Standard name
Human Ortholog
Description Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.48 0.4 0.22 0.21 0.14 0.09 0.06 0.05 0 0 0 0 0.38 0.34 0.31 0.29 0.27 0.33 0.37 0.29 0.24 0.13 0.22 0.3
Bud 0.11 0.13 0.16 0.19 0.21 0.17 0.12 0.13 0.08 0.13 0.13 0.1 0.19 0.15 0.14 0.09 0.08 0.09 0.16 0.2 0.14 0.33 0.34 0.2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.24 0.23 0.21 0.4 0.31 0.24
Bud Site 0.13 0.16 0.13 0.17 0.1 0.13 0.07 0.06 0.06 0 0 0 0.06 0 0 0.05 0 0.08 – – – – – –
Cell Periphery 0.12 0.05 0.06 0.06 0 0 0 0 0 0 0.05 0 0.19 0.25 0.41 0.18 0.2 0.27 0.06 0 0.07 0 0 0
Cytoplasm 0.36 0.39 0.51 0.38 0.49 0.53 0.57 0.54 0.57 0.56 0.56 0.57 0.44 0.51 0.57 0.37 0.35 0.31 0.09 0.15 0.16 0.07 0.05 0.15
Endoplasmic Reticulum 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0.06 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Mitochondria 0 0.05 0.07 0.25 0.12 0.26 0.29 0.35 0.43 0.39 0.47 0.47 0 0 0.05 0 0 0.06 0 0 0.07 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
SpindlePole 0.14 0.08 0.12 0.09 0.14 0.07 0.09 0.06 0.05 0 0 0.05 0 0 0 0.18 0.18 0.13 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 98 80 53 50 46 31 24 22 6 5 5 6 50 66 69 111 58 72 94 74 76 7 45 61
Bud 23 25 38 46 68 55 52 60 21 49 34 27 25 29 32 34 18 20 40 50 43 19 68 41
Bud Neck 0 2 10 8 9 10 8 18 5 4 5 5 0 0 0 1 7 0 60 59 65 23 63 49
Bud Site 27 31 32 41 33 43 30 28 15 12 8 8 8 6 2 21 3 17 – – – – – –
Cell Periphery 24 10 14 14 7 13 10 8 10 11 14 8 25 48 91 70 42 59 16 4 22 0 3 6
Cytoplasm 74 78 125 90 162 175 237 256 145 215 144 150 59 99 128 143 74 68 24 38 49 4 9 30
Endoplasmic Reticulum 8 15 3 7 8 1 4 0 2 1 1 1 5 10 3 25 12 10 6 5 5 0 3 1
Endosome 0 3 2 1 4 0 0 2 0 0 0 0 0 0 0 7 3 2 1 4 8 0 1 1
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 4 10 5 0 0 0 0 2 0
Mitochondria 3 9 16 59 38 85 122 164 108 147 121 122 3 3 11 8 4 14 6 6 20 1 3 5
Nucleus 2 0 4 5 3 7 7 13 8 2 5 2 0 1 0 9 5 11 0 1 0 1 0 0
Nuclear Periphery 0 1 0 0 0 0 0 3 2 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 1 1 0 0 3 3 3 6 0 2 1 1 0 0 0 1 1 0 0 0 0 0 0 0
Peroxisomes 7 10 2 7 0 6 7 3 0 3 1 5 3 5 2 15 11 7 0 1 1 0 0 0
SpindlePole 28 16 29 22 45 23 38 30 13 14 8 13 4 5 3 70 39 28 0 0 4 0 0 1
Vac/Vac Membrane 5 1 4 3 11 1 3 6 0 2 2 5 0 1 2 29 14 11 1 7 9 0 0 1
Unique Cell Count 206 199 243 236 329 333 419 473 254 381 257 262 133 195 224 388 212 217 257 258 314 60 205 205
