Standard name
Human Ortholog
Description ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.06 0 0 0.07 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.99 0.98 0.96 0.95 0.93 0.95 0.82 0.83 0.73 0.76 0.98 1.0 0.99 0.91 0.92 0.93 0.86 0.86 0.63 0.88 0.89 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0.1 0.05 0.24 0.21 0.34 0.36 0 0 0 0.07 0 0 0.06 0 0.17 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 1 1 5 3 2 2 5
Bud 1 1 0 0 0 1 2 3 1 1 3 3 0 0 0 0 0 1 1 5 2 4 5 13
Bud Neck 0 2 5 3 8 6 7 14 5 11 0 6 1 0 0 1 2 1 0 2 0 0 2 5
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 3 1 0 1 0 0 1 1 0 1 1 1 1 0 8 3 6 1 3 2 0 3 1
Cytoplasm 202 475 164 110 134 177 180 204 112 164 70 134 153 233 240 301 302 260 183 422 101 191 268 291
Endoplasmic Reticulum 1 1 1 1 2 2 0 0 0 0 0 0 1 0 2 20 18 22 1 0 2 0 0 1
Endosome 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 4 6 6 1 7 2 1 0 4
Golgi 0 1 0 0 0 0 0 1 0 0 0 0 1 0 0 5 2 1 0 3 3 1 0 2
Mitochondria 4 2 0 4 2 19 20 11 33 42 33 63 0 0 0 24 5 6 13 16 26 4 5 12
Nucleus 0 1 1 0 3 2 1 2 1 2 3 2 0 0 0 1 0 3 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 0 0 0 0 0
SpindlePole 2 0 1 1 2 1 2 3 0 0 5 1 0 0 0 3 1 4 0 6 1 1 3 7
Vac/Vac Membrane 1 0 1 0 0 0 1 0 1 5 1 1 0 0 1 12 20 9 1 6 3 2 3 3
Unique Cell Count 206 481 166 112 140 186 193 214 137 197 96 177 156 233 243 332 330 281 213 490 160 216 301 362
Labelled Cell Count 213 489 174 119 152 208 214 239 155 226 116 212 158 234 243 380 363 320 213 490 160 216 301 362


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.4 5.2 4.8 5.6 4.1 4.2 6.2 4.3 3.8 4.2 3.7 5.2 5.2 5.2 7.1 8.9 6.5 4.9 5.2 5.4
Std Deviation (1e-4) 1.1 2.1 1.8 1.9 2.9 2.1 2.0 18.2 7.5 1.9 4.4 2.1 1.3 1.3 1.4 7.9 15.7 5.2 1.2 1.8 3.0
Intensity Change (Log2) – – – -0.09 0.12 -0.32 -0.29 0.27 -0.27 -0.43 -0.28 -0.47 0.02 0.0 0.02 0.46 0.79 0.33 -0.07 0.01 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 –
Bud Neck – – – – – – – – – – – – – 0 –
Bud Site – – – – – – – – – – – – – 0 –
Cell Periphery – – – – – – – – – – – – – 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 –
Endosome – – – – – – – – – – – – – 0 –
Golgi – – – – – – – – – – – – – 0 –
Mitochondria – – – – – – – – – – – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 –
Peroxisomes – – – – – – – – – – – – – 0 –
SpindlePole – – – – – – – – – – – – – 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 –
Cytoplasm – – – – – – – – – – – – – 0 –
Nucleus – – – – – – – – – – – – – 0 –
Vacuole – – – – – – – – – – – – – 2.8 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4389 -0.9365 -1.2237 -1.0157 -0.6188 -1.1357 -0.6119 -0.9477 -0.7948 -0.7335 -1.4159 -0.978 -0.8876 -0.8819 -0.8828 -0.6437 -1.0193 -1.0103
Actin 0.0164 0.0032 0.0067 0.0007 0.0468 0.0034 0.0161 0.0051 0.0099 0.0267 0.0001 0.0075 0.054 0.0074 0.0351 0.0426 0.0242 0.0089
Bud 0.0008 0.0035 0.0005 0.0007 0.002 0.0003 0.0008 0.0013 0.0004 0.0025 0 0.0002 0.0017 0.0027 0.0009 0.0001 0.0003 0.0007
