Standard name
Human Ortholog
Description Subunit of SEACAT, a subcomplex of the SEA complex; Sea4p, along with Rtc1p and Mtc5p, redundantly inhibit the TORC1 inhibitory role of the Iml1p/SEACIT (Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p (EGOC subunit) in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamically with the vacuole; contains an N-terminal beta-propeller fold and a C-terminal RING motif
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.96 – 0.93 0.98 0.96 0.94 0.94 0.92 0.9 0.79 0.82 0.79 0.94 1.0 0.99 0.96 0.94 0.96 0.95 0.93 0.85 0.85 0.85 0.83
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0.06
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0.09 0.07 0 0.2 0.16 0.2 0.3 0.39 0.44 0.43 0.06 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 2 1
Bud 0 – 1 0 0 1 1 1 5 2 3 3 0 0 4 0 0 0 0 0 0 0 0 1
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 – 0 0 2 0 2 3 1 5 1 6 0 2 4 13 11 15 0 0 0 0 0 0
Cytoplasm 104 – 52 83 140 122 196 212 167 105 129 198 17 121 158 337 206 333 104 47 66 41 122 136
Endoplasmic Reticulum 1 – 0 0 0 0 1 4 0 2 1 2 0 0 0 15 12 12 0 0 1 1 3 9
Endosome 0 – 0 0 3 1 0 2 1 0 1 0 0 0 0 4 2 1 0 0 0 0 2 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 1 2
Mitochondria 3 – 5 6 4 26 34 45 56 52 70 108 1 0 6 8 1 6 0 0 2 1 0 3
Nucleus 0 – 0 1 0 0 0 1 1 1 1 0 0 0 0 1 1 1 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 1 0 1 0 1 0 1 2 0 0 0 8 8 10 0 1 4 0 4 3
Unique Cell Count 108 56 85 146 130 209 230 186 133 158 250 18 121 159 350 218 348 110 51 78 49 143 164
Labelled Cell Count 111 58 90 150 150 236 269 232 167 207 319 18 124 173 387 243 379 110 51 78 49 143 164


