Standard name
Human Ortholog
Description Protein involved in regulating endocytosis of plasma membrane proteins; identified as a substrate for ubiquitination by Rsp5p and deubiquitination by Ubp2p; promoter contains several Gcn4p binding elements; ECM21 has a paralog, CSR2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.05 0 0.07 0.06 0.06 0 0 0 0 0.09 0.08 0.11 0.19 0.27 0.39 0.15 0.11 0.13 0 0 0 0 0 0
Cytoplasm 0.96 0.96 0.99 0.99 0.96 0.93 0.89 0.87 0.88 0.79 0.76 0.71 0.99 0.98 0.98 0.91 0.88 0.95 0.97 0.93 0.93 0.93 0.92 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.09 0.06 0.16 0.22 0.2 0.2 0.35 0.38 0.45 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 2 0
Bud 0 0 6 3 2 5 11 5 5 3 5 9 1 0 2 0 0 1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cell Periphery 4 9 14 8 10 8 13 12 12 20 11 19 33 51 71 18 10 14 0 0 0 0 0 0
Cytoplasm 77 281 213 132 167 214 301 261 261 181 110 125 171 185 180 112 82 105 91 291 226 97 129 175
Endoplasmic Reticulum 2 5 0 1 1 2 1 3 2 5 0 3 1 2 1 2 3 2 0 1 2 2 3 3
Endosome 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0
Mitochondria 0 6 2 12 10 38 74 59 59 80 55 79 0 0 0 3 0 2 0 4 2 0 0 0
Nucleus 0 4 0 1 0 1 3 1 1 2 4 2 1 1 0 4 1 2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 1 0 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 2 0 0 1 0 1 4 1 0 1 1 0 0 0 6 1 4 0 5 3 0 0 1
Unique Cell Count 80 294 215 134 174 231 338 299 298 229 145 175 173 189 183 123 93 110 95 313 243 105 140 186
Labelled Cell Count 83 307 235 158 191 269 408 347 343 291 188 240 207 239 254 145 98 130 95 313 243 105 140 186


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.2 7.8 5.6 5.5 5.5 5.1 4.7 4.7 4.7 4.1 4.2 4.0 8.9 8.9 8.0 13.7 14.2 15.2 7.9 9.4 10.1
Std Deviation (1e-4) 1.2 1.2 0.6 0.9 0.9 0.9 1.1 1.0 1.0 0.9 1.0 0.9 1.2 1.2 1.1 2.6 3.4 2.4 1.4 1.6 1.7
Intensity Change (Log2) -0.02 -0.01 -0.13 -0.25 -0.24 -0.24 -0.45 -0.42 -0.5 0.67 0.68 0.51 1.29 1.35 1.44 0.5 0.75 0.85