Bud Neck 0.0048 0.0006 0.0007 0.0008 0.0019 0.0019 0.0123 0.0005 0.0005 0.0004 0 0.0026 0.0022 0.0003 0.0024 0.0001 0.0002 0.0002
Bud Periphery 0.0013 0.0048 0.0012 0.0011 0.0034 0.0014 0.0014 0.0022 0.0007 0.0035 0.0001 0.0003 0.0022 0.0042 0.0017 0.0002 0.0004 0.0008
Bud Site 0.008 0.0146 0.0008 0.016 0.0094 0.0002 0.0102 0.0075 0.0037 0.0021 0 0.0004 0.0099 0.0072 0.0042 0.0001 0.0014 0.0001
Cell Periphery 0.0005 0.0004 0.0003 0.0001 0.0003 0.0001 0.0009 0.0002 0.0002 0.0001 0 0.0001 0.0004 0.0004 0.0004 0 0.0001 0.0001
Cytoplasm 0.0472 0.0983 0.025 0.0762 0.0128 0.0786 0.0163 0.0298 0.0247 0.0092 0.0048 0.0496 0.07 0.0877 0.1241 0.1885 0.0768 0.1448
Cytoplasmic Foci 0.0543 0.0118 0.0125 0.0389 0.0514 0.0053 0.0383 0.0128 0.009 0.0142 0.0014 0.0101 0.0205 0.002 0.0043 0.0084 0.0071 0.0078
Eisosomes 0.0005 0.0006 0.0005 0.0001 0.0011 0.0002 0.0004 0.0002 0.0003 0.0003 0.0001 0.0001 0.0007 0.0002 0.0006 0.0002 0.0003 0.0002
Endoplasmic Reticulum 0.0039 0.0009 0.0006 0.001 0.0023 0.0005 0.0026 0.0004 0.002 0.0005 0.0001 0.0004 0.0033 0.0005 0.0018 0.0006 0.0007 0.0012
Endosome 0.0398 0.0044 0.0031 0.0085 0.0579 0.0024 0.03 0.0049 0.0136 0.0404 0.0001 0.0044 0.0058 0.0001 0.0039 0.0006 0.0029 0.0059
Golgi 0.0185 0.0052 0.0025 0.0028 0.0206 0.0005 0.0089 0.0101 0.0029 0.0146 0 0.003 0.0034 0.0001 0.0027 0.0002 0.0049 0.0025
Lipid Particles 0.0467 0.0013 0.0039 0.02 0.0156 0.0031 0.0197 0.0014 0.0037 0.0007 0.0003 0.0028 0.004 0.0001 0.0052 0.0011 0.0087 0.0025
Mitochondria 0.0256 0.0024 0.0153 0.0324 0.0888 0.0022 0.0164 0.0032 0.0072 0.0068 0.0001 0.0007 0.0013 0.0004 0.0019 0.0005 0.0044 0.0012
None 0.6492 0.8415 0.9078 0.7607 0.5971 0.8856 0.774 0.9139 0.9073 0.843 0.9924 0.9082 0.7953 0.8834 0.801 0.7518 0.8567 0.8112
Nuclear Periphery 0.0094 0.0004 0.0005 0.0009 0.0057 0.0019 0.0081 0.0002 0.0023 0.0008 0.0001 0.0002 0.0058 0.0003 0.0019 0.0004 0.0007 0.0026
Nucleolus 0.0035 0.0006 0.0004 0.0014 0.0064 0.0007 0.0031 0.0003 0.0004 0.0002 0.0001 0.0001 0.0007 0.0002 0.0005 0.0002 0.0002 0.0001
Nucleus 0.0088 0.0016 0.0044 0.0027 0.0085 0.0022 0.0038 0.0004 0.002 0.0005 0.0001 0.0014 0.0065 0.0014 0.0012 0.0009 0.0006 0.0006
Peroxisomes 0.0405 0.0007 0.0049 0.0055 0.0333 0.0055 0.0145 0.0039 0.0064 0.0067 0.0001 0.0068 0.0048 0.0003 0.0033 0.0017 0.0068 0.0069
Punctate Nuclear 0.0107 0.0021 0.0049 0.0276 0.0285 0.0034 0.0093 0.0007 0.0005 0.0007 0.0001 0.0007 0.0064 0.0007 0.002 0.0012 0.002 0.0009
Vacuole 0.0072 0.0009 0.0025 0.0016 0.0042 0.0004 0.0114 0.0009 0.0016 0.0172 0.0001 0.0003 0.0008 0.0003 0.0006 0.0004 0.0003 0.0004
Vacuole Periphery 0.0024 0.0001 0.0008 0.0004 0.0017 0.0002 0.0017 0.0002 0.0006 0.0088 0 0.0001 0.0003 0 0.0002 0.0001 0.0002 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.2048 9.6108 12.0112 10.3452 13.5725 11.8762 10.9961 10.701 10.9915 9.8072
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Ssa3

Ssa3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ssa3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available