Standard name | |
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Human Ortholog | |
Description | Mitogen-activated serine/threonine protein kinase involved in mating; phosphoactivated by Ste7p; substrates include Ste12p, Far1p, Bni1p, Sst2p; inhibits invasive growth during mating by phosphorylating Tec1p, promoting its; inhibits recruitment of Ste5p, Cdc42p-mediated asymmetry and mating morphogenesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 3 | 6 | 4 | 4 | 11 | 3 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 10 | 19 | 9 | 7 | 15 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 3 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 26 | 47 | 36 | 38 | 65 | 75 | 72 | 87 | 102 | 88 | 40 | 54 | 40 | 69 | 67 | 92 | 72 | 98 | 8 | 18 | 50 | 23 | 30 | 19 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 11 | 5 | 8 | 1 | 0 | 3 | 1 | 3 | 3 |
Endosome | 0 | 1 | 2 | 0 | 13 | 21 | 6 | 16 | 4 | 15 | 0 | 0 | 1 | 2 | 1 | 10 | 8 | 2 | 1 | 3 | 7 | 1 | 3 | 5 |
Golgi | 0 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 1 | 5 | 2 | 4 | 0 | 0 | 1 | 0 | 3 | 9 |
Mitochondria | 4 | 1 | 1 | 1 | 1 | 20 | 73 | 27 | 85 | 64 | 79 | 49 | 1 | 0 | 0 | 3 | 4 | 2 | 1 | 0 | 7 | 1 | 0 | 0 |
Nucleus | 23 | 50 | 59 | 84 | 59 | 81 | 69 | 91 | 97 | 106 | 56 | 67 | 16 | 31 | 26 | 34 | 23 | 18 | 93 | 179 | 114 | 45 | 78 | 66 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 |
Nucleolus | 0 | 1 | 0 | 1 | 1 | 0 | 5 | 1 | 2 | 4 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 2 | 2 | 2 | 3 | 2 | 2 | 3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 3 | 4 | 2 | 2 | 5 |
Vac/Vac Membrane | 76 | 165 | 121 | 64 | 152 | 138 | 84 | 103 | 88 | 83 | 35 | 53 | 55 | 86 | 71 | 209 | 173 | 136 | 15 | 32 | 34 | 19 | 44 | 55 |
Unique Cell Count | 113 | 220 | 201 | 168 | 261 | 285 | 269 | 299 | 329 | 330 | 180 | 198 | 101 | 156 | 143 | 297 | 244 | 234 | 143 | 264 | 245 | 110 | 189 | 189 |
Labelled Cell Count | 129 | 269 | 219 | 189 | 294 | 340 | 319 | 329 | 386 | 375 | 214 | 229 | 118 | 191 | 166 | 370 | 292 | 272 | 143 | 264 | 245 | 110 | 189 | 189 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.1 | 16.6 | 10.3 | 10.5 | 9.4 | 7.7 | 6.5 | 7.4 | 6.1 | 6.5 | 5.6 | 6.1 | 13.6 | 14.0 | 13.0 | 11.6 | 11.5 | 10.8 | 22.5 | 30.4 | 40.0 |
Std Deviation (1e-4) | 3.0 | 3.8 | 2.0 | 1.9 | 1.6 | 1.9 | 1.5 | 1.6 | 1.4 | 1.5 | 1.3 | 1.2 | 3.2 | 3.0 | 2.6 | 2.1 | 2.2 | 2.0 | 8.1 | 10.8 | 13.9 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.13 | -0.41 | -0.66 | -0.47 | -0.75 | -0.67 | -0.88 | -0.76 | 0.41 | 0.44 | 0.34 | 0.18 | 0.16 | 0.08 | 1.13 | 1.56 | 1.96 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.1 | 1.8 | 2.2 | 2.3 | 2.8 | 3.3 | 2.3 | 1.1 | 2.2 | 4.1 | 5.4 | 5.8 | 3.3 | 2.8 | 5.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2.4 | 3.3 | 0 | 2.6 | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.5 | 7.8 | 4.1 | 7.7 | 6.4 | 10.4 | 7.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 4.1 | -1.6 | -0.2 | -0.9 | 0.3 | 0 | 0.7 | 0.4 | 1.0 | -2.6 | -2.0 | -2.4 | -5.0 | -5.4 | -5.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -4.2 | -0.4 | -2.6 | -6.3 | -5.7 | -7.6 | -8.1 | -8.1 | -6.7 | -1.0 | -1.0 | -1.9 | 2.4 | 2.4 | -0.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.7127 | 10.1855 | 8.24 | 8.5165 | 7.4308 | 8.5179 | 7.4669 | 9.154 | 8.1443 | 7.7605 | 6.7706 | 7.6637 | 5.3523 | 7.1821 | 6.934 | 4.9172 | 4.0845 | 6.6238 |
Actin | 0.0095 | 0.0011 | 0.0022 | 0.0028 | 0.0044 | 0.0016 | 0.0025 | 0.0001 | 0.0003 | 0 | 0.0042 | 0 | 0.0686 | 0.0019 | 0.034 | 0.0018 | 0.0172 | 0.0005 |
