Standard name
Human Ortholog
Description Protein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.11 0.06 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.19 0.31 0.25 0.26 0.46 0.2 0.14 0.13 0.13 0.13 0.11 0.11 0.19 0.23 0.3 0.35 0.33 0.3 0.13 0.16 0.11 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0.05 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.06 0.17 0.15 0.22 0.24 0.21 0.26 0.2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.68 0.58 0.53 0.54 0.27 0.46 0.53 0.44 0.45 0.43 0.45 0.43 0.73 0.56 0.47 0.43 0.44 0.5 0.64 0.55 0.4 0.56 0.39 0.36
Nuclear Periphery 0 0 0 0 0 0 0.05 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.09 0 0.14 0.19 0.18 0.25 0.25 0.3 0.36 0.32 0.33 0.35 0.06 0.09 0.12 0.07 0.08 0.09 0.07 0.06 0.27 0.19 0.28 0.22
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0
Vac/Vac Membrane 0 0.07 0 0.06 0.07 0.05 0 0.05 0 0.05 0 0.06 0 0.09 0.09 0.13 0.14 0.1 0 0 0 0.09 0.17 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0
Bud 0 3 1 1 1 8 11 15 8 9 7 2 0 0 0 0 0 1 6 39 11 9 3 5
Bud Neck 0 0 0 0 1 2 4 6 4 7 0 4 0 0 0 0 0 0 0 4 1 0 1 4
Bud Site 0 0 0 0 0 6 5 6 7 12 11 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 6 3 1 3 1 4 1 3 1 0 1 7 6 3 0 0 0 0 0 0
Cytoplasm 21 101 47 55 130 77 56 64 52 55 44 40 30 93 98 73 64 60 15 56 23 12 0 1
Endoplasmic Reticulum 0 0 3 0 0 1 1 2 0 4 4 2 0 0 0 1 4 3 0 1 2 3 0 0
Endosome 4 9 9 7 14 8 8 7 0 8 2 3 3 22 18 5 5 4 1 4 3 8 23 38
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 3 1 3 1 0 0 0 1 8
Mitochondria 2 3 4 4 17 66 60 109 95 92 108 73 0 2 0 4 3 5 0 3 1 5 11 22
Nucleus 74 192 101 114 75 178 214 222 174 183 187 159 117 225 152 90 86 100 74 195 82 188 175 190
Nuclear Periphery 0 1 4 7 2 16 19 14 12 24 10 7 1 8 8 3 1 4 1 0 3 3 14 15
Nucleolus 10 12 27 39 51 96 101 153 139 139 139 128 10 36 38 15 16 17 8 21 55 64 128 118
Peroxisomes 0 0 0 1 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 1 1 1 0 3 1 2 1 3 0 3 0 0 0 1 2 13 12 8 10 18
Vac/Vac Membrane 4 22 8 13 19 18 15 25 12 20 9 21 7 36 28 27 28 19 1 11 3 28 74 89
Unique Cell Count 109 330 189 210 282 389 403 505 391 429 415 366 161 405 322 209 194 200 115 356 204 338 450 529
Labelled Cell Count 115 343 204 242 318 481 496 629 505 560 523 446 169 426 343 228 215 220 115 356 204 338 450 529


