Standard name
Human Ortholog
Description Dolichol-P-Man dependent alpha(1-3) mannosyltransferase; involved in synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins; G353A missense mutation in human ortholog ALG3 implicated in carbohydrate deficient glycoprotein syndrome type IV, which is characterized by microcephaly, severe epilepsy, minimal psychomotor development, partial deficiency of sialic acids in serum glycoproteins; wild-type human ALG3 can complement yeast alg3 mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.59 0.7 0.74 0.88 0.91 0.87 0.86 0.93 0.9 0.91 0.93 0.92 0.88 0.91 0.97 0.59 0.54 0.58 0.07 0.11 0.09 0.14 0.17 0.15
Endoplasmic Reticulum 0 0.07 0.06 0 0 0 0 0 0 0 0 0 0.12 0.07 0 0 0 0 0.13 0.09 0.09 0.16 0.13 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.17 0.08 0 0 0.06 0.07 0 0.1 0.11 0.14 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.36 0.17 0.17 0.1 0.1 0.07 0.12 0.07 0.05 0.06 0 0 0 0 0 0.47 0.49 0.46 0.76 0.68 0.73 0.66 0.64 0.64
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 2 0 0 1 1 0 0 0 0 0 0 1 0 0 4
Bud 0 3 0 0 2 2 6 10 10 12 8 5 2 3 2 0 0 0 0 2 1 1 3 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 3 4 2 2 0 1 1 4 6 0 1 0 0 0 0 0 0 0
Cytoplasm 137 89 90 145 251 246 380 534 341 409 252 370 169 298 422 178 125 76 18 18 12 22 38 40
Endoplasmic Reticulum 4 9 7 3 6 10 5 3 10 5 0 4 23 24 10 6 10 2 35 14 12 26 27 18
Endosome 7 0 0 0 0 1 0 4 0 0 0 0 0 0 2 8 7 0 2 5 2 1 3 6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 2 0 0 0 2
Mitochondria 30 22 10 5 4 18 33 18 39 48 38 51 1 1 0 10 4 3 2 5 4 1 1 7
Nucleus 0 0 0 0 0 2 1 4 3 1 0 0 0 0 0 21 13 8 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 2 1 1 1 1 1 3 0 0 1 0 0 0 1 0 0 1 0 0
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 2
Vac/Vac Membrane 83 22 21 17 28 21 52 40 20 29 8 15 2 7 4 143 113 61 210 111 105 109 141 177
Unique Cell Count 231 128 122 165 277 283 444 576 379 448 272 401 193 328 436 302 230 132 278 163 144 166 221 278
Labelled Cell Count 264 145 128 170 292 302 481 618 426 509 307 449 199 338 447 367 275 151 278 163 144 166 221 278


