Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 |
Bud | 0 | 1 | 0 | 0 | 0 | 1 | 1 | – | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 3 | 2 | 0 | 2 | 6 | 3 |
Bud Neck | 0 | 0 | 0 | 1 | 0 | 1 | 0 | – | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 1 | 0 | 0 | 2 | 2 | 1 | 0 | – | 1 | 8 | 6 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 12 | 3 | 5 | 10 | 7 | 9 | – | 1 | 7 | 4 | 14 | 21 | 48 | 68 | 12 | 3 | 6 | 0 | 1 | 1 | 1 | 1 | 2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 |
Endosome | 1 | 4 | 0 | 1 | 0 | 0 | 2 | – | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 0 | 1 | 3 | 7 | 5 | 1 | 1 | 4 | 5 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 0 | 1 | 1 | 0 | 2 |
Mitochondria | 6 | 31 | 0 | 1 | 1 | 56 | 7 | – | 34 | 20 | 22 | 24 | 0 | 20 | 0 | 2 | 1 | 4 | 2 | 4 | 2 | 1 | 3 | 4 |
Nucleus | 373 | 501 | 239 | 164 | 250 | 219 | 271 | – | 219 | 212 | 195 | 223 | 182 | 333 | 271 | 91 | 84 | 90 | 370 | 537 | 219 | 189 | 273 | 252 |
Nuclear Periphery | 2 | 2 | 0 | 1 | 2 | 2 | 1 | – | 3 | 4 | 1 | 0 | 2 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Nucleolus | 144 | 71 | 33 | 22 | 28 | 74 | 51 | – | 104 | 83 | 76 | 108 | 28 | 76 | 46 | 16 | 14 | 9 | 84 | 38 | 42 | 81 | 104 | 138 |
Peroxisomes | 1 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 7 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 4 | 6 | 0 | 0 | 0 | 4 | – | 1 | 0 | 1 | 2 | 2 | 4 | 8 | 1 | 0 | 0 | 3 | 4 | 1 | 1 | 5 | 10 |
Vac/Vac Membrane | 5 | 5 | 1 | 1 | 3 | 2 | 2 | – | 0 | 1 | 2 | 0 | 1 | 2 | 3 | 2 | 1 | 7 | 10 | 18 | 6 | 11 | 23 | 28 |
Unique Cell Count | 455 | 558 | 255 | 181 | 286 | 271 | 311 | 266 | 274 | 243 | 301 | 211 | 426 | 382 | 112 | 94 | 115 | 492 | 617 | 279 | 297 | 433 | 454 | |
Labelled Cell Count | 542 | 633 | 282 | 199 | 297 | 363 | 348 | 364 | 337 | 307 | 377 | 237 | 489 | 412 | 128 | 105 | 125 | 492 | 617 | 279 | 297 | 433 | 454 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.3 | 7.5 | 8.1 | 7.8 | 8.4 | 5.6 | 7.2 | – | 6.4 | 6.7 | 6.7 | 6.7 | 9.1 | 7.7 | 9.3 | 11.0 | 11.6 | 11.8 | 7.8 | 8.7 | 9.0 |
Std Deviation (1e-4) | 2.2 | 1.6 | 1.5 | 1.5 | 1.7 | 1.5 | 1.5 | – | 1.4 | 1.5 | 1.5 | 1.6 | 2.5 | 2.6 | 2.8 | 2.8 | 2.6 | 2.4 | 1.9 | 1.7 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.05 | -0.53 | -0.17 | – | -0.35 | -0.27 | -0.26 | -0.27 | 0.17 | -0.08 | 0.2 | 0.44 | 0.51 | 0.54 | -0.05 | 0.1 | 0.15 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 1.8 | 0 | 1.4 | – | 0 | 0 | 0 | 2.4 | 4.3 | 4.8 | 6.5 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 7.7 | 0 | – | 5.9 | 4.4 | 4.9 | 4.6 | 0 | 3.5 | 0 | 0 | 0 | 0 |
Nucleus | -1.2 | -2.5 | -4.4 | -2.6 | – | -4.0 | -5.3 | -4.5 | -6.2 | -2.7 | -5.4 | -7.1 | -3.7 | -1.4 | -4.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.2 | -1.2 | 4.1 | 1.2 | – | 6.8 | 4.8 | 4.9 | 6.2 | 0.1 | 1.7 | -0.3 | 0.3 | 0.5 | -1.4 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | -0.2 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.1513 | 2.2593 | 1.7698 | 1.4441 | 1.3277 | 2.0219 | 3.935 | 3.9355 | 3.908 | 3.3634 | 3.9954 | 3.8006 | 7.4738 | 8.2516 | 7.3008 | 6.9649 | 6.8536 | 6.9288 |
Actin | 0.0062 | 0 | 0.0017 | 0.0013 | 0.0001 | 0 | 0.0293 | 0 | 0.0123 | 0.0041 | 0.0217 | 0.0004 | 0.001 | 0 | 0 | 0 | 0.0002 | 0 |
