Standard name | |
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Human Ortholog | |
Description | Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 4 | 4 | 0 | 0 | 0 |
Bud | 1 | 0 | 0 | 0 | 1 | 1 | 9 | 3 | 4 | 8 | 2 | 6 | 0 | 1 | 2 | 0 | 0 | 0 | 2 | 5 | 5 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 2 | 0 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 3 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 276 | 312 | 188 | 116 | 138 | 173 | 184 | 144 | 110 | 114 | 72 | 70 | 110 | 167 | 220 | 406 | 203 | 174 | 244 | 255 | 120 | 67 | 85 | 79 |
Endoplasmic Reticulum | 0 | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 43 | 24 | 15 | 1 | 0 | 5 | 2 | 0 | 0 |
Endosome | 0 | 0 | 8 | 1 | 4 | 3 | 3 | 5 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 16 | 3 | 2 | 3 | 3 | 1 | 0 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 2 | 1 | 3 | 2 | 1 | 2 | 0 | 0 | 1 |
Mitochondria | 116 | 21 | 17 | 47 | 29 | 180 | 222 | 210 | 206 | 281 | 189 | 214 | 1 | 2 | 7 | 23 | 7 | 6 | 18 | 21 | 46 | 5 | 4 | 2 |
Nucleus | 3 | 7 | 2 | 4 | 4 | 30 | 43 | 21 | 32 | 59 | 37 | 39 | 10 | 13 | 10 | 27 | 25 | 19 | 19 | 48 | 13 | 3 | 3 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 1 | 0 | 2 | 3 | 1 | 0 | 8 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 8 | 0 | 1 | 5 | 2 | 0 | 0 | 0 | 2 | 2 | 0 | 8 | 13 | 3 | 2 | 2 | 1 |
Vac/Vac Membrane | 41 | 99 | 56 | 17 | 28 | 43 | 67 | 29 | 23 | 31 | 28 | 39 | 30 | 30 | 53 | 169 | 120 | 72 | 77 | 90 | 49 | 11 | 7 | 17 |
Unique Cell Count | 362 | 396 | 244 | 158 | 179 | 354 | 424 | 339 | 293 | 384 | 258 | 275 | 141 | 202 | 271 | 554 | 314 | 248 | 402 | 466 | 269 | 94 | 114 | 114 |
Labelled Cell Count | 437 | 440 | 275 | 191 | 207 | 434 | 540 | 423 | 379 | 505 | 342 | 372 | 156 | 215 | 294 | 689 | 387 | 294 | 402 | 466 | 269 | 94 | 114 | 114 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.3 | 4.2 | 4.2 | 3.6 | 4.2 | 3.4 | 3.3 | 3.3 | 3.2 | 2.9 | 3.1 | 2.9 | 4.6 | 4.5 | 4.4 | 5.5 | 6.1 | 5.6 | 4.3 | 4.3 | 5.6 |
Std Deviation (1e-4) | 0.7 | 0.8 | 1.2 | 1.3 | 1.9 | 1.3 | 1.7 | 1.1 | 1.3 | 0.8 | 1.0 | 1.1 | 1.3 | 1.6 | 1.4 | 1.3 | 1.9 | 1.4 | 1.5 | 1.7 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.23 | -0.02 | -0.33 | -0.36 | -0.35 | -0.41 | -0.57 | -0.47 | -0.54 | 0.13 | 0.07 | 0.06 | 0.36 | 0.53 | 0.4 | 0.01 | 0.01 | 0.41 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 2.2 | 3.8 | 1.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.287 | -0.2139 | -0.0566 | -0.2207 | 0.1189 | 0.0649 | 2.4349 | 2.8634 | 2.7501 | 2.3957 | 2.8884 | 2.9922 | 3.3628 | 3.3052 | 3.1097 | 3.2251 | 4.6332 | 3.2762 |
Actin | 0.0534 | 0.0001 | 0.0338 | 0.0008 | 0.0038 | 0.0004 | 0.0026 | 0.0003 | 0.0036 | 0.0366 | 0.0049 | 0.0012 | 0.0236 | 0.0002 | 0.001 | 0.0001 | 0.0003 | 0.0055 |
Bud | 0.0003 | 0.0004 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0005 | 0.0006 | 0.0009 | 0.0002 | 0.0007 | 0.0006 | 0.0013 | 0.0011 | 0.003 | 0 | 0.0001 | 0.0001 |
Bud Neck | 0.0028 | 0.0005 | 0.001 | 0.0009 | 0.0151 | 0.0024 | 0.0016 | 0.0006 | 0.0011 | 0.0006 | 0.0044 | 0.0049 | 0.0123 | 0.0002 | 0.0004 | 0.0005 | 0.0021 | 0.0098 |
Bud Periphery | 0.0008 | 0.0017 | 0.0003 | 0 | 0.0012 | 0.0001 | 0.0011 | 0.0006 | 0.0017 | 0.0004 | 0.0024 | 0.0021 | 0.0017 | 0.0004 | 0.0011 | 0 | 0.0001 | 0.0001 |
