Standard name | |
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Human Ortholog | |
Description | Subunit of inner ring of nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; interacts with genomic regions that contain ribosomal protein and subtelomeric genes, where it functions in nucleosome positioning and as a repressor of transcription; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 3 |
Bud | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 5 | 3 | 1 | 2 | 3 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 3 | 1 | 1 | 4 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 1 | 0 | 1 | 5 | 7 | 5 | 14 | 7 | 10 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 6 | 8 | 7 | 10 | 8 | 16 | 10 | 1 | 6 | 5 | 7 | 15 | 15 | 21 | 18 | 30 | 16 | 0 | 0 | 0 | 0 | 1 | 1 |
Endoplasmic Reticulum | 0 | 4 | 0 | 0 | 0 | 3 | 2 | 1 | 2 | 1 | 1 | 0 | 0 | 1 | 1 | 7 | 6 | 1 | 0 | 0 | 2 | 1 | 3 | 3 |
Endosome | 2 | 0 | 15 | 2 | 20 | 6 | 9 | 11 | 5 | 0 | 5 | 6 | 4 | 4 | 2 | 16 | 13 | 2 | 7 | 7 | 11 | 11 | 26 | 41 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 6 | 2 | 1 | 11 | 10 | 10 |
Mitochondria | 17 | 27 | 49 | 40 | 81 | 263 | 373 | 380 | 306 | 355 | 255 | 301 | 1 | 1 | 1 | 21 | 29 | 6 | 12 | 17 | 14 | 39 | 72 | 66 |
Nucleus | 11 | 19 | 7 | 8 | 10 | 14 | 24 | 34 | 20 | 33 | 19 | 14 | 7 | 7 | 3 | 13 | 21 | 4 | 3 | 8 | 1 | 5 | 0 | 5 |
Nuclear Periphery | 296 | 380 | 271 | 188 | 261 | 247 | 341 | 309 | 218 | 245 | 190 | 264 | 107 | 110 | 104 | 154 | 130 | 38 | 368 | 435 | 328 | 248 | 255 | 322 |
Nucleolus | 24 | 31 | 41 | 58 | 68 | 119 | 167 | 178 | 123 | 158 | 121 | 144 | 11 | 11 | 13 | 28 | 35 | 19 | 7 | 8 | 6 | 2 | 4 | 7 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 3 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 6 | 12 |
SpindlePole | 0 | 0 | 2 | 0 | 3 | 4 | 7 | 6 | 4 | 8 | 5 | 5 | 3 | 3 | 6 | 5 | 2 | 1 | 1 | 1 | 2 | 2 | 1 | 9 |
Vac/Vac Membrane | 162 | 154 | 81 | 32 | 71 | 39 | 48 | 41 | 31 | 23 | 22 | 19 | 30 | 33 | 41 | 79 | 71 | 35 | 17 | 29 | 4 | 8 | 20 | 20 |
Unique Cell Count | 380 | 469 | 338 | 232 | 369 | 455 | 643 | 680 | 449 | 544 | 411 | 484 | 141 | 144 | 150 | 258 | 246 | 92 | 433 | 521 | 382 | 339 | 413 | 516 |
Labelled Cell Count | 518 | 623 | 475 | 335 | 525 | 709 | 999 | 981 | 733 | 840 | 635 | 772 | 178 | 185 | 195 | 344 | 340 | 122 | 433 | 521 | 382 | 339 | 413 | 516 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.7 | 12.2 | 9.1 | 8.8 | 8.1 | 6.7 | 6.3 | 6.4 | 6.3 | 6.1 | 6.7 | 6.3 | 10.0 | 10.0 | 9.9 | 8.4 | 8.8 | 10.8 | 11.0 | 11.4 | 11.7 |
