ORF | |
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Human Ortholog | |
Description | 3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud | 0 | 1 | 2 | 1 | 0 | 4 | 7 | 3 | 12 | 8 | 9 | 7 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 4 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 7 | 4 | 3 | 7 | 8 | 7 | 12 | 8 | 9 | 3 | 3 | 0 | 4 | 11 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 246 | 124 | 304 | 304 | 428 | 397 | 587 | 481 | 297 | 357 | 262 | 280 | 394 | 416 | 448 | 142 | 166 | 153 | 238 | 122 | 286 | 148 | 473 | 386 |
Endoplasmic Reticulum | 0 | 0 | 2 | 2 | 0 | 1 | 5 | 6 | 2 | 4 | 0 | 1 | 1 | 3 | 2 | 6 | 7 | 8 | 0 | 0 | 2 | 0 | 1 | 1 |
Endosome | 0 | 0 | 0 | 1 | 8 | 2 | 5 | 9 | 7 | 6 | 4 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 3 | 4 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 |
Mitochondria | 4 | 1 | 2 | 12 | 25 | 78 | 250 | 145 | 188 | 217 | 217 | 188 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 4 | 0 | 1 | 2 |
Nucleus | 1 | 1 | 0 | 0 | 1 | 2 | 4 | 7 | 3 | 7 | 5 | 4 | 0 | 1 | 2 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 2 | 2 | 1 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Vac/Vac Membrane | 1 | 0 | 2 | 3 | 14 | 13 | 43 | 52 | 44 | 56 | 66 | 34 | 0 | 0 | 0 | 2 | 3 | 4 | 2 | 1 | 2 | 1 | 4 | 2 |
Unique Cell Count | 250 | 126 | 308 | 314 | 449 | 440 | 774 | 614 | 451 | 549 | 436 | 431 | 397 | 419 | 451 | 144 | 171 | 155 | 254 | 128 | 303 | 158 | 504 | 416 |
Labelled Cell Count | 253 | 128 | 319 | 328 | 479 | 505 | 916 | 714 | 570 | 666 | 577 | 519 | 399 | 421 | 458 | 166 | 182 | 172 | 254 | 128 | 303 | 158 | 504 | 416 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.9 | 6.2 | 6.5 | 6.5 | 6.4 | 5.6 | 5.2 | 5.3 | 5.1 | 5.1 | 4.9 | 5.0 | 8.3 | 8.1 | 7.5 | 9.6 | 9.9 | 8.7 | 7.9 | 8.7 | 8.9 |
Std Deviation (1e-4) | 0.9 | 1.1 | 1.5 | 1.5 | 1.7 | 1.4 | 1.3 | 1.4 | 1.3 | 1.2 | 1.5 | 1.0 | 1.0 | 1.2 | 1.1 | 1.5 | 1.7 | 1.8 | 1.3 | 1.7 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.03 | -0.23 | -0.32 | -0.29 | -0.37 | -0.36 | -0.41 | -0.39 | 0.34 | 0.31 | 0.19 | 0.55 | 0.6 | 0.41 | 0.28 | 0.41 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.9 | 0 | -0.7 | 0 | 0 | 0.3 | -0.9 | -0.2 | 0 | 0 | 0 | 0 | 2.7 | 0 | 0 |
Cytoplasm | -1.6 | -2.6 | -4.7 | -8.9 | -8.2 | -10.9 | -11.3 | -12.2 | -11.1 | 0.7 | 0.8 | 0.9 | -0.1 | -1.3 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.7 | 3.6 | 7.4 | 11.1 | 9.0 | 12.8 | 12.5 | 14.5 | 13.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 2.3 | 2.2 | 3.6 | 4.8 | 5.2 | 5.3 | 6.8 | 4.5 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.4152 | 5.6829 | 5.1865 | 4.6145 | 4.74 | 5.2321 | 0.6092 | 2.0365 | 1.316 | 1.6821 | 0.7792 | 1.4087 | 1.2452 | 2.2396 | 1.3812 | 1.5315 | 1.1022 | 1.3309 |
Actin | 0.0003 | 0.0097 | 0.0012 | 0.0015 | 0.0004 | 0.0015 | 0.032 | 0.002 | 0.0174 | 0.0164 | 0.0197 | 0.0049 | 0.0459 | 0.0005 | 0.0117 | 0.014 | 0.003 | 0.0005 |
Bud | 0 | 0.0031 | 0.0002 | 0.0015 | 0.0001 | 0.0001 | 0.0006 | 0.0007 | 0.0008 | 0.0003 | 0.002 | 0.0008 | 0.0005 | 0.017 | 0.007 | 0.0061 | 0.0002 | 0.0003 |
Bud Neck | 0.0001 | 0.0008 | 0.0005 | 0.0006 | 0.0002 | 0.0008 | 0.0015 | 0.0014 | 0.0008 | 0.0012 | 0.0078 | 0.0013 | 0.0015 | 0.0002 | 0.0003 | 0.0202 | 0.0003 | 0.0032 |
Bud Periphery | 0 | 0.0025 | 0.0002 | 0.0024 | 0.0001 | 0.0001 | 0.0009 | 0.0009 | 0.0007 | 0.0003 | 0.0013 | 0.0008 | 0.0005 | 0.0033 | 0.0015 | 0.0027 | 0.0001 | 0.0003 |
