Standard name | |
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Human Ortholog | |
Description | Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 0 | 1 | 5 | 6 | 7 | 18 | 9 | 11 | 13 | 18 | 2 | 6 | 5 | 14 | 13 | 13 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 164 | 233 | 136 | 130 | 172 | 194 | 243 | 175 | 119 | 139 | 124 | 160 | 256 | 328 | 331 | 343 | 268 | 193 | 170 | 233 | 128 | 165 | 247 | 234 |
Endoplasmic Reticulum | 0 | 3 | 0 | 0 | 2 | 4 | 7 | 11 | 6 | 9 | 8 | 10 | 1 | 2 | 1 | 12 | 7 | 7 | 0 | 0 | 2 | 0 | 0 | 6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 1 | 1 | 1 | 4 | 10 | 11 | 6 | 13 | 17 | 25 | 0 | 0 | 0 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleus | 0 | 2 | 0 | 0 | 2 | 1 | 2 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 2 | 4 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 3 | 0 | 2 | 4 | 2 | 0 | 1 | 4 | 1 | 1 | 10 | 4 | 0 | 0 | 0 | 8 | 2 | 2 | 1 | 1 | 1 | 0 | 0 | 1 |
Unique Cell Count | 167 | 236 | 139 | 133 | 178 | 203 | 263 | 202 | 130 | 160 | 160 | 200 | 257 | 331 | 333 | 355 | 273 | 200 | 178 | 240 | 141 | 172 | 252 | 253 |
Labelled Cell Count | 170 | 240 | 140 | 136 | 184 | 210 | 273 | 222 | 141 | 179 | 178 | 223 | 260 | 337 | 339 | 389 | 294 | 216 | 178 | 240 | 141 | 172 | 252 | 253 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 17.6 | 21.1 | 16.3 | 14.9 | 16.5 | 15.1 | 13.5 | 14.8 | 14.9 | 13.5 | 13.2 | 13.9 | 21.9 | 21.2 | 20.9 | 17.7 | 23.9 | 25.2 | 18.8 | 18.4 | 18.9 |
Std Deviation (1e-4) | 2.7 | 3.7 | 3.0 | 4.1 | 3.9 | 3.8 | 3.5 | 4.0 | 4.2 | 3.5 | 3.3 | 3.8 | 3.7 | 3.8 | 3.7 | 4.3 | 4.8 | 6.5 | 3.3 | 3.7 | 4.2 |
Intensity Change (Log2) | – | – | – | -0.13 | 0.02 | -0.11 | -0.27 | -0.14 | -0.14 | -0.28 | -0.31 | -0.24 | 0.42 | 0.38 | 0.36 | 0.12 | 0.55 | 0.62 | 0.2 | 0.17 | 0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 3.6 | 0 | 3.1 | 3.4 | 3.6 | 0 | 0 | 0 | 0 | 0 | 3.1 |
Cytoplasm | -0.1 | -0.6 | -1.1 | -2.2 | -3.6 | -2.3 | -3.5 | -5.2 | -4.9 | 1.7 | 1.1 | 1.5 | -0.7 | 0.2 | -0.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 3.6 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 17.1385 | 22.2494 | 20.4479 | 19.2885 | 20.0951 | 21.2601 | 14.6245 | 20.8145 | 18.7628 | 15.6756 | 16.9326 | 18.5436 | 18.577 | 24.3249 | 22.3597 | 23.3549 | 22.6007 | 22.6282 |
Actin | 0.0291 | 0.0029 | 0.0142 | 0.0283 | 0.0002 | 0.0001 | 0.0246 | 0.0004 | 0.0012 | 0.0336 | 0.0007 | 0.0002 | 0.0216 | 0.0003 | 0.0017 | 0.0001 | 0.0028 | 0.0004 |
Bud | 0.0003 | 0.0001 | 0.0004 | 0.0004 | 0.0001 | 0.0002 | 0.0009 | 0.0001 | 0.0002 | 0.0013 | 0.0001 | 0 | 0.0015 | 0.0003 | 0.0003 | 0 | 0.0001 | 0.0001 |
Bud Neck | 0.0009 | 0.0002 | 0.0028 | 0.0007 | 0.0009 | 0.0009 | 0.0007 | 0.0001 | 0.0003 | 0.0006 | 0.0006 | 0.0006 | 0.0022 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0006 |
