Standard name | |
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Human Ortholog | |
Description | Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 2 |
Bud | 1 | 0 | 1 | 1 | 2 | 2 | 5 | 1 | 4 | – | 14 | 4 | 2 | 0 | 2 | 0 | 0 | 1 | 2 | 4 | 1 | 3 | 6 | 9 |
Bud Neck | 0 | 0 | 13 | 3 | 13 | 10 | 25 | 19 | 11 | – | 6 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 2 | 2 | 4 | 6 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 3 | – | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 71 | 66 | 101 | 40 | 75 | 109 | 201 | 271 | 201 | – | 146 | 149 | 72 | 82 | 128 | 24 | 25 | 17 | 55 | 62 | 77 | 26 | 88 | 88 |
Endoplasmic Reticulum | 2 | 3 | 2 | 1 | 0 | 0 | 2 | 0 | 1 | – | 0 | 0 | 0 | 0 | 1 | 7 | 6 | 7 | 2 | 0 | 3 | 1 | 7 | 5 |
Endosome | 6 | 9 | 2 | 3 | 4 | 4 | 1 | 1 | 0 | – | 0 | 0 | 8 | 21 | 21 | 24 | 13 | 8 | 13 | 12 | 9 | 2 | 11 | 7 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 1 | 0 | 1 | 0 | 2 | 1 |
Mitochondria | 34 | 30 | 6 | 22 | 3 | 55 | 79 | 46 | 136 | – | 118 | 100 | 50 | 12 | 13 | 5 | 5 | 12 | 15 | 17 | 22 | 13 | 66 | 29 |
Nucleus | 20 | 38 | 16 | 19 | 28 | 25 | 24 | 22 | 14 | – | 13 | 10 | 60 | 52 | 56 | 54 | 25 | 19 | 41 | 67 | 32 | 9 | 14 | 6 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 0 | 1 | 1 | 1 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 7 | 3 | 2 | 2 | 3 | 1 | 6 | 6 | 3 | 2 | 2 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 1 | 5 | 8 | 34 | 20 | 39 | 23 | 15 | – | 11 | 9 | 6 | 2 | 1 | 7 | 6 | 1 | 38 | 31 | 30 | 17 | 42 | 26 |
Vac/Vac Membrane | 41 | 72 | 9 | 2 | 5 | 9 | 6 | 1 | 3 | – | 4 | 2 | 48 | 45 | 41 | 40 | 22 | 26 | 38 | 56 | 56 | 41 | 71 | 57 |
Unique Cell Count | 153 | 199 | 149 | 90 | 146 | 209 | 326 | 359 | 332 | 251 | 237 | 228 | 200 | 254 | 142 | 88 | 79 | 231 | 275 | 266 | 130 | 335 | 252 | |
Labelled Cell Count | 177 | 223 | 157 | 99 | 164 | 235 | 386 | 386 | 388 | 319 | 285 | 253 | 217 | 265 | 166 | 109 | 94 | 231 | 275 | 266 | 130 | 335 | 252 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.0 | 4.1 | 4.5 | 3.8 | 4.7 | 3.7 | 3.7 | 4.0 | 3.1 | – | 2.9 | 3.3 | 4.2 | 4.3 | 4.3 | 7.1 | 7.0 | 6.7 | 3.9 | 4.0 | 4.4 |
Std Deviation (1e-4) | 0.5 | 0.7 | 0.9 | 0.5 | 1.2 | 1.1 | 0.9 | 1.2 | 0.8 | – | 0.8 | 0.8 | 1.0 | 1.0 | 1.2 | 1.9 | 1.8 | 2.1 | 1.7 | 1.5 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.23 | 0.06 | -0.29 | -0.28 | -0.15 | -0.52 | – | -0.64 | -0.44 | -0.11 | -0.07 | -0.05 | 0.66 | 0.63 | 0.57 | -0.19 | -0.16 | -0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 5.0 | 4.2 | 5.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.9841 | -0.9425 | -0.7551 | -1.0731 | -0.9913 | -0.8585 | -0.0873 | -0.2366 | -0.2981 | -0.2832 | 0.2546 | -0.0379 | -0.5321 | -0.6151 | -0.6356 | -0.5258 | -0.3284 | -0.6503 |
Actin | 0.0331 | 0.0006 | 0.0251 | 0.0076 | 0.0129 | 0.008 | 0.0361 | 0.0004 | 0.0161 | 0.0003 | 0.01 | 0.0053 | 0.0489 | 0.0005 | 0.004 | 0.0163 | 0.0671 | 0.0009 |
Bud | 0.0008 | 0.0015 | 0.0008 | 0.0013 | 0.0007 | 0.0002 | 0.0019 | 0.0002 | 0.0003 | 0 | 0.0003 | 0.0011 | 0.0014 | 0.0003 | 0.0002 | 0.0002 | 0.0042 | 0.0001 |
Bud Neck | 0.0086 | 0.0007 | 0.0018 | 0.0015 | 0.0026 | 0.0056 | 0.0027 | 0.0018 | 0.0013 | 0.0006 | 0.0015 | 0.0157 | 0.0157 | 0.0012 | 0.0034 | 0.0006 | 0.0017 | 0.0057 |
Bud Periphery | 0.0013 | 0.0004 | 0.001 | 0.0033 | 0.0006 | 0.0004 | 0.0039 | 0.0001 | 0.0003 | 0 | 0.0005 | 0.0022 | 0.0018 | 0.0003 | 0.0003 | 0.0002 | 0.0074 | 0.0002 |
