Standard name | |
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Human Ortholog | |
Description | Protein serine/threonine kinase; essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 128 | 2 | 32 | 56 | 59 | 51 | 47 | 40 | 16 | 22 | 19 | 8 | 9 | 36 | 35 | 25 | 27 | 19 | 85 | 4 | 14 | 10 | 32 | 43 |
Bud | 86 | 0 | 27 | 21 | 70 | 56 | 64 | 75 | 34 | 43 | 27 | 35 | 9 | 25 | 33 | 13 | 12 | 8 | 160 | 16 | 39 | 71 | 121 | 133 |
Bud Neck | 5 | 0 | 0 | 3 | 7 | 6 | 13 | 15 | 1 | 6 | 1 | 2 | 0 | 0 | 0 | 1 | 2 | 1 | 91 | 12 | 22 | 55 | 72 | 57 |
Bud Site | 57 | 3 | 12 | 33 | 60 | 51 | 38 | 23 | 16 | 22 | 13 | 11 | 2 | 2 | 2 | 3 | 3 | 4 | – | – | – | – | – | – |
Cell Periphery | 53 | 3 | 1 | 26 | 15 | 25 | 36 | 41 | 32 | 32 | 21 | 37 | 31 | 84 | 96 | 15 | 20 | 12 | 23 | 1 | 2 | 1 | 1 | 8 |
Cytoplasm | 213 | 16 | 50 | 41 | 77 | 74 | 108 | 150 | 85 | 110 | 56 | 97 | 163 | 213 | 242 | 80 | 96 | 71 | 124 | 24 | 29 | 12 | 55 | 55 |
Endoplasmic Reticulum | 8 | 1 | 1 | 1 | 1 | 3 | 4 | 10 | 5 | 4 | 4 | 2 | 3 | 7 | 2 | 2 | 10 | 3 | 5 | 0 | 0 | 0 | 1 | 3 |
Endosome | 5 | 0 | 0 | 1 | 8 | 3 | 1 | 10 | 1 | 1 | 0 | 1 | 2 | 0 | 2 | 1 | 6 | 4 | 2 | 1 | 3 | 1 | 4 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 3 | 3 | 30 | 57 | 38 | 71 | 122 | 200 | 120 | 149 | 86 | 140 | 1 | 1 | 21 | 9 | 0 | 6 | 4 | 0 | 4 | 2 | 1 | 4 |
Nucleus | 6 | 0 | 0 | 2 | 17 | 17 | 11 | 15 | 4 | 9 | 9 | 9 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 2 | 3 | 5 | 3 | 2 | 1 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 4 | 0 | 1 | 0 | 6 | 9 | 13 | 44 | 5 | 12 | 2 | 14 | 1 | 0 | 0 | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 14 | 1 | 3 | 4 | 11 | 9 | 7 | 9 | 4 | 6 | 0 | 1 | 1 | 0 | 2 | 1 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 25 | 0 | 0 | 3 | 28 | 20 | 19 | 27 | 3 | 5 | 3 | 5 | 0 | 0 | 0 | 5 | 9 | 14 | 4 | 0 | 2 | 3 | 4 | 2 |
Vac/Vac Membrane | 7 | 1 | 3 | 1 | 12 | 9 | 13 | 41 | 8 | 11 | 9 | 19 | 1 | 0 | 2 | 7 | 8 | 10 | 0 | 0 | 3 | 1 | 1 | 4 |
Unique Cell Count | 441 | 25 | 110 | 154 | 272 | 254 | 332 | 501 | 233 | 304 | 178 | 257 | 185 | 269 | 318 | 123 | 152 | 125 | 515 | 64 | 128 | 162 | 303 | 322 |
Labelled Cell Count | 615 | 30 | 160 | 249 | 409 | 406 | 499 | 705 | 337 | 434 | 251 | 391 | 223 | 368 | 437 | 165 | 200 | 160 | 515 | 64 | 128 | 162 | 303 | 322 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.6 | 7.2 | 5.0 | 5.0 | 5.5 | 5.1 | 4.8 | 4.9 | 4.4 | 4.4 | 4.6 | 4.2 | 8.1 | 7.9 | 6.7 | 9.8 | 10.3 | 11.2 | 7.8 | 7.4 | 7.7 |
