Standard name | |
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Human Ortholog | |
Description | Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Bud | 0 | 1 | 0 | 0 | 1 | 4 | 0 | – | 5 | 2 | 6 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 7 | 1 | 1 | 2 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 67 | 237 | 119 | 140 | 150 | 179 | 321 | – | 380 | 446 | 410 | 406 | 130 | 244 | 280 | 58 | 39 | 44 | 5 | 24 | 9 | 20 | 18 | 24 |
Endoplasmic Reticulum | 3 | 2 | 2 | 1 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 15 | 18 | 11 | 0 | 2 | 1 | 2 | 2 | 4 |
Endosome | 19 | 11 | 9 | 3 | 7 | 0 | 5 | – | 2 | 4 | 2 | 5 | 3 | 6 | 2 | 5 | 14 | 5 | 16 | 20 | 3 | 4 | 10 | 13 |
Golgi | 4 | 0 | 0 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 4 | 1 | 0 | 0 | 1 |
Mitochondria | 41 | 61 | 2 | 3 | 0 | 19 | 5 | – | 14 | 18 | 18 | 40 | 1 | 0 | 0 | 10 | 6 | 10 | 5 | 9 | 3 | 2 | 10 | 10 |
Nucleus | 42 | 49 | 14 | 11 | 9 | 24 | 10 | – | 10 | 9 | 4 | 3 | 0 | 0 | 0 | 117 | 115 | 82 | 2 | 7 | 0 | 1 | 0 | 5 |
Nuclear Periphery | 1 | 3 | 0 | 1 | 0 | 1 | 0 | – | 2 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 5 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 3 | 3 | 1 | 0 | 2 | 4 |
Vac/Vac Membrane | 285 | 430 | 128 | 126 | 142 | 80 | 161 | – | 130 | 115 | 103 | 105 | 50 | 33 | 15 | 137 | 180 | 141 | 344 | 598 | 221 | 209 | 242 | 205 |
Unique Cell Count | 366 | 639 | 220 | 214 | 235 | 252 | 404 | 466 | 517 | 483 | 485 | 165 | 272 | 288 | 281 | 298 | 231 | 389 | 685 | 250 | 247 | 295 | 293 | |
Labelled Cell Count | 462 | 795 | 274 | 286 | 310 | 307 | 503 | 545 | 595 | 546 | 569 | 185 | 286 | 297 | 342 | 376 | 297 | 389 | 685 | 250 | 247 | 295 | 293 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 4.7 | 5.8 | 5.8 | 7.4 | 4.7 | 5.7 | – | 4.9 | 5.0 | 4.9 | 4.8 | 5.3 | 5.2 | 5.4 | 7.5 | 8.2 | 7.0 | 4.7 | 5.0 | 5.6 |
Std Deviation (1e-4) | 1.1 | 1.0 | 1.6 | 1.3 | 6.4 | 1.7 | 1.7 | – | 1.4 | 1.4 | 1.2 | 1.0 | 1.5 | 2.0 | 1.9 | 2.1 | 2.3 | 1.9 | 1.3 | 1.7 | 2.4 |
Intensity Change (Log2) | – | – | – | 0.0 | 0.35 | -0.3 | -0.03 | – | -0.23 | -0.21 | -0.23 | -0.25 | -0.14 | -0.15 | -0.11 | 0.39 | 0.51 | 0.27 | -0.31 | -0.22 | -0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | -2.1 | 0.5 | 0.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.2182 | 1.269 | 0.9773 | 0.5163 | 0.5387 | 1.1376 | 1.7956 | 3.6024 | 2.7615 | 2.3774 | 2.4512 | 2.7989 | 3.6608 | 5.5461 | 4.7124 | 5.0208 | 4.8643 | 5.2253 |
Actin | 0.0064 | 0 | 0.02 | 0 | 0.0001 | 0.0014 | 0.0069 | 0 | 0 | 0 | 0 | 0.0015 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Bud | 0.0005 | 0.0004 | 0.0006 | 0.0004 | 0.0004 | 0.0006 | 0.0003 | 0.0004 | 0.0004 | 0.0003 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0002 |
Bud Neck | 0.0008 | 0.001 | 0.0024 | 0.0008 | 0.0007 | 0.002 | 0.0009 | 0.0015 | 0.0014 | 0.0005 | 0.0012 | 0.0019 | 0.0005 | 0.0007 | 0.0043 | 0.0006 | 0.0011 | 0.0009 |
Bud Periphery | 0.001 | 0.0008 | 0.0014 | 0.0006 | 0.001 | 0.0015 | 0.0009 | 0.001 | 0.001 | 0.0007 | 0.0012 | 0.0012 | 0.0006 | 0.0009 | 0.0009 | 0.0005 | 0.0009 | 0.0007 |
Bud Site | 0.0011 | 0.0015 | 0.0025 | 0.004 | 0.0007 | 0.0014 | 0.0006 | 0.0013 | 0.0014 | 0.0005 | 0.001 | 0.001 | 0.0005 | 0.0006 | 0.0016 | 0.0004 | 0.0004 | 0.0005 |
