Standard name | |
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Human Ortholog | |
Description | Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 0 | 0 | 2 | 1 | 1 | 2 | – | 2 | 1 | 2 | 2 | 5 | 4 | 2 | 2 | 1 | 0 | 0 | 1 | 1 | 2 | 6 |
Bud | – | 0 | 0 | 0 | 1 | 6 | 12 | 17 | – | 9 | 17 | 19 | 1 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 11 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 3 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 3 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 0 | 0 | 1 | 1 | 3 | 2 | – | 1 | 4 | 0 | 4 | 6 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | – | 20 | 96 | 144 | 281 | 273 | 423 | 422 | – | 353 | 249 | 396 | 172 | 267 | 349 | 209 | 171 | 175 | 0 | 25 | 44 | 36 | 129 | 134 |
Endoplasmic Reticulum | – | 0 | 1 | 0 | 6 | 17 | 14 | 10 | – | 2 | 6 | 23 | 6 | 23 | 39 | 6 | 8 | 6 | 4 | 1 | 8 | 2 | 5 | 28 |
Endosome | – | 0 | 0 | 3 | 2 | 0 | 18 | 12 | – | 6 | 6 | 1 | 0 | 0 | 2 | 18 | 13 | 3 | 2 | 2 | 5 | 0 | 4 | 7 |
Golgi | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 3 |
Mitochondria | – | 1 | 17 | 6 | 14 | 19 | 54 | 31 | – | 32 | 47 | 64 | 0 | 0 | 0 | 10 | 7 | 1 | 1 | 3 | 11 | 3 | 11 | 32 |
Nucleus | – | 0 | 0 | 0 | 0 | 2 | 0 | 2 | – | 4 | 2 | 2 | 1 | 0 | 0 | 14 | 3 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | 1 | 0 | 2 | 0 | 0 | 0 | 1 | – | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 1 | 3 | – | 1 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 |
Vac/Vac Membrane | – | 0 | 5 | 7 | 9 | 15 | 33 | 30 | – | 37 | 15 | 19 | 2 | 1 | 5 | 127 | 116 | 87 | 7 | 26 | 43 | 28 | 109 | 151 |
Unique Cell Count | 21 | 107 | 154 | 301 | 308 | 493 | 490 | 407 | 298 | 475 | 182 | 293 | 397 | 326 | 263 | 246 | 20 | 63 | 120 | 79 | 276 | 390 | ||
Labelled Cell Count | 22 | 119 | 162 | 316 | 334 | 560 | 536 | 448 | 354 | 527 | 191 | 310 | 406 | 390 | 322 | 281 | 20 | 63 | 120 | 79 | 276 | 390 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 4.1 | 3.8 | 3.9 | 4.6 | 4.7 | 4.4 | 5.2 | – | 5.1 | 5.1 | 5.1 | 5.1 | 5.0 | 5.7 | 7.5 | 7.3 | 7.6 | 5.4 | 5.7 | 6.2 |
Std Deviation (1e-4) | – | 0.8 | 0.8 | 1.0 | 1.8 | 1.5 | 1.6 | 4.8 | – | 1.7 | 1.8 | 1.7 | 1.0 | 1.0 | 1.2 | 1.8 | 1.7 | 1.9 | 1.4 | 1.6 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.26 | 0.3 | 0.21 | 0.45 | – | 0.42 | 0.42 | 0.43 | 0.42 | 0.4 | 0.59 | 0.98 | 0.94 | 1.0 | 0.5 | 0.58 | 0.69 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 6.7 | 7.3 | 6.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.5754 | 1.0485 | 0.6735 | 0.7753 | 0.0761 | 0.9361 | 0.7008 | 1.6605 | 1.4076 | 1.3819 | 0.6705 | 1.4166 | -0.9349 | -0.3722 | -0.6018 | -0.8176 | -0.5916 | -0.7984 |
Actin | 0.0295 | 0.0006 | 0.0102 | 0.0371 | 0.0153 | 0.0037 | 0.0198 | 0.0002 | 0.0045 | 0.0295 | 0.0002 | 0.0011 | 0.019 | 0.0005 | 0.0139 | 0.0001 | 0.0001 | 0.0002 |
Bud | 0.0009 | 0.001 | 0.0046 | 0.0007 | 0.0004 | 0.0011 | 0.0007 | 0.0009 | 0.0009 | 0.0004 | 0.0005 | 0.0008 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 |
Bud Neck | 0.0023 | 0.0003 | 0.0032 | 0.0008 | 0.0005 | 0.0022 | 0.0005 | 0.0003 | 0.0011 | 0.0003 | 0.0005 | 0.0013 | 0.0006 | 0.0001 | 0.0006 | 0.0005 | 0.0002 | 0.0005 |
Bud Periphery | 0.005 | 0.0011 | 0.0034 | 0.001 | 0.0007 | 0.0041 | 0.0022 | 0.0016 | 0.0016 | 0.0009 | 0.001 | 0.0011 | 0.0004 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0.0001 |
Bud Site | 0.0023 | 0.0065 | 0.0119 | 0.0021 | 0.0007 | 0.0006 | 0.0016 | 0.005 | 0.0138 | 0.0007 | 0.0005 | 0.0004 | 0.0018 | 0.0008 | 0.0032 | 0.0003 | 0.0002 | 0.0001 |