Std Deviation (1e-4) | 1.7 | 1.3 | 1.0 | 1.0 | 1.2 | 1.7 | 1.4 | 1.2 | 1.5 | 1.2 | 1.3 | 1.1 | 1.6 | 1.5 | 1.2 | 1.9 | 2.0 | 2.2 | 1.6 | 1.7 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.14 | 0.04 | -0.06 | -0.02 | -0.17 | -0.17 | -0.13 | -0.24 | 0.7 | 0.66 | 0.42 | 0.98 | 1.05 | 1.17 | 0.64 | 0.57 | 0.63 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.2 | -1.5 | -1.9 | 0 | -6.2 | 0 | 0 | 0 | 0 | -5.8 | -3.6 | -4.5 | -1.6 | -2.2 | -2.6 |
Bud | -2.3 | 0.2 | -0.5 | 0 | -2.4 | 0 | 0 | 0 | 0 | -5.0 | -3.9 | -3.7 | -2.8 | -3.7 | -3.9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 2.2 | 2.5 | 2.1 | 0 | -2.6 | 0 | 0 | 0 | 0 | -3.8 | 0 | 0 | -2.6 | -3.1 | -2.3 |
Cell Periphery | 4.2 | 0 | 3.0 | 0 | 2.7 | 0 | 0 | 0 | 0 | 4.2 | 6.4 | 6.3 | 3.4 | 3.6 | 2.9 |
Cytoplasm | -3.2 | -3.2 | -3.0 | -2.5 | -3.1 | -1.6 | -1.7 | -2.4 | -1.4 | 7.9 | 6.5 | 6.0 | 3.0 | 2.8 | 1.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.7 | -3.1 | 0.1 | 0 | 2.5 | 0 | 0 | 0 | 0 | -7.2 | -8.7 | -5.8 | -4.1 | -6.8 | -4.8 |
Nucleus | 0 | 0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 3.5 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 |
Vacuole | 0 | 0 | 0 | 0 | 2.0 | 0 | 0 | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 1.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.2723 | 4.5553 | 4.2313 | 4.0979 | 3.9769 | 4.5626 | 6.0746 | 6.5938 | 6.2101 | 5.5624 | 5.71 | 6.566 | 3.2783 | 3.4973 | 3.2403 | 4.0345 | 3.2764 | 3.5323 |
Actin | 0.03 | 0.0163 | 0.035 | 0.0563 | 0.03 | 0.0155 | 0.0789 | 0.0076 | 0.0256 | 0.0434 | 0.0453 | 0.0138 | 0.0386 | 0.0104 | 0.0321 | 0.0229 | 0.0288 | 0.0165 |
Bud | 0.0109 | 0.0196 | 0.2207 | 0.1064 | 0.0816 | 0.0102 | 0.0431 | 0.0319 | 0.2133 | 0.1116 | 0.0432 | 0.0015 | 0.0331 | 0.0463 | 0.1817 | 0.0863 | 0.05 | 0.0068 |
Bud Neck | 0.105 | 0.0125 | 0.009 | 0.0011 | 0.0018 | 0.6872 | 0.0297 | 0.0059 | 0.0042 | 0.0049 | 0.1002 | 0.6738 | 0.1146 | 0.0095 | 0.0028 | 0.004 | 0.0386 | 0.5453 |
Bud Periphery | 0.0209 | 0.0162 | 0.2629 | 0.5163 | 0.3379 | 0.0176 | 0.057 | 0.02 | 0.2338 | 0.2337 | 0.081 | 0.0031 | 0.0715 | 0.0516 | 0.2607 | 0.4044 | 0.1265 | 0.0147 |
Bud Site | 0.1671 | 0.4153 | 0.126 | 0.0036 | 0.0039 | 0.0086 | 0.157 | 0.3521 | 0.0431 | 0.0111 | 0.0162 | 0.0066 | 0.1854 | 0.3746 | 0.0613 | 0.052 | 0.0093 | 0.0064 |
Cell Periphery | 0.0113 | 0.0101 | 0.0075 | 0.0098 | 0.0124 | 0.0019 | 0.0159 | 0.0034 | 0.0023 | 0.0034 | 0.0107 | 0.0022 | 0.0211 | 0.002 | 0.004 | 0.0071 | 0.0125 | 0.0017 |
Cytoplasm | 0.5739 | 0.4759 | 0.3083 | 0.2898 | 0.5125 | 0.2385 | 0.4888 | 0.5641 | 0.3972 | 0.308 | 0.5748 | 0.2527 | 0.4349 | 0.4798 | 0.3726 | 0.4069 | 0.5842 | 0.3771 |
Cytoplasmic Foci | 0.0106 | 0.0092 | 0.0061 | 0.0021 | 0.0013 | 0.0058 | 0.0231 | 0.0042 | 0.0115 | 0.0199 | 0.042 | 0.0109 | 0.0147 | 0.0032 | 0.0082 | 0.0013 | 0.0109 | 0.0183 |
