Standard name | |
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Human Ortholog | |
Description | NADPH-dependent quinone reductase; GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Bud | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 4 | 4 | 2 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 1 | 2 | 5 | 9 | 8 | 4 | 5 | 10 | 5 | 10 | 0 | 5 | 3 | 15 | 6 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 316 | 65 | 76 | 201 | 313 | 292 | 390 | 327 | 209 | 248 | 170 | 140 | 304 | 389 | 456 | 309 | 116 | 318 | 309 | 73 | 96 | 242 | 365 | 390 |
Endoplasmic Reticulum | 2 | 0 | 2 | 1 | 5 | 1 | 8 | 4 | 2 | 7 | 3 | 2 | 1 | 1 | 4 | 13 | 3 | 15 | 0 | 0 | 2 | 3 | 1 | 3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 1 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 5 | 1 | 3 | 0 | 0 | 0 | 1 | 0 | 0 |
Mitochondria | 2 | 1 | 1 | 1 | 5 | 14 | 39 | 29 | 53 | 24 | 64 | 34 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 2 |
Nucleus | 1 | 0 | 2 | 2 | 2 | 2 | 10 | 16 | 6 | 13 | 10 | 6 | 2 | 1 | 5 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 0 | 2 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 |
Vac/Vac Membrane | 1 | 0 | 1 | 17 | 26 | 43 | 97 | 86 | 75 | 54 | 44 | 73 | 0 | 6 | 2 | 9 | 0 | 4 | 1 | 1 | 2 | 1 | 0 | 3 |
Unique Cell Count | 321 | 66 | 81 | 213 | 339 | 342 | 518 | 432 | 320 | 337 | 260 | 249 | 307 | 392 | 463 | 322 | 119 | 328 | 324 | 78 | 111 | 258 | 384 | 419 |
Labelled Cell Count | 325 | 66 | 83 | 225 | 356 | 364 | 558 | 472 | 355 | 360 | 302 | 269 | 308 | 404 | 470 | 353 | 128 | 350 | 324 | 78 | 111 | 258 | 384 | 419 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.1 | 9.9 | 9.6 | 9.5 | 10.0 | 8.9 | 8.7 | 9.1 | 8.3 | 8.7 | 8.1 | 8.9 | 12.5 | 13.2 | 14.8 | 34.9 | 34.8 | 33.7 | 9.8 | 9.7 | 9.7 |
Std Deviation (1e-4) | 1.5 | 2.3 | 1.4 | 2.0 | 1.9 | 1.6 | 1.7 | 2.0 | 2.0 | 1.9 | 2.0 | 2.3 | 2.8 | 3.1 | 3.4 | 8.2 | 8.4 | 9.3 | 2.5 | 2.4 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.06 | -0.11 | -0.15 | -0.08 | -0.21 | -0.14 | -0.25 | -0.11 | 0.38 | 0.46 | 0.63 | 1.86 | 1.86 | 1.81 | 0.03 | 0.01 | 0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.2 | -0.5 | -2.0 | -3.7 | -3.6 | -5.1 | -3.9 | -5.0 | -6.2 | 2.9 | 3.4 | 2.6 | 0.8 | 1.3 | 1.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 2.1 | 0 | 3.6 | 0 | 4.7 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 2.1 | 3.0 | 4.0 | 4.1 | 4.6 | 3.5 | 3.6 | 5.3 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.4095 | 10.7765 | 8.8535 | 8.3409 | 7.0611 | 9.6133 | 5.0225 | 7.7821 | 6.1407 | 5.0498 | 4.1391 | 6.6258 | 7.3213 | 10.2169 | 8.6037 | 7.4089 | 6.9006 | 8.9917 |
Actin | 0.009 | 0.0011 | 0.0049 | 0.0338 | 0.0084 | 0.0011 | 0.0273 | 0.004 | 0.0371 | 0.0372 | 0.0266 | 0.0083 | 0.0189 | 0.0001 | 0.0186 | 0.0259 | 0.0723 | 0.0085 |
Bud | 0.0137 | 0.0056 | 0.0163 | 0.0016 | 0.002 | 0.0094 | 0.0005 | 0.0035 | 0.0007 | 0.0006 | 0.0006 | 0.0004 | 0.0007 | 0.0001 | 0.0009 | 0.0007 | 0.002 | 0.0003 |
Bud Neck | 0.0014 | 0.0002 | 0.0021 | 0.0009 | 0.0024 | 0.0013 | 0.0012 | 0.0003 | 0.0033 | 0.0233 | 0.0015 | 0.0013 | 0.0017 | 0.0001 | 0.0009 | 0.0016 | 0.0021 | 0.0009 |
Bud Periphery | 0.0045 | 0.001 | 0.0051 | 0.0023 | 0.0041 | 0.0042 | 0.0005 | 0.0007 | 0.0006 | 0.0004 | 0.0006 | 0.0004 | 0.0005 | 0 | 0.0008 | 0.0003 | 0.0023 | 0.0002 |
