Standard name | |
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Human Ortholog | |
Description | Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; involved in spore wall asembly; sequence similarity to DTR1 and QDR1, and the triple mutant dtr1 qdr1 qdr3 exhibits reduced dityrosine fluorescence relative to the single mutants; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 27 | 64 | 40 | 31 | 35 | 88 | 76 | 61 | 31 | 30 | 41 | 24 | – | – | – | 21 | 14 | 10 | 0 | 4 | 4 | 0 | 1 | 0 |
Bud | 3 | 5 | 0 | 1 | 3 | 9 | 11 | 20 | 8 | 13 | 17 | 17 | – | – | – | 2 | 2 | 3 | 1 | 1 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 193 | 181 | 197 | 139 | 206 | 184 | 194 | 165 | 93 | 111 | 112 | 143 | – | – | – | 213 | 128 | 162 | 224 | 202 | 239 | 7 | 17 | 8 |
Cytoplasm | 8 | 24 | 21 | 17 | 23 | 42 | 93 | 129 | 97 | 131 | 112 | 156 | – | – | – | 46 | 30 | 34 | 2 | 5 | 2 | 0 | 0 | 0 |
Endoplasmic Reticulum | 4 | 2 | 4 | 3 | 29 | 84 | 137 | 100 | 71 | 56 | 69 | 53 | – | – | – | 6 | 2 | 5 | 0 | 2 | 6 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 3 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1 | 3 | 1 | 0 | 2 | 3 | 4 | 1 | 3 | 8 | 3 | – | – | – | 8 | 18 | 29 | 1 | 4 | 5 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 2 | 13 | 15 | 8 | 19 | 18 | 19 | – | – | – | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 2 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 4 | 2 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 3 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 1 | 0 | 0 | 5 | 22 | 52 | 27 | 39 | 42 | 41 | – | – | – | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Unique Cell Count | 206 | 191 | 225 | 163 | 247 | 292 | 407 | 412 | 259 | 300 | 312 | 342 | 249 | 163 | 196 | 231 | 226 | 263 | 9 | 21 | 11 | |||
Labelled Cell Count | 236 | 277 | 267 | 193 | 297 | 422 | 558 | 552 | 338 | 407 | 425 | 463 | 299 | 197 | 247 | 231 | 226 | 263 | 9 | 21 | 11 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 34.0 | 26.5 | 30.7 | 31.1 | 28.1 | 22.4 | 21.4 | 20.5 | 20.5 | 19.4 | 20.0 | 21.0 | – | – | – | 16.6 | 18.6 | 24.1 | – | – | – |
Std Deviation (1e-4) | 7.2 | 6.2 | 7.9 | 7.8 | 7.7 | 8.1 | 7.5 | 6.5 | 6.6 | 6.2 | 6.3 | 5.9 | – | – | – | 4.6 | 5.4 | 6.4 | – | – | – |
Intensity Change (Log2) | – | – | – | 0.02 | -0.13 | -0.45 | -0.52 | -0.58 | -0.58 | -0.66 | -0.62 | -0.55 | – | – | – | -0.88 | -0.72 | -0.35 | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.3 | -1.1 | 3.2 | 0.3 | -1.0 | -1.8 | -2.6 | -1.5 | -4.0 | – | – | – | -3.0 | -2.6 | -4.0 |
