ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0.3 0.29 0.11 0.2 0.18 0.05 0.07 0.11 0.09 0.2 0.08 0.4 0.37 0.16 0.35 0.38 0.45 0.39 0.34 0.44 0.29 0.25 0.2
Bud – 0.07 0.09 0.11 0.1 0.12 0.08 0.1 0.11 0.13 0.1 0.08 0.08 0.07 0 0.08 0 0.07 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.22 0.17 0.09 0.08 0.06 0.07
Bud Site – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0.26 0.12 0.18 0.16 0.12 0.09 0.12 0.16 0.17 0.16 0.15 0.42 0.49 0.58 0.54 0.65 0.53 0.14 0.23 0.24 0.31 0.34 0.33
Cytoplasm – 0.44 0.4 0.5 0.41 0.2 0.2 0.26 0.22 0.16 0.18 0.12 0.4 0.27 0.33 0.21 0.11 0.12 0 0.05 0 0.08 0.08 0.11
Endoplasmic Reticulum – 0.11 0 0 0.1 0.17 0.09 0.06 0.06 0.06 0.09 0.06 0.05 0.05 0 0 0.06 0.06 0.09 0.06 0.05 0.11 0.14 0.12
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.09 0 0 0 0 0 0
Mitochondria – 0.1 0.25 0.28 0.2 0.56 0.67 0.56 0.69 0.79 0.78 0.82 0 0 0 0.11 0.11 0.09 0.05 0 0.09 0.08 0.06 0.06
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0.09 0.07 0.1 0.07 0.07 0.1 0.05 0.05 0 0 0.08 0.18 0.27 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 46 35 12 22 30 12 19 26 24 37 17 90 145 65 105 102 88 15 68 69 52 83 61
Bud – 11 11 12 11 20 20 27 25 34 18 16 19 27 17 25 5 14 0 1 0 2 2 6
Bud Neck – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 33 14 14 21 22
Bud Site – 1 6 3 1 2 4 8 6 9 1 2 1 1 0 2 2 1 – – – – – –
Cell Periphery – 40 14 20 18 20 22 35 38 45 29 31 94 194 240 160 174 105 5 46 38 55 113 104
Cytoplasm – 67 48 55 46 33 50 73 52 42 33 24 91 108 137 63 30 24 1 10 2 14 26 33
Endoplasmic Reticulum – 17 5 3 11 29 22 16 15 15 16 12 12 20 17 12 17 11 3 12 7 19 46 38
Endosome – 0 2 2 0 0 3 9 0 0 0 0 4 2 24 3 0 0 1 5 4 0 0 2
Golgi – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 6 29 17 0 3 1 0 0 1
Mitochondria – 15 30 31 22 93 164 158 161 208 145 166 6 14 18 33 29 17 1 8 13 13 21 19
Nucleus – 0 0 0 1 0 1 2 0 1 3 1 0 1 0 5 3 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 1 0 2 1 1 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus – 0 0 0 0 0 2 0 1 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0
Peroxisomes – 0 11 8 11 11 18 27 12 14 7 7 17 71 112 12 9 3 0 1 1 0 0 1
SpindlePole – 0 5 7 2 0 4 8 3 5 1 2 6 4 16 10 4 6 0 0 0 0 1 0
Vac/Vac Membrane – 0 0 1 0 1 1 3 1 2 1 1 0 0 6 4 4 0 0 5 1 4 8 11
Unique Cell Count 151 120 109 111 166 244 281 233 262 187 202 226 393 416 296 269 197 39 199 157 179 332 314
Labelled Cell Count 197 167 155 145 239 323 386 341 401 292 280 340 588 652 441 408 287 39 199 157 179 332 314


