Standard name
Human Ortholog
Description Subunit of inner ring of nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; interacts with genomic regions that contain ribosomal protein and subtelomeric genes, where it functions in nucleosome positioning and as a repressor of transcription; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.1 0.14 0.07 0.12 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0.06 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0.14 0.17 0.22 0.58 0.58 0.56 0.68 0.65 0.62 0.62 0 0 0 0.08 0.12 0.07 0 0 0 0.12 0.18 0.13
Nucleus 0 0 0 0 0 0 0 0.05 0 0.06 0.05 0 0.05 0.05 0 0.05 0.09 0 0 0 0 0 0 0
Nuclear Periphery 0.78 0.81 0.8 0.81 0.71 0.54 0.53 0.45 0.49 0.45 0.46 0.55 0.76 0.76 0.69 0.6 0.53 0.41 0.85 0.84 0.86 0.73 0.62 0.62
Nucleolus 0.06 0.07 0.12 0.25 0.18 0.26 0.26 0.26 0.27 0.29 0.29 0.3 0.08 0.08 0.09 0.11 0.14 0.21 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.43 0.33 0.24 0.14 0.19 0.09 0.07 0.06 0.07 0 0.05 0 0.21 0.23 0.27 0.31 0.29 0.38 0 0.06 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 0 1 0 0 1 1 0 0 0 1 2 1 2 3
Bud 0 1 0 0 0 1 4 5 3 1 2 3 0 0 0 1 2 0 2 3 1 1 4 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 1 0 0 0 0 0
Bud Site 0 0 1 0 1 5 7 5 14 7 10 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 6 8 7 10 8 16 10 1 6 5 7 15 15 21 18 30 16 0 0 0 0 1 1
Endoplasmic Reticulum 0 4 0 0 0 3 2 1 2 1 1 0 0 1 1 7 6 1 0 0 2 1 3 3
Endosome 2 0 15 2 20 6 9 11 5 0 5 6 4 4 2 16 13 2 7 7 11 11 26 41
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 6 2 1 11 10 10
Mitochondria 17 27 49 40 81 263 373 380 306 355 255 301 1 1 1 21 29 6 12 17 14 39 72 66
Nucleus 11 19 7 8 10 14 24 34 20 33 19 14 7 7 3 13 21 4 3 8 1 5 0 5
Nuclear Periphery 296 380 271 188 261 247 341 309 218 245 190 264 107 110 104 154 130 38 368 435 328 248 255 322
Nucleolus 24 31 41 58 68 119 167 178 123 158 121 144 11 11 13 28 35 19 7 8 6 2 4 7
Peroxisomes 0 0 0 0 0 0 1 1 5 3 0 1 0 0 0 1 0 0 0 0 0 3 6 12
SpindlePole 0 0 2 0 3 4 7 6 4 8 5 5 3 3 6 5 2 1 1 1 2 2 1 9
Vac/Vac Membrane 162 154 81 32 71 39 48 41 31 23 22 19 30 33 41 79 71 35 17 29 4 8 20 20
Unique Cell Count 380 469 338 232 369 455 643 680 449 544 411 484 141 144 150 258 246 92 433 521 382 339 413 516
Labelled Cell Count 518 623 475 335 525 709 999 981 733 840 635 772 178 185 195 344 340 122 433 521 382 339 413 516