Std Deviation (1e-4) | 2.2 | 3.3 | 2.3 | 2.2 | 2.1 | 2.0 | 1.9 | 1.6 | 1.8 | 1.8 | 2.2 | 1.7 | 2.7 | 2.7 | 2.8 | 1.8 | 2.1 | 2.5 | 2.3 | 2.2 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.17 | -0.45 | -0.54 | -0.51 | -0.54 | -0.58 | -0.45 | -0.53 | 0.13 | 0.13 | 0.11 | -0.11 | -0.05 | 0.24 | 0.27 | 0.32 | 0.35 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.5 | 0.3 | -0.6 | 0.1 | -1.0 | 0 | -1.5 | -1.2 | -1.0 | 3.9 | 3.8 | 5.0 | 2.7 | 4.8 | 5.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -2.5 | 0.6 | -2.7 | -2.9 | -2.7 | -2.9 | -5.0 | -2.7 | -2.9 | -0.8 | -0.9 | -1.7 | 1.0 | 0.5 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.9 | 2.6 | 12.3 | 13.1 | 12.6 | 15.0 | 14.7 | 13.2 | 13.6 | -4.5 | -4.5 | -4.6 | -2.4 | -1.0 | -2.0 |
Nucleus | 1.0 | 0.6 | 0.9 | 1.4 | 2.2 | 1.8 | 2.8 | 1.9 | 0.7 | 0 | 0 | 0 | 2.0 | 3.6 | 0 |
Nuclear Periphery | 0.3 | -2.9 | -7.6 | -8.3 | -10.5 | -9.1 | -10.3 | -9.5 | -7.6 | -1.0 | -0.9 | -2.6 | -5.5 | -7.0 | -7.4 |
Nucleolus | 4.0 | 2.3 | 4.9 | 5.0 | 5.1 | 5.2 | 5.8 | 5.7 | 6.0 | -1.4 | -1.5 | -1.1 | -0.5 | 0.7 | 2.1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -3.0 | -1.5 | -6.0 | -7.3 | -8.3 | -6.8 | -8.8 | -7.4 | -8.6 | -0.6 | -0.2 | 0.8 | 1.8 | 1.3 | 2.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.6555 | 8.1644 | 7.3642 | 6.4257 | 7.4493 | 7.3111 | 9.9259 | 10.5533 | 9.6711 | 9.5766 | 9.3829 | 9.6202 | 7.869 | 10.1328 | 9.7656 | 9.6531 | 9.0591 | 8.8694 |
Actin | 0.0126 | 0.0014 | 0.0063 | 0.0016 | 0.0008 | 0.0001 | 0.0142 | 0.0004 | 0.0097 | 0.0001 | 0.002 | 0 | 0.138 | 0.0049 | 0.0004 | 0.0012 | 0.0004 | 0.017 |
Bud | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0015 | 0.0002 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0027 | 0 | 0.0026 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0013 | 0.0001 | 0 | 0 | 0 | 0 |
Bud Periphery | 0.0004 | 0 | 0.0002 | 0.0003 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.003 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0 |
Bud Site | 0.0024 | 0.0001 | 0.001 | 0.0006 | 0.0004 | 0 | 0.0004 | 0 | 0.0007 | 0 | 0.0001 | 0 | 0.0032 | 0.0006 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0005 | 0.0001 | 0 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0056 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0045 | 0 | 0.0003 | 0 | 0.0008 | 0 | 0.0025 | 0.0029 | 0.0001 | 0.0001 | 0 | 0.0004 |
Cytoplasmic Foci | 0.0135 | 0.0011 | 0.0039 | 0.003 | 0.0012 | 0 | 0.0067 | 0 | 0.0049 | 0 | 0.0049 | 0 | 0.0132 | 0.0227 | 0 | 0 | 0 | 0.0025 |
