Standard name | |
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Human Ortholog | |
Description | Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats and FFAT motif; interacts with ER anchor Scs2p at the nucleus-vacuole junction; regulated by sterol binding; SWH1 has a paralog, OSH2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 11 | 3 | 26 | 12 | 18 | 4 | 9 | 14 | 5 | – | 10 | 17 | 10 | 6 | 5 | 14 | 12 | 14 | 7 | 2 | 3 | 15 | 44 | 50 |
Bud | 3 | 2 | 3 | 1 | 3 | 5 | 12 | 13 | 13 | – | 12 | 24 | 10 | 9 | 3 | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 3 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | – | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 1 | 5 | 9 | 9 | 11 | 14 | 16 | 9 | – | 11 | 13 | 11 | 9 | 17 | 14 | 8 | 8 | 0 | 0 | 0 | 1 | 0 | 1 |
Endoplasmic Reticulum | 5 | 13 | 15 | 6 | 4 | 9 | 8 | 6 | 5 | – | 8 | 9 | 9 | 6 | 4 | 6 | 4 | 4 | 0 | 0 | 3 | 2 | 6 | 7 |
Endosome | 50 | 3 | 82 | 107 | 141 | 110 | 144 | 136 | 23 | – | 18 | 37 | 114 | 144 | 179 | 15 | 7 | 21 | 76 | 57 | 127 | 90 | 121 | 126 |
Golgi | 5 | 6 | 38 | 12 | 21 | 3 | 4 | 13 | 3 | – | 1 | 11 | 31 | 66 | 89 | 80 | 65 | 70 | 90 | 58 | 56 | 71 | 144 | 195 |
Mitochondria | 206 | 108 | 99 | 52 | 58 | 154 | 225 | 197 | 194 | – | 212 | 205 | 286 | 230 | 165 | 15 | 7 | 12 | 14 | 7 | 18 | 12 | 12 | 16 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 4 | 2 | – | 3 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 13 | 9 | 2 | 2 | 4 | 6 | 7 | 9 | 6 | – | 10 | 4 | 10 | 7 | 8 | 1 | 0 | 3 | 1 | 1 | 1 | 1 | 0 | 0 |
Nucleolus | 30 | 11 | 4 | 15 | 21 | 12 | 27 | 23 | 17 | – | 30 | 14 | 16 | 16 | 15 | 5 | 3 | 3 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 16 | 3 | 15 | 15 | 27 | 30 | 31 | 21 | 8 | – | 6 | 17 | 94 | 89 | 102 | 1 | 0 | 1 | 64 | 17 | 52 | 56 | 106 | 107 |
SpindlePole | 5 | 2 | 23 | 44 | 66 | 31 | 62 | 59 | 13 | – | 18 | 25 | 19 | 16 | 38 | 6 | 2 | 5 | 1 | 0 | 3 | 3 | 5 | 5 |
Vac/Vac Membrane | 8 | 5 | 15 | 7 | 8 | 16 | 16 | 34 | 11 | – | 5 | 17 | 12 | 24 | 33 | 11 | 8 | 15 | 1 | 4 | 3 | 1 | 1 | 0 |
Unique Cell Count | 256 | 124 | 232 | 202 | 274 | 294 | 423 | 423 | 246 | 265 | 293 | 440 | 442 | 485 | 129 | 94 | 119 | 263 | 153 | 274 | 262 | 451 | 521 | |
Labelled Cell Count | 359 | 168 | 328 | 282 | 381 | 393 | 562 | 545 | 310 | 347 | 396 | 623 | 624 | 658 | 169 | 120 | 156 | 263 | 153 | 274 | 262 | 451 | 521 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.4 | 8.4 | 8.2 | 7.7 | 7.9 | 6.6 | 6.7 | 7.0 | 6.0 | – | 5.5 | 6.3 | 9.0 | 9.2 | 10.3 | 15.4 | 16.1 | 15.0 | 8.4 | 8.2 | 8.8 |