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 -0.4 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.2 -0.3 -1.5 0 0 0 0 0 0 3.8 5.6 7.8 2.5 1.3 1.9
Cytoplasm -0.5 -2.0 -3.4 -4.5 -4.9 -4.8 -6.7 -7.1 -8.0 -0.2 -1.0 -0.6 -3.7 -4.4 -2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.7 2.7 5.7 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.5264 5.8543 5.102 5.0423 4.1311 5.4014 6.6842 7.7093 7.4455 6.8011 7.4622 7.3904 7.2897 7.8938 7.4702 8.2628 6.6313 7.7576
Actin 0.1053 0.0046 0.0156 0.0586 0.0086 0.0048 0.0558 0.0044 0.0264 0.0426 0.0186 0.0059 0.0231 0.0033 0.0032 0.0044 0.0003 0.0168
Bud 0.0014 0.0003 0.0005 0.0009 0.002 0.0006 0.003 0.0096 0.0027 0.0019 0.0016 0.003 0.0005 0.0002 0.0004 0.0005 0.0002 0.0021
Bud Neck 0.003 0.0006 0.0009 0.0008 0.0039 0.0009 0.0054 0.0004 0.0034 0.0009 0.0012 0.0032 0.0007 0.0004 0.0005 0.0007 0.0006 0.0055
Bud Periphery 0.0023 0.0002 0.0008 0.0017 0.0013 0.0006 0.0043 0.0037 0.0026 0.0038 0.0014 0.0015 0.0008 0.0002 0.0004 0.0004 0.0001 0.002
Bud Site 0.0047 0.0187 0.0097 0.0006 0.0213 0.0006 0.0222 0.0229 0.0196 0.0039 0.0047 0.0012 0.0029 0.0066 0.0025 0.0006 0.0002 0.0005
Cell Periphery 0.0014 0.0024 0.0016 0.0014 0.0007 0.0001 0.0025 0.0021 0.0021 0.0009 0.0005 0.0009 0.0025 0.0019 0.0023 0.0009 0.0001 0.0007
Cytoplasm 0.6342 0.9555 0.934 0.9056 0.7681 0.9342 0.5449 0.9007 0.826 0.7972 0.6705 0.936 0.8029 0.9749 0.9437 0.9682 0.9788 0.9369
Cytoplasmic Foci 0.0453 0.0033 0.0033 0.0034 0.0358 0.0035 0.0255 0.0072 0.0098 0.0121 0.0513 0.0082 0.024 0.0029 0.0043 0.0044 0.0035 0.0065
Eisosomes 0.0041 0 0.0001 0.0003 0.0001 0 0.0005 0.0001 0.0015 0.0003 0.0002 0 0.0005 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0182 0.0054 0.0031 0.0048 0.0028 0.0032 0.0278 0.0073 0.0071 0.0043 0.0096 0.0065 0.009 0.0027 0.0033 0.0037 0.0035 0.0124
Endosome 0.0289 0.0019 0.0041 0.0053 0.0498 0.0187 0.0429 0.0067 0.022 0.035 0.0529 0.0031 0.0184 0.0008 0.0023 0.0029 0.0059 0.006
Golgi 0.0116 0.0009 0.0054 0.0035 0.0168 0.0052 0.0153 0.0018 0.0064 0.0142 0.0214 0.0011 0.0193 0.0006 0.0008 0.0005 0.0003 0.0047
Lipid Particles 0.0199 0.0003 0.0027 0.0003 0.0076 0.0007 0.0074 0.0009 0.0048 0.0023 0.0031 0.0004 0.0034 0.0003 0.0003 0.0003 0 0.0004
Mitochondria 0.0331 0.0001 0.0056 0.0018 0.0141 0.0109 0.0083 0.0005 0.0041 0.0138 0.0064 0.0005 0.0111 0 0.0001 0 0.0002 0.0005
None 0.0162 0.0038 0.007 0.0028 0.0296 0.0015 0.1195 0.0257 0.0351 0.037 0.0798 0.0234 0.0374 0.0037 0.0335 0.0088 0.0027 0.0031
Nuclear Periphery 0.0283 0.0003 0.0007 0.0019 0.0155 0.0018 0.0223 0.0008 0.0031 0.0025 0.0311 0.0005 0.017 0.0002 0.0003 0.0004 0.0007 0.0002
Nucleolus 0.0006 0 0.0002 0 0.0012 0.0001 0.002 0.0002 0.0005 0.0004 0.0003 0.0002 0.0003 0 0 0 0 0
Nucleus 0.0047 0.0004 0.0008 0.0009 0.0038 0.0009 0.0288 0.0013 0.0029 0.0021 0.0112 0.0014 0.0133 0.0004 0.0004 0.0005 0.0007 0.0003
Peroxisomes 0.0232 0 0.0007 0.0001 0.005 0.0008 0.0171 0.0002 0.0036 0.0009 0.0236 0.0002 0.0081 0 0.0001 0 0 0.0007
Punctate Nuclear 0.0073 0.0001 0.0008 0.0002 0.0047 0.0001 0.0287 0.0011 0.0009 0.0004 0.0053 0.0009 0.0014 0.0001 0.0002 0.0002 0.0001 0.0001
Vacuole 0.0027 0.001 0.0017 0.0045 0.0053 0.0058 0.0131 0.0021 0.012 0.0198 0.0031 0.0016 0.0023 0.0007 0.0013 0.0026 0.0015 0.0007
Vacuole Periphery 0.0038 0.0001 0.0006 0.0005 0.0023 0.0049 0.0026 0.0002 0.0033 0.0036 0.0023 0.0002 0.0012 0 0.0001 0.0001 0.0004 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.7781 15.5867 17.6764 24.2508 13.2227 19.3992 23.3423 29.803 29.5424 23.6994
Translational Efficiency 0.4009 0.4323 0.5717 0.4497 0.4556 0.6068 0.5423 0.4508 0.5273 0.4513

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein involved in regulating endocytosis of plasma membrane proteins; identified as a substrate for ubiquitination by Rsp5p and deubiquitination by Ubp2p; promoter contains several Gcn4p binding elements; ECM21 has a paralog, CSR2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Ecm21

Ecm21


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ecm21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available