Cell Periphery | 0.0005 | 0.0004 | 0.0006 | 0.0002 | 0.0001 | 0.0003 | 0.0008 | 0.0011 | 0.0009 | 0.0004 | 0.0003 | 0.0003 | 0.0004 | 0.0003 | 0.0009 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.1565 | 0.2846 | 0.2249 | 0.1749 | 0.2451 | 0.3462 | 0.1918 | 0.3203 | 0.2163 | 0.2033 | 0.1516 | 0.309 | 0.2479 | 0.3249 | 0.2929 | 0.3172 | 0.2696 | 0.3606 |
Cytoplasmic Foci | 0.0566 | 0.0534 | 0.0532 | 0.0558 | 0.0548 | 0.068 | 0.0502 | 0.0443 | 0.0494 | 0.0613 | 0.0979 | 0.0549 | 0.0431 | 0.0501 | 0.0501 | 0.067 | 0.0598 | 0.0414 |
Eisosomes | 0.0005 | 0.0001 | 0.0003 | 0.0003 | 0.0005 | 0.0001 | 0.0007 | 0.0002 | 0.0007 | 0.0002 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0005 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0552 | 0.0337 | 0.035 | 0.0377 | 0.0142 | 0.0255 | 0.0395 | 0.0354 | 0.0573 | 0.0225 | 0.0126 | 0.0464 | 0.0944 | 0.0487 | 0.064 | 0.0295 | 0.0266 | 0.0422 |
Endosome | 0.1285 | 0.1557 | 0.1224 | 0.2459 | 0.1335 | 0.1246 | 0.1255 | 0.1063 | 0.1588 | 0.1771 | 0.3247 | 0.1664 | 0.1327 | 0.1278 | 0.128 | 0.2725 | 0.3609 | 0.1399 |
Golgi | 0.0112 | 0.005 | 0.0083 | 0.0174 | 0.0081 | 0.008 | 0.0069 | 0.0028 | 0.0066 | 0.0039 | 0.0047 | 0.0032 | 0.006 | 0.003 | 0.0051 | 0.0042 | 0.0054 | 0.0041 |
Lipid Particles | 0.0142 | 0.006 | 0.0104 | 0.0176 | 0.0235 | 0.0137 | 0.0155 | 0.0056 | 0.0075 | 0.0091 | 0.0135 | 0.0056 | 0.0216 | 0.0063 | 0.0194 | 0.0051 | 0.0046 | 0.004 |
Mitochondria | 0.0056 | 0.0048 | 0.0066 | 0.0053 | 0.0317 | 0.0111 | 0.0112 | 0.0039 | 0.0054 | 0.0028 | 0.0053 | 0.0047 | 0.0065 | 0.0008 | 0.0031 | 0.0035 | 0.0008 | 0.0037 |
None | 0.4662 | 0.3655 | 0.4478 | 0.3129 | 0.4042 | 0.3435 | 0.4573 | 0.4122 | 0.4125 | 0.4419 | 0.2484 | 0.3275 | 0.3596 | 0.3868 | 0.3628 | 0.1932 | 0.1689 | 0.3231 |
Nuclear Periphery | 0.0162 | 0.0093 | 0.0079 | 0.0143 | 0.01 | 0.0052 | 0.0092 | 0.0064 | 0.009 | 0.0047 | 0.009 | 0.005 | 0.0175 | 0.0103 | 0.0112 | 0.0119 | 0.0155 | 0.0066 |
Nucleolus | 0.0008 | 0.0006 | 0.0008 | 0.0005 | 0.0006 | 0.0007 | 0.0009 | 0.0009 | 0.0014 | 0.0005 | 0.001 | 0.0006 | 0.0006 | 0.0003 | 0.0007 | 0.0003 | 0.0002 | 0.0002 |
Nucleus | 0.0052 | 0.0041 | 0.0073 | 0.0033 | 0.0034 | 0.0029 | 0.0027 | 0.0036 | 0.006 | 0.0028 | 0.0036 | 0.0027 | 0.0031 | 0.0021 | 0.0066 | 0.004 | 0.0044 | 0.0029 |
Peroxisomes | 0.0121 | 0.0006 | 0.008 | 0.009 | 0.0079 | 0.0039 | 0.0064 | 0.0006 | 0.0048 | 0.0023 | 0.0015 | 0.0007 | 0.0054 | 0.0007 | 0.0109 | 0.0004 | 0.0003 | 0.0003 |
Punctate Nuclear | 0.005 | 0.0021 | 0.0031 | 0.0021 | 0.0118 | 0.0057 | 0.0038 | 0.001 | 0.0019 | 0.0017 | 0.0016 | 0.0016 | 0.004 | 0.0013 | 0.0032 | 0.0016 | 0.0009 | 0.0008 |
Vacuole | 0.0221 | 0.0585 | 0.025 | 0.0497 | 0.0269 | 0.0247 | 0.0471 | 0.0416 | 0.0349 | 0.0301 | 0.1075 | 0.0593 | 0.03 | 0.0322 | 0.0179 | 0.0768 | 0.0742 | 0.0629 |
Vacuole Periphery | 0.0037 | 0.0059 | 0.0051 | 0.0115 | 0.0061 | 0.0045 | 0.0057 | 0.0058 | 0.0047 | 0.0036 | 0.0141 | 0.0074 | 0.0048 | 0.0027 | 0.004 | 0.0115 | 0.0073 | 0.0062 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 24.1565 | 12.5387 | 20.6761 | 39.0644 | 29.2993 | 20.249 | 29.2295 | 35.1775 | 47.3948 | 39.4397 |
Translational Efficiency | 1.5845 | 2.1379 | 1.6231 | 1.2066 | 1.2885 | 2.9185 | 1.3043 | 1.1982 | 1.2037 | 1.3296 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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