Standard name
Human Ortholog
Description Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.06 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.11 0.1 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0.09 0.17 0.07 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.76 0.79 0.62 0.67 0.52 0.71 0.73 0.62 0.62 0.54 0.49 0.52 0.86 0.91 0.83 0.94 0.92 0.91 0.47 0.49 0.43 0.25 0.35 0.22
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0.05 0.14 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0.12 0.14 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.08 0.14 0.21 0.46 0.41 0.41 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0.08 0 0.08 0.12 0.07 0 0 0 0 0 0 0.12 0.11 0.07 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Vac/Vac Membrane 0.34 0.13 0.28 0.26 0.42 0.18 0.2 0.2 0.16 0.12 0.09 0.07 0.08 0.09 0.19 0 0.05 0.1 0.15 0.14 0.12 0.09 0.27 0.54
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 12 15 5 4 2 2 0 2 0 0 0 1 6 2 2 9 6 4 3 9 10 41 33 11
Bud 5 5 4 1 4 6 6 7 8 8 12 8 1 2 0 1 0 0 19 19 31 35 20 16
Bud Neck 0 0 1 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0 23 28 24 61 36 10
Bud Site 3 4 9 5 5 9 5 6 4 3 3 6 2 0 0 3 4 2 – – – – – –
Cell Periphery 0 0 0 4 0 1 1 0 0 0 2 1 0 0 0 3 0 1 0 0 0 1 1 0
Cytoplasm 236 229 162 169 160 260 314 297 178 231 149 180 137 209 327 266 237 157 154 158 121 87 185 122
Endoplasmic Reticulum 2 4 1 5 0 1 0 2 0 0 1 2 4 3 2 9 13 3 0 0 3 14 18 11
Endosome 4 0 11 13 42 25 18 15 9 2 1 4 0 0 3 3 0 0 15 13 16 42 75 55
Golgi 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 5 0 0 1 0 2
Mitochondria 1 13 3 4 2 13 34 67 59 197 125 143 0 0 0 5 9 2 2 3 1 10 6 3
Nucleus 5 8 9 5 6 14 18 38 9 33 36 25 6 7 4 0 2 5 40 35 20 4 1 4
Nuclear Periphery 0 2 0 0 0 0 0 0 0 1 0 1 0 0 0 1 0 0 2 0 1 2 4 4
Nucleolus 0 0 0 2 2 2 2 4 3 0 3 3 0 0 0 0 0 0 0 0 0 1 1 1
Peroxisomes 4 0 13 5 15 6 3 4 6 1 0 3 0 0 0 3 1 1 0 0 0 0 0 0
SpindlePole 2 3 10 5 8 5 5 7 6 4 1 4 3 1 1 3 4 1 9 6 14 10 3 2
Vac/Vac Membrane 104 39 72 66 129 66 87 94 47 52 27 25 12 21 75 5 14 18 48 45 33 33 142 297
Unique Cell Count 309 290 261 253 310 367 430 478 287 428 302 347 159 230 396 282 258 173 329 325 285 355 537 551
Labelled Cell Count 380 322 300 289 375 410 493 543 330 533 360 406 171 245 414 312 290 194 329 325 285 355 537 551