Bud Site | 0.0067 | 0.0028 | 0.0043 | 0.0024 | 0.0132 | 0.0019 | 0.0099 | 0.0024 | 0.0011 | 0.0001 | 0.0012 | 0.0017 | 0.0197 | 0.0028 | 0.0121 | 0.0004 | 0.0194 | 0.0003 |
Cell Periphery | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0008 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0008 | 0.0002 | 0.0005 | 0 | 0.0007 | 0.0001 |
Cytoplasm | 0.0814 | 0.0961 | 0.0667 | 0.0123 | 0.0678 | 0.0618 | 0.0822 | 0.0718 | 0.0463 | 0.0306 | 0.0569 | 0.056 | 0.0815 | 0.1002 | 0.0665 | 0.089 | 0.1209 | 0.0896 |
Cytoplasmic Foci | 0.0498 | 0.0139 | 0.014 | 0.0139 | 0.0146 | 0.0174 | 0.0408 | 0.0068 | 0.0111 | 0.0006 | 0.0434 | 0.0155 | 0.0578 | 0.0193 | 0.0146 | 0.0115 | 0.0127 | 0.0049 |
Eisosomes | 0.0008 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0008 | 0 |
Endoplasmic Reticulum | 0.0119 | 0.0057 | 0.0073 | 0.0021 | 0.0077 | 0.005 | 0.0123 | 0.005 | 0.0026 | 0.0011 | 0.0084 | 0.0018 | 0.0052 | 0.0083 | 0.0075 | 0.004 | 0.0052 | 0.0035 |
Endosome | 0.04 | 0.014 | 0.0128 | 0.0288 | 0.0259 | 0.0185 | 0.054 | 0.0059 | 0.0163 | 0.0009 | 0.0413 | 0.0107 | 0.0231 | 0.0059 | 0.0176 | 0.0043 | 0.035 | 0.0071 |
Golgi | 0.0094 | 0.0004 | 0.0039 | 0.0037 | 0.0083 | 0.0045 | 0.0174 | 0.0004 | 0.0117 | 0 | 0.0229 | 0.0038 | 0.0063 | 0.0003 | 0.0046 | 0.002 | 0.0259 | 0.0013 |
Lipid Particles | 0.0418 | 0.0015 | 0.0063 | 0.0092 | 0.0029 | 0.0112 | 0.0258 | 0.0012 | 0.0122 | 0.0001 | 0.0719 | 0.0033 | 0.0218 | 0.0014 | 0.0147 | 0.0023 | 0.0152 | 0.0129 |
Mitochondria | 0.0097 | 0.0008 | 0.0068 | 0.0076 | 0.0033 | 0.0138 | 0.0156 | 0.0006 | 0.0106 | 0.0003 | 0.0499 | 0.0049 | 0.0023 | 0.0006 | 0.0028 | 0.0014 | 0.0944 | 0.0009 |
None | 0.2063 | 0.1592 | 0.1718 | 0.0129 | 0.0382 | 0.1192 | 0.1182 | 0.1269 | 0.1179 | 0.1165 | 0.0408 | 0.0563 | 0.1721 | 0.2675 | 0.2078 | 0.121 | 0.076 | 0.1285 |
Nuclear Periphery | 0.0906 | 0.044 | 0.0503 | 0.0192 | 0.0722 | 0.0456 | 0.069 | 0.0477 | 0.0584 | 0.0641 | 0.0318 | 0.0232 | 0.0303 | 0.0463 | 0.0444 | 0.1304 | 0.0142 | 0.0248 |
Nucleolus | 0.0111 | 0.0163 | 0.0133 | 0.0159 | 0.0266 | 0.0296 | 0.0155 | 0.0368 | 0.0187 | 0.0085 | 0.0173 | 0.0317 | 0.0178 | 0.0287 | 0.0147 | 0.0056 | 0.0514 | 0.0326 |
Nucleus | 0.2377 | 0.5158 | 0.4734 | 0.6477 | 0.5937 | 0.4861 | 0.3724 | 0.5666 | 0.5331 | 0.7493 | 0.4931 | 0.6409 | 0.2833 | 0.3976 | 0.445 | 0.4946 | 0.3372 | 0.5998 |
Peroxisomes | 0.0202 | 0.0008 | 0.003 | 0.0027 | 0.001 | 0.0091 | 0.017 | 0.0017 | 0.0055 | 0 | 0.0279 | 0.0018 | 0.0332 | 0.0004 | 0.0077 | 0.0379 | 0.0414 | 0.001 |
Punctate Nuclear | 0.1247 | 0.1215 | 0.1334 | 0.2017 | 0.1026 | 0.1578 | 0.0827 | 0.1205 | 0.1302 | 0.0259 | 0.075 | 0.1214 | 0.1692 | 0.1133 | 0.1275 | 0.0764 | 0.0605 | 0.0836 |
Vacuole | 0.01 | 0.003 | 0.0022 | 0.0036 | 0.0027 | 0.0022 | 0.0161 | 0.0024 | 0.0042 | 0.0003 | 0.0032 | 0.0019 | 0.0061 | 0.0041 | 0.0027 | 0.0007 | 0.0064 | 0.0016 |
Vacuole Periphery | 0.0036 | 0.0007 | 0.0013 | 0.0024 | 0.0021 | 0.0016 | 0.0054 | 0.0007 | 0.0018 | 0.0007 | 0.0023 | 0.0006 | 0.0012 | 0.0007 | 0.0008 | 0.0013 | 0.0025 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.4494 | 12.9957 | 5.4646 | 18.8075 | 5.6546 | 7.2648 | 13.797 | 14.0419 | 10.1845 | 16.8748 |
Translational Efficiency | 0.5661 | 0.4277 | 1.0942 | 0.2594 | 0.747 | 0.8988 | 0.5478 | 0.417 | 0.7595 | 0.4339 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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