Standard name | |
---|---|
Human Ortholog | |
Description | Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 6 | 4 | 8 | 6 | 11 | 3 | 7 | – | 3 | 5 | 4 | 2 | 7 | 10 | 4 | 1 | 2 | 2 | 2 | 5 | 7 | 16 | 31 | 21 |
Bud | 7 | 8 | 5 | 6 | 10 | 6 | 15 | – | 14 | 26 | 17 | 16 | 12 | 9 | 16 | 5 | 2 | 4 | 2 | 10 | 8 | 11 | 18 | 20 |
Bud Neck | 0 | 1 | 7 | 2 | 3 | 6 | 3 | – | 4 | 0 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 5 | 7 | 23 | 22 | 18 |
Bud Site | 1 | 1 | 1 | 0 | 0 | 0 | 1 | – | 3 | 0 | 4 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 13 | 9 | 17 | 16 | 9 | – | 12 | 15 | 13 | 16 | 17 | 6 | 19 | 28 | 25 | 34 | 0 | 0 | 3 | 4 | 11 | 10 |
Cytoplasm | 108 | 197 | 354 | 324 | 472 | 455 | 615 | – | 424 | 549 | 284 | 505 | 343 | 492 | 640 | 296 | 198 | 207 | 108 | 196 | 316 | 187 | 313 | 465 |
Endoplasmic Reticulum | 1 | 5 | 0 | 0 | 1 | 2 | 5 | – | 1 | 3 | 4 | 3 | 1 | 2 | 2 | 15 | 4 | 13 | 0 | 1 | 3 | 4 | 14 | 8 |
Endosome | 0 | 0 | 0 | 1 | 0 | 0 | 1 | – | 0 | 3 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 3 | 4 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 4 | 7 | 1 | 1 | 3 | 33 | 60 | – | 127 | 156 | 82 | 100 | 4 | 2 | 7 | 3 | 3 | 1 | 0 | 7 | 11 | 22 | 30 | 18 |
Nucleus | 0 | 0 | 1 | 3 | 2 | 1 | 2 | – | 4 | 4 | 3 | 4 | 0 | 0 | 1 | 3 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 4 | 1 | 4 | 6 | – | 1 | 6 | 1 | 7 | 1 | 5 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 5 |
Vac/Vac Membrane | 0 | 0 | 3 | 0 | 2 | 2 | 4 | – | 3 | 9 | 1 | 5 | 1 | 0 | 1 | 3 | 2 | 3 | 0 | 2 | 5 | 21 | 25 | 25 |
Unique Cell Count | 120 | 213 | 365 | 334 | 492 | 486 | 679 | 514 | 667 | 354 | 594 | 358 | 510 | 665 | 308 | 209 | 221 | 123 | 237 | 373 | 300 | 481 | 610 | |
Labelled Cell Count | 128 | 227 | 394 | 357 | 522 | 528 | 728 | 597 | 779 | 416 | 661 | 388 | 533 | 697 | 354 | 242 | 266 | 123 | 237 | 373 | 300 | 481 | 610 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 5.0 | 5.9 | 5.5 | 6.2 | 4.9 | 4.9 | – | 4.2 | 4.3 | 4.2 | 4.7 | 5.2 | 5.0 | 5.2 | 7.5 | 7.8 | 7.7 | 4.8 | 4.7 | 5.4 |
Std Deviation (1e-4) | 0.6 | 0.7 | 1.1 | 1.6 | 1.5 | 1.0 | 0.8 | – | 0.6 | 0.8 | 0.8 | 0.8 | 0.8 | 0.7 | 1.0 | 1.0 | 1.0 | 1.1 | 1.1 | 0.9 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.08 | -0.25 | -0.27 | – | -0.48 | -0.46 | -0.47 | -0.33 | -0.18 | -0.23 | -0.17 | 0.36 | 0.4 | 0.39 | -0.29 | -0.32 | -0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.4 | 0 | 0 | -1.5 | – | 0 | 0 | 0 | 0 | -0.2 | -0.2 | 0 | 0 | 0 | 0 |
