Standard name | |
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Human Ortholog | |
Description | Histone H2B; core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates reassembly after DNA replication, transcriptional activation, meiotic DSB formation and H3 methylation |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 3 | 0 | 11 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 80 | 18 | 14 | 42 | 43 | 69 | – | 222 | 177 | 127 | 372 | 106 | 40 | 31 | 21 | 55 | 19 | 26 | 0 | 0 | 0 | 0 | 0 | 4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 13 | 5 | 9 | 1 | 1 | 0 | 0 | 1 | 0 |
Endosome | 0 | 0 | 0 | 1 | 0 | 0 | – | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Mitochondria | 32 | 12 | 57 | 57 | 29 | 53 | – | 120 | 46 | 70 | 62 | 59 | 34 | 48 | 38 | 19 | 1 | 11 | 0 | 0 | 0 | 1 | 2 | 2 |
Nucleus | 264 | 118 | 120 | 249 | 291 | 311 | – | 465 | 286 | 221 | 96 | 248 | 213 | 250 | 232 | 105 | 37 | 44 | 242 | 119 | 84 | 81 | 177 | 199 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 5 | 1 | 2 | 8 | 13 |
Nucleolus | 33 | 25 | 58 | 69 | 44 | 95 | – | 151 | 77 | 91 | 76 | 87 | 54 | 70 | 65 | 22 | 11 | 15 | 12 | 7 | 28 | 19 | 31 | 75 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 2 | 0 | 2 | – | 7 | 4 | 1 | 5 | 4 | 0 | 4 | 1 | 2 | 2 | 0 | 1 | 1 | 1 | 1 | 3 | 11 |
Unique Cell Count | 294 | 132 | 152 | 290 | 330 | 388 | 639 | 424 | 315 | 485 | 328 | 244 | 306 | 294 | 145 | 56 | 72 | 284 | 143 | 134 | 114 | 242 | 336 | |
Labelled Cell Count | 411 | 174 | 249 | 420 | 408 | 530 | 968 | 594 | 510 | 624 | 507 | 344 | 403 | 362 | 216 | 75 | 105 | 284 | 143 | 134 | 114 | 242 | 336 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 185.7 | 162.4 | 113.2 | 139.4 | 156.9 | 141.8 | – | 163.5 | 164.4 | 150.8 | 268.4 | 148.6 | 157.1 | 150.1 | 136.6 | 191.0 | 161.8 | 169.1 | 160.3 | 161.4 | 142.5 |
Std Deviation (1e-4) | 50.3 | 60.5 | 46.0 | 59.0 | 55.2 | 56.1 | – | 63.0 | 70.4 | 63.3 | 150.9 | 63.0 | 53.9 | 56.6 | 52.4 | 71.8 | 86.4 | 74.7 | 51.5 | 58.7 | 57.1 |
Intensity Change (Log2) | – | – | – | 0.3 | 0.47 | 0.32 | – | 0.53 | 0.54 | 0.41 | 1.25 | 0.39 | 0.47 | 0.41 | 0.27 | 0.75 | 0.52 | 0.58 | 0.5 | 0.51 | 0.33 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.6 | 1.2 | 2.5 | – | 6.2 | 7.3 | 6.9 | 14.9 | 5.4 | 2.0 | 0.3 | -0.8 | 5.9 | 4.3 | 4.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.8 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -4.1 | -7.7 | -6.2 | – | -5.0 | -7.4 | -3.5 | -6.8 | -4.6 | -5.4 | -5.2 | -6.0 | -4.8 | -5.1 | -3.4 |
Nucleus | 1.9 | 2.7 | 0.3 | – | -1.6 | -2.7 | -2.0 | -13.4 | -0.8 | 2.2 | 0.7 | 0 | -1.3 | -1.9 | -2.8 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -3.2 | -6.2 | -3.2 | – | -3.7 | -5.0 | -2.0 | -5.9 | -2.6 | -3.4 | -3.4 | -3.6 | -4.5 | -2.5 | -2.6 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0.0011 | 0 | 0.0001 | 0 | – | – | – | – | – | – |
Bud | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | – | – | – | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | – | – | – | – | – | – |
Bud Periphery | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0007 | 0 | 0.0001 | 0 | – | – | – | – | – | – |
Bud Site | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0026 | 0 | 0.0001 | 0 | – | – | – | – | – | – |
Cell Periphery | 0.0002 | 0.0001 | 0 | 0 | 0.0006 | 0.0002 | 0.0001 | 0 | 0.0004 | 0 | 0.001 | 0.0001 | – | – | – | – | – | – |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0.002 | 0 | 0 | 0.0003 | 0 | 0 | 0 | – | – | – | – | – | – |
Cytoplasmic Foci | 0 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0 | – | – | – | – | – | – |
Eisosomes | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0.0013 | 0 | 0 | 0.0003 | 0 | 0 | 0 | – | – | – | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0.003 | 0 | 0 | 0.0003 | 0 | 0 | 0 | – | – | – | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | – | – | – | – | – | – |
Lipid Particles | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0005 | 0 | – | – | – | – | – | – |
Mitochondria | 0.0076 | 0.0021 | 0.0022 | 0.0016 | 0.1029 | 0.0017 | 0.0027 | 0.0005 | 0.0026 | 0.0015 | 0.097 | 0.0007 | – | – | – | – | – | – |
None | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | – | – | – | – | – | – |
Nuclear Periphery | 0.0002 | 0 | 0 | 0 | 0 | 0.0038 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0 | – | – | – | – | – | – |
Nucleolus | 0.3666 | 0.1398 | 0.1478 | 0.0883 | 0.6012 | 0.2985 | 0.2164 | 0.098 | 0.1116 | 0.1442 | 0.6002 | 0.2648 | – | – | – | – | – | – |
Nucleus | 0.6239 | 0.8571 | 0.8497 | 0.9099 | 0.292 | 0.6868 | 0.78 | 0.9014 | 0.8776 | 0.8541 | 0.2965 | 0.7341 | – | – | – | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0 | – | – | – | – | – | – |
Punctate Nuclear | 0.0002 | 0.0005 | 0 | 0 | 0 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | – | – | – | – | – | – |
Vacuole | 0.0002 | 0.0001 | 0 | 0 | 0.0005 | 0.0006 | 0.0001 | 0 | 0.0007 | 0 | 0.0008 | 0.0001 | – | – | – | – | – | – |
Vacuole Periphery | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0016 | 0.0005 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0027 | 0.0001 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 785.7577 | 682.4926 | 149.7137 | 118.657 | 847.168 | 462.2822 | 472.8474 | 266.0209 | 190.7672 | 675.3592 |
Translational Efficiency | 2.4524 | 2.4373 | 1.8416 | 2.5569 | 2.3445 | 3.675 | 2.4106 | 2.8764 | 2.5807 | 2.8194 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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