Standard name | |
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Human Ortholog | |
Description | Kinesin-related motor protein; required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 2 |
Bud | 0 | 0 | 4 | 0 | 4 | 5 | 6 | 21 | 24 | 12 | 17 | 22 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 6 | 4 | 6 | 4 | 7 |
Bud Neck | 0 | 3 | 16 | 6 | 12 | 5 | 7 | 8 | 11 | 5 | 6 | 12 | 2 | 2 | 1 | 1 | 0 | 2 | 1 | 2 | 0 | 3 | 0 | 3 |
Bud Site | 0 | 3 | 9 | 6 | 15 | 8 | 12 | 37 | 23 | 23 | 14 | 25 | 1 | 0 | 3 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 24 | 86 | 73 | 62 | 47 | 24 | 34 | 67 | 38 | 31 | 22 | 37 | 32 | 63 | 73 | 23 | 16 | 11 | 6 | 39 | 24 | 19 | 22 | 26 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 4 | 5 | 0 | 0 | 1 | 0 | 2 | 0 |
Endosome | 39 | 54 | 3 | 1 | 16 | 2 | 2 | 7 | 3 | 0 | 2 | 1 | 27 | 31 | 22 | 27 | 33 | 19 | 18 | 36 | 22 | 18 | 27 | 19 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 0 | 2 | 1 | 1 | 2 | 1 | 0 |
Mitochondria | 2 | 49 | 56 | 89 | 77 | 82 | 138 | 169 | 161 | 150 | 158 | 219 | 8 | 7 | 16 | 11 | 9 | 15 | 5 | 17 | 21 | 5 | 7 | 17 |
Nucleus | 4 | 17 | 3 | 20 | 36 | 21 | 28 | 46 | 31 | 38 | 24 | 37 | 2 | 4 | 2 | 17 | 7 | 16 | 3 | 16 | 1 | 2 | 3 | 2 |
Nuclear Periphery | 0 | 3 | 0 | 1 | 0 | 4 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 |
Nucleolus | 20 | 37 | 11 | 24 | 50 | 23 | 42 | 53 | 33 | 35 | 29 | 34 | 26 | 39 | 56 | 48 | 42 | 46 | 4 | 23 | 11 | 9 | 11 | 6 |
Peroxisomes | 4 | 4 | 0 | 0 | 2 | 0 | 0 | 4 | 7 | 3 | 1 | 0 | 6 | 8 | 3 | 2 | 3 | 2 | 0 | 0 | 1 | 0 | 0 | 1 |
SpindlePole | 57 | 123 | 111 | 71 | 165 | 46 | 62 | 138 | 83 | 90 | 75 | 114 | 70 | 85 | 69 | 70 | 64 | 66 | 79 | 206 | 166 | 79 | 94 | 113 |
Vac/Vac Membrane | 6 | 28 | 0 | 1 | 4 | 2 | 2 | 1 | 1 | 1 | 0 | 5 | 5 | 3 | 7 | 13 | 6 | 7 | 0 | 14 | 5 | 8 | 8 | 9 |
Unique Cell Count | 113 | 325 | 239 | 207 | 309 | 153 | 236 | 358 | 271 | 245 | 234 | 331 | 140 | 183 | 187 | 147 | 116 | 125 | 138 | 396 | 288 | 167 | 200 | 229 |
Labelled Cell Count | 156 | 407 | 286 | 281 | 428 | 222 | 333 | 551 | 415 | 388 | 350 | 508 | 180 | 242 | 252 | 220 | 187 | 190 | 138 | 396 | 288 | 167 | 200 | 229 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 3.7 | 3.3 | 3.3 | 3.6 | 3.3 | 4.0 | 3.2 | 4.5 | 2.7 | 2.8 | 2.7 | 4.7 | 4.6 | 4.1 | 6.5 | 7.4 | 7.3 | 4.6 | 4.6 | 4.9 |
Std Deviation (1e-4) | 0.8 | 0.7 | 0.6 | 1.1 | 1.2 | 1.3 | 9.9 | 1.1 | 11.3 | 1.0 | 0.9 | 3.9 | 2.0 | 1.1 | 1.3 | 1.6 | 2.3 | 2.4 | 1.9 | 1.7 | 1.2 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.12 | -0.01 | 0.29 | -0.06 | 0.45 | -0.29 | -0.27 | -0.28 | 0.5 | 0.46 | 0.32 | 0.96 | 1.16 | 1.13 | 0.48 | 0.48 | 0.57 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.7863 | 0.3556 | 0.6647 | 0.3434 | 0.8822 | 0.3311 | -2.1465 | -0.8623 | -1.0161 | -1.2733 | -1.433 | -1.1859 | 1.9261 | 3.6417 | 3.1692 | 2.6417 | 3.0932 | 2.6394 |
Actin | 0.1096 | 0.0002 | 0.017 | 0.0084 | 0.0107 | 0.0019 | 0.0027 | 0.0001 | 0 | 0.0002 | 0.0005 | 0.0034 | 0.0006 | 0.0001 | 0.0069 | 0.0018 | 0.0006 | 0.0017 |
Bud | 0.0037 | 0.0014 | 0.0003 | 0.0002 | 0.0009 | 0.0002 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 |
Bud Neck | 0.0193 | 0.0062 | 0.001 | 0.0185 | 0.0199 | 0.0303 | 0.0017 | 0.0016 | 0.0008 | 0.002 | 0.0065 | 0.0075 | 0.001 | 0.0008 | 0.0011 | 0.0013 | 0.0013 | 0.0068 |
Bud Periphery | 0.0043 | 0.0017 | 0.0005 | 0.0002 | 0.0015 | 0.0004 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0012 | 0.0001 | 0.0002 |