Bud Neck, Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 5.6 6.5 6.5 6.6 5.3 5.0 4.7 4.5 4.0 4.2 4.2 6.4 6.8 6.9 5.6 6.1 7.7 8.8 8.8 9.0
Std Deviation (1e-4) 0.9 0.8 1.4 1.5 1.6 1.3 1.2 1.0 0.9 0.7 1.1 0.9 0.6 0.8 0.9 1.3 1.1 1.8 1.5 1.5 1.5
Intensity Change (Log2) – – – -0.01 0.01 -0.3 -0.38 -0.46 -0.54 -0.71 -0.64 -0.62 -0.03 0.05 0.08 -0.23 -0.11 0.24 0.43 0.43 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.9 0.3 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site -1.0 -1.4 -0.7 -2.1 0 0 0 0 0 0 0 0 -1.9 -1.4 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.1 -2.5 2.3 3.0 0 0 -2.1 -3.0 -2.5 5.3 7.4 5.9 9.2 8.0 6.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.3 0
Endosome 0.5 3.8 1.4 0 0 0 0 0 0 0 -3.1 -3.0 -2.3 -3.3 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 1.9 3.8 0 0 0 0 0 0 0 0 0 1.8 0
Nucleus -1.0 -1.1 0.2 0.5 2.4 -0.2 2.3 3.7 2.0 0.2 -0.3 -2.2 0 -2.1 -0.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -1.9 -0.1 -2.4 -3.6 0 0 0 0 0 0 -3.4 -4.5 -2.7 -3.2 -2.5
SpindlePole -1.2 -0.9 -2.0 -2.3 0 0 0 0 0 0 -2.5 0 -2.1 -1.6 0
Vacuole -0.4 3.5 -2.9 -2.2 -2.5 -3.2 -5.1 -5.8 -6.8 -5.0 -5.2 -2.6 -8.6 -6.8 -4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – 0.0103 0.0118 0.0127 0.0107 0.0011 0.0127 0.0106 0.0032 0.0027 0.0043 0.001 0.0034
Bud – – – – – – 0.0048 0.0028 0.0037 0.0007 0.0003 0.0006 0.0065 0.0049 0.0009 0.0047 0.0044 0.0008
Bud Neck – – – – – – 0.0669 0.0172 0.0127 0.0152 0.0374 0.4031 0.0256 0.0237 0.0208 0.006 0.0123 0.4682
Bud Periphery – – – – – – 0.0041 0.001 0.0024 0.0004 0.0002 0.0006 0.0049 0.0029 0.0006 0.005 0.003 0.0007
Bud Site – – – – – – 0.0727 0.1423 0.031 0.0101 0.003 0.0022 0.097 0.1548 0.0331 0.012 0.012 0.0046
Cell Periphery – – – – – – 0.0008 0.0004 0.0004 0.0004 0.0001 0.0003 0.0008 0.0006 0.0002 0.0001 0.0001 0.0002
Cytoplasm – – – – – – 0.6509 0.5553 0.6108 0.3418 0.4554 0.4243 0.6703 0.5693 0.6042 0.523 0.4995 0.3951
Cytoplasmic Foci – – – – – – 0.0299 0.0084 0.0209 0.0156 0.0064 0.0172 0.0153 0.0093 0.0059 0.0129 0.0083 0.0206
Eisosomes – – – – – – 0.0001 0 0.0001 0.0002 0 0.0001 0.0001 0 0 0 0 0
Endoplasmic Reticulum – – – – – – 0.0131 0.0043 0.0067 0.0025 0.004 0.0039 0.0074 0.0049 0.0048 0.0035 0.0045 0.0025
Endosome – – – – – – 0.0224 0.0023 0.0087 0.0052 0.0029 0.0025 0.0035 0.0057 0.0023 0.0121 0.0045 0.0032
Golgi – – – – – – 0.0058 0.0005 0.0037 0.0019 0.0008 0.0023 0.001 0.0005 0.0003 0.0004 0.0003 0.0007
Lipid Particles – – – – – – 0.0054 0.0002 0.0038 0.0043 0.0011 0.0023 0.0005 0.0003 0.0001 0.0002 0.0002 0.0003
Mitochondria – – – – – – 0.005 0.0003 0.009 0.0007 0.0005 0.0021 0.0006 0.0003 0.0004 0.0006 0.0005 0.0002
None – – – – – – 0.047 0.0062 0.0317 0.021 0.0583 0.0373 0.0539 0.0201 0.0393 0.04 0.0592 0.018
Nuclear Periphery – – – – – – 0.0065 0.0074 0.012 0.0101 0.009 0.0024 0.0066 0.0045 0.008 0.009 0.0109 0.0021
Nucleolus – – – – – – 0.0005 0.0007 0.0009 0.0028 0.001 0.0013 0.0006 0.0013 0.0009 0.0014 0.0012 0.0005
Nucleus – – – – – – 0.0292 0.2253 0.1972 0.5196 0.4068 0.0686 0.0749 0.1786 0.2579 0.3317 0.3364 0.0557
Peroxisomes – – – – – – 0.0033 0.0009 0.0068 0.0035 0.0025 0.0046 0.0034 0.0008 0.0005 0.0028 0.0003 0.0019
Punctate Nuclear – – – – – – 0.0147 0.0105 0.0224 0.0314 0.0076 0.0106 0.0133 0.0129 0.0154 0.024 0.0389 0.0206
Vacuole – – – – – – 0.0054 0.0022 0.002 0.0017 0.0014 0.0012 0.0032 0.0013 0.0014 0.0053 0.0023 0.0008
Vacuole Periphery – – – – – – 0.0013 0.0001 0.0004 0.0002 0.0002 0.0001 0.0003 0.0002 0.0002 0.0008 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 34.3 20.2842 19.2522 31.5266 34.2088 22.6782 24.0865 18.1883 23.1052 38.0984
Translational Efficiency 1.1633 1.0178 1.0577 0.6403 0.9249 1.6601 0.8567 0.8439 0.8277 0.9583

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (28%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Skt5

Skt5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Skt5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available