Standard name
Human Ortholog
Description Protein involved in promoting high level transcription of rDNA; subunit of UAF (upstream activation factor) for RNA polymerase I
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.07 0.05 0.05 0 – 0.05 0.08 0.07 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.12 0.05 0.06 0 – 0 0 0 0 0 0 0 0 0 0.08 0.07 0 0 0 0 0 0
Bud Site 0 0 0 0.08 0 0.05 – 0.06 0.09 0.07 0.07 0.1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0.12 0.07 0.11 0.1 – 0.05 0.05 0.07 0.05 0.11 0.16 0.22 0.28 0.06 0.09 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.08 0.3 0 0 0.06 0 – 0 0 0 0 0 0.33 0.28 0.26 0.28 0.13 0.14 0.14 0.13 0.14 0.14 0.17 0.18
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.21 0.07 0.38 0.27 0.09 0.38 – 0.32 0.46 0.38 0.47 0.47 0 0 0 0 0.11 0.06 0 0 0 0 0 0
Nucleus 0.12 0.09 0.06 0 0 0 – 0.05 0.06 0.05 0.08 0 0 0.06 0.05 0.07 0.1 0.09 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.25 0.22 0.11 0.16 0.17 0.23 – 0.22 0.25 0.2 0.19 0.14 0.25 0.18 0.19 0.31 0.31 0.26 0.23 0.22 0.15 0.13 0.13 0.13
Peroxisomes 0 0 0 0.17 0.06 0.09 – 0.07 0.08 0.06 0.09 0.1 0 0.05 0.05 0 0 0 0 0 0 0 0 0
SpindlePole 0.67 0.54 0.52 0.62 0.7 0.57 – 0.63 0.57 0.58 0.56 0.48 0.58 0.53 0.55 0.76 0.73 0.73 0.53 0.46 0.5 0.6 0.56 0.49
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 1 0 1 2 1 0 3 1
Bud 5 1 23 21 18 12 – 28 38 29 31 42 6 2 0 1 2 4 4 2 0 5 8 12
Bud Neck 14 6 41 19 24 3 – 23 10 17 20 19 32 25 22 9 22 15 6 8 4 2 11 16
Bud Site 8 0 15 31 16 21 – 36 42 30 34 58 1 0 0 0 2 1 – – – – – –
Cell Periphery 0 0 0 1 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 17 23 42 30 40 39 – 30 26 31 21 62 115 137 228 18 25 26 4 15 11 8 10 14
Endoplasmic Reticulum 1 4 0 2 1 0 – 1 0 0 0 0 0 2 1 2 5 4 0 0 0 1 1 2
Endosome 42 106 1 10 21 11 – 19 10 15 6 5 239 174 214 79 37 31 86 58 54 58 112 121
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 1 2 2 0 1 2 3 3
Mitochondria 106 26 132 110 33 150 – 184 219 167 218 268 9 4 2 9 33 14 8 7 15 6 11 17
Nucleus 59 31 22 16 12 17 – 28 30 23 35 23 31 34 45 19 30 20 12 11 7 5 7 7
Nuclear Periphery 3 3 0 0 1 0 – 1 0 1 2 1 2 0 1 1 0 0 1 0 2 2 1 6
Nucleolus 124 79 39 66 62 92 – 126 119 88 86 78 178 112 161 87 89 57 138 98 58 53 88 91
Peroxisomes 1 7 4 68 22 35 – 43 38 26 40 57 30 30 38 5 4 2 2 0 2 1 3 5
SpindlePole 336 191 178 253 262 227 – 367 273 254 258 272 423 323 454 212 212 160 322 206 189 246 379 337
Vac/Vac Membrane 2 14 2 1 1 2 – 3 2 2 1 5 5 7 4 3 6 3 6 13 6 7 12 21
Unique Cell Count 501 354 345 405 374 399 580 477 438 460 566 724 611 829 280 290 220 611 450 381 410 676 681
Labelled Cell Count 718 491 499 628 513 609 889 807 683 752 890 1073 850 1171 445 469 339 611 450 381 410 676 681


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 5.2 3.3 3.3 3.9 3.1 – 3.4 2.9 3.1 2.9 3.0 4.8 5.3 5.1 6.3 6.4 6.3 4.9 5.1 5.0
Std Deviation (1e-4) 0.6 1.2 0.9 2.0 1.5 1.2 – 1.3 1.2 1.2 1.1 3.0 1.1 1.6 1.5 1.5 1.6 1.7 1.3 1.6 1.5
Intensity Change (Log2) – – – 0.01 0.24 -0.09 – 0.05 -0.2 -0.07 -0.16 -0.15 0.55 0.68 0.63 0.94 0.95 0.94 0.58 0.64 0.61