Bud | 0.5 | 0.7 | 0 | 0.9 | – | 0 | 0 | 0 | 0 | 1.8 | 0.5 | 1.1 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | -0.8 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.7 | -0.1 | -0.2 | -2.4 | – | 0 | 0 | 0 | 0 | 0.8 | -2.4 | -0.6 | 3.0 | 3.9 | 5.1 |
Cytoplasm | 0 | -0.8 | -2.2 | -3.8 | – | -6.6 | -6.8 | -7.1 | -5.9 | -0.8 | -0.4 | -0.6 | -0.6 | -1.4 | -1.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.3 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.8 | 5.6 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.4691 | 0.6975 | 0.5535 | 0.731 | 0.1471 | 0.4358 | -0.1114 | -0.0812 | 0.3884 | 0.6774 | 0.2542 | 0.3555 | 0.2097 | 0.3632 | 0.397 | 0.5981 | 0.7109 | 0.3316 |
Actin | 0.1129 | 0.0017 | 0.0074 | 0.0206 | 0.017 | 0.0046 | 0.0098 | 0.0019 | 0.0166 | 0.0018 | 0.0029 | 0.0204 | 0.0135 | 0.0078 | 0.0453 | 0.0231 | 0.009 | 0.0048 |
Bud | 0.0079 | 0.0096 | 0.0127 | 0.0021 | 0.0006 | 0.003 | 0.0012 | 0.0055 | 0.0039 | 0.0046 | 0.0019 | 0.001 | 0.0118 | 0.0021 | 0.0553 | 0.0003 | 0.0019 | 0.0001 |
Bud Neck | 0.0026 | 0.0009 | 0.0319 | 0.0006 | 0.0005 | 0.0303 | 0.0014 | 0.0015 | 0.0022 | 0.0009 | 0.0007 | 0.0464 | 0.0009 | 0.0012 | 0.0042 | 0.0008 | 0.0246 | 0.0308 |
Bud Periphery | 0.0183 | 0.0148 | 0.0219 | 0.0084 | 0.0007 | 0.0047 | 0.0019 | 0.0095 | 0.0048 | 0.0079 | 0.0027 | 0.0014 | 0.0124 | 0.0019 | 0.0606 | 0.0002 | 0.0031 | 0.0001 |
Bud Site | 0.0154 | 0.0219 | 0.028 | 0.0013 | 0.0012 | 0.0014 | 0.0097 | 0.0294 | 0.0128 | 0.002 | 0.0019 | 0.0009 | 0.0203 | 0.0331 | 0.03 | 0.0007 | 0.0121 | 0.0001 |
Cell Periphery | 0.0022 | 0.0011 | 0.0011 | 0.0004 | 0.0002 | 0.0006 | 0.0006 | 0.001 | 0.0005 | 0.0004 | 0.0001 | 0.0003 | 0.0005 | 0.001 | 0.001 | 0.0001 | 0.0008 | 0.0002 |
Cytoplasm | 0.2705 | 0.4012 | 0.3838 | 0.5402 | 0.4893 | 0.461 | 0.59 | 0.4977 | 0.649 | 0.6209 | 0.6165 | 0.7128 | 0.5049 | 0.5869 | 0.532 | 0.6523 | 0.6595 | 0.7805 |
Cytoplasmic Foci | 0.0253 | 0.0184 | 0.0147 | 0.0173 | 0.0177 | 0.017 | 0.048 | 0.0307 | 0.0236 | 0.0307 | 0.0372 | 0.0144 | 0.0179 | 0.0147 | 0.0175 | 0.032 | 0.0225 | 0.0053 |
Eisosomes | 0.0007 | 0.0002 | 0.0007 | 0.0006 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0005 | 0.0005 | 0.0002 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0058 | 0.002 | 0.0076 | 0.005 | 0.0025 | 0.0033 | 0.0034 | 0.0013 | 0.0026 | 0.0026 | 0.0018 | 0.0023 | 0.0144 | 0.0034 | 0.0045 | 0.0023 | 0.0028 | 0.0045 |