Bud Site | 0.0002 | 0.0288 | 0.0006 | 0.0006 | 0.0001 | 0.0001 | 0.0023 | 0.0081 | 0.0092 | 0.0019 | 0.0021 | 0.0003 | 0.0033 | 0.0071 | 0.0019 | 0.0041 | 0.0006 | 0.0001 |
Cell Periphery | 0.0005 | 0.001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0006 | 0.0001 | 0.0001 |
Cytoplasm | 0.7859 | 0.7802 | 0.7467 | 0.626 | 0.7816 | 0.7904 | 0.6326 | 0.8807 | 0.8587 | 0.9372 | 0.6249 | 0.9061 | 0.6781 | 0.8898 | 0.86 | 0.8009 | 0.8202 | 0.9326 |
Cytoplasmic Foci | 0.0039 | 0.0054 | 0.0073 | 0.0262 | 0.0056 | 0.0071 | 0.0457 | 0.0194 | 0.011 | 0.0071 | 0.0106 | 0.0139 | 0.041 | 0.0147 | 0.0106 | 0.0141 | 0.0102 | 0.0141 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0003 | 0 | 0 |
Endoplasmic Reticulum | 0.0081 | 0.0031 | 0.0048 | 0.004 | 0.0037 | 0.0041 | 0.0252 | 0.0033 | 0.0025 | 0.0021 | 0.006 | 0.0027 | 0.0052 | 0.0035 | 0.0035 | 0.0018 | 0.0129 | 0.0026 |
Endosome | 0.0019 | 0.0015 | 0.0034 | 0.0136 | 0.0058 | 0.0032 | 0.0833 | 0.0161 | 0.0182 | 0.0065 | 0.06 | 0.0095 | 0.0236 | 0.0021 | 0.0069 | 0.0038 | 0.055 | 0.0122 |
Golgi | 0.0002 | 0.0004 | 0.0006 | 0.0011 | 0.0003 | 0.0003 | 0.0137 | 0.0062 | 0.007 | 0.006 | 0.0278 | 0.0052 | 0.0066 | 0.0002 | 0.0016 | 0.0025 | 0.0023 | 0.0007 |
Lipid Particles | 0.0004 | 0.0002 | 0.0003 | 0.0012 | 0.0003 | 0.0002 | 0.0189 | 0.0054 | 0.0019 | 0.0023 | 0.0167 | 0.0049 | 0.0149 | 0.0002 | 0.0026 | 0.0025 | 0.0023 | 0.0002 |
Mitochondria | 0.0001 | 0.0002 | 0.0003 | 0.0016 | 0.0002 | 0.0002 | 0.0055 | 0.004 | 0.0125 | 0.003 | 0.0197 | 0.0206 | 0.0026 | 0.0009 | 0.0094 | 0.0243 | 0.002 | 0.0009 |
None | 0.1949 | 0.156 | 0.2281 | 0.3051 | 0.1989 | 0.1882 | 0.0723 | 0.0348 | 0.044 | 0.0102 | 0.0244 | 0.0189 | 0.1359 | 0.0545 | 0.0686 | 0.0786 | 0.0229 | 0.0188 |
Nuclear Periphery | 0.0006 | 0.0007 | 0.001 | 0.0019 | 0.0006 | 0.0005 | 0.0295 | 0.0009 | 0.0012 | 0.0007 | 0.0692 | 0.0007 | 0.0085 | 0.0007 | 0.0028 | 0.0019 | 0.0452 | 0.0044 |
Nucleolus | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0005 | 0.0021 | 0.0002 | 0.0001 | 0.0039 | 0.0001 | 0.0019 | 0.0005 | 0.0016 | 0.0019 | 0.0006 | 0.001 |
Nucleus | 0.0008 | 0.0014 | 0.0013 | 0.0024 | 0.0006 | 0.0009 | 0.0049 | 0.0014 | 0.0015 | 0.001 | 0.0519 | 0.0016 | 0.0051 | 0.0016 | 0.0033 | 0.006 | 0.01 | 0.0025 |
Peroxisomes | 0 | 0.0002 | 0.0001 | 0.0013 | 0.0001 | 0.0001 | 0.0106 | 0.008 | 0.0076 | 0.0021 | 0.002 | 0.0058 | 0.0121 | 0.0003 | 0.0009 | 0.0066 | 0.0003 | 0.0001 |
Punctate Nuclear | 0.0001 | 0.0006 | 0.001 | 0.0057 | 0.0002 | 0.0009 | 0.0045 | 0.0016 | 0.0013 | 0.0004 | 0.0365 | 0.0004 | 0.0067 | 0.0012 | 0.0033 | 0.0024 | 0.0043 | 0.0007 |
Vacuole | 0.0016 | 0.0035 | 0.0017 | 0.0023 | 0.0012 | 0.0008 | 0.0095 | 0.0023 | 0.0025 | 0.001 | 0.0067 | 0.001 | 0.0047 | 0.0011 | 0.0016 | 0.0034 | 0.005 | 0.0033 |
Vacuole Periphery | 0.0001 | 0.0003 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.005 | 0.0004 | 0.0009 | 0.0003 | 0.0063 | 0.0004 | 0.0011 | 0.0002 | 0.0005 | 0.0014 | 0.0025 | 0.0017 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.5063 | 24.3259 | 18.0509 | 15.8515 | 15.3646 | 24.565 | 15.0749 | 15.2618 | 12.7185 | 21.0942 |
Translational Efficiency | 0.9281 | 0.5079 | 0.6979 | 0.6642 | 0.877 | 0.543 | 1.0318 | 0.8517 | 0.9648 | 0.7913 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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