Standard name | |
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Human Ortholog | |
Description | S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase; methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 0 | 1 | 0 | 0 | – | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 2 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 3 | 3 | 11 | 11 | 1 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 38 | 20 | 7 | 13 | 17 | 15 | – | 13 | 17 | 17 | 8 | 31 | 37 | 47 | 70 | 50 | 37 | 1 | 2 | 5 | 3 | 4 | 4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 5 | 3 | 3 | 0 | 0 | 1 | 0 | 1 | 0 |
Endosome | 0 | 1 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 1 | 1 | 0 | 1 | 1 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2 | 1 | 0 | 0 | 1 | 3 | 3 | – | 10 | 2 | 28 | 14 | 1 | 0 | 1 | 11 | 9 | 22 | 0 | 0 | 1 | 0 | 1 | 5 |
Nucleus | 197 | 362 | 218 | 121 | 164 | 302 | 408 | – | 402 | 427 | 365 | 339 | 274 | 259 | 286 | 492 | 373 | 280 | 204 | 389 | 239 | 158 | 225 | 198 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 1 | – | 3 | 0 | 3 | 5 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 12 | 16 | 4 | 6 | 14 | 39 | 88 | – | 102 | 116 | 148 | 98 | 13 | 24 | 19 | 24 | 15 | 10 | 1 | 6 | 4 | 6 | 20 | 36 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 4 | 6 | 10 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 6 | 12 | 2 | 6 | 3 | 4 | – | 12 | 7 | 8 | 0 | 2 | 2 | 7 | 19 | 13 | 20 | 2 | 14 | 7 | 17 | 63 | 97 |
Unique Cell Count | 207 | 406 | 252 | 132 | 190 | 341 | 488 | 486 | 509 | 477 | 413 | 312 | 307 | 354 | 578 | 441 | 338 | 213 | 423 | 268 | 191 | 328 | 356 | |
Labelled Cell Count | 217 | 425 | 256 | 136 | 200 | 365 | 521 | 548 | 574 | 583 | 476 | 327 | 331 | 372 | 625 | 469 | 373 | 213 | 423 | 268 | 191 | 328 | 356 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 25.6 | 43.2 | 27.8 | 24.1 | 22.9 | 18.2 | 17.0 | – | 14.5 | 15.0 | 12.8 | 13.2 | 29.5 | 29.2 | 28.4 | 51.2 | 49.0 | 47.8 | 26.4 | 27.8 | 27.7 |
Std Deviation (1e-4) | 8.1 | 21.4 | 10.0 | 10.2 | 7.0 | 5.7 | 5.7 | – | 4.5 | 5.5 | 5.1 | 4.7 | 13.5 | 15.1 | 15.8 | 25.5 | 22.8 | 20.9 | 10.7 | 12.0 | 11.8 |
Intensity Change (Log2) | – | – | – | -0.21 | -0.28 | -0.61 | -0.71 | – | -0.94 | -0.89 | -1.12 | -1.08 | 0.08 | 0.07 | 0.03 | 0.88 | 0.81 | 0.78 | -0.08 | -0.0 | -0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.0 | -0.4 | -1.5 | -3.0 | – | -3.3 | -2.8 | -2.6 | -3.7 | 0.8 | 1.6 | 2.1 | 1.8 | 1.4 | 1.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 3.9 | 3.0 | 0 | 0 | 0 | 0 | 0 | 4.1 |
Nucleus | 1.5 | -0.1 | 0.8 | -1.0 | – | -1.3 | -0.9 | -3.2 | -1.5 | 0.5 | -0.7 | -1.9 | -0.5 | -0.7 | -1.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 3.0 | 4.6 | 6.4 | – | 7.1 | 7.6 | 9.3 | 7.7 | 1.8 | 3.4 | 2.4 | 1.9 | 1.4 | 1.1 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | -0.8 | -3.0 | -3.5 | – | -1.7 | -2.8 | -2.4 | 0 | -3.1 | -3.1 | -1.9 | -1.0 | -1.2 | 0.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 26.0992 | 25.202 | 22.2025 | 22.7527 | 16.7921 | 25.1862 | 31.1322 | 29.5603 | 27.4621 | 25.9298 | 25.6973 | 27.7906 | 37.8546 | 33.1109 | 33.3034 | 31.5739 | 29.9708 | 32.3761 |