Bud Site | 0.0238 | 0.0123 | 0.0092 | 0.0017 | 0.0067 | 0.0008 | 0.0008 | 0.003 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0008 | 0.0018 | 0.0007 | 0.0011 | 0.0003 | 0.0007 |
Cell Periphery | 0.0006 | 0.0002 | 0.0002 | 0 | 0.0004 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0007 |
Cytoplasm | 0.0263 | 0.0388 | 0.0007 | 0.0003 | 0.0079 | 0.0286 | 0.0149 | 0.0081 | 0.0004 | 0.001 | 0.0067 | 0.0037 | 0.0321 | 0.0156 | 0.0032 | 0.0097 | 0.0461 | 0.014 |
Cytoplasmic Foci | 0.0746 | 0.0507 | 0.0288 | 0.0511 | 0.0288 | 0.0485 | 0.0415 | 0.0062 | 0.0054 | 0.0033 | 0.0275 | 0.0213 | 0.0567 | 0.0093 | 0.0562 | 0.028 | 0.0073 | 0.0412 |
Eisosomes | 0.0013 | 0 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0005 |
Endoplasmic Reticulum | 0.0074 | 0.0013 | 0.0012 | 0 | 0.0032 | 0.0012 | 0.0009 | 0.0011 | 0 | 0.0001 | 0.0002 | 0.0006 | 0.001 | 0.0009 | 0.0004 | 0.0003 | 0.0011 | 0.0008 |
Endosome | 0.0356 | 0.0071 | 0.0057 | 0.0027 | 0.0218 | 0.0128 | 0.0089 | 0.0035 | 0.0007 | 0.0005 | 0.0024 | 0.0043 | 0.0089 | 0.0029 | 0.0111 | 0.005 | 0.0066 | 0.012 |
Golgi | 0.0134 | 0.0008 | 0.007 | 0.0043 | 0.0112 | 0.0021 | 0.0012 | 0.0003 | 0.0002 | 0.0002 | 0.0005 | 0.0009 | 0.0043 | 0.0002 | 0.0153 | 0.0052 | 0.0007 | 0.0068 |
Lipid Particles | 0.047 | 0.0125 | 0.017 | 0.0158 | 0.035 | 0.0165 | 0.0175 | 0.0019 | 0.0029 | 0.0014 | 0.0082 | 0.0201 | 0.0124 | 0.0014 | 0.0205 | 0.1221 | 0.018 | 0.0196 |
Mitochondria | 0.0069 | 0.0002 | 0.0052 | 0.0368 | 0.0128 | 0.0021 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.001 | 0.0165 | 0.0002 | 0.0009 | 0.0136 | 0.0005 | 0.0036 |
None | 0.0376 | 0.1192 | 0.0008 | 0.0002 | 0.0636 | 0.0465 | 0.0267 | 0.0184 | 0.0004 | 0.0043 | 0.0055 | 0.0044 | 0.1454 | 0.175 | 0.02 | 0.0034 | 0.1585 | 0.1358 |
Nuclear Periphery | 0.024 | 0.004 | 0.0104 | 0.0012 | 0.0478 | 0.0206 | 0.0171 | 0.0189 | 0.0019 | 0.0037 | 0.0161 | 0.0116 | 0.0024 | 0.0099 | 0.0076 | 0.0116 | 0.0647 | 0.0063 |
Nucleolus | 0.0079 | 0.0463 | 0.0076 | 0.0062 | 0.0468 | 0.0441 | 0.0266 | 0.0827 | 0.0564 | 0.0287 | 0.0554 | 0.0657 | 0.0103 | 0.0174 | 0.0326 | 0.0456 | 0.0576 | 0.0394 |
Nucleus | 0.0432 | 0.0648 | 0.0171 | 0.0043 | 0.1861 | 0.1281 | 0.1248 | 0.1917 | 0.0418 | 0.0413 | 0.0904 | 0.186 | 0.0368 | 0.1746 | 0.0062 | 0.16 | 0.1343 | 0.1591 |
Peroxisomes | 0.0108 | 0.0017 | 0.0051 | 0.0286 | 0.0192 | 0.0046 | 0.0031 | 0.0002 | 0.0001 | 0.0005 | 0.0006 | 0.0034 | 0.0012 | 0.0002 | 0.0098 | 0.0135 | 0.001 | 0.0058 |
Punctate Nuclear | 0.4944 | 0.6291 | 0.8634 | 0.8189 | 0.4631 | 0.6077 | 0.709 | 0.6609 | 0.8885 | 0.9124 | 0.7785 | 0.6644 | 0.6678 | 0.5888 | 0.8065 | 0.5733 | 0.4992 | 0.543 |
Vacuole | 0.0055 | 0.0013 | 0.0006 | 0.0001 | 0.0058 | 0.0015 | 0.0016 | 0.0007 | 0.0001 | 0.0001 | 0.0003 | 0.0006 | 0.001 | 0.0006 | 0.0004 | 0.0009 | 0.0008 | 0.0017 |
Vacuole Periphery | 0.0028 | 0.0001 | 0.0012 | 0.0001 | 0.0066 | 0.0012 | 0.0004 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0015 | 0.0011 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.4188 | 10.053 | 3.7299 | 5.8837 | 9.4345 | 9.9172 | 13.4531 | 12.7119 | 6.4775 | 12.8016 |
Translational Efficiency | 0.6849 | 0.8034 | 0.6502 | 0.3452 | 0.5654 | 0.6828 | 0.4629 | 0.3542 | 0.5659 | 0.4132 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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