Standard name | |
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Human Ortholog | |
Description | P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; null mutant displays increased translation rate and increased readthrough of premature stop codons; protein abundance increases in response to hydrogen peroxide and to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 3 | 4 | 18 | 12 | 21 | 29 | 11 | 24 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Bud | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 1 | 1 | 2 | 0 | 4 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 3 | 6 | 5 | 12 | 30 | 23 | 42 | 34 | 17 | 33 | 6 | 7 | 8 | 8 | 4 | 6 | 0 | 0 | 2 | 0 | 0 | 0 |
Cytoplasm | 181 | 273 | 512 | 205 | 297 | 357 | 356 | 282 | 229 | 245 | 144 | 192 | 294 | 255 | 284 | 580 | 480 | 388 | 180 | 254 | 503 | 163 | 179 | 166 |
Endoplasmic Reticulum | 2 | 2 | 9 | 5 | 24 | 22 | 69 | 76 | 77 | 59 | 40 | 50 | 3 | 2 | 2 | 35 | 17 | 16 | 0 | 0 | 17 | 2 | 2 | 5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 5 | 4 | 2 | 5 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 1 | 10 | 22 | 12 | 11 | 37 | 29 | 31 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 2 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 4 | 3 | 4 | 0 | 1 | 0 | 0 | 0 | 2 | 4 | 2 | 0 | 4 | 0 | 0 | 1 | 1 |
Unique Cell Count | 183 | 275 | 521 | 212 | 326 | 391 | 464 | 391 | 348 | 374 | 217 | 294 | 296 | 257 | 288 | 585 | 487 | 395 | 184 | 265 | 531 | 171 | 198 | 185 |
Labelled Cell Count | 183 | 277 | 526 | 217 | 331 | 408 | 501 | 420 | 389 | 414 | 243 | 342 | 303 | 264 | 297 | 625 | 509 | 413 | 184 | 265 | 531 | 171 | 198 | 185 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 54.2 | 73.0 | 61.2 | 54.2 | 42.1 | 37.7 | 33.9 | 32.5 | 32.0 | 30.7 | 30.2 | 28.9 | 75.0 | 72.5 | 67.7 | 109.6 | 97.5 | 113.4 | 76.6 | 74.2 | 86.4 |
Std Deviation (1e-4) | 10.2 | 14.9 | 11.1 | 10.8 | 8.2 | 7.9 | 7.5 | 8.3 | 7.5 | 7.6 | 8.2 | 7.4 | 16.7 | 16.8 | 15.2 | 35.0 | 29.9 | 30.6 | 17.9 | 25.8 | 25.4 |
Intensity Change (Log2) | – | – | – | -0.18 | -0.54 | -0.7 | -0.85 | -0.91 | -0.94 | -1.0 | -1.02 | -1.09 | 0.29 | 0.24 | 0.14 | 0.84 | 0.67 | 0.89 | 0.32 | 0.28 | 0.5 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 4.5 | 4.0 | 5.7 | 6.5 | 0 | 6.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 2.9 | 5.1 | 4.8 | 7.5 | 6.3 | 5.5 | 7.1 | 0 | 0 | 0 | 1.3 | 0 | 0 |
Cytoplasm | -1.3 | -4.9 | -4.9 | -10.4 | -11.6 | -13.2 | -13.4 | -12.6 | -13.2 | 1.3 | 1.1 | 0.4 | 1.3 | 0.4 | -0.1 |
Endoplasmic Reticulum | 0 | 4.1 | 3.2 | 7.6 | 9.1 | 9.9 | 7.8 | 8.3 | 8.1 | 0 | 0 | 0 | 3.6 | 1.8 | 2.1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 5.0 | 4.0 | 0 | 7.3 | 8.5 | 7.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 48.9565 | 59.6847 | 59.7471 | 50.0162 | 54.4517 | 55.0058 | 44.61 | 69.0708 | 61.8508 | 52.2237 | 49.9745 | 66.4469 | 55.2123 | 70.9707 | 64.6447 | 62.8335 | 62.9644 | 66.7863 |
Actin | 0.0067 | 0.0001 | 0.0009 | 0.0001 | 0.0002 | 0.0007 | 0.0047 | 0.0003 | 0.0056 | 0.0017 | 0.0002 | 0.0046 | 0.0112 | 0.0002 | 0.0025 | 0.0002 | 0.0059 | 0.0014 |
Bud | 0.0018 | 0.0002 | 0.0003 | 0.0004 | 0.0005 | 0.0005 | 0.0026 | 0.0003 | 0.0007 | 0.0006 | 0.0006 | 0.0002 | 0.0008 | 0.0003 | 0.0007 | 0.0009 | 0.0008 | 0.0005 |
Bud Neck | 0.0006 | 0.0002 | 0.0003 | 0.0004 | 0.0007 | 0.001 | 0.0006 | 0.0004 | 0.0004 | 0.0005 | 0.0008 | 0.0013 | 0.0005 | 0.0003 | 0.0009 | 0.0005 | 0.0005 | 0.0013 |
