Standard name
Human Ortholog
Description 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP; which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication; a missense mutation in the conserved residue R196 of its human homolog PRPS1 is pathogenic

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.05 0.05 0 0 0 0 0 0 0.06 0 0 0 0.07 0.08 0.09 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.95 0.95 0.88 0.83 0.69 0.71 0.66 0.62 0.57 0.63 0.96 0.99 0.98 0.89 0.91 0.94 0.94 0.94 0.9 0.83 0.88 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.06 0.23 0.39 0.31 0.45 0.45 0.54 0.39 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0 0.06 0.05 0 0.05 0 0.06 0 0 0 0.1 0.07 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 1 2 1 4 6 2 3 5 9 0 0 1 0 1 1 0 1 0 0 0 1
Bud 0 0 1 0 1 2 9 4 5 17 12 10 0 0 0 0 1 0 0 0 1 1 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3 13 5 10 17 5 16 15 11 14 11 20 2 5 4 27 24 27 0 0 0 0 0 0
Cytoplasm 174 418 214 202 300 259 253 273 230 259 212 223 186 198 186 326 284 291 170 403 212 150 245 323
Endoplasmic Reticulum 1 8 2 3 2 7 7 9 8 0 8 5 3 0 1 13 10 6 4 6 10 13 13 15
Endosome 0 0 1 0 0 2 2 3 0 4 0 2 0 0 0 1 2 0 0 0 2 0 0 0
Golgi 0 1 0 0 2 1 0 0 1 0 0 0 1 0 0 3 1 3 0 1 0 0 1 0
Mitochondria 2 1 0 1 19 71 143 119 158 188 199 140 1 0 0 3 2 10 0 1 2 3 6 9
Nucleus 1 1 0 0 1 1 2 3 2 2 4 1 3 2 2 1 1 0 0 0 1 0 0 0
Nuclear Periphery 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0
Nucleolus 0 0 0 0 0 0 1 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 1 9 3 23 11 21 21 10 21 8 23 0 1 1 38 21 10 1 8 2 6 5 7
Unique Cell Count 178 430 225 212 339 313 366 384 348 420 370 356 193 200 189 368 312 311 181 429 237 181 279 364
Labelled Cell Count 183 444 232 220 367 361 458 454 427 510 459 434 196 206 195 413 347 348 181 429 237 181 279 364