Bud | 0.0011 | 0.0001 | 0.0012 | 0.0003 | 0.0009 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0009 | 0 | 0.0027 | 0.0004 | 0.0003 | 0.003 | 0.0009 | 0.0002 |
Bud Neck | 0.0047 | 0.0003 | 0.0005 | 0.0015 | 0.0186 | 0.0029 | 0.0011 | 0.0003 | 0.0003 | 0.0002 | 0.0017 | 0.0016 | 0.0027 | 0.0003 | 0.0004 | 0.0072 | 0.0011 | 0.0017 |
Bud Periphery | 0.0023 | 0 | 0.0005 | 0.0004 | 0.0031 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0 | 0.0005 | 0 | 0.0027 | 0.0004 | 0.0002 | 0.0041 | 0.0014 | 0.0001 |
Bud Site | 0.0022 | 0.0014 | 0.001 | 0.0006 | 0.0016 | 0.0003 | 0.0007 | 0.0003 | 0.0003 | 0 | 0.0011 | 0 | 0.0228 | 0.0008 | 0.0006 | 0.0009 | 0.0026 | 0.0002 |
Cell Periphery | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0 |
Cytoplasm | 0.041 | 0.0263 | 0.0297 | 0.0058 | 0.0355 | 0.0226 | 0.0261 | 0.0284 | 0.0106 | 0.0032 | 0.0119 | 0.0095 | 0.0405 | 0.035 | 0.0387 | 0.0423 | 0.0124 | 0.0359 |
Cytoplasmic Foci | 0.0178 | 0.0033 | 0.0076 | 0.0042 | 0.0291 | 0.0075 | 0.0053 | 0.0002 | 0.0009 | 0 | 0.0007 | 0.0001 | 0.0528 | 0.0036 | 0.0092 | 0.004 | 0.0242 | 0.0213 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0127 | 0.0013 | 0.0019 | 0.0003 | 0.0095 | 0.0018 | 0.0015 | 0.0005 | 0.0005 | 0 | 0.0024 | 0.0002 | 0.0096 | 0.0019 | 0.0034 | 0.0049 | 0.0037 | 0.0009 |
Endosome | 0.031 | 0.0042 | 0.0108 | 0.0167 | 0.0332 | 0.0092 | 0.004 | 0.0002 | 0.0022 | 0 | 0.0157 | 0.0003 | 0.0489 | 0.0086 | 0.0201 | 0.0813 | 0.0842 | 0.0019 |
Golgi | 0.0122 | 0.0005 | 0.0051 | 0.0168 | 0.0029 | 0.0065 | 0.0007 | 0 | 0.0007 | 0 | 0.0017 | 0 | 0.0149 | 0.0075 | 0.0156 | 0.0102 | 0.0362 | 0.0006 |
Lipid Particles | 0.0077 | 0.0016 | 0.0024 | 0.0014 | 0.0096 | 0.0053 | 0.0017 | 0 | 0.0004 | 0 | 0.0019 | 0 | 0.0303 | 0.0005 | 0.0023 | 0.002 | 0.0356 | 0.0026 |
Mitochondria | 0.0165 | 0.0003 | 0.0073 | 0.0157 | 0.0028 | 0.0027 | 0.0042 | 0.0001 | 0.0024 | 0 | 0.0014 | 0.0001 | 0.0184 | 0.0029 | 0.0391 | 0.0129 | 0.1599 | 0.0007 |
None | 0.0111 | 0.0027 | 0.0121 | 0.0001 | 0.0039 | 0.0004 | 0.0007 | 0.0001 | 0.0005 | 0 | 0.0011 | 0.0001 | 0.0401 | 0.033 | 0.0003 | 0.001 | 0.0007 | 0.0023 |
Nuclear Periphery | 0.0404 | 0.0145 | 0.0099 | 0.0041 | 0.0191 | 0.0081 | 0.0108 | 0.0077 | 0.0046 | 0.0021 | 0.0319 | 0.0065 | 0.0339 | 0.0131 | 0.0086 | 0.0107 | 0.0119 | 0.0118 |
Nucleolus | 0.0028 | 0.0015 | 0.0017 | 0.0009 | 0.0037 | 0.0023 | 0.002 | 0.0011 | 0.0012 | 0.0006 | 0.002 | 0.0016 | 0.0081 | 0.0011 | 0.0005 | 0.0014 | 0.0012 | 0.0016 |
Nucleus | 0.7524 | 0.9343 | 0.897 | 0.9215 | 0.7943 | 0.9172 | 0.9253 | 0.9506 | 0.9639 | 0.993 | 0.9128 | 0.972 | 0.5595 | 0.8844 | 0.8224 | 0.7923 | 0.586 | 0.9102 |
Peroxisomes | 0.0072 | 0.0006 | 0.0017 | 0.0008 | 0.0039 | 0.0043 | 0.0067 | 0 | 0.0011 | 0 | 0.0003 | 0 | 0.0129 | 0.0003 | 0.002 | 0.0005 | 0.0063 | 0.0029 |
Punctate Nuclear | 0.0161 | 0.0045 | 0.0048 | 0.0032 | 0.0159 | 0.0027 | 0.0029 | 0.0091 | 0.0088 | 0.0008 | 0.0053 | 0.0075 | 0.008 | 0.0022 | 0.0011 | 0.001 | 0.0084 | 0.0034 |
Vacuole | 0.0086 | 0.0013 | 0.002 | 0.0023 | 0.0063 | 0.0035 | 0.0018 | 0.0009 | 0.0009 | 0 | 0.001 | 0.0004 | 0.0144 | 0.0015 | 0.0005 | 0.0106 | 0.0037 | 0.0009 |
Vacuole Periphery | 0.0024 | 0.0002 | 0.0006 | 0.0005 | 0.0008 | 0.0007 | 0.0009 | 0.0001 | 0.0002 | 0 | 0.0012 | 0.0001 | 0.0069 | 0.0005 | 0.0004 | 0.0078 | 0.002 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.5848 | 8.3591 | 14.4733 | 14.0482 | 21.5535 | 8.3073 | 14.7908 | 15.2119 | 11.9107 | 16.4056 |
Translational Efficiency | 0.943 | 1.4279 | 0.8366 | 0.8677 | 0.9195 | 1.3604 | 0.6301 | 0.762 | 0.9311 | 1.0398 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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