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.7 12.2 9.1 8.8 8.1 6.7 6.3 6.4 6.3 6.1 6.7 6.3 10.0 10.0 9.9 8.4 8.8 10.8 11.0 11.4 11.7
Std Deviation (1e-4) 2.2 3.3 2.3 2.2 2.1 2.0 1.9 1.6 1.8 1.8 2.2 1.7 2.7 2.7 2.8 1.8 2.1 2.5 2.3 2.2 2.2
Intensity Change (Log2) – – – -0.05 -0.17 -0.45 -0.54 -0.51 -0.54 -0.58 -0.45 -0.53 0.13 0.13 0.11 -0.11 -0.05 0.24 0.27 0.32 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 2.9 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 0.3 -0.6 0.1 -1.0 0 -1.5 -1.2 -1.0 3.9 3.8 5.0 2.7 4.8 5.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -2.5 0.6 -2.7 -2.9 -2.7 -2.9 -5.0 -2.7 -2.9 -0.8 -0.9 -1.7 1.0 0.5 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.9 2.6 12.3 13.1 12.6 15.0 14.7 13.2 13.6 -4.5 -4.5 -4.6 -2.4 -1.0 -2.0
Nucleus 1.0 0.6 0.9 1.4 2.2 1.8 2.8 1.9 0.7 0 0 0 2.0 3.6 0
Nuclear Periphery 0.3 -2.9 -7.6 -8.3 -10.5 -9.1 -10.3 -9.5 -7.6 -1.0 -0.9 -2.6 -5.5 -7.0 -7.4
Nucleolus 4.0 2.3 4.9 5.0 5.1 5.2 5.8 5.7 6.0 -1.4 -1.5 -1.1 -0.5 0.7 2.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.0 -1.5 -6.0 -7.3 -8.3 -6.8 -8.8 -7.4 -8.6 -0.6 -0.2 0.8 1.8 1.3 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.6555 8.1644 7.3642 6.4257 7.4493 7.3111 9.9259 10.5533 9.6711 9.5766 9.3829 9.6202 7.869 10.1328 9.7656 9.6531 9.0591 8.8694
Actin 0.0126 0.0014 0.0063 0.0016 0.0008 0.0001 0.0142 0.0004 0.0097 0.0001 0.002 0 0.138 0.0049 0.0004 0.0012 0.0004 0.017
Bud 0.0003 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0 0 0 0.0015 0.0002 0 0 0 0
Bud Neck 0.0027 0 0.0026 0.0002 0.0001 0 0.0001 0 0.0002 0 0 0 0.0013 0.0001 0 0 0 0
Bud Periphery 0.0004 0 0.0002 0.0003 0.0005 0 0.0001 0.0001 0.0002 0 0.0002 0 0.003 0.0004 0.0001 0.0003 0.0001 0
Bud Site 0.0024 0.0001 0.001 0.0006 0.0004 0 0.0004 0 0.0007 0 0.0001 0 0.0032 0.0006 0 0 0 0
Cell Periphery 0.0002 0 0.0002 0.0002 0.0001 0 0 0 0.0001 0 0.0001 0 0.0005 0.0001 0 0.0001 0 0
Cytoplasm 0.0056 0.0002 0.0002 0.0002 0.0001 0 0.0045 0 0.0003 0 0.0008 0 0.0025 0.0029 0.0001 0.0001 0 0.0004
Cytoplasmic Foci 0.0135 0.0011 0.0039 0.003 0.0012 0 0.0067 0 0.0049 0 0.0049 0 0.0132 0.0227 0 0 0 0.0025
Eisosomes 0.0002 0 0.0004 0.0001 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0.0015 0.0001 0 0.0001 0 0
Endoplasmic Reticulum 0.0051 0.0003 0.0008 0.0014 0.0012 0.0002 0.0009 0.0011 0.001 0.0006 0.0029 0.0001 0.0142 0.0046 0.001 0.0042 0.0002 0.0003
Endosome 0.0199 0.001 0.0058 0.0186 0.0056 0.0002 0.0032 0.0003 0.0025 0.0002 0.0081 0.0001 0.1 0.0186 0.0004 0.0007 0.0003 0.0013
Golgi 0.0053 0.0048 0.0048 0.0042 0.0042 0 0.0014 0.0002 0.0027 0.0001 0.0046 0 0.0442 0.0044 0.0001 0.0002 0.0002 0.0008
Lipid Particles 0.022 0.0107 0.009 0.0348 0.0035 0.0005 0.0048 0.0014 0.0199 0.0032 0.0607 0.0004 0.066 0.0149 0.0009 0.0006 0.0016 0.0015
Mitochondria 0.0063 0.0028 0.0067 0.0245 0.0752 0.0004 0.008 0.0073 0.0029 0.0008 0.0496 0.0002 0.0344 0.0035 0.0018 0.0028 0.0025 0.0004
None 0.002 0.0001 0.0002 0.0003 0.0002 0 0.0078 0.0002 0.0004 0.0001 0.0006 0 0.0036 0.0032 0.0003 0.0004 0.0001 0.0018
Nuclear Periphery 0.8067 0.8673 0.8904 0.8419 0.872 0.9604 0.8869 0.9385 0.8967 0.9529 0.8092 0.983 0.5206 0.7856 0.9317 0.9205 0.9587 0.9465
Nucleolus 0.0028 0.0013 0.0014 0.0026 0.0007 0.001 0.0007 0.0002 0.0005 0.0003 0.0013 0.0002 0.0015 0.001 0.0003 0.0003 0.0026 0.0003
Nucleus 0.0391 0.0701 0.026 0.0198 0.0028 0.0222 0.0114 0.0036 0.0065 0.0056 0.0126 0.0044 0.0111 0.019 0.0148 0.0055 0.0066 0.0018
Peroxisomes 0.0074 0.005 0.0033 0.0013 0.0018 0 0.0103 0 0.0085 0 0.0019 0 0.0048 0.007 0 0 0 0.0027
Punctate Nuclear 0.0212 0.0006 0.0037 0.0013 0.0007 0.0005 0.01 0.0002 0.0019 0.0004 0.004 0.0004 0.0161 0.0535 0.0003 0.0004 0.0011 0.0051
Vacuole 0.0023 0.0008 0.0006 0.0092 0.0012 0.0002 0.0004 0.0005 0.0007 0.0002 0.0013 0 0.0048 0.001 0.0006 0.0009 0.0003 0.0001
Vacuole Periphery 0.0222 0.0323 0.0324 0.034 0.0275 0.0143 0.0279 0.0459 0.0394 0.0353 0.0349 0.0112 0.014 0.0515 0.047 0.0616 0.0251 0.0176

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.5421 33.6886 34.5757 43.4757 40.6117 31.3057 46.7254 41.7998 36.5861 48.996
Translational Efficiency 0.4399 0.5532 0.5802 0.3799 0.3932 0.4635 0.4438 0.3571 0.3776 0.3602

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of inner ring of nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; interacts with genomic regions that contain ribosomal protein and subtelomeric genes, where it functions in nucleosome positioning and as a repressor of transcription; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
Localization
Cell Percentages nuclear periphery (88%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Nup170

Nup170


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nup170-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available