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.7 13.9 10.3 9.9 9.1 7.2 6.2 6.9 6.0 5.9 5.2 6.0 11.1 11.8 11.3 16.4 16.4 16.6 12.1 11.9 12.9
Std Deviation (1e-4) 1.6 2.0 1.3 1.4 1.8 1.4 1.1 1.4 1.5 1.0 1.0 1.0 1.9 1.7 1.8 2.9 3.5 2.9 2.8 2.7 2.7
Intensity Change (Log2) -0.06 -0.17 -0.53 -0.73 -0.59 -0.78 -0.8 -0.98 -0.78 0.11 0.2 0.13 0.67 0.67 0.69 0.23 0.21 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 2.6 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.4 1.7 0 1.4 1.1 0.7 0.8 0.5 2.0 0 0 0 2.7 2.8 3.1
Cytoplasm 0.1 -2.7 -4.3 -7.5 -7.1 -8.1 -9.2 -9.9 -8.8 0.6 2.3 1.9 -2.7 -1.8 -0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.0 1.8 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.6 7.7 10.8 9.3 11.9 11.9 13.5 10.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.7 1.4 -0.3 0.9 0.8 -0.7 0.6 -1.3 1.3 0 0 0 2.8 1.4 -0.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.7823 11.7631 10.167 9.5273 8.8813 10.3138 7.7521 10.489 9.7113 8.9405 8.7738 9.297 7.9272 11.8307 10.3195 9.5262 9.5554 9.9038
Actin 0.012 0.0009 0.011 0.0228 0.0127 0.0012 0.0062 0.0015 0.0002 0.0002 0.0034 0.0013 0.0332 0.0003 0.0254 0.0074 0.0004 0.0029
Bud 0.0005 0.0003 0.0007 0.002 0.0003 0.0004 0.0009 0.0024 0.0004 0.0006 0.0004 0.0016 0.0011 0.0002 0.0007 0.0006 0.0004 0.0003
Bud Neck 0.0006 0.0003 0.0007 0.0008 0.0013 0.0011 0.0013 0.0003 0.0004 0.0004 0.0008 0.0025 0.0019 0.0002 0.002 0.0009 0.0007 0.0012
Bud Periphery 0.0005 0.0001 0.0005 0.002 0.0002 0.0003 0.0006 0.0007 0.0001 0.0001 0.0005 0.0011 0.0016 0 0.0006 0.0003 0.0001 0.0003
Bud Site 0.0009 0.001 0.0021 0.0029 0.0003 0.0003 0.0009 0.0102 0.0004 0.0007 0.0004 0.0022 0.0051 0.0006 0.0027 0.0012 0.0002 0.0002
Cell Periphery 0.0005 0.0001 0.0003 0.0002 0.0001 0.0001 0.0005 0.0002 0.0001 0.0001 0.0001 0.0003 0.0011 0.0002 0.0005 0.0002 0 0.0001
Cytoplasm 0.8375 0.967 0.8864 0.779 0.8301 0.8996 0.8784 0.9652 0.9439 0.858 0.8804 0.9088 0.7167 0.9786 0.8922 0.8781 0.8584 0.8795
Cytoplasmic Foci 0.0244 0.0069 0.0139 0.0199 0.0181 0.0145 0.0097 0.0038 0.012 0.0133 0.0065 0.0095 0.0311 0.0038 0.0155 0.0153 0.0161 0.0141
Eisosomes 0.0001 0 0.0003 0.0001 0.0001 0 0.0001 0 0 0 0.0001 0 0.0004 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0225 0.0044 0.008 0.0151 0.015 0.016 0.021 0.0032 0.0057 0.0037 0.012 0.029 0.0397 0.0046 0.0084 0.0085 0.0151 0.0217
Endosome 0.0457 0.0105 0.0362 0.0775 0.047 0.0331 0.0294 0.0048 0.0202 0.0453 0.0287 0.0226 0.0594 0.0046 0.0246 0.0253 0.0718 0.0396
Golgi 0.0069 0.0016 0.0044 0.0047 0.0032 0.0064 0.0028 0.0004 0.0009 0.0008 0.0018 0.0023 0.0076 0.0008 0.0047 0.0032 0.004 0.0138
Lipid Particles 0.0028 0 0.0018 0.0052 0.0029 0.002 0.0017 0 0.0001 0.0001 0.0009 0.0001 0.0081 0.0001 0.0019 0.0043 0.0004 0.002
Mitochondria 0.0077 0.0004 0.0103 0.0053 0.0438 0.0101 0.0022 0.0002 0.0004 0.0002 0.005 0.0029 0.0145 0.0002 0.0043 0.0008 0.0019 0.0109
None 0.0088 0.0007 0.003 0.003 0.0016 0.0009 0.0065 0.0008 0.0011 0.0011 0.0014 0.0008 0.0026 0.0005 0.0008 0.0016 0.0007 0.0005
Nuclear Periphery 0.0082 0.0006 0.0033 0.01 0.0026 0.0011 0.0081 0.0006 0.0011 0.0023 0.0315 0.0055 0.0154 0.0006 0.0015 0.0048 0.0053 0.0036
Nucleolus 0.0005 0 0.0005 0.0005 0 0 0.0007 0 0 0.0001 0.0006 0.0001 0.0066 0 0.0001 0.0003 0 0
Nucleus 0.0051 0.001 0.0025 0.0092 0.0018 0.0014 0.009 0.001 0.0012 0.0034 0.0095 0.0029 0.0199 0.0009 0.0011 0.0026 0.0031 0.0013
Peroxisomes 0.0016 0.0001 0.0017 0.0049 0.0046 0.0016 0.0005 0.0001 0.0001 0 0.0001 0.0001 0.0076 0 0.0021 0.0025 0.0002 0.0012
Punctate Nuclear 0.003 0.0001 0.0031 0.0019 0.0005 0.0002 0.0042 0.0001 0.0001 0.0003 0.0014 0.0002 0.0033 0.0001 0.0013 0.0102 0.0005 0.0002
Vacuole 0.0085 0.0032 0.0078 0.0276 0.0116 0.0078 0.0131 0.004 0.0103 0.0638 0.0088 0.005 0.0185 0.0036 0.008 0.0291 0.0164 0.0044
Vacuole Periphery 0.0017 0.0005 0.0016 0.0053 0.0023 0.0019 0.0023 0.0005 0.0013 0.0057 0.0058 0.0013 0.0046 0.0003 0.0014 0.0027 0.0042 0.0022

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 51.1773 43.2357 51.4816 42.7446 43.0204 42.7178 33.8033 31.9066 34.7427 45.0882
Translational Efficiency 1.6573 1.699 1.4016 1.4218 1.4144 1.528 2.0102 1.6952 1.5171 1.4382

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP; which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication; a missense mutation in the conserved residue R196 of its human homolog PRPS1 is pathogenic
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Prs4

Prs4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prs4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available