Standard name | |
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Human Ortholog | |
Description | Ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 3 | 4 | 1 | 4 | 6 |
Bud | 2 | 0 | 0 | 1 | 0 | 0 | 3 | – | 1 | 4 | 12 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 3 | 0 | 8 | 7 |
Bud Neck | 1 | 1 | 13 | 10 | 17 | 9 | 25 | – | 12 | 16 | 10 | 18 | 1 | 1 | 0 | 1 | 3 | 0 | 2 | 0 | 1 | 1 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 3 | 4 | 2 | 1 | 2 | 1 | – | 0 | 1 | 1 | 3 | 2 | 0 | 1 | 2 | 4 | 6 | 1 | 2 | 4 | 1 | 1 | 1 |
Cytoplasm | 404 | 353 | 298 | 290 | 353 | 358 | 402 | – | 252 | 240 | 208 | 264 | 274 | 395 | 331 | 135 | 232 | 189 | 353 | 319 | 173 | 216 | 322 | 334 |
Endoplasmic Reticulum | 2 | 1 | 0 | 2 | 0 | 1 | 2 | – | 1 | 0 | 1 | 5 | 0 | 1 | 1 | 8 | 24 | 17 | 0 | 0 | 3 | 0 | 1 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 6 | 4 | 1 | 3 | 1 | 4 | 1 |
Golgi | 1 | 0 | 1 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 1 |
Mitochondria | 12 | 1 | 0 | 2 | 1 | 9 | 23 | – | 34 | 33 | 99 | 66 | 0 | 1 | 0 | 10 | 1 | 2 | 20 | 11 | 65 | 5 | 9 | 10 |
Nucleus | 0 | 1 | 2 | 0 | 1 | 4 | 3 | – | 1 | 2 | 1 | 1 | 1 | 0 | 2 | 4 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 2 | 3 | 3 | 3 | 7 | – | 7 | 7 | 11 | 19 | 0 | 0 | 2 | 0 | 0 | 1 | 5 | 2 | 5 | 2 | 7 | 7 |
Vac/Vac Membrane | 0 | 1 | 3 | 2 | 1 | 5 | 0 | – | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 4 | 2 | 4 | 6 | 2 | 5 | 0 | 3 | 2 |
Unique Cell Count | 416 | 357 | 303 | 297 | 359 | 376 | 427 | 283 | 277 | 284 | 322 | 274 | 398 | 334 | 147 | 240 | 200 | 413 | 354 | 288 | 246 | 375 | 390 | |
Labelled Cell Count | 423 | 361 | 323 | 313 | 378 | 393 | 468 | 311 | 303 | 347 | 380 | 278 | 399 | 337 | 167 | 271 | 227 | 413 | 354 | 288 | 246 | 375 | 390 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.3 | 4.0 | 4.8 | 4.5 | 4.7 | 4.1 | 4.0 | – | 3.6 | 3.5 | 3.1 | 3.3 | 4.8 | 5.0 | 5.2 | 5.2 | 5.3 | 5.3 | 4.6 | 4.7 | 4.8 |
Std Deviation (1e-4) | 0.4 | 0.7 | 1.3 | 1.6 | 1.7 | 1.9 | 1.5 | – | 1.6 | 1.2 | 1.2 | 0.9 | 1.0 | 1.2 | 5.0 | 1.5 | 1.6 | 1.9 | 1.4 | 1.3 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.01 | -0.22 | -0.24 | – | -0.41 | -0.43 | -0.61 | -0.54 | 0.01 | 0.07 | 0.12 | 0.13 | 0.14 | 0.16 | -0.06 | -0.02 | 0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.4479 | -1.4114 | -1.5123 | -1.5851 | -1.3124 | -1.364 | -0.6805 | -0.5376 | -0.4421 | -0.7645 | -1.0737 | -0.5048 | -1.5284 | -1.1673 | -1.2435 | -0.8502 | -1.4146 | -1.1255 |
Actin | 0.0183 | 0.0013 | 0.004 | 0.0205 | 0.0141 | 0.0098 | 0.0351 | 0.0017 | 0.0135 | 0.0019 | 0.0002 | 0.0018 | 0.0019 | 0.0004 | 0.0005 | 0.013 | 0.0005 | 0.001 |
Bud | 0.0007 | 0.0013 | 0.0016 | 0.0002 | 0.0019 | 0.0009 | 0.0009 | 0.0017 | 0.0017 | 0.001 | 0.0046 | 0.0023 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0005 | 0.0004 |
Bud Neck | 0.0028 | 0.0003 | 0.0005 | 0.0004 | 0.0002 | 0.0012 | 0.0026 | 0.0003 | 0.0005 | 0.0003 | 0.0034 | 0.0011 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0.0003 | 0.0008 |
Bud Periphery | 0.0022 | 0.0044 | 0.0034 | 0.0003 | 0.0056 | 0.0007 | 0.0018 | 0.0018 | 0.0058 | 0.0026 | 0.0063 | 0.0023 | 0.0002 | 0.0003 | 0.0008 | 0.0002 | 0.0009 | 0.0006 |
