Standard name
Human Ortholog
Description UBX domain-containing substrate adaptor for Cdc48p; ubiquitin regulatory X domain-containing protein that acts as a substrate recruiting cofactor for Cdc48p; positively regulates Glc7p PPase activity to promote growth and mitotic progression in complex with Cdc48p; ubiquitinated protein interactor involved in ER-associated degradation (ERAD); regulated by nuclear Ub-dependent degradation (INMAD pathway) independent of the Asi and Doa10 complexes; homolog of human p47 (NSFL1C)

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0.58 0.3 0.3 0.23 0.47 0.33 0.31 0.4 0.35 0.37 0.41 0.58 0.48 0.53 0.44 0.4 0.46 0.38 0.28 0.32 0.28 0.21 0.16
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.15 0.08 0.09 0.19 0.21 0.26 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.07 0 0.09 0.07 0.06 0.07 0.06 0.05 0.11 0.08 0.09 0.11 0.17 0.08 0.21 0.1 0.06 0.08 0.42 0.48 0.33 0.5 0.56 0.55
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.78 0.57 0.7 0.7 0.75 0.54 0.53 0.61 0.5 0.48 0.48 0.34 0.41 0.59 0.46 0.59 0.66 0.59 0.09 0.14 0.17 0.13 0.1 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 0 0 0 1 8 6 5 10 6 0 0 0 0 0 0 0 3 4 3 7 13 17
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 4
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 1 3 2 5 1 3 0 0 1 2 0 1 0 0 0 0 0 0
Cytoplasm 66 57 87 66 79 189 168 162 178 196 75 105 142 163 190 114 100 114 152 32 103 60 63 55
Endoplasmic Reticulum 1 0 0 0 0 1 6 3 6 11 1 4 3 6 3 10 7 8 6 0 8 0 2 2
Endosome 10 0 4 3 5 1 6 1 2 0 0 0 0 5 0 0 1 0 7 1 9 3 2 8
Golgi 0 0 2 2 2 0 0 0 1 0 0 0 0 1 5 13 5 5 0 0 2 0 1 1
Mitochondria 2 1 3 5 5 25 77 44 41 105 42 66 0 0 2 2 4 0 1 0 4 0 4 1
Nucleus 24 3 25 15 22 28 31 28 48 45 18 27 41 28 75 25 14 20 165 55 109 107 172 192
Nuclear Periphery 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 5 0 10 0 3 4
Nucleolus 2 0 1 0 1 0 3 3 1 3 1 2 0 0 0 0 0 0 3 0 4 0 1 5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 6 3 2 7
Vac/Vac Membrane 259 56 203 153 258 217 271 324 220 273 96 87 100 199 165 151 165 146 37 16 54 27 32 45
Unique Cell Count 333 98 289 218 342 403 507 529 440 563 201 255 245 338 358 257 251 246 399 116 327 215 308 353
Labelled Cell Count 365 117 325 244 372 464 572 574 504 649 241 294 286 402 441 317 296 294 399 116 327 215 308 353


