Standard name
Human Ortholog
Description Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats and FFAT motif; interacts with ER anchor Scs2p at the nucleus-vacuole junction; regulated by sterol binding; SWH1 has a paralog, OSH2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.11 0.06 0.07 0 0 0 0 – 0 0.06 0 0 0 0.11 0.13 0.12 0 0 0 0.06 0.1 0.1
Bud 0 0 0 0 0 0 0 0 0.05 – 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.11 0.09 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.1 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0.2 0 0.35 0.53 0.51 0.37 0.34 0.32 0.09 – 0.07 0.13 0.26 0.33 0.37 0.12 0.07 0.18 0.29 0.37 0.47 0.35 0.27 0.24
Golgi 0 0.05 0.16 0.06 0.08 0 0 0 0 – 0 0 0.07 0.15 0.18 0.62 0.69 0.59 0.35 0.38 0.2 0.27 0.32 0.38
Mitochondria 0.8 0.87 0.43 0.26 0.21 0.52 0.53 0.47 0.79 – 0.8 0.7 0.65 0.52 0.34 0.12 0.07 0.1 0.05 0.05 0.07 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0.07 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0.09 0 0.07 0.08 0 0.06 0.05 0.07 – 0.11 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.06 0 0.06 0.07 0.1 0.1 0.07 0.05 0 – 0 0.06 0.21 0.2 0.21 0 0 0 0.25 0.11 0.19 0.21 0.24 0.21
SpindlePole 0 0 0.1 0.22 0.24 0.11 0.15 0.14 0.05 – 0.07 0.09 0 0 0.08 0.05 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.06 0 0 0.05 0 0.08 0 – 0 0.06 0 0.05 0.07 0.09 0.09 0.13 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 11 3 26 12 18 4 9 14 5 – 10 17 10 6 5 14 12 14 7 2 3 15 44 50
Bud 3 2 3 1 3 5 12 13 13 – 12 24 10 9 3 0 3 0 0 0 1 0 3 1
Bud Neck 0 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 1 0 0 0 1 2 0 1 – 2 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 0 0 – 1 0 0 1 0 1 0 0 0 0 0 0 0 0
Cytoplasm 7 1 5 9 9 11 14 16 9 – 11 13 11 9 17 14 8 8 0 0 0 1 0 1
Endoplasmic Reticulum 5 13 15 6 4 9 8 6 5 – 8 9 9 6 4 6 4 4 0 0 3 2 6 7
Endosome 50 3 82 107 141 110 144 136 23 – 18 37 114 144 179 15 7 21 76 57 127 90 121 126
Golgi 5 6 38 12 21 3 4 13 3 – 1 11 31 66 89 80 65 70 90 58 56 71 144 195
Mitochondria 206 108 99 52 58 154 225 197 194 – 212 205 286 230 165 15 7 12 14 7 18 12 12 16
Nucleus 0 1 0 0 0 1 1 4 2 – 3 1 1 1 0 0 1 0 0 0 0 0 0 0
Nuclear Periphery 13 9 2 2 4 6 7 9 6 – 10 4 10 7 8 1 0 3 1 1 1 1 0 0
Nucleolus 30 11 4 15 21 12 27 23 17 – 30 14 16 16 15 5 3 3 0 0 0 0 1 0
Peroxisomes 16 3 15 15 27 30 31 21 8 – 6 17 94 89 102 1 0 1 64 17 52 56 106 107
SpindlePole 5 2 23 44 66 31 62 59 13 – 18 25 19 16 38 6 2 5 1 0 3 3 5 5
Vac/Vac Membrane 8 5 15 7 8 16 16 34 11 – 5 17 12 24 33 11 8 15 1 4 3 1 1 0
Unique Cell Count 256 124 232 202 274 294 423 423 246 265 293 440 442 485 129 94 119 263 153 274 262 451 521
