Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.06 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0.05 0.06 0.05 0.07 0.09 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.0 0.98 0.99 0.91 0.83 0.78 0.79 0.61 0.71 0.67 1.0 1.0 0.98 0.99 0.99 1.0 0.97 0.95 0.95 0.92 0.86 0.93
Endoplasmic Reticulum 0 0 0 0 0.06 0.11 0.14 0.14 0.17 0.14 0.2 0 0 0 0.05 0 0.07 0 0 0 0 0.06 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0.07 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 4 4 10 21 18 23 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 0 0 1 4 0 1 2 0 1 0 0 0 0 0 0 0 0 1 0 5 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3 1 4 0 3 14 25 17 25 28 26 0 1 1 1 1 0 0 0 1 0 0 0
Cytoplasm 242 241 262 202 282 254 311 247 214 228 239 115 230 251 184 155 112 228 233 241 123 169 183
Endoplasmic Reticulum 1 0 4 2 20 35 57 43 60 44 71 0 1 4 10 5 8 1 0 4 5 11 6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 1 4 10 10 24 22 19 0 0 1 0 1 0 0 0 0 0 0 1
Mitochondria 0 0 0 0 0 1 0 3 3 8 8 0 0 0 1 0 2 0 0 1 0 0 0
Nucleus 0 0 0 0 0 1 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 2 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 1 0 3 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 3 3 4 1 24 3 6 0 0 1 0 1 0 1 5 0 1 1 2
Unique Cell Count 243 242 266 204 310 306 399 313 349 323 355 115 230 256 185 156 112 236 246 256 135 196 196
Labelled Cell Count 247 242 270 204 311 320 412 332 378 352 395 115 232 258 196 163 122 236 246 256 135 196 196