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.4 12.7 12.2 12.0 12.6 11.6 10.9 10.8 10.5 10.0 9.3 9.3 14.0 14.8 14.3 16.2 15.0 16.1 12.7 13.5 14.9
Std Deviation (1e-4) 1.7 2.0 2.4 2.2 2.4 2.4 2.5 2.4 2.4 2.3 2.6 2.4 2.6 3.0 3.2 4.3 3.1 3.9 3.2 2.9 3.1
Intensity Change (Log2) -0.03 0.04 -0.08 -0.17 -0.18 -0.22 -0.3 -0.39 -0.4 0.2 0.28 0.23 0.41 0.29 0.4 0.05 0.14 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -2.0 4.4 0.9 0.2 2.8 1.4 1.7 2.7 6.5 4.6 5.9 3.4 2.4 3.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 3.1 0 0
Mitochondria 0 0 3.4 6.4 4.3 4.6 7.3 7.5 8.7 0 0 0 0 0 0
Nucleus -0.7 -1.1 -0.8 -1.3 -1.9 1.0 -0.3 0.1 0.8 2.8 -0.2 4.3 0.4 -1.4 -0.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.5 -4.4 -4.6 -2.6 -5.4 -6.1 -5.0 -8.4 -6.8 -3.0 -6.2 -2.8 -1.1 -2.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.0639 0.0002 0.0039 0.0053 0.0409 0.0017 0.0021 0.0001 0.0043 0.0047 0.0001 0.0001
Bud 0.0005 0.0001 0.0006 0.0002 0.0019 0.0001 0.0005 0 0.0003 0.0003 0 0.0001
Bud Neck 0.001 0.0002 0.0011 0.0005 0.0005 0.0006 0.002 0.0001 0.0005 0.001 0.0006 0.0011
Bud Periphery 0.0006 0 0.0012 0.0002 0.0022 0 0.0003 0 0.0002 0.0002 0 0
Bud Site 0.0016 0.0002 0.0006 0.0005 0.0033 0 0.0137 0.0001 0.0019 0.0006 0.0001 0.0001
Cell Periphery 0.0001 0 0.0007 0.0001 0.0001 0 0.0002 0 0.0001 0.0001 0 0
Cytoplasm 0.0285 0.072 0.0492 0.0496 0.1182 0.0513 0.0803 0.0936 0.0655 0.0989 0.0835 0.0786
Cytoplasmic Foci 0.0326 0.0001 0.0049 0.0084 0.0071 0.0026 0.0117 0.0002 0.0084 0.0136 0.0001 0.0078
Eisosomes 0.0004 0 0 0 0.0002 0 0 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0086 0.0003 0.0033 0.0012 0.0011 0.0003 0.0063 0.0006 0.0031 0.0059 0.0005 0.0013
Endosome 0.0381 0.0001 0.0132 0.0085 0.0079 0.0062 0.0141 0.0004 0.0196 0.0158 0.0002 0.0142
Golgi 0.0148 0 0.0034 0.0165 0.016 0.0031 0.0012 0 0.0042 0.0022 0 0.0006
Lipid Particles 0.0169 0 0.0033 0.0071 0.0101 0.002 0.0022 0 0.0073 0.007 0 0.0036
Mitochondria 0.0079 0.0004 0.0033 0.0105 0.054 0.0011 0.0013 0.0001 0.0078 0.0019 0.0001 0.0004
None 0.0159 0.0001 0.0004 0.0015 0.0006 0.0001 0.0177 0.0012 0.0016 0.0428 0.0001 0.0037
Nuclear Periphery 0.0522 0.0189 0.0226 0.0191 0.0212 0.0238 0.04 0.0194 0.0266 0.03 0.0317 0.023
Nucleolus 0.0023 0.0012 0.001 0.0006 0.001 0.0006 0.0039 0.0005 0.0011 0.003 0.0006 0.0012
Nucleus 0.6758 0.9045 0.8807 0.8633 0.7068 0.8994 0.7777 0.8807 0.8323 0.7442 0.8773 0.8515
Peroxisomes 0.0118 0 0.0019 0.0022 0.0034 0.0046 0.0008 0 0.0026 0.0009 0 0.0006
Punctate Nuclear 0.0179 0.001 0.0013 0.0035 0.0011 0.0016 0.0167 0.0021 0.0092 0.0203 0.0045 0.0062
Vacuole 0.0054 0.0003 0.0027 0.0007 0.001 0.0005 0.0057 0.0005 0.0027 0.0049 0.0004 0.0047
Vacuole Periphery 0.0032 0.0002 0.0007 0.0004 0.0016 0.0003 0.0016 0.0001 0.0008 0.0018 0.0001 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 81.9746 79.0962 90.9163 97.6504 73.6675 79.7618 93.1071 109.2772 115.3267 77.1098
Translational Efficiency 2.4463 2.1719 2.0624 2.2436 2.3986 2.7125 1.7583 1.8881 1.9087 2.0808

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description UBX domain-containing substrate adaptor for Cdc48p; ubiquitin regulatory X domain-containing protein that acts as a substrate recruiting cofactor for Cdc48p; positively regulates Glc7p PPase activity to promote growth and mitotic progression in complex with Cdc48p; ubiquitinated protein interactor involved in ER-associated degradation (ERAD); regulated by nuclear Ub-dependent degradation (INMAD pathway) independent of the Asi and Doa10 complexes; homolog of human p47 (NSFL1C)
Localization
Cell Percentages cytoplasm (43%), nucleus (2%), mixed (54%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Shp1

Shp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Shp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available