Bud Periphery | 0.0015 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0039 | 0.0002 | 0.001 | 0.0005 | 0.0009 | 0.0002 | 0.0004 | 0.0002 | 0.0006 | 0.0004 | 0.0019 | 0.0006 |
Bud Site | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.002 | 0.0004 | 0.0004 | 0.001 | 0.0023 | 0.0001 | 0.0005 | 0.0002 | 0.002 | 0.0002 | 0.0008 | 0.0004 |
Cell Periphery | 0.0051 | 0.0023 | 0.0031 | 0.0016 | 0.0026 | 0.0006 | 0.0152 | 0.0171 | 0.0112 | 0.0105 | 0.0053 | 0.005 | 0.0041 | 0.0049 | 0.0059 | 0.0009 | 0.0007 | 0.0024 |
Cytoplasm | 0.8092 | 0.962 | 0.9166 | 0.8951 | 0.8528 | 0.9234 | 0.6865 | 0.9314 | 0.8681 | 0.7675 | 0.8251 | 0.8875 | 0.8237 | 0.9648 | 0.894 | 0.8603 | 0.9094 | 0.9396 |
Cytoplasmic Foci | 0.0068 | 0.0009 | 0.0025 | 0.004 | 0.0085 | 0.0031 | 0.0112 | 0.0007 | 0.0061 | 0.0127 | 0.0104 | 0.0037 | 0.0028 | 0.0009 | 0.0022 | 0.0013 | 0.0026 | 0.0056 |
Eisosomes | 0.001 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0533 | 0.0156 | 0.0214 | 0.0052 | 0.0113 | 0.0329 | 0.0597 | 0.0108 | 0.0093 | 0.0066 | 0.0057 | 0.0625 | 0.0577 | 0.007 | 0.0148 | 0.0039 | 0.0013 | 0.0124 |
Endosome | 0.0094 | 0.0012 | 0.0064 | 0.0086 | 0.0183 | 0.0089 | 0.022 | 0.0007 | 0.0037 | 0.0145 | 0.0164 | 0.0043 | 0.0071 | 0.0014 | 0.0056 | 0.0052 | 0.0156 | 0.0087 |
Golgi | 0.0018 | 0.0001 | 0.0019 | 0.0004 | 0.0019 | 0.0025 | 0.0031 | 0.0004 | 0.0006 | 0.0025 | 0.0027 | 0.0008 | 0.0007 | 0.0006 | 0.0055 | 0.0001 | 0.0075 | 0.0018 |
Lipid Particles | 0.0023 | 0.0006 | 0.0019 | 0.0036 | 0.0034 | 0.0002 | 0.012 | 0.0015 | 0.0039 | 0.0116 | 0.0023 | 0.0015 | 0.0006 | 0.001 | 0.0014 | 0.0001 | 0.0048 | 0.0021 |
Mitochondria | 0.0043 | 0.0001 | 0.0009 | 0.0001 | 0.0011 | 0.0029 | 0.0108 | 0.0001 | 0.0076 | 0.0082 | 0.0039 | 0.0007 | 0.0013 | 0.0024 | 0.0025 | 0.0005 | 0.0169 | 0.0024 |
None | 0.0034 | 0.0002 | 0.001 | 0.0003 | 0.0017 | 0.0003 | 0.0175 | 0.0003 | 0.0018 | 0.0074 | 0.011 | 0.0011 | 0.0029 | 0.0002 | 0.0037 | 0.0005 | 0.0004 | 0.0007 |
Nuclear Periphery | 0.0093 | 0.0009 | 0.0038 | 0.0027 | 0.0039 | 0.002 | 0.02 | 0.0018 | 0.006 | 0.0116 | 0.0048 | 0.0038 | 0.0126 | 0.0011 | 0.0042 | 0.0181 | 0.0016 | 0.0037 |
Nucleolus | 0.0028 | 0 | 0.0002 | 0.0001 | 0.0104 | 0.0001 | 0.0073 | 0.0002 | 0.0012 | 0.0078 | 0.0089 | 0.0002 | 0.0027 | 0 | 0.0038 | 0.0002 | 0.0002 | 0.0005 |
Nucleus | 0.0094 | 0.0008 | 0.0015 | 0.0015 | 0.0106 | 0.0014 | 0.0165 | 0.0015 | 0.0034 | 0.0069 | 0.0128 | 0.0019 | 0.0152 | 0.0009 | 0.0065 | 0.013 | 0.0013 | 0.0024 |
Peroxisomes | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0015 | 0 | 0.0008 | 0.0014 | 0.0011 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0013 | 0.0001 |
Punctate Nuclear | 0.0009 | 0 | 0.0007 | 0.0001 | 0.0017 | 0 | 0.0054 | 0 | 0.0006 | 0.007 | 0.0009 | 0.0064 | 0.0032 | 0 | 0.001 | 0.0002 | 0.0002 | 0.0003 |
Vacuole | 0.0583 | 0.0138 | 0.0326 | 0.0675 | 0.0607 | 0.0117 | 0.0843 | 0.0314 | 0.0623 | 0.1088 | 0.074 | 0.0124 | 0.0458 | 0.0125 | 0.0344 | 0.0841 | 0.0226 | 0.0101 |
Vacuole Periphery | 0.0113 | 0.0007 | 0.0035 | 0.0081 | 0.0092 | 0.0072 | 0.0126 | 0.0007 | 0.0053 | 0.0106 | 0.0098 | 0.0014 | 0.0062 | 0.0009 | 0.0076 | 0.0093 | 0.0036 | 0.003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 331.4869 | 341.2778 | 384.188 | 418.5677 | 424.3546 | 276.3964 | 315.188 | 323.2143 | 352.8491 | 347.8165 |
Translational Efficiency | 1.8066 | 1.9176 | 1.7151 | 1.7353 | 1.7336 | 2.0615 | 1.8758 | 1.6666 | 1.6562 | 1.6998 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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