Standard name
Human Ortholog
Description Histone H4; core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.09 0 0 0 0 – 0 0.06 0 0.06 0 0 0 0.05 0.1 0 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.41 0.4 0.17 0.32 0.15 0.38 – 0.41 0.48 0.4 0.48 0.45 0.25 0.35 0.16 0.11 0.15 0.13 0 0 0 0 0 0
Nucleus 0.87 0.84 0.87 0.79 0.83 0.71 – 0.61 0.58 0.59 0.56 0.6 0.86 0.7 0.65 0.78 0.82 0.75 0.84 0.8 0.76 0.7 0.51 0.5
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.44 0.37 0.68 0.57 0.42 0.65 – 0.54 0.66 0.67 0.72 0.68 0.54 0.54 0.51 0.18 0.23 0.21 0.1 0.12 0.17 0.22 0.29 0.33
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.13 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 – 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 2 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 0 0 0 0 – 1 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0
Cytoplasm 12 16 1 6 7 11 – 17 10 8 12 8 6 5 9 23 13 25 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 2 1 2 0 1 0 0 0 0
Endosome 0 0 0 0 1 0 – 0 0 0 0 0 0 1 1 1 0 2 0 0 1 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0
Mitochondria 70 73 12 51 35 94 – 161 87 103 99 100 38 43 28 27 43 35 0 0 0 0 2 0
Nucleus 148 154 60 126 187 174 – 241 105 154 115 134 131 85 114 184 240 204 134 140 63 33 57 62
Nuclear Periphery 0 2 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 74 68 47 90 94 160 – 212 118 174 148 152 83 66 90 42 67 57 16 21 14 10 32 40
Peroxisomes 0 0 0 0 1 0 – 0 1 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 2 0 0 0 0 – 0 0 1 0 0 0 0 3 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 2 0 0 0 0 – 7 3 0 0 0 0 0 1 3 3 2 5 4 2 2 14 17
Unique Cell Count 170 183 69 159 226 245 395 180 259 206 223 153 122 175 237 293 272 160 175 83 48 112 124
Labelled Cell Count 306 319 120 273 325 440 641 324 440 374 394 259 201 246 284 367 327 160 175 83 48 112 124


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 78.5 87.0 40.5 60.5 72.0 67.7 – 71.2 67.4 60.9 63.8 59.3 70.4 67.9 58.5 80.9 80.4 75.0 67.7 64.5 61.2
Std Deviation (1e-4) 34.3 34.6 17.0 27.5 31.9 30.9 – 30.0 31.0 27.9 31.5 30.3 31.8 27.5 29.3 49.2 42.4 42.3 33.6 35.0 29.8
Intensity Change (Log2) – – – 0.58 0.83 0.74 – 0.81 0.74 0.59 0.65 0.55 0.8 0.75 0.53 1.0 0.99 0.89 0.74 0.67 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 – 0 0 0 0 0 0 0 0 2.2 0 2.2
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.3 -0.4 3.3 – 3.7 4.5 3.5 4.5 4.1 1.2 2.6 -0.3 -1.3 -0.6 -1.0
Nucleus -1.4 -0.8 -2.7 – -4.2 -4.3 -4.3 -4.7 -4.1 -0.3 -2.7 -3.4 -1.7 -1.0 -2.1
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.6 -3.9 -0.4 – -2.2 -0.4 -0.1 0.6 0 -1.9 -1.9 -2.4 -8.1 -7.3 -7.6
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – – – – –
Bud Periphery – – – – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – – – – –
Cytoplasmic Foci – – – – – – – – – – – – – – – – – –
Eisosomes – – – – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – – – – –
Lipid Particles – – – – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – – – – –
None – – – – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – – – – –
Punctate Nuclear – – – – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – – – – –
Vacuole Periphery – – – – – – – – – – – – – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1851.3853 1523.8247 300.2805 159.4995 2019.8225 940.2022 741.5998 467.0893 418.937 1294.5231
Translational Efficiency 1.7184 1.7086 1.1226 2.5869 1.5727 2.4381 1.7828 1.8759 1.3086 1.9084

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization –
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Histone H4; core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity
Localization
Cell Percentages nucleus (83%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-1

Hhf1

Hhf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hhf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available