Labelled Cell Count 359 168 328 282 381 393 562 545 310 347 396 623 624 658 169 120 156 263 153 274 262 451 521


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.4 8.4 8.2 7.7 7.9 6.6 6.7 7.0 6.0 – 5.5 6.3 9.0 9.2 10.3 15.4 16.1 15.0 8.4 8.2 8.8
Std Deviation (1e-4) 1.1 1.8 1.2 1.0 1.1 1.2 1.2 1.2 1.1 – 1.1 1.1 1.2 2.2 3.6 3.2 3.3 3.0 2.4 1.2 1.5
Intensity Change (Log2) – – – -0.1 -0.05 -0.32 -0.3 -0.24 -0.46 – -0.58 -0.39 0.13 0.16 0.32 0.91 0.97 0.87 0.03 0.0 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.9 -1.8 -4.8 -4.9 -4.0 -4.1 – -3.2 -2.2 -4.9 -5.7 -6.3 -0.1 0.4 0.2
Bud 0 0 0 1.3 1.4 2.4 – 2.1 3.6 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 1.4 0.8 1.1 0.8 1.1 1.0 – 1.3 1.4 0.3 0 1.0 3.5 0 0
Endoplasmic Reticulum -1.7 -3.0 -1.9 -3.0 -3.5 -2.4 – -1.8 -1.8 -2.9 -3.6 -4.4 -0.7 -0.8 -1.2
Endosome 3.7 3.6 0.5 -0.3 -0.8 -6.9 – -7.9 -6.2 -2.6 -0.7 0.4 -4.9 -5.1 -3.4
Golgi -3.4 -3.0 -6.5 -7.7 -6.1 -5.9 – -6.6 -4.9 -3.8 -0.5 0.6 8.9 9.3 8.2
Mitochondria -3.7 -5.2 2.2 2.6 1.0 8.1 – 8.6 6.3 5.6 2.3 -2.2 -6.1 -6.2 -6.2
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 2.1 0 0 0 0 0 0 0
Nucleolus 2.9 3.1 1.6 2.7 2.3 2.8 – 4.2 1.9 1.4 1.4 1.1 0 0 0
Peroxisomes 0.4 1.4 1.5 0.4 -0.8 -1.6 – -2.3 -0.3 5.0 4.7 4.9 -2.5 0 -2.4
SpindlePole 3.4 4.2 0.2 1.7 1.5 -1.9 – -1.3 -0.5 -2.8 -3.3 -0.9 -1.8 -2.4 -1.9
Vacuole -1.4 -1.9 -0.5 -1.5 0.7 -1.0 – -2.6 -0.3 -2.3 -0.5 0.2 0.7 0.7 1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.672 6.2134 5.6583 4.7474 4.8962 5.3033 3.3342 4.7003 4.1153 4.1215 3.4879 4.1633 3.9676 5.3339 4.9706 3.7903 3.5796 4.4839
Actin 0.0107 0.0064 0.0108 0.0035 0.1659 0.0143 0.0252 0.0061 0.0309 0.0023 0.0015 0.016 0.0266 0.006 0.0071 0.018 0.0305 0.0066
Bud 0.0002 0.0002 0.0001 0.0002 0.001 0.0003 0.0003 0.0001 0.0003 0.0001 0.0001 0.0001 0.0004 0.0001 0.0002 0.0008 0.0005 0.0002
Bud Neck 0.0074 0.0006 0.0011 0.0005 0.008 0.001 0.0009 0.0004 0.001 0.0003 0.0002 0.0008 0.0013 0.0006 0.0104 0.0235 0.001 0.0017
Bud Periphery 0.0004 0.0002 0.0002 0.0004 0.0013 0.0006 0.0004 0.0001 0.0004 0.0001 0.0001 0.0001 0.0006 0.0002 0.0002 0.0015 0.0007 0.0004
Bud Site 0.0082 0.0013 0.0006 0.0005 0.0137 0.0009 0.0079 0.0007 0.0018 0.0003 0.0001 0.0001 0.0018 0.0006 0.0006 0.0044 0.0015 0.0005
Cell Periphery 0.0042 0.0002 0.0001 0.0002 0.0004 0.0002 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001 0.0003 0.0002 0.0002 0.0013 0.0003 0.0002
Cytoplasm 0.0154 0.0204 0.0004 0.0004 0.0023 0.0018 0.0103 0.0057 0.0017 0.0004 0.0004 0.0002 0.0033 0.0007 0.0141 0.011 0.0089 0.0004