Eisosomes | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0067 | 0.003 | 0.0043 | 0.0035 | 0.0057 | 0.0013 | 0.0064 | 0.0014 | 0.0026 | 0.0058 | 0.003 | 0.0012 | 0.0078 | 0.0014 | 0.0019 | 0.0047 | 0.0035 | 0.0011 |
Endosome | 0.0038 | 0.0016 | 0.0008 | 0.0024 | 0.0013 | 0.001 | 0.0307 | 0.0014 | 0.0179 | 0.0875 | 0.0461 | 0.0043 | 0.012 | 0.0026 | 0.0037 | 0.0013 | 0.0187 | 0.0016 |
Golgi | 0.0019 | 0.0008 | 0.0007 | 0.0029 | 0.0008 | 0.0009 | 0.0129 | 0.0009 | 0.0181 | 0.0294 | 0.0055 | 0.006 | 0.0092 | 0.0006 | 0.0044 | 0.0003 | 0.0136 | 0.0016 |
Lipid Particles | 0.0012 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0004 | 0.0081 | 0.0001 | 0.0036 | 0.0184 | 0.0036 | 0.0024 | 0.011 | 0.0001 | 0.0014 | 0.0003 | 0.0176 | 0.0005 |
Mitochondria | 0.0003 | 0.0002 | 0.0005 | 0.0012 | 0.0008 | 0.0002 | 0.0141 | 0.0008 | 0.0121 | 0.0928 | 0.001 | 0.0068 | 0.0152 | 0.0003 | 0.0236 | 0.0004 | 0.0082 | 0.0003 |
None | 0.0316 | 0.0124 | 0.0108 | 0.0007 | 0.001 | 0.0055 | 0.0048 | 0.0029 | 0.0045 | 0.0034 | 0.0032 | 0.0025 | 0.0067 | 0.0024 | 0.0329 | 0.0028 | 0.0659 | 0.0008 |
Nuclear Periphery | 0.0009 | 0.0004 | 0.0007 | 0.0007 | 0.0014 | 0.0003 | 0.0048 | 0.0004 | 0.0006 | 0.0037 | 0.0058 | 0.0002 | 0.0019 | 0.0006 | 0.0006 | 0.0006 | 0.0008 | 0.0001 |
Nucleolus | 0.001 | 0.0003 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0006 | 0.0003 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0.0005 | 0.0002 | 0.0002 |
Nucleus | 0.0015 | 0.0017 | 0.0013 | 0.0008 | 0.0014 | 0.001 | 0.0031 | 0.0012 | 0.0011 | 0.0024 | 0.003 | 0.0004 | 0.0012 | 0.0032 | 0.0014 | 0.001 | 0.0009 | 0.0005 |
Peroxisomes | 0.0013 | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0004 | 0.0103 | 0.0006 | 0.0051 | 0.009 | 0.0028 | 0.0102 | 0.0156 | 0.0001 | 0.0041 | 0.0001 | 0.0054 | 0.0056 |
Punctate Nuclear | 0.0015 | 0.0007 | 0.0009 | 0.0001 | 0.0001 | 0.0003 | 0.0033 | 0.0005 | 0.0003 | 0.0011 | 0.0088 | 0.0004 | 0.0019 | 0.0002 | 0.0004 | 0.0005 | 0.0009 | 0.0004 |
Vacuole | 0.017 | 0.0025 | 0.0027 | 0.0016 | 0.005 | 0.003 | 0.0057 | 0.0006 | 0.002 | 0.0068 | 0.0029 | 0.0004 | 0.0018 | 0.0102 | 0.0015 | 0.0023 | 0.0028 | 0.0007 |
Vacuole Periphery | 0.0012 | 0.0001 | 0.0002 | 0.0004 | 0.0008 | 0.0002 | 0.0012 | 0.0001 | 0.0008 | 0.0022 | 0.0005 | 0.0002 | 0.0009 | 0.0005 | 0.0003 | 0.0001 | 0.0004 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.8701 | 27.1139 | 27.5322 | 32.2406 | 23.3882 | 17.5502 | 26.6213 | 30.2868 | 34.4656 | 30.8759 |
Translational Efficiency | 0.5967 | 0.5221 | 0.6157 | 0.5552 | 0.4967 | 0.7618 | 0.5833 | 0.5547 | 0.4738 | 0.4797 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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