Bud | 0 | 0 | 0 | 0 | 3.4 | 0 | 3.2 | 3.6 | 3.4 | – | – | – | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Cell Periphery | -0.7 | -1.3 | -6.3 | -9.9 | -11.6 | -11.6 | -11.6 | -11.9 | -10.9 | – | – | – | -0.6 | -2.4 | -1.4 |
Cytoplasm | 0.4 | 0 | 1.7 | 4.2 | 6.2 | 7.2 | 8.6 | 7.0 | 9.1 | – | – | – | 2.9 | 2.6 | 2.4 |
Endoplasmic Reticulum | 0 | 4.2 | 8.1 | 9.2 | 7.3 | 7.8 | 6.0 | 6.8 | 5.3 | – | – | – | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 1.4 | 4.2 | 5.2 |
Nucleus | 0 | 0 | 0 | 0 | 2.5 | 0 | 3.5 | 3.3 | 3.2 | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 3.2 | 5.3 | 4.7 | 5.4 | 5.5 | 5.1 | – | – | – | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.9922 | 4.3912 | 3.6005 | 3.6327 | 2.992 | 4.0719 | 2.5409 | 3.4639 | 3.4565 | 3.2287 | 2.321 | 4.1186 | 3.4751 | 4.8007 | 3.8987 | 3.3311 | 3.0846 | 4.0224 |
Actin | 0.0314 | 0.0011 | 0.0256 | 0.0551 | 0.0687 | 0.0092 | 0.006 | 0.0119 | 0.0005 | 0.0004 | 0.0286 | 0.0026 | 0.0612 | 0.0062 | 0.0247 | 0.0016 | 0.0305 | 0.0069 |
Bud | 0.0024 | 0.0002 | 0.001 | 0.0009 | 0.0046 | 0.0009 | 0.0003 | 0.001 | 0.0003 | 0.0003 | 0.0013 | 0.0004 | 0.0013 | 0.0002 | 0.0004 | 0.0004 | 0.0024 | 0.0014 |
Bud Neck | 0.01 | 0.0023 | 0.0325 | 0.0081 | 0.0113 | 0.0263 | 0.0008 | 0.0023 | 0.0149 | 0.0139 | 0.0109 | 0.0475 | 0.007 | 0.0027 | 0.0121 | 0.037 | 0.0153 | 0.0106 |
Bud Periphery | 0.0134 | 0.001 | 0.0067 | 0.0111 | 0.0163 | 0.011 | 0.0006 | 0.0055 | 0.0032 | 0.0041 | 0.0184 | 0.0068 | 0.0085 | 0.0018 | 0.0022 | 0.0066 | 0.0162 | 0.0057 |
Bud Site | 0.0272 | 0.0614 | 0.0461 | 0.005 | 0.0093 | 0.0023 | 0.0052 | 0.034 | 0.0243 | 0.0026 | 0.0088 | 0.0013 | 0.0199 | 0.0799 | 0.0457 | 0.0132 | 0.0178 | 0.0016 |
Cell Periphery | 0.5565 | 0.5337 | 0.5615 | 0.5733 | 0.4736 | 0.6736 | 0.2798 | 0.5001 | 0.6564 | 0.7399 | 0.4818 | 0.7309 | 0.5928 | 0.5886 | 0.6938 | 0.6815 | 0.5967 | 0.7814 |
Cytoplasm | 0.2239 | 0.3647 | 0.2699 | 0.2738 | 0.2002 | 0.2235 | 0.5069 | 0.4245 | 0.2758 | 0.2315 | 0.2337 | 0.168 | 0.2253 | 0.2874 | 0.1891 | 0.2509 | 0.2164 | 0.1662 |
Cytoplasmic Foci | 0.0102 | 0.001 | 0.0045 | 0.0021 | 0.0142 | 0.0014 | 0.0155 | 0.0007 | 0.0004 | 0.0004 | 0.0362 | 0.0003 | 0.0048 | 0.0051 | 0.0032 | 0.0003 | 0.0063 | 0.0071 |
Eisosomes | 0.0063 | 0.0018 | 0.0059 | 0.0013 | 0.0219 | 0.0026 | 0.002 | 0.0028 | 0.0009 | 0.0002 | 0.0071 | 0.0004 | 0.021 | 0.0017 | 0.0043 | 0.0007 | 0.0034 | 0.0003 |