Actin | 0.0004 | 0 | 0 | 0 | 0.0097 | 0 | 0.0031 | 0 | 0.0015 | 0.0006 | 0.0313 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0.0002 | 0.0001 | 0.0018 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0001 | 0 | 0.0001 | 0 | 0.0013 | 0.0002 | 0.0001 | 0 | 0.0007 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0002 |
Bud Periphery | 0 | 0 | 0.0001 | 0.0001 | 0.0023 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0318 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0023 | 0.0001 | 0.0002 | 0.0001 | 0.0009 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.0012 | 0 | 0.0003 | 0 | 0.0016 | 0.002 | 0.0018 | 0.0004 | 0.0005 | 0.0003 | 0.0004 | 0.0002 | 0.0003 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.013 | 0.0095 | 0.0039 | 0.0007 | 0.0173 | 0.0028 | 0.0053 | 0.0025 | 0.0074 | 0.015 | 0.0127 | 0.006 | 0.0033 | 0 | 0 | 0 | 0 | 0 |
Eisosomes | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0011 | 0 | 0 | 0 | 0.0007 | 0.0002 | 0.0006 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.003 | 0.0001 | 0.0031 | 0 | 0.0289 | 0.015 | 0.0085 | 0 | 0.0059 | 0.0001 | 0.0095 | 0.0009 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0511 | 0.0006 | 0.0022 | 0 | 0.006 | 0.0001 | 0.0026 | 0.0014 | 0 | 0 | 0 | 0 | 0 | 0 |
Lipid Particles | 0.0029 | 0.0015 | 0.0028 | 0.0031 | 0.0345 | 0.0001 | 0.0044 | 0.0003 | 0.0022 | 0.0016 | 0.0131 | 0.0015 | 0.0006 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.0028 | 0.0011 | 0.0014 | 0.0106 | 0.0151 | 0.0008 | 0.0026 | 0.0002 | 0.0047 | 0.0075 | 0.0027 | 0.0007 | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0006 | 0.0001 |
None | 0.001 | 0 | 0.0003 | 0.0001 | 0.0005 | 0 | 0.0015 | 0.0002 | 0.0002 | 0.0014 | 0.0004 | 0.0002 | 0.0011 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0082 | 0.0012 | 0.0004 | 0.0003 | 0.001 | 0.0002 | 0.0071 | 0.0044 | 0.0024 | 0.0018 | 0.0048 | 0.0005 | 0.0002 | 0.002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Nucleolus | 0.0292 | 0.023 | 0.0212 | 0.0267 | 0.196 | 0.0633 | 0.0477 | 0.0098 | 0.0308 | 0.057 | 0.2084 | 0.0919 | 0.0313 | 0.0183 | 0.0142 | 0.0115 | 0.1533 | 0.0914 |
Nucleus | 0.9332 | 0.9601 | 0.964 | 0.9547 | 0.5713 | 0.9135 | 0.9082 | 0.9807 | 0.9287 | 0.9069 | 0.6975 | 0.892 | 0.9608 | 0.9791 | 0.9855 | 0.9884 | 0.8456 | 0.9077 |
Peroxisomes | 0.0007 | 0.002 | 0.0002 | 0.0003 | 0.0055 | 0 | 0.0008 | 0 | 0.0008 | 0.0003 | 0.0019 | 0.0012 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0015 | 0.0004 | 0.0001 | 0 | 0.0034 | 0 | 0.0035 | 0.0002 | 0.0004 | 0.0061 | 0.0006 | 0.0016 | 0.0007 | 0.0001 | 0 | 0 | 0 | 0 |
Vacuole | 0.0005 | 0.0002 | 0.0009 | 0.0008 | 0.0154 | 0.0005 | 0.0008 | 0.0006 | 0.0023 | 0.0002 | 0.0025 | 0.0005 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 |
Vacuole Periphery | 0.0006 | 0.0005 | 0.0004 | 0.0022 | 0.0082 | 0.0004 | 0.001 | 0.0003 | 0.0037 | 0.0009 | 0.01 | 0.0005 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 93.4269 | 91.6778 | 112.9066 | 79.2567 | 81.1028 | 69.7631 | 78.0145 | 72.226 | 65.1439 | 76.5895 |
Translational Efficiency | 1.4536 | 1.7251 | 1.113 | 1.5069 | 1.4283 | 1.6085 | 1.4973 | 1.3713 | 1.3645 | 1.4407 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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