Labelled Cell Count 300 282 333 353 437 453 545 619 335 467 349 353 182 273 343 548 301 324 257 258 314 60 205 205


Bud, Bud Neck, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 6.2 5.9 4.6 5.3 4.0 3.7 3.7 3.2 3.1 3.1 2.9 6.4 6.4 5.6 8.8 9.5 9.5 – 7.4 7.8
Std Deviation (1e-4) 1.3 1.1 4.5 2.0 3.4 1.4 1.5 1.5 1.3 1.1 1.0 1.0 1.1 1.2 1.0 1.3 1.6 1.9 – 1.2 1.3
Intensity Change (Log2) – – – -0.35 -0.16 -0.58 -0.68 -0.66 -0.9 -0.92 -0.94 -1.01 0.12 0.1 -0.07 0.58 0.68 0.68 – 0.31 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -2.4 0 0 0 0 0 0 0 3.3 2.8 2.2 1.9 1.4 2.7
Bud 0 1.5 0 0 0 0 0 0 0 0.8 -0.2 -0.4 -2.6 -2.3 -2.1
Bud Neck 0 -0.9 0 0 0 0 0 0 0 0 0 0 0 -0.5 0
Bud Site 0 -1.2 0 0 0 0 0 0 0 -2.2 -3.7 -5.1 -3.4 -4.7 -1.9
Cell Periphery 0 -2.3 0 0 0 0 0 0 0 4.0 5.6 9.0 4.4 4.6 6.3
Cytoplasm -2.9 -0.5 0.3 1.3 0.7 1.3 1.2 1.0 1.3 -1.3 -0.1 1.2 -3.6 -3.5 -4.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 2.4 0 3.1 2.6 2.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 3.0 0
Mitochondria 0 2.0 0 0 0 0 0 0 0 -1.8 -2.6 -0.8 -2.9 -2.4 -0.1
Nucleus 0 0 0 0 0.9 1.1 0 0 0 0 0 0 0.6 0 2.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 2.3 2.8 0
SpindlePole 0 0.6 0 0 0 0 0 0 0 -2.9 -3.6 -4.5 2.1 1.9 0.3
Vacuole 0 1.3 0 0 0 0 0 0 0 0 0 0 3.2 2.7 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0711 3.8399 3.3939 3.0726 3.3387 3.7738 3.8039 5.7608 5.3869 5.447 4.8133 5.5551 5.0502 7.5081 6.7144 6.8957 7.0689 7.1777
Actin 0.188 0.0886 0.1682 0.1555 0.1849 0.0691 0.1056 0.0313 0.1143 0.1122 0.2275 0.0714 0.0908 0.1019 0.1345 0.3096 0.2587 0.1467
Bud 0.0249 0.0134 0.0996 0.0593 0.0294 0.0026 0.0183 0.0347 0.0814 0.0372 0.0241 0.0034 0.0319 0.0269 0.1143 0.0544 0.0234 0.0081
Bud Neck 0.0664 0.0103 0.0627 0.1467 0.1811 0.7372 0.0749 0.0137 0.0614 0.1044 0.1731 0.7181 0.086 0.0175 0.0556 0.1045 0.1683 0.618
Bud Periphery 0.0639 0.0237 0.2643 0.3081 0.1167 0.0092 0.0531 0.0583 0.241 0.1873 0.1262 0.0181 0.1126 0.0389 0.2713 0.2486 0.1276 0.0298
Bud Site 0.3018 0.487 0.0941 0.0129 0.0478 0.0137 0.2315 0.4067 0.156 0.057 0.063 0.0124 0.3793 0.5313 0.175 0.0752 0.0658 0.0114
Cell Periphery 0.0453 0.0165 0.0213 0.027 0.0583 0.0125 0.0503 0.0227 0.0271 0.0254 0.0272 0.0213 0.071 0.0286 0.0291 0.0753 0.0839 0.0226
Cytoplasm 0.1155 0.3221 0.2313 0.2301 0.2164 0.123 0.2149 0.3301 0.2284 0.3337 0.226 0.0927 0.1588 0.2265 0.1743 0.0778 0.2011 0.1247
Cytoplasmic Foci 0.0502 0.005 0.007 0.0029 0.0261 0.0092 0.0307 0.0098 0.0096 0.0194 0.0076 0.0076 0.0088 0.0032 0.0031 0.0051 0.0071 0.0116