Std Deviation (1e-4) | 1.1 | 1.8 | 1.2 | 1.0 | 1.1 | 1.2 | 1.2 | 1.2 | 1.1 | – | 1.1 | 1.1 | 1.2 | 2.2 | 3.6 | 3.2 | 3.3 | 3.0 | 2.4 | 1.2 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.05 | -0.32 | -0.3 | -0.24 | -0.46 | – | -0.58 | -0.39 | 0.13 | 0.16 | 0.32 | 0.91 | 0.97 | 0.87 | 0.03 | 0.0 | 0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.9 | -1.8 | -4.8 | -4.9 | -4.0 | -4.1 | – | -3.2 | -2.2 | -4.9 | -5.7 | -6.3 | -0.1 | 0.4 | 0.2 |
Bud | 0 | 0 | 0 | 1.3 | 1.4 | 2.4 | – | 2.1 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.4 | 0.8 | 1.1 | 0.8 | 1.1 | 1.0 | – | 1.3 | 1.4 | 0.3 | 0 | 1.0 | 3.5 | 0 | 0 |
Endoplasmic Reticulum | -1.7 | -3.0 | -1.9 | -3.0 | -3.5 | -2.4 | – | -1.8 | -1.8 | -2.9 | -3.6 | -4.4 | -0.7 | -0.8 | -1.2 |
Endosome | 3.7 | 3.6 | 0.5 | -0.3 | -0.8 | -6.9 | – | -7.9 | -6.2 | -2.6 | -0.7 | 0.4 | -4.9 | -5.1 | -3.4 |
Golgi | -3.4 | -3.0 | -6.5 | -7.7 | -6.1 | -5.9 | – | -6.6 | -4.9 | -3.8 | -0.5 | 0.6 | 8.9 | 9.3 | 8.2 |
Mitochondria | -3.7 | -5.2 | 2.2 | 2.6 | 1.0 | 8.1 | – | 8.6 | 6.3 | 5.6 | 2.3 | -2.2 | -6.1 | -6.2 | -6.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.9 | 3.1 | 1.6 | 2.7 | 2.3 | 2.8 | – | 4.2 | 1.9 | 1.4 | 1.4 | 1.1 | 0 | 0 | 0 |
Peroxisomes | 0.4 | 1.4 | 1.5 | 0.4 | -0.8 | -1.6 | – | -2.3 | -0.3 | 5.0 | 4.7 | 4.9 | -2.5 | 0 | -2.4 |
SpindlePole | 3.4 | 4.2 | 0.2 | 1.7 | 1.5 | -1.9 | – | -1.3 | -0.5 | -2.8 | -3.3 | -0.9 | -1.8 | -2.4 | -1.9 |
Vacuole | -1.4 | -1.9 | -0.5 | -1.5 | 0.7 | -1.0 | – | -2.6 | -0.3 | -2.3 | -0.5 | 0.2 | 0.7 | 0.7 | 1.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.672 | 6.2134 | 5.6583 | 4.7474 | 4.8962 | 5.3033 | 3.3342 | 4.7003 | 4.1153 | 4.1215 | 3.4879 | 4.1633 | 3.9676 | 5.3339 | 4.9706 | 3.7903 | 3.5796 | 4.4839 |
Actin | 0.0107 | 0.0064 | 0.0108 | 0.0035 | 0.1659 | 0.0143 | 0.0252 | 0.0061 | 0.0309 | 0.0023 | 0.0015 | 0.016 | 0.0266 | 0.006 | 0.0071 | 0.018 | 0.0305 | 0.0066 |
Bud | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.001 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0008 | 0.0005 | 0.0002 |
Bud Neck | 0.0074 | 0.0006 | 0.0011 | 0.0005 | 0.008 | 0.001 | 0.0009 | 0.0004 | 0.001 | 0.0003 | 0.0002 | 0.0008 | 0.0013 | 0.0006 | 0.0104 | 0.0235 | 0.001 | 0.0017 |
Bud Periphery | 0.0004 | 0.0002 | 0.0002 | 0.0004 | 0.0013 | 0.0006 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0015 | 0.0007 | 0.0004 |
Bud Site | 0.0082 | 0.0013 | 0.0006 | 0.0005 | 0.0137 | 0.0009 | 0.0079 | 0.0007 | 0.0018 | 0.0003 | 0.0001 | 0.0001 | 0.0018 | 0.0006 | 0.0006 | 0.0044 | 0.0015 | 0.0005 |
Cell Periphery | 0.0042 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0013 | 0.0003 | 0.0002 |
Cytoplasm | 0.0154 | 0.0204 | 0.0004 | 0.0004 | 0.0023 | 0.0018 | 0.0103 | 0.0057 | 0.0017 | 0.0004 | 0.0004 | 0.0002 | 0.0033 | 0.0007 | 0.0141 | 0.011 | 0.0089 | 0.0004 |
Cytoplasmic Foci | 0.0749 | 0.0661 | 0.0807 | 0.0522 | 0.0796 | 0.0366 | 0.0931 | 0.1157 | 0.0912 | 0.0991 | 0.0737 | 0.052 | 0.0878 | 0.1 | 0.0775 | 0.0931 | 0.0701 | 0.0401 |