Cell Periphery | 0.0011 | 0.0022 | 0.0019 | 0.0012 | 0.0009 | 0.0029 | 0.0013 | 0.0023 | 0.0016 | 0.0007 | 0.0025 | 0.0022 | 0.0018 | 0.0023 | 0.0018 | 0.0016 | 0.0037 | 0.0024 |
Cytoplasm | 0.0155 | 0.0096 | 0.0106 | 0.0085 | 0.0023 | 0.0044 | 0.0044 | 0.0043 | 0.0056 | 0.0011 | 0.0016 | 0.0033 | 0.0111 | 0.0053 | 0.0036 | 0.0022 | 0.001 | 0.0036 |
Cytoplasmic Foci | 0.0261 | 0.005 | 0.0092 | 0.0111 | 0.0028 | 0.002 | 0.0086 | 0.0068 | 0.0065 | 0.0051 | 0.0037 | 0.0068 | 0.0059 | 0.0103 | 0.0065 | 0.0047 | 0.0005 | 0.0034 |
Eisosomes | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0083 | 0.0027 | 0.0068 | 0.0021 | 0.0038 | 0.0032 | 0.0023 | 0.0032 | 0.0029 | 0.0007 | 0.0014 | 0.0022 | 0.0032 | 0.0022 | 0.0034 | 0.0009 | 0.0016 | 0.0017 |
Endosome | 0.2472 | 0.1563 | 0.2272 | 0.3802 | 0.2285 | 0.1132 | 0.2416 | 0.1303 | 0.2177 | 0.2109 | 0.1524 | 0.1973 | 0.2312 | 0.1357 | 0.2085 | 0.1173 | 0.0763 | 0.1146 |
Golgi | 0.0077 | 0.0022 | 0.0188 | 0.0061 | 0.0046 | 0.0028 | 0.0072 | 0.0029 | 0.0064 | 0.0072 | 0.0025 | 0.0045 | 0.0048 | 0.0025 | 0.0075 | 0.0013 | 0.0011 | 0.0022 |
Lipid Particles | 0.0163 | 0.0036 | 0.0267 | 0.0079 | 0.0136 | 0.0031 | 0.0163 | 0.0048 | 0.0115 | 0.0043 | 0.0072 | 0.0112 | 0.0109 | 0.0221 | 0.0201 | 0.0059 | 0.0018 | 0.0101 |
Mitochondria | 0.0065 | 0.0023 | 0.0267 | 0.0024 | 0.004 | 0.0035 | 0.018 | 0.0118 | 0.0302 | 0.0069 | 0.0042 | 0.0125 | 0.0052 | 0.0123 | 0.0136 | 0.002 | 0.0007 | 0.0176 |
None | 0.035 | 0.0005 | 0.0268 | 0.0006 | 0.0002 | 0.001 | 0.0092 | 0.0085 | 0.0014 | 0.0001 | 0.0002 | 0.0006 | 0.0149 | 0.0209 | 0.0016 | 0.0161 | 0 | 0.0024 |
Nuclear Periphery | 0.0172 | 0.008 | 0.0354 | 0.0025 | 0.0289 | 0.0178 | 0.0071 | 0.0064 | 0.0091 | 0.0015 | 0.0026 | 0.0062 | 0.008 | 0.0035 | 0.0055 | 0.0022 | 0.0029 | 0.0037 |
Nucleolus | 0.0021 | 0.0033 | 0.0071 | 0.0031 | 0.0018 | 0.0037 | 0.0032 | 0.0047 | 0.0036 | 0.0013 | 0.0029 | 0.0036 | 0.0031 | 0.0026 | 0.0028 | 0.0025 | 0.0023 | 0.0038 |
Nucleus | 0.017 | 0.0085 | 0.0141 | 0.0039 | 0.0031 | 0.0148 | 0.0064 | 0.0042 | 0.0055 | 0.0021 | 0.0037 | 0.004 | 0.0052 | 0.0023 | 0.002 | 0.0021 | 0.0031 | 0.0031 |
Peroxisomes | 0.0047 | 0.0002 | 0.0025 | 0.0011 | 0.0005 | 0.0005 | 0.0043 | 0.0008 | 0.0011 | 0.0009 | 0.0005 | 0.0015 | 0.0004 | 0.0009 | 0.0024 | 0.0005 | 0.0002 | 0.0003 |
Punctate Nuclear | 0.0054 | 0.0001 | 0.0025 | 0.0002 | 0.0005 | 0.0044 | 0.0006 | 0.0001 | 0.0009 | 0 | 0 | 0.0005 | 0.0005 | 0.0004 | 0.0002 | 0.0003 | 0 | 0.0001 |
Vacuole | 0.4345 | 0.6344 | 0.428 | 0.4788 | 0.5957 | 0.6289 | 0.4919 | 0.6301 | 0.52 | 0.5952 | 0.6581 | 0.559 | 0.5368 | 0.6083 | 0.5533 | 0.6698 | 0.7564 | 0.6289 |
Vacuole Periphery | 0.1454 | 0.1572 | 0.1286 | 0.0843 | 0.1059 | 0.1867 | 0.1677 | 0.1747 | 0.1718 | 0.16 | 0.1527 | 0.1784 | 0.155 | 0.1658 | 0.16 | 0.1691 | 0.1457 | 0.1997 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 27.0442 | 37.0697 | 16.7436 | 28.0224 | 24.5448 | 28.8312 | 34.6293 | 41.2455 | 39.3278 | 36.712 |
Translational Efficiency | 2.1412 | 1.625 | 2.3275 | 1.566 | 1.6031 | 2.2085 | 2.0878 | 1.2791 | 1.4894 | 1.3785 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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