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 -5.9 -7.5 -4.1 -3.9 -2.6
Bud Neck 0 0 0 – 0 0 0 0 0 0 -4.6 -6.4 -4.0 -1.8 -2.0
Bud Site 0 0 0 – 0 0 0 0 0 0 -5.2 0 -3.5 -2.8 -2.7
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.2 -0.6 -1.0 – -3.8 -3.5 -2.4 -4.0 -0.6 1.6 3.9 5.7 -2.4 -1.5 -0.1
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 10.8 10.3 10.4 6.6 6.9
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 -16.0 -18.7 -10.4 -7.7 -8.4
Nucleus -1.5 -2.0 -1.3 – -1.0 -0.1 -0.7 0.7 -1.6 -1.5 -0.5 -0.6 0.2 1.8 1.2
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 2.9 3.4 6.1 6.1 4.5
Peroxisomes 0 0 0 – 0 0 0 0 0 0 3.0 2.9 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0.4 1.0 6.2 5.5 5.0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8295 0.966 0.7569 0.749 1.5703 0.8226 0.3714 0.0868 0.1012 0.0788 0.2629 0.1502 2.1768 2.3368 2.0927 2.4667 2.5354 2.205
Actin 0.0018 0.0001 0.0003 0 0.0066 0.0004 0.0214 0.0002 0.0019 0.0029 0.0014 0.0043 0.001 0.0001 0.0001 0 0 0.0001
Bud 0.0001 0.0001 0.0001 0.0001 0.0008 0.0001 0.0021 0.0002 0.0012 0.0001 0.0004 0.0002 0 0.0001 0 0 0.0001 0
Bud Neck 0.0033 0.0056 0.0065 0.0076 0.0032 0.0035 0.0141 0.0048 0.014 0.0098 0.0048 0.0025 0.0016 0.0028 0.0024 0.007 0.0018 0.0018
Bud Periphery 0.0002 0.0002 0.0002 0.0003 0.0012 0.0002 0.0031 0.0004 0.0022 0.0006 0.0009 0.0005 0.0001 0.0001 0.0001 0.0001 0.0001 0
Bud Site 0.0018 0.0041 0.0024 0.0015 0.0126 0.0008 0.0139 0.0067 0.0092 0.0018 0.0052 0.001 0.0005 0.0025 0.0006 0.0004 0.0006 0.0002
Cell Periphery 0.0004 0.0003 0.0003 0.0016 0.0003 0.0001 0.001 0.0006 0.0008 0.0006 0.0007 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0
Cytoplasm 0.0016 0.0049 0.0012 0.0001 0.0075 0.0008 0.0087 0.0036 0.003 0.0002 0.0189 0.0006 0.0014 0.0008 0.0001 0.0001 0.0005 0.0001
Cytoplasmic Foci 0.0569 0.0291 0.0388 0.0101 0.0275 0.0222 0.0757 0.025 0.0319 0.0163 0.027 0.0119 0.0129 0.0064 0.011 0.0037 0.0027 0.0037
Eisosomes 0.0001 0 0 0 0.0001 0 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0 0 0 0
Endoplasmic Reticulum 0.0003 0.0005 0.0004 0.0001 0.0026 0.0005 0.002 0.0004 0.0008 0.0019 0.0015 0.0004 0.0006 0.0001 0 0 0 0
Endosome 0.0201 0.0087 0.0217 0.006 0.0291 0.0136 0.0363 0.0162 0.0179 0.0135 0.0196 0.0119 0.0046 0.002 0.0019 0.0007 0.0003 0.0008
Golgi 0.0076 0.0026 0.0105 0.0016 0.03 0.004 0.0134 0.0037 0.0047 0.0107 0.0082 0.0075 0.0016 0.001 0.0012 0.0005 0.0003 0.0005
Lipid Particles 0.1679 0.0802 0.1695 0.0437 0.0411 0.0766 0.1539 0.1076 0.128 0.1155 0.0628 0.0515 0.0481 0.0303 0.0407 0.011 0.0049 0.0118
Mitochondria 0.0022 0.0003 0.0014 0.0002 0.0814 0.0123 0.0083 0.0006 0.0036 0.0147 0.0111 0.0051 0.0003 0.0001 0.0001 0.0001 0.0001 0
None 0.0043 0.0026 0.007 0.0001 0.0165 0.0003 0.0106 0.019 0.0068 0.0004 0.0013 0.0011 0.001 0.0086 0.0001 0.0004 0.01 0.0001
Nuclear Periphery 0.0015 0.0097 0.0039 0.0004 0.0326 0.0056 0.0056 0.0073 0.0071 0.0124 0.0136 0.0023 0.0043 0.0081 0.0023 0.0004 0.0013 0.0054
Nucleolus 0.3289 0.4114 0.4626 0.485 0.3167 0.2663 0.1986 0.3936 0.4116 0.4945 0.2021 0.2557 0.357 0.4459 0.4867 0.5028 0.3373 0.2727
Nucleus 0.0245 0.0558 0.036 0.0284 0.0322 0.0208 0.0333 0.0666 0.0551 0.0228 0.0969 0.0272 0.0493 0.0959 0.0499 0.0696 0.0569 0.035
Peroxisomes 0.0161 0.0032 0.0061 0.0013 0.0086 0.0063 0.0163 0.0069 0.0057 0.0031 0.0055 0.0053 0.0024 0.0013 0.0013 0.0006 0.0007 0.0005
Punctate Nuclear 0.3559 0.3754 0.227 0.3731 0.3414 0.5638 0.3712 0.318 0.2717 0.2718 0.5107 0.6085 0.5121 0.3908 0.4004 0.4013 0.5819 0.6671
Vacuole 0.0038 0.0042 0.0033 0.0378 0.0037 0.0014 0.0069 0.0172 0.0197 0.0033 0.0059 0.002 0.0009 0.0025 0.0008 0.0011 0.0002 0.0001
Vacuole Periphery 0.0006 0.0009 0.0008 0.0007 0.0043 0.0005 0.0031 0.0012 0.0031 0.003 0.0014 0.0005 0.0003 0.0002 0.0001 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.0768 9.008 15.0397 7.3556 8.1656 10.0712 10.8157 12.2945 8.0221 9.3753
Translational Efficiency 2.104 2.1328 0.93 1.8692 1.7277 2.4034 1.7709 1.7488 2.0569 2.1356

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in promoting high level transcription of rDNA; subunit of UAF (upstream activation factor) for RNA polymerase I
Localization
Cell Percentages nucleolus (27%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-1

Rrn10

Rrn10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rrn10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available