Bud Site | 0.006 | 0.0029 | 0.0205 | 0.0066 | 0.0014 | 0.0007 | 0.0014 | 0.0062 | 0.0031 | 0.0017 | 0.002 | 0.0002 | 0.0011 | 0.0002 | 0.0022 | 0.0017 | 0.0027 | 0.0004 |
Cell Periphery | 0.0005 | 0.0001 | 0.0006 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 |
Cytoplasm | 0.7118 | 0.9519 | 0.8128 | 0.6383 | 0.7987 | 0.9185 | 0.7504 | 0.9538 | 0.8828 | 0.8357 | 0.545 | 0.9279 | 0.8222 | 0.9884 | 0.9122 | 0.8501 | 0.6368 | 0.921 |
Cytoplasmic Foci | 0.0311 | 0.004 | 0.0257 | 0.0293 | 0.0054 | 0.0096 | 0.0363 | 0.0045 | 0.0097 | 0.0142 | 0.0805 | 0.0088 | 0.0486 | 0.0009 | 0.0089 | 0.0509 | 0.0251 | 0.0135 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0085 | 0.0015 | 0.0085 | 0.0038 | 0.0161 | 0.0021 | 0.0062 | 0.0011 | 0.0038 | 0.0023 | 0.0064 | 0.0035 | 0.0056 | 0.0007 | 0.0023 | 0.0026 | 0.0049 | 0.0017 |
Endosome | 0.0722 | 0.0016 | 0.0174 | 0.0925 | 0.0328 | 0.0036 | 0.0369 | 0.0051 | 0.0102 | 0.0283 | 0.0532 | 0.0063 | 0.0218 | 0.0003 | 0.0143 | 0.0073 | 0.0478 | 0.0121 |
Golgi | 0.005 | 0.0001 | 0.0048 | 0.0526 | 0.0051 | 0.0002 | 0.0132 | 0.0018 | 0.0032 | 0.0116 | 0.0188 | 0.0021 | 0.0052 | 0 | 0.0044 | 0.0037 | 0.0279 | 0.0041 |
Lipid Particles | 0.0163 | 0.0003 | 0.0012 | 0.0576 | 0.0011 | 0.0001 | 0.0236 | 0.001 | 0.0011 | 0.0018 | 0.0569 | 0.0016 | 0.0197 | 0 | 0.0087 | 0.0072 | 0.0266 | 0.0024 |
Mitochondria | 0.0044 | 0.0004 | 0.0098 | 0.0251 | 0.0093 | 0.0005 | 0.0038 | 0.0005 | 0.0014 | 0.0029 | 0.0171 | 0.0041 | 0.0039 | 0 | 0.0021 | 0.0007 | 0.0591 | 0.0008 |
None | 0.0196 | 0.0056 | 0.0188 | 0.0055 | 0.0033 | 0.004 | 0.0356 | 0.0036 | 0.0144 | 0.0216 | 0.0077 | 0.0073 | 0.0136 | 0.0039 | 0.0033 | 0.0055 | 0.0024 | 0.0014 |
Nuclear Periphery | 0.0135 | 0.0023 | 0.0062 | 0.0077 | 0.042 | 0.0036 | 0.0111 | 0.0013 | 0.0098 | 0.002 | 0.0481 | 0.0062 | 0.0024 | 0.0008 | 0.0034 | 0.0027 | 0.0147 | 0.0029 |
Nucleolus | 0.0024 | 0.0001 | 0.0014 | 0.0006 | 0.0005 | 0.0001 | 0.0011 | 0.0001 | 0.0001 | 0.0001 | 0.0011 | 0.0001 | 0.0007 | 0 | 0.0002 | 0.0001 | 0.0004 | 0 |
Nucleus | 0.0408 | 0.0181 | 0.0336 | 0.0138 | 0.046 | 0.0313 | 0.014 | 0.0092 | 0.0127 | 0.0117 | 0.0183 | 0.0153 | 0.0089 | 0.0041 | 0.0078 | 0.0153 | 0.0074 | 0.023 |
Peroxisomes | 0.0033 | 0.0001 | 0.0032 | 0.0136 | 0.0002 | 0.0007 | 0.0209 | 0.0011 | 0.0016 | 0.0021 | 0.0357 | 0.0009 | 0.017 | 0 | 0.0013 | 0.0154 | 0.0565 | 0.004 |
Punctate Nuclear | 0.0168 | 0.0009 | 0.0041 | 0.0058 | 0.002 | 0.0075 | 0.0119 | 0.0012 | 0.0022 | 0.0009 | 0.038 | 0.0027 | 0.0044 | 0.0002 | 0.0028 | 0.0054 | 0.003 | 0.0013 |
Vacuole | 0.016 | 0.0019 | 0.0022 | 0.0053 | 0.0107 | 0.0012 | 0.0025 | 0.0008 | 0.0013 | 0.0009 | 0.0245 | 0.0017 | 0.0023 | 0.0002 | 0.0034 | 0.0024 | 0.0026 | 0.0012 |
Vacuole Periphery | 0.003 | 0.0002 | 0.0007 | 0.0024 | 0.0082 | 0.0003 | 0.0012 | 0.0001 | 0.0007 | 0.0005 | 0.017 | 0.0007 | 0.0005 | 0 | 0.0014 | 0.0004 | 0.0028 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 32.3885 | 19.6294 | 28.4034 | 32.2546 | 18.5054 | 36.2111 | 55.3242 | 56.9311 | 54.7153 | 34.7889 |
Translational Efficiency | 2.7499 | 3.506 | 2.2406 | 2.7216 | 2.0804 | 3.6523 | 2.3932 | 2.0737 | 2.2796 | 2.042 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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