Cytoplasm, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 4.7 5.1 4.9 5.3 4.8 4.4 4.6 4.5 4.1 4.0 4.1 5.8 5.1 4.9 7.8 9.7 11.0 5.5 5.3 5.6
Std Deviation (1e-4) 0.7 0.9 1.1 0.9 1.1 1.1 1.1 1.0 1.1 1.0 0.9 1.1 1.0 1.0 1.1 1.5 2.0 2.2 1.2 1.2 1.7
Intensity Change (Log2) – – – -0.05 0.05 -0.07 -0.19 -0.13 -0.18 -0.32 -0.34 -0.32 0.19 0.01 -0.04 0.62 0.93 1.11 0.13 0.06 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 5.8 5.9 4.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.1239 4.3338 4.676 4.6374 3.6659 4.4571 0.3742 0.6519 1.5371 1.24 1.2185 1.4895 2.2538 3.1196 2.619 2.6686 2.9348 2.8836
Actin 0.0002 0.0017 0.0043 0.0008 0.0001 0.0001 0.0091 0.0026 0.0048 0.0001 0.0001 0.0018 0.0204 0.0001 0.0183 0.0025 0.0004 0.0033
Bud 0.0001 0.0042 0.0112 0.0004 0.0005 0.0002 0.0001 0.0001 0.0003 0.0001 0.0001 0.0002 0.0004 0.0001 0.005 0.0006 0.0002 0.0007
Bud Neck 0.0002 0.0048 0.0012 0.0006 0.0023 0.0058 0.0009 0.0003 0.002 0.0006 0.0005 0.0009 0.0025 0.0003 0.0008 0.0004 0.0018 0.002
Bud Periphery 0.0003 0.0013 0.0044 0.0007 0.0011 0.0005 0.0004 0.0002 0.0004 0.0005 0.0002 0.0004 0.0008 0.0003 0.0013 0.0007 0.0003 0.001
Bud Site 0.0003 0.0111 0.0021 0.001 0.0029 0.0012 0.0007 0.0015 0.0033 0.0006 0.001 0.0006 0.0038 0.001 0.005 0.0007 0.0017 0.0028
Cell Periphery 0.0029 0.0025 0.0037 0.0008 0.005 0.0016 0.0098 0.0018 0.0021 0.0091 0.0019 0.0012 0.0018 0.0008 0.0015 0.0007 0.0007 0.0012
Cytoplasm 0.3255 0.4258 0.4547 0.2846 0.434 0.4141 0.2915 0.2741 0.2725 0.2825 0.3604 0.2854 0.3063 0.414 0.4123 0.3424 0.2947 0.3895
Cytoplasmic Foci 0.008 0.0195 0.0132 0.0319 0.0239 0.0184 0.0106 0.0217 0.0389 0.0118 0.008 0.0257 0.0254 0.019 0.0216 0.0302 0.0142 0.016
Eisosomes 0.0001 0.0002 0.0001 0.0001 0 0 0.0005 0.0014 0.0002 0 0 0 0.0003 0 0.0002 0.0002 0 0.0001
Endoplasmic Reticulum 0.1816 0.1291 0.1595 0.0813 0.0771 0.1357 0.1132 0.0937 0.1389 0.2737 0.0847 0.1958 0.2091 0.1702 0.1882 0.1003 0.2564 0.2226
Endosome 0.0263 0.074 0.0736 0.2578 0.1111 0.1084 0.0235 0.0543 0.1589 0.061 0.0745 0.1151 0.0622 0.0736 0.0925 0.1285 0.0799 0.0761
Golgi 0.0009 0.0017 0.0017 0.0069 0.0011 0.0013 0.0023 0.0012 0.0089 0.0016 0.0012 0.0033 0.0054 0.001 0.0041 0.0019 0.0012 0.0029
Lipid Particles 0.0219 0.0064 0.0088 0.006 0.0061 0.0099 0.0051 0.0139 0.0196 0.0096 0.0036 0.0318 0.0212 0.0074 0.0127 0.0083 0.0055 0.01
Mitochondria 0.0007 0.0013 0.0038 0.0149 0.0016 0.0016 0.0005 0.0006 0.0039 0.0007 0.0004 0.0009 0.002 0.0006 0.0032 0.0019 0.0005 0.0013
None 0.344 0.1523 0.1067 0.1636 0.0175 0.1088 0.3109 0.4162 0.182 0.0484 0.2842 0.1261 0.2143 0.2042 0.0825 0.293 0.1416 0.1101
Nuclear Periphery 0.0141 0.0196 0.0243 0.0146 0.0177 0.0166 0.0224 0.0163 0.0207 0.0353 0.0068 0.0143 0.0298 0.0167 0.0216 0.0146 0.0329 0.0212
Nucleolus 0.0002 0.001 0.0006 0.0006 0.001 0.0011 0.0004 0.0003 0.0005 0.0002 0.0002 0.0004 0.001 0.0003 0.0005 0.0006 0.001 0.0006
Nucleus 0.0043 0.0108 0.0066 0.0079 0.0131 0.0205 0.0049 0.0031 0.0048 0.0034 0.0033 0.0041 0.0132 0.0058 0.0049 0.0045 0.0356 0.0053
Peroxisomes 0.0001 0.0002 0.0002 0.0003 0.0002 0.0001 0.0002 0.0014 0.0009 0.0001 0.0001 0.0119 0.0049 0.0001 0.0018 0.0005 0.0002 0.0015
Punctate Nuclear 0.0006 0.0027 0.001 0.0014 0.0008 0.0012 0.0034 0.0013 0.004 0.0005 0.0003 0.0011 0.0043 0.001 0.0018 0.0049 0.0048 0.0016
Vacuole 0.0653 0.1243 0.1138 0.1144 0.2723 0.1442 0.1837 0.0919 0.1245 0.2549 0.1653 0.1711 0.0673 0.0793 0.1138 0.0568 0.1207 0.1238
Vacuole Periphery 0.0023 0.0053 0.0046 0.0092 0.0107 0.0086 0.0061 0.002 0.0081 0.0052 0.003 0.0077 0.0037 0.0041 0.0064 0.0058 0.0056 0.0065

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 51.6845 55.1568 38.271 42.7985 31.168 49.6537 56.1312 54.2607 51.2888 47.9695
Translational Efficiency 0.6261 0.6075 0.7651 0.6104 0.7074 0.5583 0.6321 0.551 0.5121 0.4523

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Dolichol-P-Man dependent alpha(1-3) mannosyltransferase; involved in synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins; G353A missense mutation in human ortholog ALG3 implicated in carbohydrate deficient glycoprotein syndrome type IV, which is characterized by microcephaly, severe epilepsy, minimal psychomotor development, partial deficiency of sialic acids in serum glycoproteins; wild-type human ALG3 can complement yeast alg3 mutant
Localization
Cell Percentages ER (20%), vacuole (17%), mixed (43%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Alg3

Alg3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Alg3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available