.0014 0.0005 0.0024 0.001 0.0139 0.0001 0.0018 0.0014 0.0043 0.0003 0.0017 0 0 0 0.0023 0.0001
Golgi 0.0013 0 0.0004 0.0003 0.0006 0 0.0027 0 0.0032 0.0043 0.0005 0.0003 0.0005 0 0 0 0.0006 0
Lipid Particles 0.0132 0 0.0016 0.0089 0.0192 0 0.0036 0 0.0016 0.0062 0.0012 0 0.0009 0 0 0 0.0087 0
Mitochondria 0.0027 0 0.0014 0.0023 0.034 0.0005 0.0038 0.0001 0.0057 0.048 0.0006 0.0006 0.0006 0 0 0.0001 0.0013 0.0001
None 0.0053 0.0001 0.0029 0.0029 0.0254 0.0001 0.0092 0.0004 0.0009 0.0185 0.0049 0.0003 0.0018 0.0001 0.0001 0.0001 0.0056 0.0002
Nuclear Periphery 0.0066 0.0006 0.0041 0.0015 0.0149 0.005 0.0222 0.0014 0.0009 0.0026 0.014 0.0021 0.0036 0.0004 0.0007 0.0013 0.0046 0.0015
Nucleolus 0.003 0 0.0019 0.0013 0.0309 0 0.0028 0.0001 0.0008 0.004 0.0068 0 0.0024 0 0 0 0.0026 0
Nucleus 0.0274 0.0024 0.0108 0.0102 0.0327 0.0075 0.0338 0.0055 0.0067 0.0075 0.0299 0.0137 0.0273 0.0028 0.0071 0.0283 0.0151 0.0079
Peroxisomes 0.0087 0 0.0006 0.0015 0.0018 0 0.0007 0 0.0025 0.0039 0.0002 0 0.0007 0 0 0 0.0006 0
Punctate Nuclear 0.0026 0 0.0025 0.0027 0.0055 0 0.0134 0.0003 0.0001 0.0011 0.0825 0.0003 0.0038 0 0 0 0.0098 0.0001
Vacuole 0.0008 0.0001 0.0017 0.0004 0.0108 0.0034 0.003 0.0006 0.0011 0.0014 0.0545 0.0002 0.0035 0 0 0.0001 0.0008 0.0002
Vacuole Periphery 0.0006 0 0.0018 0.0003 0.0233 0.0016 0.0016 0.0001 0.001 0.0015 0.006 0.0001 0.0013 0 0 0 0.0007 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 145.5631 66.401 72.3192 52.3641 59.803 120.9361 72.0109 66.5087 70.3878 71.465
Translational Efficiency 1.8207 1.9539 1.3867 1.7761 1.5801 1.9639 1.7965 1.7787 1.4785 1.7419

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description N-succinyl-5-aminoimidazole-4-carboxamide ribotide synthetase; required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Ade1

Ade1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ade1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available