Bud Periphery | 0.0003 | 0 | 0.0004 | 0.0008 | 0 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0007 | 0 | 0 | 0.0009 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Site | 0.0004 | 0.0001 | 0.0018 | 0.0008 | 0 | 0 | 0.0053 | 0.0001 | 0.0002 | 0.0013 | 0.0001 | 0.0001 | 0.002 | 0.0002 | 0.0004 | 0 | 0.0002 | 0 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0.0005 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.8396 | 0.9761 | 0.9176 | 0.8601 | 0.9455 | 0.9208 | 0.7673 | 0.9932 | 0.9175 | 0.8496 | 0.9309 | 0.9569 | 0.8147 | 0.9907 | 0.9472 | 0.9868 | 0.9007 | 0.9568 |
Cytoplasmic Foci | 0.0146 | 0.0005 | 0.0064 | 0.0031 | 0.0011 | 0.0004 | 0.0237 | 0.0005 | 0.0142 | 0.0288 | 0.024 | 0.005 | 0.0361 | 0.0006 | 0.0057 | 0.0003 | 0.0091 | 0.0012 |
Eisosomes | 0.0002 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.008 | 0.0006 | 0.0017 | 0.003 | 0.0054 | 0.0027 | 0.0278 | 0.0005 | 0.0022 | 0.0034 | 0.0083 | 0.0013 | 0.0086 | 0.0002 | 0.0004 | 0.0003 | 0.0217 | 0.0009 |
Endosome | 0.0224 | 0.0005 | 0.0084 | 0.0132 | 0.0011 | 0.0007 | 0.0565 | 0.0001 | 0.0128 | 0.0355 | 0.0084 | 0.0076 | 0.037 | 0.0001 | 0.0007 | 0.0001 | 0.0339 | 0.0008 |
Golgi | 0.0049 | 0.0017 | 0.003 | 0.0071 | 0 | 0 | 0.0188 | 0 | 0.0039 | 0.0127 | 0.0007 | 0.0009 | 0.0061 | 0 | 0.0007 | 0 | 0.0025 | 0.0001 |
Lipid Particles | 0.007 | 0.0005 | 0.007 | 0.0089 | 0 | 0 | 0.0127 | 0 | 0.0112 | 0.0073 | 0.0011 | 0.0087 | 0.0127 | 0 | 0.0014 | 0 | 0.0052 | 0.0001 |
Mitochondria | 0.0026 | 0.0008 | 0.0078 | 0.0163 | 0.0002 | 0.0002 | 0.0092 | 0.0001 | 0.0008 | 0.0076 | 0.0002 | 0.0003 | 0.0029 | 0.0001 | 0.0147 | 0.0001 | 0.0007 | 0.0081 |
None | 0.0034 | 0.0002 | 0.0014 | 0.0006 | 0.001 | 0.0002 | 0.0042 | 0.0005 | 0.0015 | 0.0012 | 0.0031 | 0.0006 | 0.0062 | 0.0003 | 0.0007 | 0.0002 | 0.0048 | 0.0005 |
Nuclear Periphery | 0.0198 | 0.0018 | 0.0043 | 0.0067 | 0.0116 | 0.0096 | 0.0134 | 0.0006 | 0.0041 | 0.0019 | 0.0028 | 0.002 | 0.0083 | 0.0006 | 0.0018 | 0.0017 | 0.0081 | 0.0027 |
Nucleolus | 0.0003 | 0 | 0.0002 | 0.0006 | 0 | 0 | 0.0004 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0.0001 | 0 |
Nucleus | 0.033 | 0.0131 | 0.0157 | 0.0249 | 0.0297 | 0.0614 | 0.0118 | 0.0036 | 0.0231 | 0.0065 | 0.0166 | 0.014 | 0.0238 | 0.006 | 0.0139 | 0.0099 | 0.006 | 0.0268 |
Peroxisomes | 0.0042 | 0.0003 | 0.0036 | 0.0034 | 0 | 0 | 0.0046 | 0 | 0.0014 | 0.0034 | 0.0006 | 0.0008 | 0.0043 | 0 | 0.0086 | 0 | 0.0002 | 0.0001 |
Punctate Nuclear | 0.0051 | 0.0001 | 0.0016 | 0.0007 | 0.0008 | 0.0003 | 0.0055 | 0.0001 | 0.0036 | 0.0011 | 0.0012 | 0.0004 | 0.0066 | 0.0001 | 0.0012 | 0.0001 | 0.0021 | 0.0005 |
Vacuole | 0.0022 | 0.0004 | 0.001 | 0.0137 | 0.0018 | 0.0017 | 0.0087 | 0.0001 | 0.0011 | 0.0022 | 0.0006 | 0.0004 | 0.0027 | 0.0001 | 0.0002 | 0.0001 | 0.0009 | 0.0003 |
Vacuole Periphery | 0.0014 | 0.0001 | 0.0006 | 0.0062 | 0.0005 | 0.0005 | 0.0025 | 0 | 0.0006 | 0.001 | 0.0001 | 0.0001 | 0.0009 | 0 | 0.0001 | 0 | 0.0006 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 100.3808 | 107.2605 | 79.0717 | 93.2833 | 95.5775 | 90.9197 | 115.3621 | 105.2508 | 110.1298 | 106.0678 |
Translational Efficiency | 0.686 | 0.6759 | 0.8326 | 0.7871 | 0.6672 | 0.7437 | 0.7653 | 0.7355 | 0.6829 | 0.5955 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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