Standard name
Human Ortholog
Description Succinate semialdehyde dehydrogenase; involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0.05 0 0.05 0.05 0.08 0.08 0.07 0.07 0 0 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.98 0.99 0.93 0.89 0.78 0.7 0.7 0.69 0.69 0.69 1.0 0.99 0.99 0.97 0.94 0.96 0.94 0.89 0.93 0.87 0.92 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0.09 0.09 0.08 0.05 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0.05 0.07 0.08 0.06 0.16 0.13 0.15 0.18 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.08 0.15 0.08 0.13 0.14 0.11 0 0 0 0 0.08 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 8 8 6 3 4 0 0 0 0 0 1 0 2 2 1 2 3
Bud 0 2 0 0 3 2 2 2 2 5 2 4 0 0 0 0 0 0 1 4 2 2 2 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 6 4 3 13 15 14 19 27 27 20 25 0 3 4 5 6 12 0 0 0 0 0 0
Cytoplasm 201 360 299 160 241 314 230 284 241 243 211 238 419 365 395 187 161 186 191 334 277 132 229 180
Endoplasmic Reticulum 1 1 2 0 3 7 26 37 27 18 19 22 1 0 2 4 6 4 0 0 3 1 1 2
Endosome 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 2 3 1 1 0 1
Golgi 1 0 0 0 1 0 2 2 3 0 2 0 0 1 0 1 4 1 1 0 2 1 0 1
Mitochondria 12 5 4 3 12 26 23 25 56 44 47 61 0 0 0 1 0 2 3 9 3 2 4 3
Nucleus 0 2 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 2 0 2 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 1 1 2 8 4 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 1 1
Vac/Vac Membrane 1 6 3 1 6 7 23 60 29 46 42 38 0 2 3 5 13 1 0 4 2 1 0 1
Unique Cell Count 207 367 305 162 260 351 294 407 344 350 305 344 420 368 399 192 172 194 205 375 300 152 251 209
Labelled Cell Count 216 382 313 167 279 371 322 439 395 398 351 392 420 371 404 203 191 209 205 375 300 152 251 209