Cytoplasmic Foci 0.0749 0.0661 0.0807 0.0522 0.0796 0.0366 0.0931 0.1157 0.0912 0.0991 0.0737 0.052 0.0878 0.1 0.0775 0.0931 0.0701 0.0401
Eisosomes 0.0007 0.0001 0.0001 0.0001 0.0008 0.0001 0.0002 0 0.0002 0 0.0001 0.0001 0.0003 0.0001 0.0001 0.0006 0.0003 0.0001
Endoplasmic Reticulum 0.0041 0.0008 0.0002 0.0007 0.0055 0.0011 0.0035 0.0012 0.0009 0.0002 0.0002 0.0003 0.0044 0.0005 0.0008 0.005 0.0065 0.0007
Endosome 0.0867 0.1075 0.0954 0.1195 0.1131 0.0476 0.0892 0.1335 0.1282 0.1644 0.0893 0.0794 0.1592 0.1456 0.1442 0.206 0.1467 0.0807
Golgi 0.0904 0.1943 0.1583 0.2287 0.1434 0.107 0.1742 0.2932 0.3096 0.2597 0.2912 0.2693 0.133 0.2221 0.2807 0.2563 0.2253 0.2004
Lipid Particles 0.4899 0.4611 0.4457 0.405 0.349 0.6165 0.4148 0.3342 0.2391 0.3813 0.3601 0.4635 0.4188 0.437 0.337 0.1561 0.2658 0.4844
Mitochondria 0.0358 0.0473 0.0346 0.0472 0.0179 0.0373 0.023 0.0127 0.046 0.0226 0.1192 0.0154 0.0729 0.031 0.0432 0.0732 0.0952 0.0569
None 0.0098 0.0006 0.0002 0.0001 0.0014 0.0013 0.0036 0.0001 0.001 0.0001 0.0001 0.0001 0.0026 0.0001 0.0004 0.015 0.0056 0.0002
Nuclear Periphery 0.0039 0.001 0.0002 0.0004 0.0141 0.0063 0.0078 0.0003 0.0019 0.0002 0.0002 0.0003 0.0065 0.0012 0.0005 0.0049 0.0176 0.002
Nucleolus 0.0011 0.0004 0.0002 0.0003 0.0004 0.0019 0.0005 0.0001 0.0021 0.0002 0.0001 0.0001 0.0011 0.0006 0.0041 0.0239 0.0086 0.0107
Nucleus 0.0033 0.0002 0.0001 0.0001 0.0011 0.0025 0.0027 0.0001 0.0019 0.0001 0 0 0.0057 0.0002 0.0007 0.0084 0.0266 0.0091
Peroxisomes 0.1356 0.084 0.1666 0.1319 0.0725 0.0973 0.1341 0.0927 0.1298 0.0634 0.0602 0.0983 0.0515 0.0458 0.071 0.0678 0.0597 0.0923
Punctate Nuclear 0.0129 0.0007 0.0007 0.0005 0.0042 0.0054 0.0028 0.0006 0.0064 0.0009 0.0003 0.0005 0.0029 0.001 0.002 0.0129 0.0165 0.0053
Vacuole 0.0019 0.0037 0.0015 0.0036 0.0021 0.0154 0.002 0.0011 0.0028 0.0019 0.001 0.001 0.0104 0.0028 0.0023 0.0101 0.0062 0.0027
Vacuole Periphery 0.0025 0.0028 0.0022 0.004 0.0023 0.0044 0.0032 0.0014 0.0026 0.0024 0.0018 0.0024 0.0087 0.0034 0.0029 0.0064 0.006 0.0046

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.8909 25.9937 29.5482 33.1688 34.0908 21.9508 30.2664 34.062 39.1556 37.1348
Translational Efficiency 0.5023 0.4834 0.4444 0.4887 0.4012 0.6081 0.5278 0.4159 0.46 0.5063

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats and FFAT motif; interacts with ER anchor Scs2p at the nucleus-vacuole junction; regulated by sterol binding; SWH1 has a paralog, OSH2, that arose from the whole genome duplication
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Swh1

Swh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Swh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available