Bud Site | 0.005 | 0.0137 | 0.016 | 0.0038 | 0.003 | 0.0002 | 0.0095 | 0.0227 | 0.0051 | 0.0045 | 0.0073 | 0.0021 | 0.0018 | 0.0041 | 0.0021 | 0.0004 | 0.0005 | 0.0012 |
Cell Periphery | 0.0006 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.018 | 0.0224 | 0.015 | 0.0158 | 0.0076 | 0.0293 | 0.0075 | 0.0093 | 0.0078 | 0.0085 | 0.0169 | 0.0115 | 0.0229 | 0.0246 | 0.0239 | 0.0342 | 0.0671 | 0.0413 |
Cytoplasmic Foci | 0.0221 | 0.0071 | 0.0126 | 0.0211 | 0.0394 | 0.0126 | 0.0172 | 0.0027 | 0.0065 | 0.005 | 0.0069 | 0.0103 | 0.0077 | 0.0096 | 0.0034 | 0.009 | 0.0103 | 0.0034 |
Eisosomes | 0.0007 | 0.0005 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0006 | 0.0004 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0073 | 0.0011 | 0.0004 | 0.0002 | 0.0001 | 0.0006 | 0.0013 | 0.0001 | 0.0005 | 0.0006 | 0.0003 | 0.0005 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0003 |
Endosome | 0.0255 | 0.0009 | 0.0078 | 0.0033 | 0.0007 | 0.0078 | 0.0115 | 0 | 0.0021 | 0.0015 | 0.0005 | 0.0083 | 0.0016 | 0.0004 | 0.0004 | 0.0028 | 0.0004 | 0.0004 |
Golgi | 0.0105 | 0.0002 | 0.0041 | 0.0044 | 0.0014 | 0.0017 | 0.0036 | 0.0001 | 0.0021 | 0.0004 | 0.0001 | 0.0015 | 0.0004 | 0 | 0.0001 | 0.0032 | 0.0001 | 0.0001 |
Lipid Particles | 0.0175 | 0.0008 | 0.0081 | 0.0042 | 0.0013 | 0.0027 | 0.0118 | 0.0002 | 0.0039 | 0.0007 | 0.0008 | 0.0031 | 0.0024 | 0.0001 | 0.0002 | 0.0079 | 0.0005 | 0.0001 |
Mitochondria | 0.0091 | 0.0006 | 0.0219 | 0.002 | 0.0048 | 0.0007 | 0.0028 | 0.0011 | 0.0078 | 0.0019 | 0.0004 | 0.0046 | 0.0003 | 0.0002 | 0.0006 | 0.0022 | 0.0007 | 0.0003 |
None | 0.8351 | 0.9361 | 0.8956 | 0.8891 | 0.8787 | 0.9141 | 0.8688 | 0.9527 | 0.9188 | 0.9608 | 0.9187 | 0.9305 | 0.9542 | 0.9563 | 0.9627 | 0.9087 | 0.9 | 0.946 |
Nuclear Periphery | 0.003 | 0.0004 | 0.0003 | 0.0004 | 0.0001 | 0.0025 | 0.0029 | 0.0001 | 0.0009 | 0.0007 | 0.0004 | 0.0054 | 0.0003 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0004 |
Nucleolus | 0.0007 | 0.0009 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0014 | 0.0003 | 0.001 | 0.001 | 0.0063 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0023 | 0.0001 |
Nucleus | 0.0011 | 0.003 | 0.0005 | 0.0004 | 0.0001 | 0.0016 | 0.001 | 0.0004 | 0.0037 | 0.0013 | 0.0057 | 0.0024 | 0.0004 | 0.0004 | 0.0009 | 0.0003 | 0.0014 | 0.0006 |
Peroxisomes | 0.0135 | 0.0007 | 0.0055 | 0.0272 | 0.039 | 0.0046 | 0.0088 | 0.0033 | 0.0116 | 0.0005 | 0.0003 | 0.0024 | 0.0025 | 0.0001 | 0.0005 | 0.0154 | 0.0013 | 0.0002 |
Punctate Nuclear | 0.0017 | 0.0034 | 0.0011 | 0.0059 | 0.0013 | 0.0069 | 0.0079 | 0.0009 | 0.0046 | 0.0047 | 0.0198 | 0.0084 | 0.002 | 0.001 | 0.0021 | 0.0013 | 0.0124 | 0.0023 |
Vacuole | 0.0034 | 0.0005 | 0.0004 | 0.0001 | 0.0001 | 0.001 | 0.002 | 0.0001 | 0.0008 | 0.0012 | 0.0006 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0002 |
Vacuole Periphery | 0.0013 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0005 | 0.0007 | 0 | 0.0003 | 0.0004 | 0.0001 | 0.0005 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 38.3536 | 22.8572 | 29.3556 | 38.2123 | 29.645 | 28.0121 | 29.7775 | 36.146 | 37.6576 | 36.3373 |
Translational Efficiency | 0.4167 | 0.6247 | 0.5913 | 0.4923 | 0.4012 | 0.5763 | 0.6337 | 0.4534 | 0.4111 | 0.399 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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