Eisosomes | 0.0002 | 0 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0015 | 0.0001 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0051 | 0.0003 | 0.0008 | 0.0014 | 0.0012 | 0.0002 | 0.0009 | 0.0011 | 0.001 | 0.0006 | 0.0029 | 0.0001 | 0.0142 | 0.0046 | 0.001 | 0.0042 | 0.0002 | 0.0003 |
Endosome | 0.0199 | 0.001 | 0.0058 | 0.0186 | 0.0056 | 0.0002 | 0.0032 | 0.0003 | 0.0025 | 0.0002 | 0.0081 | 0.0001 | 0.1 | 0.0186 | 0.0004 | 0.0007 | 0.0003 | 0.0013 |
Golgi | 0.0053 | 0.0048 | 0.0048 | 0.0042 | 0.0042 | 0 | 0.0014 | 0.0002 | 0.0027 | 0.0001 | 0.0046 | 0 | 0.0442 | 0.0044 | 0.0001 | 0.0002 | 0.0002 | 0.0008 |
Lipid Particles | 0.022 | 0.0107 | 0.009 | 0.0348 | 0.0035 | 0.0005 | 0.0048 | 0.0014 | 0.0199 | 0.0032 | 0.0607 | 0.0004 | 0.066 | 0.0149 | 0.0009 | 0.0006 | 0.0016 | 0.0015 |
Mitochondria | 0.0063 | 0.0028 | 0.0067 | 0.0245 | 0.0752 | 0.0004 | 0.008 | 0.0073 | 0.0029 | 0.0008 | 0.0496 | 0.0002 | 0.0344 | 0.0035 | 0.0018 | 0.0028 | 0.0025 | 0.0004 |
None | 0.002 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0 | 0.0078 | 0.0002 | 0.0004 | 0.0001 | 0.0006 | 0 | 0.0036 | 0.0032 | 0.0003 | 0.0004 | 0.0001 | 0.0018 |
Nuclear Periphery | 0.8067 | 0.8673 | 0.8904 | 0.8419 | 0.872 | 0.9604 | 0.8869 | 0.9385 | 0.8967 | 0.9529 | 0.8092 | 0.983 | 0.5206 | 0.7856 | 0.9317 | 0.9205 | 0.9587 | 0.9465 |
Nucleolus | 0.0028 | 0.0013 | 0.0014 | 0.0026 | 0.0007 | 0.001 | 0.0007 | 0.0002 | 0.0005 | 0.0003 | 0.0013 | 0.0002 | 0.0015 | 0.001 | 0.0003 | 0.0003 | 0.0026 | 0.0003 |
Nucleus | 0.0391 | 0.0701 | 0.026 | 0.0198 | 0.0028 | 0.0222 | 0.0114 | 0.0036 | 0.0065 | 0.0056 | 0.0126 | 0.0044 | 0.0111 | 0.019 | 0.0148 | 0.0055 | 0.0066 | 0.0018 |
Peroxisomes | 0.0074 | 0.005 | 0.0033 | 0.0013 | 0.0018 | 0 | 0.0103 | 0 | 0.0085 | 0 | 0.0019 | 0 | 0.0048 | 0.007 | 0 | 0 | 0 | 0.0027 |
Punctate Nuclear | 0.0212 | 0.0006 | 0.0037 | 0.0013 | 0.0007 | 0.0005 | 0.01 | 0.0002 | 0.0019 | 0.0004 | 0.004 | 0.0004 | 0.0161 | 0.0535 | 0.0003 | 0.0004 | 0.0011 | 0.0051 |
Vacuole | 0.0023 | 0.0008 | 0.0006 | 0.0092 | 0.0012 | 0.0002 | 0.0004 | 0.0005 | 0.0007 | 0.0002 | 0.0013 | 0 | 0.0048 | 0.001 | 0.0006 | 0.0009 | 0.0003 | 0.0001 |
Vacuole Periphery | 0.0222 | 0.0323 | 0.0324 | 0.034 | 0.0275 | 0.0143 | 0.0279 | 0.0459 | 0.0394 | 0.0353 | 0.0349 | 0.0112 | 0.014 | 0.0515 | 0.047 | 0.0616 | 0.0251 | 0.0176 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 38.5421 | 33.6886 | 34.5757 | 43.4757 | 40.6117 | 31.3057 | 46.7254 | 41.7998 | 36.5861 | 48.996 |
Translational Efficiency | 0.4399 | 0.5532 | 0.5802 | 0.3799 | 0.3932 | 0.4635 | 0.4438 | 0.3571 | 0.3776 | 0.3602 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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