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 186.2 154.3 146.0 132.2 99.9 60.7 63.2 52.1 43.5 47.1 45.2 164.5 166.0 156.9 213.3 216.0 210.5 163.8 176.6 190.9
Std Deviation (1e-4) 41.9 39.2 27.3 31.9 22.0 18.0 14.0 11.7 15.9 11.8 10.5 38.5 45.8 34.5 62.2 58.2 63.8 45.1 50.2 48.3
Intensity Change (Log2) -0.14 -0.55 -1.27 -1.21 -1.49 -1.75 -1.63 -1.69 0.17 0.18 0.1 0.55 0.57 0.53 0.17 0.27 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 4.1 3.9 4.2 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2.1 2.9 2.5 3.3 3.8 3.3 0 0 0 0 0 0
Cytoplasm 0.5 -3.9 -6.2 -7.5 -7.2 -10.9 -9.0 -9.7 1.3 1.9 -0.4 1.0 0.8 1.3
Endoplasmic Reticulum 0 3.0 4.7 5.6 5.4 6.3 5.3 7.0 0 0 0 2.3 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 4.4 4.3 3.8 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 4.4 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 127.6041 192.0338 157.036 147.8131 126.4419 88.4947 169.7794 215.4273 197.8487 168.7029 155.9453 187.2596 166.3584 219.7094 177.5843 151.9423 202.3388 123.3687
Actin 0.0179 0.0006 0.0132 0.0003 0.0832 0.0027 0.0032 0.0005 0.0012 0.0008 0.0083 0.0014 0.0038 0.0007 0.0138 0.0051 0.0021 0.0036
Bud 0.0041 0.0015 0.0029 0.001 0.0047 0.0123 0.0026 0.0015 0.0031 0.0014 0.0014 0.0044 0.0053 0.0022 0.004 0.0038 0.0057 0.0128
Bud Neck 0.0015 0.0007 0.003 0.0007 0.002 0.0017 0.0009 0.0005 0.0015 0.001 0.0018 0.0032 0.0009 0.0006 0.0009 0.0029 0.0009 0.0009
Bud Periphery 0.0038 0.0016 0.0049 0.0011 0.0057 0.0057 0.0038 0.0013 0.0034 0.0013 0.0032 0.0018 0.0104 0.0011 0.0047 0.0053 0.0028 0.051
Bud Site 0.0016 0.0007 0.0082 0.0003 0.0016 0.0019 0.0017 0.0009 0.0012 0.0014 0.0006 0.0004 0.0014 0.0008 0.0013 0.0025 0.0005 0.0008
Cell Periphery 0.0613 0.0322 0.0288 0.0175 0.0086 0.007 0.0845 0.0392 0.0439 0.0709 0.0138 0.016 0.0377 0.0168 0.0324 0.035 0.0072 0.0301
Cytoplasm 0.4858 0.8227 0.7035 0.5695 0.5687 0.3574 0.5206 0.8466 0.711 0.5274 0.7033 0.7173 0.6359 0.8532 0.6703 0.4465 0.5438 0.621
Cytoplasmic Foci 0.0257 0.0011 0.0087 0.0254 0.0209 0.0694 0.0094 0.0011 0.0079 0.0185 0.0063 0.0051 0.0041 0.0014 0.003 0.0225 0.0055 0.008
Eisosomes 0.0005 0.0002 0.0004 0.0004 0.0007 0.0007 0.001 0 0.0002 0.0002 0.0004 0 0.0006 0 0.0002 0.0038 0 0.0007
Endoplasmic Reticulum 0.047 0.0162 0.0131 0.0038 0.0297 0.0087 0.0423 0.0092 0.017 0.005 0.0069 0.0646 0.0246 0.0038 0.0101 0.0227 0.0098 0.0159
Endosome 0.0131 0.0026 0.0136 0.006 0.0104 0.1607 0.0096 0.0025 0.0039 0.0066 0.0147 0.0143 0.0093 0.0029 0.0124 0.0095 0.0266 0.0194
Golgi 0.0029 0.0012 0.0043 0.0004 0.0115 0.0128 0.0034 0.0004 0.0012 0.0023 0.0034 0.0028 0.0023 0.0004 0.0078 0.0016 0.004 0.0047
Lipid Particles 0.0067 0.0019 0.003 0.0079 0.0264 0.0138 0.0067 0.0023 0.0037 0.0202 0.0193 0.0039 0.0067 0.0007 0.0024 0.0187 0.0012 0.0039
Mitochondria 0.0112 0.004 0.0167 0.0452 0.0588 0.0393 0.0134 0.0016 0.0068 0.0055 0.0124 0.0057 0.017 0.0029 0.0174 0.031 0.008 0.0355
None 0.0186 0.0007 0.0056 0.0021 0.0227 0.0117 0.0035 0.0005 0.0039 0.0044 0.0143 0.0009 0.0143 0.0007 0.0015 0.0197 0.001 0.0043
Nuclear Periphery 0.0442 0.0075 0.0074 0.0129 0.0132 0.0553 0.0286 0.0041 0.0104 0.0201 0.019 0.0234 0.0258 0.0036 0.0051 0.0136 0.0318 0.0126
Nucleolus 0.0135 0.0009 0.0086 0.0163 0.0145 0.0341 0.0079 0.0004 0.0021 0.0196 0.0146 0.003 0.0127 0.0005 0.003 0.036 0.0024 0.0012
Nucleus 0.0208 0.0044 0.0096 0.0084 0.0052 0.0202 0.01 0.0023 0.0054 0.0182 0.0093 0.0063 0.0138 0.0038 0.0074 0.0196 0.0137 0.005
Peroxisomes 0.0026 0.0001 0.0029 0.0025 0.0451 0.0027 0.0016 0 0.0008 0.0074 0.0008 0.0001 0.0016 0.0001 0.0007 0.0032 0.0005 0.0004
Punctate Nuclear 0.0031 0 0.0008 0.0002 0.0008 0.0051 0.0004 0 0.0004 0.0011 0.0052 0.0003 0.0014 0 0.0001 0.0023 0.0001 0.0001
Vacuole 0.1655 0.089 0.1162 0.1961 0.0454 0.1158 0.2134 0.0785 0.1497 0.2368 0.1089 0.1011 0.1243 0.0866 0.1566 0.2106 0.1869 0.1034
Vacuole Periphery 0.0486 0.0101 0.0244 0.0819 0.0201 0.061 0.0315 0.0064 0.0213 0.03 0.0322 0.0237 0.0461 0.017 0.045 0.084 0.1455 0.0646

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4693.5861 5005.491 5922.2296 4442.4893 5213.5491 4921.4222 4385.8271 3967.8738 4583.7889 3739.709
Translational Efficiency 0.7598 0.7356 0.6315 0.8509 0.7795 0.7335 0.7484 0.8486 0.7508 0.9079

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Rps8a

Rps8a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps8a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available