Bud | 0.001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0005 | 0 | 0.0002 | 0.0004 | 0.0016 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Bud Neck | 0.0019 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0011 | 0.0017 | 0.0001 | 0.0006 | 0.0004 | 0.0025 | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0009 | 0.0003 |
Bud Periphery | 0.0034 | 0.0002 | 0.0007 | 0.0003 | 0.0002 | 0.0001 | 0.0014 | 0 | 0.0002 | 0.0011 | 0.0049 | 0.0012 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Bud Site | 0.0041 | 0.0002 | 0.0001 | 0.0011 | 0.0002 | 0.0001 | 0.0018 | 0 | 0.0034 | 0.0008 | 0.0027 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0004 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0015 | 0 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0067 | 0.0001 | 0.0006 | 0.0007 | 0.0015 | 0.0001 | 0.0019 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0035 | 0 | 0.0001 | 0.0087 | 0 | 0 | 0.01 | 0 | 0.0027 | 0.0008 | 0.0026 | 0.0004 | 0.0004 | 0 | 0 | 0 | 0 | 0 |
Eisosomes | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0008 | 0 | 0.0003 | 0.0002 | 0 | 0 | 0.0073 | 0 | 0.0007 | 0.0039 | 0.0033 | 0.0001 | 0.0016 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0104 | 0 | 0.0009 | 0.0153 | 0.0001 | 0.0001 | 0.02 | 0 | 0.0032 | 0.0087 | 0.0102 | 0.0018 | 0.006 | 0 | 0 | 0 | 0.0001 | 0 |
Golgi | 0.0039 | 0 | 0.0003 | 0.005 | 0 | 0 | 0.0033 | 0 | 0.0021 | 0.0024 | 0.0108 | 0.0009 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Lipid Particles | 0.0089 | 0 | 0.0007 | 0.0224 | 0 | 0 | 0.0119 | 0 | 0.0056 | 0.0037 | 0.032 | 0.0097 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.0168 | 0.0002 | 0.0079 | 0.0076 | 0.0006 | 0.0004 | 0.0023 | 0.0001 | 0.0048 | 0.03 | 0.0495 | 0.007 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 |
None | 0.0032 | 0 | 0.003 | 0.0008 | 0.0001 | 0 | 0.0026 | 0 | 0.0008 | 0.0004 | 0.0007 | 0.0001 | 0.0011 | 0 | 0 | 0 | 0.0001 | 0 |
Nuclear Periphery | 0.015 | 0.0004 | 0.0052 | 0.0019 | 0.0012 | 0.001 | 0.0138 | 0.0007 | 0.0027 | 0.0129 | 0.0251 | 0.001 | 0.0131 | 0.0007 | 0.0003 | 0.0003 | 0.0023 | 0.0001 |
Nucleolus | 0.0412 | 0.0272 | 0.03 | 0.0143 | 0.1756 | 0.108 | 0.05 | 0.0189 | 0.0199 | 0.0127 | 0.2249 | 0.0688 | 0.0246 | 0.0186 | 0.0199 | 0.0136 | 0.1266 | 0.0602 |
Nucleus | 0.8544 | 0.9668 | 0.9452 | 0.902 | 0.8186 | 0.8883 | 0.8244 | 0.9793 | 0.9317 | 0.9065 | 0.5659 | 0.9048 | 0.9469 | 0.9799 | 0.9793 | 0.9858 | 0.8607 | 0.9376 |
Peroxisomes | 0.0036 | 0 | 0.0002 | 0.0117 | 0 | 0 | 0.001 | 0 | 0.0049 | 0.0004 | 0.0033 | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0118 | 0.0042 | 0.0009 | 0.0057 | 0.0023 | 0.0005 | 0.0085 | 0.0008 | 0.0029 | 0.0055 | 0.0303 | 0.0007 | 0.001 | 0.0003 | 0.0003 | 0.0002 | 0.0078 | 0.0015 |
Vacuole | 0.0045 | 0.0003 | 0.0009 | 0.0005 | 0.0002 | 0.0001 | 0.0019 | 0 | 0.0004 | 0.0023 | 0.003 | 0.0006 | 0.0005 | 0 | 0 | 0 | 0.0001 | 0 |
Vacuole Periphery | 0.003 | 0.0001 | 0.0009 | 0.0006 | 0.0001 | 0.0001 | 0.0009 | 0 | 0.0002 | 0.0018 | 0.0026 | 0.0005 | 0.0006 | 0 | 0 | 0 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 43.2708 | 38.6814 | 36.4311 | 40.2206 | 33.4457 | 28.2768 | 42.8714 | 37.5651 | 38.9524 | 42.1167 |
Translational Efficiency | 1.698 | 1.773 | 1.5849 | 1.2335 | 1.7238 | 2.496 | 1.329 | 1.934 | 1.6134 | 1.6313 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|