Bud Site | 0.0024 | 0.0017 | 0.0037 | 0.0002 | 0.0277 | 0.0003 | 0.0023 | 0.0106 | 0.002 | 0.0006 | 0.0153 | 0.0003 | 0.0038 | 0.0007 | 0.0118 | 0.0001 | 0.0002 | 0.0001 |
Cell Periphery | 0.0008 | 0.0008 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0011 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.1151 | 0.115 | 0.118 | 0.248 | 0.034 | 0.1429 | 0.1276 | 0.1683 | 0.1091 | 0.1107 | 0.1812 | 0.1477 | 0.1142 | 0.2008 | 0.1273 | 0.1604 | 0.1411 | 0.2143 |
Cytoplasmic Foci | 0.0174 | 0.0067 | 0.011 | 0.0048 | 0.0054 | 0.0164 | 0.0142 | 0.0053 | 0.0046 | 0.0027 | 0.0104 | 0.007 | 0.0094 | 0.0045 | 0.0108 | 0.0044 | 0.0044 | 0.0113 |
Eisosomes | 0.0014 | 0.0002 | 0.0003 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0038 | 0.0028 | 0.0072 | 0.0134 | 0.0009 | 0.0041 | 0.0048 | 0.0022 | 0.0023 | 0.0019 | 0.0028 | 0.0014 | 0.0062 | 0.0034 | 0.0034 | 0.0018 | 0.0026 | 0.0029 |
Endosome | 0.0107 | 0.0016 | 0.0103 | 0.0047 | 0.002 | 0.0034 | 0.0125 | 0.0029 | 0.005 | 0.0025 | 0.0096 | 0.0022 | 0.0124 | 0.0029 | 0.0024 | 0.0026 | 0.002 | 0.0042 |
Golgi | 0.0071 | 0.0001 | 0.0031 | 0.0002 | 0.0013 | 0.0001 | 0.0014 | 0.0002 | 0.0008 | 0.0012 | 0.0018 | 0.0002 | 0.0018 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0004 |
Lipid Particles | 0.0121 | 0.0004 | 0.0054 | 0.0006 | 0.0016 | 0.0008 | 0.0039 | 0.0004 | 0.0014 | 0.001 | 0.0046 | 0.0005 | 0.0032 | 0.0004 | 0.001 | 0.0002 | 0.0006 | 0.0006 |
Mitochondria | 0.003 | 0.0004 | 0.016 | 0.0011 | 0.0043 | 0.0008 | 0.0026 | 0.001 | 0.0064 | 0.0012 | 0.0022 | 0.0008 | 0.0025 | 0.0004 | 0.0013 | 0.0006 | 0.0004 | 0.0006 |
None | 0.5653 | 0.6267 | 0.5249 | 0.4685 | 0.857 | 0.5073 | 0.5584 | 0.5511 | 0.552 | 0.5364 | 0.4076 | 0.4841 | 0.5958 | 0.6979 | 0.6458 | 0.6921 | 0.7235 | 0.4436 |
Nuclear Periphery | 0.0098 | 0.0028 | 0.0219 | 0.0131 | 0.0023 | 0.0076 | 0.0116 | 0.0073 | 0.0139 | 0.0107 | 0.0124 | 0.0068 | 0.0221 | 0.0059 | 0.0092 | 0.0054 | 0.0088 | 0.0087 |
Nucleolus | 0.0043 | 0.007 | 0.0033 | 0.006 | 0.0045 | 0.0049 | 0.0036 | 0.0037 | 0.0053 | 0.0023 | 0.01 | 0.0052 | 0.0101 | 0.0017 | 0.002 | 0.0035 | 0.0018 | 0.0026 |
Nucleus | 0.1175 | 0.2031 | 0.193 | 0.2152 | 0.0093 | 0.2851 | 0.2143 | 0.2069 | 0.2441 | 0.261 | 0.2355 | 0.2689 | 0.1264 | 0.0597 | 0.1586 | 0.0978 | 0.0878 | 0.2293 |
Peroxisomes | 0.0324 | 0.0002 | 0.0303 | 0.0002 | 0.0046 | 0.0007 | 0.0081 | 0.002 | 0.0014 | 0.001 | 0.0214 | 0.0009 | 0.0015 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0.0002 |
Punctate Nuclear | 0.0103 | 0.0208 | 0.0118 | 0.0197 | 0.0217 | 0.0193 | 0.024 | 0.0329 | 0.0409 | 0.0279 | 0.0534 | 0.0639 | 0.0438 | 0.0159 | 0.018 | 0.0291 | 0.0228 | 0.0642 |
Vacuole | 0.0281 | 0.0067 | 0.0035 | 0.0018 | 0.0012 | 0.0028 | 0.0036 | 0.0026 | 0.0021 | 0.0007 | 0.0175 | 0.0011 | 0.0053 | 0.0035 | 0.0011 | 0.0009 | 0.001 | 0.0012 |
Vacuole Periphery | 0.0014 | 0.0004 | 0.0008 | 0.0004 | 0.0002 | 0.0003 | 0.0009 | 0.0003 | 0.0007 | 0.0003 | 0.0006 | 0.0002 | 0.0018 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 4.9417 | 5.0486 | 2.3414 | 5.8033 | 5.0341 | 2.8222 | 4.3071 | 3.6606 | 5.2829 | 6.3054 |
Translational Efficiency | 0.7118 | 0.5899 | 1.2074 | 0.6926 | 0.7062 | 1.6739 | 0.6828 | 1.1843 | 0.6121 | 0.5594 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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