Eisosomes 0.0029 0.0009 0.0007 0.0005 0.0028 0.0003 0.0033 0.0016 0.001 0.0005 0.0019 0.0017 0.0019 0.0004 0.0013 0.0047 0.0049 0.0004
Endoplasmic Reticulum 0.0137 0.0022 0.0038 0.006 0.0075 0.001 0.0093 0.0085 0.0046 0.0042 0.0094 0.0043 0.0019 0.0013 0.0013 0.0027 0.0033 0.0004
Endosome 0.0127 0.0009 0.0041 0.0044 0.0067 0.0041 0.02 0.0044 0.0106 0.0132 0.005 0.0048 0.001 0.0029 0.0007 0.0013 0.0029 0.0007
Golgi 0.0077 0.0012 0.0035 0.0021 0.0067 0.002 0.0077 0.0019 0.0059 0.009 0.0053 0.0027 0.0014 0.0014 0.0014 0.002 0.0049 0.0024
Lipid Particles 0.0118 0.001 0.0014 0.0113 0.0069 0.0011 0.0149 0.0022 0.0049 0.0078 0.0019 0.0016 0.001 0.0004 0.0003 0.0011 0.0006 0.0003
Mitochondria 0.0048 0.0002 0.0029 0.0005 0.0016 0.0029 0.0072 0.0008 0.0052 0.0035 0.0011 0.0011 0.0007 0.0004 0.0008 0.0015 0.001 0.0006
None 0.0471 0.0208 0.0222 0.0021 0.0939 0.0017 0.0904 0.0527 0.0346 0.0637 0.0846 0.0248 0.0419 0.0142 0.0318 0.0245 0.0348 0.0152
Nuclear Periphery 0.0033 0.0003 0.001 0.0006 0.0011 0.0029 0.0072 0.0056 0.002 0.0008 0.002 0.0017 0.0004 0.0003 0.0006 0.0006 0.0011 0.0001
Nucleolus 0.0018 0.0002 0.0005 0.0002 0.0009 0.0002 0.0065 0.0014 0.0006 0.0005 0.0011 0.0009 0.0006 0.0002 0.0002 0.0014 0.0011 0.0001
Nucleus 0.0051 0.0007 0.0015 0.001 0.0018 0.0006 0.0107 0.0046 0.0014 0.0014 0.0033 0.0019 0.0008 0.0009 0.0013 0.0016 0.0031 0.0003
Peroxisomes 0.0228 0.0002 0.0015 0.0002 0.0019 0.0049 0.007 0.0006 0.0034 0.0119 0.0018 0.002 0.0007 0.0003 0.0004 0.0013 0.0006 0.0056
Punctate Nuclear 0.0045 0.0003 0.0014 0.0002 0.0018 0.0005 0.0274 0.0042 0.0023 0.0015 0.0057 0.0063 0.0026 0.0003 0.0005 0.0009 0.0033 0.0002
Vacuole 0.0053 0.0044 0.0061 0.0266 0.0052 0.0012 0.0079 0.0039 0.0035 0.0052 0.0018 0.0011 0.0057 0.0022 0.002 0.0056 0.0022 0.0008
Vacuole Periphery 0.0008 0.0001 0.0008 0.0019 0.0005 0.0003 0.001 0.0004 0.0008 0.0005 0.0003 0.0002 0.0002 0.0001 0.0002 0.0004 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.6203 25.137 18.404 21.9786 28.9234 25.1061 27.0257 24.2444 21.6097 32.9491
Translational Efficiency 1.0384 0.9305 0.7869 0.5994 0.7507 1.007 0.8342 0.8375 0.8021 0.7504

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (23%), bud neck (13%), mixed (30%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Boi1

Boi1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Boi1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available