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 5.5 4.7 5.0 5.6 4.6 4.3 4.8 4.8 4.2 4.7 4.3 5.8 6.1 6.3 8.1 10.5 10.8 5.1 5.3 6.2
Std Deviation (1e-4) – 0.9 1.4 1.7 2.1 1.1 1.2 1.4 1.6 1.1 1.7 1.1 1.0 1.2 1.2 1.4 1.8 1.9 1.4 1.5 1.8
Intensity Change (Log2) – – – 0.06 0.23 -0.03 -0.16 0.02 0.01 -0.17 -0.01 -0.14 0.3 0.36 0.41 0.77 1.15 1.19 0.12 0.15 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -3.4 -1.6 0 0 0 0 0 0 0 2.0 1.6 -3.4 1.2 1.7 2.7
Bud 0.5 0.2 0 0 0 0 0 0 0 -0.2 -0.8 -2.2 -0.2 0 -0.7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.4 1.0 0 0 0 0 0 0 0 5.7 7.4 8.9 7.9 9.7 7.4
Cytoplasm 1.6 0.2 -3.7 -3.9 -2.8 -3.5 -5.1 -4.3 -5.9 0 -2.6 -1.4 -3.9 -6.6 -5.7
Endoplasmic Reticulum 0 1.7 0 0 0 0 0 0 0 0.5 0.4 0 0 0.8 0.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 1.8 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 3.7 3.3
Mitochondria 0.6 -0.9 0 0 0 0 0 0 0 -6.5 -7.3 -7.0 -3.6 -3.6 -4.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -0.5 0.2 0 0 0 0 0 0 0 -0.5 2.3 4.1 -2.1 -2.4 -3.2
SpindlePole 0.8 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5186 1.5688 0.6855 0.9765 0.3887 0.868 2.6159 2.9458 3.1111 2.7793 2.6452 2.7153 3.9691 4.6583 4.5778 4.149 3.242 3.4531
Actin 0.1989 0.1505 0.1625 0.2608 0.2264 0.2129 0.1504 0.089 0.0939 0.0815 0.2725 0.1538 0.1622 0.0751 0.1197 0.1364 0.2088 0.1877
Bud 0.0029 0.002 0.0024 0.0027 0.0018 0.0026 0.0015 0.0013 0.0014 0.0019 0.0034 0.0014 0.005 0.0079 0.0015 0.0235 0.0014 0.0138
Bud Neck 0.0303 0.0369 0.1798 0.3117 0.0822 0.1854 0.0291 0.0207 0.1656 0.2659 0.0936 0.1471 0.0288 0.0298 0.0997 0.2229 0.1411 0.0999
Bud Periphery 0.008 0.0054 0.014 0.016 0.011 0.0207 0.0077 0.006 0.0076 0.0057 0.0109 0.018 0.0095 0.0098 0.0081 0.0363 0.0116 0.0131
Bud Site 0.1008 0.369 0.1318 0.0466 0.0543 0.0123 0.1307 0.4232 0.1924 0.055 0.0187 0.0093 0.1061 0.3392 0.105 0.0518 0.0177 0.0066
Cell Periphery 0.1017 0.0778 0.097 0.0759 0.1614 0.1242 0.2797 0.132 0.1513 0.1192 0.2492 0.2303 0.1933 0.0781 0.1496 0.1731 0.1714 0.1288
Cytoplasm 0.1746 0.2031 0.154 0.1943 0.2079 0.238 0.1723 0.2577 0.1503 0.1424 0.1796 0.3342 0.241 0.2541 0.2862 0.1949 0.3062 0.3689
Cytoplasmic Foci 0.0537 0.0211 0.0296 0.0101 0.0416 0.0359 0.0088 0.0054 0.0335 0.0552 0.0266 0.0089 0.0223 0.0131 0.0134 0.0051 0.017 0.0184
Eisosomes 0.0326 0.0107 0.0178 0.0149 0.0605 0.0136 0.0312 0.0087 0.0272 0.0047 0.0249 0.0214 0.0381 0.0106 0.0133 0.0089 0.0159 0.0185
Endoplasmic Reticulum 0.0142 0.0103 0.0105 0.0036 0.0066 0.0063 0.004 0.0042 0.0031 0.0047 0.0047 0.0027 0.0162 0.0037 0.0051 0.0026 0.0058 0.0049
Endosome 0.0142 0.0028 0.0062 0.0019 0.0016 0.0045 0.0062 0.0004 0.0091 0.0482 0.0223 0.0006 0.0147 0.0047 0.0029 0.0006 0.0072 0.001
Golgi 0.0171 0.0088 0.0136 0.0059 0.0048 0.0074 0.0058 0.0025 0.0126 0.0319 0.054 0.0024 0.0092 0.0031 0.0097 0.0023 0.0194 0.0048
Lipid Particles 0.0264 0.0078 0.0063 0.0018 0.004 0.0087 0.0043 0.002 0.0063 0.042 0.008 0.001 0.0096 0.0022 0.0036 0.001 0.0151 0.0033
Mitochondria 0.0113 0.0051 0.0061 0.0022 0.0538 0.0052 0.0177 0.0001 0.0102 0.0086 0.018 0.0004 0.0087 0.0089 0.0101 0.0245 0.0082 0.0022
None 0.1783 0.0817 0.1549 0.0468 0.0749 0.1061 0.1424 0.0434 0.1072 0.1 0.0021 0.0661 0.1008 0.1521 0.1643 0.1047 0.0429 0.1205
Nuclear Periphery 0.0029 0.0006 0.0007 0.0003 0.0003 0.0004 0.0009 0.0003 0.0012 0.0033 0.0015 0.0001 0.0126 0.0003 0.0003 0.0004 0.0007 0.0002
Nucleolus 0.0024 0.0005 0.0011 0.0004 0.0003 0.0007 0.0007 0.0005 0.001 0.0008 0.0002 0.0001 0.0014 0.0005 0.0006 0.0019 0.0003 0.0005
Nucleus 0.0027 0.0006 0.0013 0.0007 0.0004 0.0011 0.0005 0.0006 0.0018 0.0011 0.0005 0.0002 0.0031 0.0009 0.0007 0.0042 0.0004 0.0011
Peroxisomes 0.0168 0.0019 0.0045 0.0014 0.004 0.0104 0.0024 0.0002 0.0189 0.0194 0.0063 0.0009 0.0034 0.0035 0.0037 0.0013 0.006 0.0035
Punctate Nuclear 0.0058 0.0017 0.0034 0.0006 0.0009 0.0015 0.0006 0.0003 0.0041 0.0046 0.0006 0.0001 0.0056 0.0003 0.0004 0.0004 0.0006 0.0004
Vacuole 0.0037 0.0014 0.0021 0.0012 0.0008 0.002 0.0022 0.0017 0.0012 0.0028 0.0016 0.0009 0.0043 0.0019 0.0019 0.0025 0.0019 0.0018
Vacuole Periphery 0.0007 0.0001 0.0003 0.0001 0.0001 0.0002 0.0008 0 0.0002 0.0011 0.0007 0.0001 0.004 0.0003 0.0001 0.001 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.6324 3.5203 5.7468 9.5876 9.7008 12.5944 7.1187 8.4081 9.9065 12.3941
Translational Efficiency 1.1483 2.2309 0.9993 0.9279 1.2569 1.2801 1.0779 1.0147 1.068 0.8599

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
Localization
Cell Percentages cell periphery (15%), cytoplasm (3%), mixed (33%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YBL086C

YBL086C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ybl086c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available