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 – 4.9 5.1 5.2 4.8 4.6 4.6 4.4 4.1 4.1 3.9 7.1 7.4 6.4 8.0 8.4 9.0 – 7.2 7.7
Std Deviation (1e-4) 1.0 – 0.7 0.9 0.7 1.3 1.0 0.9 1.3 0.8 1.1 1.2 0.9 1.2 0.9 1.4 1.9 1.7 – 1.5 1.4
Intensity Change (Log2) – – – 0.06 0.09 -0.03 -0.08 -0.1 -0.17 -0.25 -0.26 -0.33 0.54 0.6 0.39 0.71 0.78 0.88 – 0.55 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 0.9 0.3 0.3 -0.2 -0.7 -2.3 -2.0 -2.4 0.2 3.0 2.8 1.2 0.5 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6302 4.0164 3.2122 2.8125 2.7697 3.3364 3.4622 4.8581 4.3327 4.1959 3.9141 4.4496 1.1456 2.318 1.5618 1.7647 1.5337 1.5455
Actin 0.0431 0.0004 0.01 0.0475 0.0415 0.0043 0.0318 0.0001 0.0043 0.0001 0.0004 0.0021 0.0002 0.0003 0.0142 0.0019 0.0146 0.0029
Bud 0.0007 0.0002 0.0029 0.003 0.0078 0.0005 0.001 0.0002 0.0053 0.0016 0.0001 0.0005 0.0001 0.0001 0.0006 0.0003 0.0002 0.0001
Bud Neck 0.0028 0.0002 0.0011 0.0016 0.0029 0.0014 0.0014 0.0001 0.0004 0.0003 0.0014 0.0004 0.0001 0.0001 0.0119 0.0001 0.0002 0.0004
Bud Periphery 0.0007 0.0002 0.0021 0.0034 0.0099 0.0007 0.001 0.0001 0.0032 0.0014 0 0.0006 0 0 0.0004 0.0002 0.0003 0.0001
Bud Site 0.009 0.0011 0.0052 0.0007 0.0121 0.0002 0.0041 0.0005 0.0048 0.0005 0.0002 0.0012 0.0014 0.0007 0.0024 0.0002 0.0003 0.0001
Cell Periphery 0.0007 0.0006 0.0005 0.0003 0.0006 0.0003 0.0003 0.0001 0.0003 0.0001 0 0.0001 0.0004 0.0002 0.0003 0.0002 0.0002 0.0001
Cytoplasm 0.7332 0.9382 0.8688 0.8141 0.7073 0.9209 0.7143 0.9342 0.8706 0.8734 0.8235 0.9282 0.8483 0.9005 0.7539 0.6557 0.7737 0.8815
Cytoplasmic Foci 0.0364 0.0056 0.02 0.0185 0.0233 0.0089 0.0579 0.0068 0.0106 0.0096 0.0503 0.0077 0.0194 0.0036 0.0302 0.0345 0.0127 0.0081
Eisosomes 0.0006 0 0.0001 0.0002 0.0007 0 0.0006 0 0.0003 0 0 0 0 0 0.0003 0.0001 0.0002 0
Endoplasmic Reticulum 0.017 0.0049 0.0069 0.0042 0.0171 0.0101 0.0102 0.0018 0.0055 0.0022 0.0018 0.0046 0.0059 0.001 0.0078 0.0048 0.0054 0.0032
Endosome 0.0463 0.0089 0.0208 0.0574 0.0341 0.016 0.0613 0.0066 0.0214 0.0473 0.0099 0.0214 0.0112 0.0015 0.0433 0.0541 0.0182 0.0057
Golgi 0.0107 0.0007 0.0034 0.0098 0.0096 0.0016 0.0102 0.0003 0.0086 0.0018 0.003 0.004 0.0009 0.0002 0.0067 0.0027 0.0052 0.002
Lipid Particles 0.0137 0.0002 0.0026 0.0012 0.0141 0.0003 0.0139 0.0001 0.0006 0.0003 0.0005 0.0008 0.0005 0 0.0027 0.0031 0.0168 0.0032
Mitochondria 0.0061 0.0003 0.002 0.0011 0.065 0.0021 0.0264 0.0001 0.0363 0.0032 0.0003 0.0107 0.0002 0 0.0024 0.0007 0.0106 0.0057
None 0.0384 0.031 0.0363 0.0072 0.0297 0.0226 0.0154 0.0456 0.0189 0.0098 0.0178 0.0141 0.1061 0.0903 0.0952 0.2325 0.1149 0.0735
Nuclear Periphery 0.0054 0.0008 0.0028 0.0014 0.005 0.003 0.0096 0.0003 0.0016 0.0042 0.0081 0.0003 0.0007 0.0002 0.0015 0.0013 0.0012 0.0002
Nucleolus 0.0006 0 0.0003 0.0001 0.001 0.0001 0.0008 0 0.0001 0.0002 0.0003 0 0.0001 0 0.0001 0.0001 0.0001 0
Nucleus 0.0021 0.0008 0.0016 0.0018 0.0019 0.0009 0.0171 0.0006 0.001 0.0024 0.0572 0.0005 0.0006 0.0003 0.0008 0.0008 0.0005 0.0003
Peroxisomes 0.014 0 0.0023 0.0005 0.0067 0.0001 0.0096 0 0.001 0.0001 0.019 0.0004 0.0001 0 0.0175 0.0011 0.0208 0.0108
Punctate Nuclear 0.008 0.0001 0.0026 0.0004 0.0043 0.0002 0.0034 0.0001 0.0003 0.0001 0.0009 0.0001 0.0002 0.0001 0.0008 0.0007 0.0013 0.0001
Vacuole 0.0089 0.0052 0.0067 0.0225 0.0039 0.0036 0.0081 0.0023 0.0039 0.0292 0.0044 0.0016 0.0032 0.0007 0.0056 0.004 0.002 0.0016
Vacuole Periphery 0.0017 0.0005 0.0011 0.003 0.0016 0.002 0.0017 0.0002 0.0009 0.0122 0.001 0.0006 0.0003 0.0001 0.0011 0.0011 0.0006 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.9739 11.3922 13.3847 13.8663 14.2281 12.383 14.3983 12.7412 15.3871 16.5429
Translational Efficiency 0.6168 0.4703 0.4668 0.4438 0.382 0.5044 0.3922 0.3616 0.3311 0.3965

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of SEACAT, a subcomplex of the SEA complex; Sea4p, along with Rtc1p and Mtc5p, redundantly inhibit the TORC1 inhibitory role of the Iml1p/SEACIT (Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p (EGOC subunit) in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamically with the vacuole; contains an N-terminal beta-propeller fold and a C-terminal RING motif
Localization
Cell Percentages cytoplasm (92%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Sea4

Sea4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sea4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available