Endosome | 0.0403 | 0.0053 | 0.0074 | 0.0128 | 0.0106 | 0.0112 | 0.0213 | 0.0024 | 0.0249 | 0.0264 | 0.0909 | 0.0094 | 0.0373 | 0.0045 | 0.0189 | 0.0439 | 0.0546 | 0.0044 |
Golgi | 0.0147 | 0.0006 | 0.0011 | 0.0027 | 0.0027 | 0.005 | 0.007 | 0.001 | 0.0071 | 0.0114 | 0.0265 | 0.0029 | 0.0043 | 0.0016 | 0.005 | 0.0132 | 0.0168 | 0.0005 |
Lipid Particles | 0.0115 | 0.001 | 0.004 | 0.0036 | 0.002 | 0.0019 | 0.0131 | 0.001 | 0.0014 | 0.0089 | 0.0012 | 0.0007 | 0.0085 | 0.001 | 0.0037 | 0.0206 | 0.021 | 0.0005 |
Mitochondria | 0.0052 | 0.0006 | 0.0035 | 0.0033 | 0.0012 | 0.0026 | 0.0139 | 0.0008 | 0.0074 | 0.0186 | 0.0129 | 0.0123 | 0.016 | 0.001 | 0.0127 | 0.0025 | 0.0096 | 0.0005 |
None | 0.4301 | 0.5131 | 0.4487 | 0.3561 | 0.4266 | 0.44 | 0.235 | 0.4073 | 0.2329 | 0.2445 | 0.1878 | 0.1604 | 0.2557 | 0.3323 | 0.1902 | 0.1781 | 0.1071 | 0.1598 |
Nuclear Periphery | 0.0038 | 0.0006 | 0.0046 | 0.0058 | 0.0049 | 0.0021 | 0.0026 | 0.0004 | 0.0007 | 0.0011 | 0.0009 | 0.006 | 0.0371 | 0.0007 | 0.0012 | 0.0023 | 0.005 | 0.0035 |
Nucleolus | 0.0004 | 0.0007 | 0.002 | 0.0007 | 0.0002 | 0.0003 | 0.0009 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0023 | 0.0003 | 0.0003 | 0.0004 | 0.0022 | 0.0002 |
Nucleus | 0.0017 | 0.0012 | 0.0056 | 0.0022 | 0.0016 | 0.0022 | 0.0034 | 0.0012 | 0.0012 | 0.002 | 0.0013 | 0.0018 | 0.0185 | 0.0014 | 0.0012 | 0.001 | 0.0046 | 0.0018 |
Peroxisomes | 0.0116 | 0.0005 | 0.0033 | 0.0082 | 0.0032 | 0.0007 | 0.0249 | 0.0032 | 0.0024 | 0.005 | 0.0021 | 0.0037 | 0.0075 | 0.0015 | 0.0042 | 0.0075 | 0.0121 | 0.0001 |
Punctate Nuclear | 0.0096 | 0.0013 | 0.0023 | 0.0058 | 0.015 | 0.0044 | 0.0057 | 0.0018 | 0.0008 | 0.0011 | 0.0005 | 0.0007 | 0.0049 | 0.0009 | 0.0012 | 0.0069 | 0.0014 | 0.0009 |
Vacuole | 0.0083 | 0.0029 | 0.0062 | 0.0019 | 0.002 | 0.003 | 0.0045 | 0.0016 | 0.0042 | 0.0069 | 0.0075 | 0.0015 | 0.0075 | 0.002 | 0.0087 | 0.0078 | 0.0231 | 0.0009 |
Vacuole Periphery | 0.0011 | 0.0003 | 0.0013 | 0.0005 | 0.0004 | 0.0007 | 0.0012 | 0.0001 | 0.0008 | 0.0019 | 0.0026 | 0.0007 | 0.0034 | 0.0002 | 0.0019 | 0.0035 | 0.0058 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.9697 | 5.3535 | 6.6208 | 8.6084 | 13.9114 | 14.4645 | 9.6981 | 8.9811 | 9.0583 | 15.6539 |
Translational Efficiency | 0.8096 | 1.8738 | 0.7804 | 0.7266 | 0.781 | 0.8974 | 0.9876 | 0.8753 | 0.8303 | 0.8526 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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