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.7 6.6 6.5 10.4 13.9 15.6 16.6 19.1 18.2 19.1 19.2 5.3 5.9 6.0 23.7 28.4 27.7 5.3 5.3 6.3
Std Deviation (1e-4) 1.0 1.7 2.8 2.2 7.5 4.8 6.2 7.0 9.6 8.9 9.7 10.2 1.1 1.5 1.5 7.8 9.2 7.8 2.1 1.4 2.0
Intensity Change (Log2) – – – -0.02 0.66 1.08 1.25 1.34 1.54 1.47 1.54 1.55 -0.32 -0.16 -0.13 1.85 2.11 2.07 -0.32 -0.32 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2.6 2.3 2.5 2.5 3.9 3.8 3.3 3.7 0 0 0 0 0 3.0
Cytoplasm 0.6 -3.1 -4.4 -7.5 -9.7 -9.5 -9.7 -9.6 -9.7 2.4 1.3 1.1 -0.5 -2.5 -1.4
Endoplasmic Reticulum 0 0 0 4.7 4.9 4.4 3.3 3.8 3.9 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.4 3.7 3.8 3.2 6.6 5.5 6.3 7.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 4.1 6.4 4.4 5.9 6.0 5.3 0 0 0 0 3.8 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.81 2.6051 2.1516 1.9291 1.891 2.0575 3.5184 3.5088 3.671 3.2946 3.1474 3.6289 4.9156 5.8077 4.793 4.5711 4.59 4.6673
Actin 0.0395 0.0057 0.0124 0.0112 0.037 0.0267 0.0337 0.0059 0.0347 0.0612 0.0045 0.0233 0.0269 0.0021 0.0337 0.0002 0.0124 0.006
Bud 0.0013 0.006 0.0009 0.001 0.004 0.0018 0.0062 0.0003 0.0054 0.0039 0.0071 0.0017 0.0027 0.0063 0.0006 0.0005 0.0012 0.0002
Bud Neck 0.0098 0.0003 0.0014 0.0007 0.0071 0.0028 0.0024 0.0002 0.0009 0.0109 0.0003 0.006 0.0087 0.0002 0.0003 0.0001 0.0022 0.0003
Bud Periphery 0.002 0.0034 0.0017 0.001 0.02 0.0051 0.0055 0.0003 0.0067 0.0056 0.0065 0.0036 0.003 0.0033 0.0009 0.0006 0.0026 0.0004
Bud Site 0.011 0.0045 0.0038 0.0014 0.0014 0.0013 0.0173 0.0026 0.0079 0.0155 0.0008 0.0007 0.0081 0.0026 0.0008 0.0001 0.0064 0.0001
Cell Periphery 0.0006 0.0005 0.0006 0.0002 0.0003 0.0002 0.0007 0.0003 0.0004 0.0004 0.0001 0.0001 0.0004 0.0006 0.0002 0 0.0003 0.0001
Cytoplasm 0.4684 0.7051 0.5761 0.5875 0.567 0.6435 0.399 0.4943 0.4076 0.4547 0.4883 0.4592 0.4948 0.5526 0.5329 0.7374 0.4763 0.6092
Cytoplasmic Foci 0.0188 0.009 0.0158 0.0269 0.0172 0.0289 0.0429 0.0196 0.0307 0.0146 0.0072 0.0215 0.0633 0.0058 0.0207 0.0064 0.0149 0.0061
Eisosomes 0.0009 0.0001 0.0003 0.0003 0.0003 0.0003 0.0003 0.0004 0.0002 0.0003 0.0001 0.0002 0.0002 0.0001 0.0002 0 0.0001 0.0001
Endoplasmic Reticulum 0.0073 0.004 0.0053 0.0061 0.0045 0.0039 0.0155 0.0027 0.0049 0.0038 0.0011 0.0016 0.0055 0.0025 0.0092 0.0008 0.0108 0.0083
Endosome 0.0241 0.004 0.0201 0.0322 0.0199 0.0167 0.0314 0.0132 0.018 0.006 0.0053 0.0077 0.039 0.0006 0.0124 0.0012 0.062 0.0118
Golgi 0.0124 0.0003 0.0093 0.0116 0.0077 0.0198 0.014 0.0015 0.0123 0.0096 0.0057 0.0041 0.0229 0.0003 0.0062 0.0001 0.0099 0.0024
Lipid Particles 0.0139 0.0003 0.0093 0.0077 0.0191 0.0143 0.0134 0.0018 0.0092 0.0007 0.0053 0.0032 0.0318 0.0001 0.0121 0 0.0078 0.001
Mitochondria 0.0184 0.0004 0.0086 0.0165 0.0031 0.0215 0.0051 0.0004 0.0071 0.0242 0.0109 0.0047 0.0226 0.0003 0.0061 0.0002 0.0127 0.0032
None 0.3328 0.2466 0.3101 0.2737 0.2699 0.186 0.3589 0.4495 0.4096 0.3775 0.446 0.4469 0.19 0.4179 0.3444 0.2496 0.3166 0.345
Nuclear Periphery 0.0078 0.0016 0.0049 0.0032 0.0033 0.0017 0.0136 0.0007 0.0066 0.0018 0.0005 0.0008 0.0079 0.0006 0.0016 0.0003 0.0255 0.003
Nucleolus 0.0023 0.0001 0.0004 0.0003 0.0004 0.0001 0.0011 0.0002 0.0005 0.0001 0.0001 0.0001 0.0009 0.0001 0.0001 0 0.0009 0
Nucleus 0.006 0.003 0.0041 0.0023 0.0022 0.0051 0.0041 0.0009 0.003 0.0023 0.001 0.0017 0.0028 0.0011 0.0014 0.0011 0.0061 0.001
Peroxisomes 0.012 0.0008 0.009 0.0087 0.0073 0.0166 0.0198 0.0019 0.0239 0.0043 0.0073 0.0098 0.023 0.0016 0.0094 0.0001 0.0032 0.0001
Punctate Nuclear 0.0054 0.001 0.0012 0.0033 0.006 0.0016 0.0059 0.0021 0.0078 0.0014 0.0005 0.0025 0.04 0.0003 0.0051 0.0003 0.0089 0.0004
Vacuole 0.0039 0.0028 0.0038 0.0034 0.0016 0.0016 0.0062 0.0012 0.0019 0.0008 0.0011 0.0005 0.0032 0.0009 0.0011 0.0007 0.0098 0.0008
Vacuole Periphery 0.0015 0.0004 0.0009 0.0009 0.0006 0.0005 0.0031 0.0002 0.0008 0.0003 0.0003 0.0002 0.0021 0.0001 0.0004 0.0001 0.0093 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.9705 5.2818 6.8588 8.3605 6.703 8.1196 8.0106 10.1374 12.112 10.4446
Translational Efficiency 1.2545 1.0137 1.2949 1.4444 1.198 2.1047 1.1533 0.9404 1.1666 1.0309

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Succinate semialdehyde dehydrogenase; involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm
Localization
Cell Percentages cytoplasm (80%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Uga2

Uga2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Uga2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available