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.1 9.2 8.9 8.9 8.5 7.3 7.9 7.7 7.4 7.6 7.5 7.6 9.9 10.1 10.5 13.0 13.0 12.9 9.1 9.8 10.0
Std Deviation (1e-4) 2.3 1.8 1.9 2.5 2.4 1.9 2.3 2.0 2.0 2.2 1.9 2.1 2.0 2.3 2.5 3.3 3.1 3.3 1.7 1.6 1.8
Intensity Change (Log2) – – – 0.0 -0.05 -0.28 -0.17 -0.2 -0.26 -0.22 -0.24 -0.21 0.17 0.19 0.24 0.56 0.55 0.55 0.04 0.14 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 4.7 -1.4 -3.3 -3.9 -3.5 -3.7 -4.5 -4.3 -1.4 -0.5 1.3 2.2 1.8 1.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -0.7 0.1 -1.8 -1.9 0 0 -2.0 0 0 -1.5 0.3 0.4 -1.3 -1.1 -1.5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.0 5.1 4.7 6.2 6.7 6.1 7.0 5.8 0 0 0 0 0 0
Nucleus 0.2 -5.9 -1.7 -0.1 -2.2 -2.0 -2.5 -1.9 -2.2 3.7 0.5 -1.4 -2.1 -1.8 -0.7
Nuclear Periphery 0.7 0 1.2 1.5 0 0.7 1.9 0 0 0 0 0 0 0 0
Nucleolus 1.2 1.1 2.9 3.0 4.3 5.3 4.7 4.9 5.1 -2.4 -2.0 -0.8 -2.3 -1.9 -1.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.9 1.1 0.2 -0.3 0.4 -0.7 0.2 -1.4 0.8 0.1 2.0 1.9 3.1 3.4 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4955 3.1073 3.2297 2.8103 2.1096 3.473 5.1991 5.4903 5.204 4.0687 3.75 4.9087 3.0169 4.6416 3.1649 2.9764 3.09 3.915
Actin 0.0243 0.0022 0.0025 0.0029 0.0023 0.0005 0.0164 0.0036 0.01 0.0193 0.047 0.0021 0.0012 0.0008 0.0479 0.0001 0.0006 0.0123
Bud 0.0001 0.0192 0.0362 0.067 0.0375 0.0023 0.0015 0.0215 0.0455 0.0774 0.0576 0.0083 0.0001 0.0035 0.0228 0.0003 0.01 0.0011
Bud Neck 0.0003 0.0019 0.0041 0.0192 0.0046 0.0067 0.0025 0.0027 0.0058 0.0051 0.0034 0.0081 0.0003 0.0008 0.002 0.0002 0.0021 0.002
Bud Periphery 0.0002 0.0091 0.0251 0.0549 0.0522 0.0027 0.0021 0.0127 0.0384 0.0553 0.0703 0.0096 0.0001 0.0016 0.0146 0.0001 0.0089 0.0005
Bud Site 0.0003 0.0339 0.0254 0.0315 0.0059 0.0024 0.0062 0.0563 0.0363 0.0207 0.0095 0.0013 0.0007 0.0037 0.0118 0.0002 0.0021 0.0004
Cell Periphery 0 0.0008 0.0013 0.0033 0.0028 0.0004 0.0005 0.0014 0.0018 0.0013 0.0021 0.0004 0 0.0001 0.0005 0 0.0009 0.0001
Cytoplasm 0.0276 0.2454 0.4469 0.4951 0.7209 0.127 0.0144 0.1572 0.2807 0.5977 0.5157 0.0821 0.0064 0.2326 0.5326 0.9597 0.8993 0.1264
Cytoplasmic Foci 0.0035 0.0082 0.0201 0.0097 0.0206 0.0049 0.0134 0.012 0.0222 0.0226 0.0421 0.0036 0.0518 0.001 0.0284 0.0114 0.0057 0.0082
Eisosomes 0.0002 0 0 0 0 0 0.0001 0.0001 0.0001 0.0001 0.0004 0 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.002 0.0078 0.0064 0.0065 0.0036 0.0016 0.0068 0.0035 0.0086 0.0058 0.0072 0.002 0.0002 0.0011 0.0119 0.0012 0.0135 0.0007
Endosome 0.0028 0.0032 0.0278 0.0241 0.1043 0.0073 0.0125 0.0126 0.0299 0.0327 0.0486 0.0085 0.0033 0.0004 0.0364 0.0179 0.0078 0.0064
Golgi 0.0013 0.0006 0.001 0.0007 0.0037 0.0002 0.0057 0.0013 0.0029 0.0144 0.0245 0.0012 0.0032 0.0001 0.0025 0.0006 0.0003 0.0031
Lipid Particles 0.0036 0.0018 0.0025 0.0002 0.0002 0.0003 0.0151 0.0009 0.013 0.0156 0.062 0.001 0.009 0.0001 0.0105 0 0.0002 0.0052
Mitochondria 0.0008 0.001 0.0017 0.0018 0.0007 0.0004 0.0071 0.0096 0.0094 0.0076 0.0067 0.0009 0.0013 0.001 0.0043 0.0001 0.0003 0.0008
None 0.0071 0.0103 0.0087 0.0358 0.003 0.0005 0.0033 0.0129 0.0067 0.0087 0.014 0.001 0.0006 0.0009 0.091 0.0024 0.004 0.001
Nuclear Periphery 0.0094 0.0183 0.0148 0.0099 0.0019 0.0046 0.0125 0.0169 0.0351 0.0084 0.0123 0.0133 0.0022 0.0165 0.035 0.0004 0.0082 0.0062
Nucleolus 0.0194 0.0391 0.0392 0.0076 0.0014 0.0822 0.0762 0.081 0.0717 0.0034 0.0036 0.1249 0.0192 0.017 0.0017 0 0.0004 0.0415
Nucleus 0.8879 0.5694 0.2772 0.1768 0.0121 0.7444 0.7748 0.5419 0.3025 0.0576 0.031 0.7091 0.8646 0.6267 0.1045 0.0007 0.0105 0.7577
Peroxisomes 0.002 0.0011 0.0005 0.0003 0.0002 0.0001 0.0105 0.0029 0.0012 0.0098 0.0144 0.0004 0.0231 0.0002 0.0048 0 0.0001 0.0065
Punctate Nuclear 0.0062 0.0222 0.0308 0.0031 0.0004 0.0072 0.0084 0.0321 0.0525 0.0059 0.0066 0.018 0.0119 0.091 0.0042 0.0001 0.0005 0.0186
Vacuole 0.0005 0.0038 0.0247 0.045 0.0187 0.0039 0.0074 0.0153 0.0225 0.0251 0.0173 0.0035 0.0003 0.0008 0.0279 0.0036 0.0223 0.0007
Vacuole Periphery 0.0003 0.0007 0.003 0.0045 0.0029 0.0003 0.0024 0.0013 0.0035 0.0054 0.0039 0.0007 0.0004 0.0003 0.0047 0.0008 0.0023 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.7182 6.4321 37.6214 40.2114 22.2247 24.5347 14.5371 24.1417 35.514 26.9798
Translational Efficiency 1.3012 0.9666 1.1213 1.2639 1.1237 1.138 0.9387 0.9721 1.0172 0.9913

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages nucleus (87%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Mcm2

Mcm2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mcm2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available