Eisosomes | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0006 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0041 | 0.0008 | 0.0002 | 0.0007 | 0.0055 | 0.0011 | 0.0035 | 0.0012 | 0.0009 | 0.0002 | 0.0002 | 0.0003 | 0.0044 | 0.0005 | 0.0008 | 0.005 | 0.0065 | 0.0007 |
Endosome | 0.0867 | 0.1075 | 0.0954 | 0.1195 | 0.1131 | 0.0476 | 0.0892 | 0.1335 | 0.1282 | 0.1644 | 0.0893 | 0.0794 | 0.1592 | 0.1456 | 0.1442 | 0.206 | 0.1467 | 0.0807 |
Golgi | 0.0904 | 0.1943 | 0.1583 | 0.2287 | 0.1434 | 0.107 | 0.1742 | 0.2932 | 0.3096 | 0.2597 | 0.2912 | 0.2693 | 0.133 | 0.2221 | 0.2807 | 0.2563 | 0.2253 | 0.2004 |
Lipid Particles | 0.4899 | 0.4611 | 0.4457 | 0.405 | 0.349 | 0.6165 | 0.4148 | 0.3342 | 0.2391 | 0.3813 | 0.3601 | 0.4635 | 0.4188 | 0.437 | 0.337 | 0.1561 | 0.2658 | 0.4844 |
Mitochondria | 0.0358 | 0.0473 | 0.0346 | 0.0472 | 0.0179 | 0.0373 | 0.023 | 0.0127 | 0.046 | 0.0226 | 0.1192 | 0.0154 | 0.0729 | 0.031 | 0.0432 | 0.0732 | 0.0952 | 0.0569 |
None | 0.0098 | 0.0006 | 0.0002 | 0.0001 | 0.0014 | 0.0013 | 0.0036 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0001 | 0.0026 | 0.0001 | 0.0004 | 0.015 | 0.0056 | 0.0002 |
Nuclear Periphery | 0.0039 | 0.001 | 0.0002 | 0.0004 | 0.0141 | 0.0063 | 0.0078 | 0.0003 | 0.0019 | 0.0002 | 0.0002 | 0.0003 | 0.0065 | 0.0012 | 0.0005 | 0.0049 | 0.0176 | 0.002 |
Nucleolus | 0.0011 | 0.0004 | 0.0002 | 0.0003 | 0.0004 | 0.0019 | 0.0005 | 0.0001 | 0.0021 | 0.0002 | 0.0001 | 0.0001 | 0.0011 | 0.0006 | 0.0041 | 0.0239 | 0.0086 | 0.0107 |
Nucleus | 0.0033 | 0.0002 | 0.0001 | 0.0001 | 0.0011 | 0.0025 | 0.0027 | 0.0001 | 0.0019 | 0.0001 | 0 | 0 | 0.0057 | 0.0002 | 0.0007 | 0.0084 | 0.0266 | 0.0091 |
Peroxisomes | 0.1356 | 0.084 | 0.1666 | 0.1319 | 0.0725 | 0.0973 | 0.1341 | 0.0927 | 0.1298 | 0.0634 | 0.0602 | 0.0983 | 0.0515 | 0.0458 | 0.071 | 0.0678 | 0.0597 | 0.0923 |
Punctate Nuclear | 0.0129 | 0.0007 | 0.0007 | 0.0005 | 0.0042 | 0.0054 | 0.0028 | 0.0006 | 0.0064 | 0.0009 | 0.0003 | 0.0005 | 0.0029 | 0.001 | 0.002 | 0.0129 | 0.0165 | 0.0053 |
Vacuole | 0.0019 | 0.0037 | 0.0015 | 0.0036 | 0.0021 | 0.0154 | 0.002 | 0.0011 | 0.0028 | 0.0019 | 0.001 | 0.001 | 0.0104 | 0.0028 | 0.0023 | 0.0101 | 0.0062 | 0.0027 |
Vacuole Periphery | 0.0025 | 0.0028 | 0.0022 | 0.004 | 0.0023 | 0.0044 | 0.0032 | 0.0014 | 0.0026 | 0.0024 | 0.0018 | 0.0024 | 0.0087 | 0.0034 | 0.0029 | 0.0064 | 0.006 | 0.0046 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.8909 | 25.9937 | 29.5482 | 33.1688 | 34.0908 | 21.9508 | 30.2664 | 34.062 | 39.1556 | 37.1348 |
Translational Efficiency | 0.5023 | 0.4834 | 0.4444 | 0.4887 | 0.4012 | 0.6081 | 0.5278 | 0.4159 | 0.46 | 0.5063 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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