Endoplasmic Reticulum | 0.0112 | 0.0094 | 0.0047 | 0.0097 | 0.0143 | 0.0063 | 0.0327 | 0.006 | 0.0025 | 0.0019 | 0.0068 | 0.002 | 0.0077 | 0.0032 | 0.0068 | 0.0031 | 0.0066 | 0.0026 |
Endosome | 0.014 | 0.001 | 0.0049 | 0.0017 | 0.0259 | 0.0014 | 0.055 | 0.0003 | 0 | 0 | 0.0376 | 0.0005 | 0.0051 | 0.0032 | 0.0011 | 0 | 0.021 | 0.0006 |
Golgi | 0.0052 | 0.0003 | 0.0045 | 0.0027 | 0.0166 | 0.0015 | 0.0118 | 0.0009 | 0.0001 | 0.0001 | 0.0479 | 0.0009 | 0.0067 | 0.0034 | 0.003 | 0.0002 | 0.0089 | 0.0011 |
Lipid Particles | 0.0066 | 0.0027 | 0.0036 | 0.0097 | 0.0235 | 0.0044 | 0.0083 | 0.0041 | 0.0006 | 0.0004 | 0.0516 | 0.002 | 0.0056 | 0.0056 | 0.0021 | 0.0005 | 0.0056 | 0.0017 |
Mitochondria | 0.0044 | 0 | 0.0037 | 0.0079 | 0.0131 | 0.0029 | 0.0058 | 0.0001 | 0 | 0 | 0.0113 | 0.027 | 0.0032 | 0.0006 | 0.001 | 0 | 0.0085 | 0.0002 |
None | 0.0386 | 0.0069 | 0.0054 | 0.0038 | 0.0383 | 0.0129 | 0.0399 | 0.001 | 0.0167 | 0.0013 | 0.0031 | 0.0018 | 0.009 | 0.0024 | 0.0015 | 0.0011 | 0.0039 | 0.0024 |
Nuclear Periphery | 0.0128 | 0.0005 | 0.0031 | 0.0004 | 0.0114 | 0.003 | 0.0055 | 0.0004 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0085 | 0.0003 | 0.0002 | 0.0001 | 0.0117 | 0.0001 |
Nucleolus | 0.0006 | 0.0001 | 0.0014 | 0.0005 | 0.0009 | 0.0009 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0011 | 0.0001 |
Nucleus | 0.0085 | 0.0003 | 0.0047 | 0.0005 | 0.0038 | 0.0013 | 0.0022 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0017 | 0.0002 | 0.0002 | 0.0002 | 0.0053 | 0.0002 |
Peroxisomes | 0.0037 | 0 | 0.0017 | 0.0045 | 0.0072 | 0.0008 | 0.0006 | 0.0001 | 0 | 0 | 0.0076 | 0.0007 | 0.0037 | 0.0021 | 0.0055 | 0 | 0.0014 | 0.0012 |
Punctate Nuclear | 0.0019 | 0.0001 | 0.0021 | 0.0003 | 0.0093 | 0.0028 | 0.0031 | 0.0001 | 0 | 0 | 0.0004 | 0 | 0.0014 | 0.0001 | 0.0002 | 0 | 0.0116 | 0.0013 |
Vacuole | 0.0097 | 0.0114 | 0.006 | 0.0274 | 0.0141 | 0.0109 | 0.017 | 0.0039 | 0.003 | 0.0027 | 0.0058 | 0.0064 | 0.0046 | 0.0053 | 0.0027 | 0.0024 | 0.0081 | 0.0072 |
Vacuole Periphery | 0.0013 | 0.0001 | 0.0004 | 0.0003 | 0.0016 | 0.0002 | 0.001 | 0.0001 | 0 | 0 | 0.0005 | 0.0002 | 0.0006 | 0 | 0 | 0 | 0.0013 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.9935 | 25.255 | 21.2155 | 21.239 | 17.6235 | 20.7773 | 29.2693 | 27.803 | 27.1588 | 26.4123 |
Translational Efficiency | 0.3444 | 0.3361 | 0.3271 | 0.2952 | 0.3049 | 0.2796 | 0.3045 | 0.2507 | 0.2331 | 0.2306 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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